FastQCFastQC Report
Sat 18 Jun 2016
SRR3550414_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550414_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1664734
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC34050.2045371813154534No Hit
GCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC28040.16843531759428232No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTC26540.15942486907818304No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23140.1390011857750247No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGC20270.12176119428088812No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCG17960.10788510356609525No Hit
CTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGCT16800.10091702338031182No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATCG207.0342E-445.041
CCTACGC207.0342E-445.024
ACGATTG302.1660908E-644.9999961
ATACCGG650.041.5384642
CGTAAGG1500.040.52
ACCGGAC451.9292202E-840.017
ACGTACG403.4594632E-739.3751
AGTTACG1000.038.2500041
AGGTACG659.094947E-1238.0769271
TCGTTAG900.037.51
ACGATAG601.5643309E-1037.4999961
ACCGTTA301.14014205E-437.4999969
GCTAACG601.5643309E-1037.4999961
TGTAGCG1750.037.2857131
CGAACGT552.750312E-936.8181819
GTTACGG2100.036.428572
TACGGGA4150.036.32534
GGTACGA250.00210737936.0000049
TAGCGTA250.00210737936.0000041
CGCATAT250.00210737936.00000436
Produced by FastQC (version 0.11.5)