##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550414_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1664734
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22534170624256	33.0	31.0	34.0	30.0	34.0
2	32.40015702208281	34.0	31.0	34.0	30.0	34.0
3	32.3978743751254	34.0	31.0	34.0	30.0	34.0
4	35.928291246529476	37.0	35.0	37.0	35.0	37.0
5	35.95790859080189	37.0	35.0	37.0	35.0	37.0
6	35.96262045467925	37.0	35.0	37.0	35.0	37.0
7	36.02220955419905	37.0	35.0	37.0	35.0	37.0
8	35.98189260266205	37.0	35.0	37.0	35.0	37.0
9	37.44015560443891	39.0	37.0	39.0	35.0	39.0
10	37.36024433933589	39.0	37.0	39.0	34.0	39.0
11	37.26019051692342	39.0	37.0	39.0	34.0	39.0
12	37.11252188037248	39.0	37.0	39.0	34.0	39.0
13	37.05801707660203	39.0	37.0	39.0	33.0	39.0
14	38.25087010897837	40.0	37.0	41.0	33.0	41.0
15	38.29605330341064	40.0	37.0	41.0	34.0	41.0
16	38.34074873222989	40.0	37.0	41.0	34.0	41.0
17	38.300658243298926	40.0	37.0	41.0	34.0	41.0
18	38.23255727341425	40.0	37.0	41.0	34.0	41.0
19	38.1918949213508	40.0	37.0	41.0	34.0	41.0
20	38.07139759264844	40.0	37.0	41.0	34.0	41.0
21	37.97956430276549	40.0	36.0	41.0	33.0	41.0
22	37.97271395910698	40.0	36.0	41.0	33.0	41.0
23	37.98977013745139	40.0	36.0	41.0	34.0	41.0
24	37.98762985558053	40.0	36.0	41.0	34.0	41.0
25	37.927055012993065	40.0	36.0	41.0	33.0	41.0
26	37.87304638458757	40.0	36.0	41.0	33.0	41.0
27	37.811881057274014	40.0	36.0	41.0	33.0	41.0
28	37.73684324342508	40.0	36.0	41.0	33.0	41.0
29	37.724336140188164	40.0	36.0	41.0	33.0	41.0
30	37.64723313153933	40.0	36.0	41.0	33.0	41.0
31	37.5881323983291	40.0	36.0	41.0	33.0	41.0
32	37.495088704862155	40.0	36.0	41.0	33.0	41.0
33	37.41253197207482	40.0	35.0	41.0	33.0	41.0
34	37.264409208918664	40.0	35.0	41.0	32.0	41.0
35	37.18589816751505	40.0	35.0	41.0	32.0	41.0
36	37.12068534672807	39.0	35.0	41.0	32.0	41.0
37	37.081164318143315	39.0	35.0	41.0	32.0	41.0
38	36.96163170812875	39.0	35.0	41.0	31.0	41.0
39	36.89254859935581	39.0	35.0	41.0	31.0	41.0
40	36.80656309055981	39.0	35.0	41.0	31.0	41.0
41	36.66845694267072	39.0	35.0	41.0	31.0	41.0
42	36.61584072890924	39.0	35.0	41.0	31.0	41.0
43	36.54805152054322	39.0	35.0	41.0	31.0	41.0
44	36.474865053516055	39.0	35.0	41.0	31.0	41.0
45	36.36878564383259	39.0	35.0	40.0	30.0	41.0
46	36.308067835462005	38.0	35.0	40.0	30.0	41.0
47	36.20888682516246	38.0	35.0	40.0	30.0	41.0
48	36.156966218026426	38.0	35.0	40.0	30.0	41.0
49	36.11412453881521	38.0	35.0	40.0	30.0	41.0
50	35.98773918235586	38.0	35.0	40.0	30.0	41.0
51	34.792282130358366	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	26.0
9	77.0
10	84.0
11	53.0
12	41.0
13	31.0
14	43.0
15	51.0
16	112.0
17	205.0
18	429.0
19	730.0
20	1246.0
21	1954.0
22	3130.0
23	4283.0
24	6092.0
25	8291.0
26	10878.0
27	13532.0
28	16533.0
29	20134.0
30	25559.0
31	32327.0
32	41787.0
33	58194.0
34	107528.0
35	131536.0
36	125869.0
37	188831.0
38	332958.0
39	532004.0
40	185.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.72985113537658	23.514567492464263	26.495884627814416	11.259696744344742
2	32.11984617362293	26.09888426619508	24.401856392672943	17.379413167509046
3	32.40968226755746	25.4430437535366	24.861209058023682	17.286064920882254
4	29.196195908775817	26.28576096841898	25.92840658027048	18.589636542534723
5	26.848313304107442	29.822602289615034	23.505256695664293	19.823827710613227
6	28.759970061283063	34.14635611455043	22.543901908653275	14.54977191551323
7	80.61149709202792	6.374231558915719	8.282764693939091	4.731506655117275
8	81.67989600741019	5.612428171707912	7.865100370389504	4.842575450492391
9	74.68262196843459	7.713664765662262	10.433018127821022	7.17069513808212
10	42.79770822245476	24.619969316419322	17.612843853732787	14.969478607393134
11	32.35093414323249	25.188889035725825	23.692193467544964	18.767983353496714
12	29.91168559061087	22.784601023346674	27.70232361446333	19.60138977157912
13	25.92828644095693	22.972739188362826	29.830831832593073	21.268142538087165
14	21.736025094699816	27.006476710393372	28.76609716627401	22.4914010286328
15	20.966052234170746	25.53170656693502	32.96893077212336	20.533310426770885
16	24.54554301167634	25.37402371790328	29.979324024138393	20.10110924628199
17	24.269222590515962	24.850997216372107	27.68916835962983	23.190611833482105
18	24.349896139563437	26.15679141532521	28.51242300571743	20.98088943939392
19	25.861729261251348	27.024017050171377	25.977964047109026	21.13628964146825
20	27.62020839365328	26.446567439602966	26.60713363215985	19.326090534583905
21	26.158833783655528	27.034349031136507	26.845910517836487	19.960906667371482
22	24.348454467800863	25.825206909932756	26.85365950356033	22.97267911870605
23	24.39651019322006	26.24671569151588	27.555213025023818	21.80156109024024
24	24.21870400916903	24.84505032035148	27.762933898148294	23.173311772331196
25	23.705468861692015	26.13768926447108	26.484351253713807	23.672490620123096
26	23.080984709869565	27.4247417305107	27.033988613195863	22.460284946423872
27	23.829332493959996	27.071832496963477	26.839843482502314	22.258991526574214
28	21.598285371717044	27.505895836812368	27.35968629222446	23.536132499246126
29	23.826028662837427	25.725431210031154	27.14175357744841	23.30678654968301
30	24.133164817922864	24.813453680888358	27.00869928769401	24.04468221349477
31	23.557937784655085	26.257408090421652	25.80820719706572	24.376446927857543
32	25.32692910699247	26.259630667722288	26.020433294448242	22.393006930836997
33	24.05255133853216	26.12056941229049	26.44530597681071	23.381573272366637
34	22.334378945825577	25.62349300248568	29.204065033813208	22.83806301787553
35	22.882754842515382	24.843308300305033	27.92824559359033	24.34569126358926
36	21.517431613699245	28.496985103926516	26.66347897021386	23.32210431216038
37	22.450613731683262	27.07813981092475	27.616183726649425	22.85506273074257
38	21.882054430317396	26.450532036950047	26.859366120953858	24.8080474117787
39	23.32979322822745	25.53230726350276	28.310168471359386	22.8277310369104
40	23.481709390208884	24.136228370418337	30.00461334964024	22.377448889732534
41	20.951815725515306	25.968412971681964	27.851957129487353	25.227814173315377
42	22.242592510274914	25.99376236684059	27.195215571977265	24.56842955090723
43	22.55525507378356	25.11842732832993	27.463967216384116	24.86235038150239
44	21.832977520733042	25.023697479597338	27.713796918907164	25.429528080762452
45	22.551110267466154	23.6812607900121	28.016307710421007	25.751321232100743
46	22.233161574161397	25.49236094174805	27.446306737292563	24.828170746797987
47	20.84375041297889	25.094159186993238	29.611217167427352	24.45087323260052
48	21.349296644388833	24.1370092759564	29.386436511779056	25.12725756787571
49	21.86871896651357	23.951033618584113	29.840322838363363	24.339924576538955
50	20.15751465399277	23.979566705551758	29.811969960366042	26.05094868008943
51	20.13270588574511	23.61091922192975	28.564503398140484	27.691871494184657
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	415.0
1	1228.0
2	2041.0
3	3483.0
4	4925.0
5	3529.5
6	2134.0
7	2153.5
8	2173.0
9	2204.5
10	2236.0
11	2361.0
12	2486.0
13	2546.5
14	2607.0
15	2795.0
16	2983.0
17	2878.5
18	2774.0
19	3090.0
20	3406.0
21	4510.0
22	5614.0
23	6112.0
24	6610.0
25	8007.5
26	11998.5
27	14592.0
28	18252.5
29	21913.0
30	25969.0
31	30025.0
32	32987.5
33	35950.0
34	39828.0
35	43706.0
36	48745.0
37	53784.0
38	57098.0
39	60412.0
40	67063.5
41	73715.0
42	79440.5
43	85166.0
44	94149.0
45	103132.0
46	118208.5
47	133285.0
48	140166.5
49	147048.0
50	146161.0
51	145274.0
52	129773.0
53	114272.0
54	105758.5
55	97245.0
56	91240.5
57	85236.0
58	83776.0
59	82316.0
60	81092.0
61	79868.0
62	74506.0
63	69144.0
64	58477.0
65	47810.0
66	39212.5
67	30615.0
68	25947.0
69	21279.0
70	17816.0
71	14353.0
72	11754.0
73	9155.0
74	8141.0
75	5598.5
76	4070.0
77	3273.0
78	2476.0
79	1752.5
80	1029.0
81	753.5
82	478.0
83	363.5
84	249.0
85	171.0
86	93.0
87	72.0
88	51.0
89	45.0
90	39.0
91	27.0
92	15.0
93	8.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1664734.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.8706570209977
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.91489585697089	16.689144996605254
2	11.764207090500761	5.616387051626658
3	4.6446260772619965	3.3261082824330925
4	2.3534381102649213	2.2471245580111563
5	1.3661092613286134	1.6304962815191923
6	0.9457597409994939	1.354554384099992
7	0.6831717054802943	1.1415430227579113
8	0.5024295137224103	0.9594658079435452
9	0.4275698452461601	0.9185735815552938
>10	5.622122837866785	32.809306376654554
>50	1.6233002424622964	26.400082115005453
>100	0.1457560075430407	4.997428608082714
>500	0.004324349076608625	0.7373304861370602
>1k	0.0022893612758516247	1.172454447568187
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC	3405	0.2045371813154534	No Hit
GCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC	2804	0.16843531759428232	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTC	2654	0.15942486907818304	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2314	0.1390011857750247	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGC	2027	0.12176119428088812	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCG	1796	0.10788510356609525	No Hit
CTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGCT	1680	0.10091702338031182	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.80557254191961E-4	0.0	0.0	0.10788510356609525	0.0
2	4.80557254191961E-4	0.0	0.0	0.6703773695977856	0.0
3	4.80557254191961E-4	0.0	0.0	1.0539221280997444	0.0
4	4.80557254191961E-4	0.0	0.0	1.4945330605369986	0.0
5	4.80557254191961E-4	0.0	0.0	2.747165613245119	0.0
6	4.80557254191961E-4	0.0	0.0	3.510590881185823	0.0
7	4.80557254191961E-4	0.0	0.0	4.277500189219419	0.0
8	4.80557254191961E-4	0.0	0.0	5.438646654660745	0.0
9	4.80557254191961E-4	0.0	0.0	5.916981331552067	0.0
10	4.80557254191961E-4	0.0	0.0	6.921165783842945	0.0
11	4.80557254191961E-4	0.0	0.0	8.21885057913156	0.0
12	4.80557254191961E-4	0.0	0.0	9.306892272278935	0.0
13	4.80557254191961E-4	0.0	0.0	9.726478824845291	0.0
14	4.80557254191961E-4	0.0	0.0	9.913715945009834	0.0
15	4.80557254191961E-4	0.0	0.0	10.256593545875798	0.0
16	4.80557254191961E-4	0.0	0.0	10.919161860092963	0.0
17	4.80557254191961E-4	0.0	0.0	11.634170984673828	0.0
18	4.80557254191961E-4	0.0	0.0	12.387985107530692	0.0
19	4.80557254191961E-4	0.0	0.0	12.901700812261899	0.0
20	5.406269109659561E-4	0.0	0.0	13.404183491176369	0.0
21	5.406269109659561E-4	0.0	0.0	14.01467141297048	0.0
22	5.406269109659561E-4	0.0	0.0	14.639936470330996	0.0
23	5.406269109659561E-4	0.0	0.0	15.23000070882195	0.0
24	6.006965677399513E-4	0.0	0.0	15.729660113868041	0.0
25	6.006965677399513E-4	0.0	0.0	16.193397864163284	0.0
26	6.006965677399513E-4	0.0	0.0	16.62181465627542	0.0
27	6.006965677399513E-4	0.0	0.0	17.036535566643078	0.0
28	6.006965677399513E-4	0.0	0.0	17.447952645888172	0.0
29	6.607662245139464E-4	0.0	0.0	17.91042893339116	0.0
30	6.607662245139464E-4	0.0	0.0	18.443847485544236	0.0
31	8.409751948359318E-4	0.0	0.0	18.93149295923553	0.0
32	8.409751948359318E-4	0.0	0.0	19.40676408363138	0.0
33	9.010448516099268E-4	0.0	0.0	19.863834102024708	0.0
34	9.010448516099268E-4	0.0	0.0	20.320303423850298	0.0
35	9.010448516099268E-4	0.0	0.0	20.785122427967472	0.0
36	9.010448516099268E-4	0.0	0.0	21.269524140192967	0.0
37	9.010448516099268E-4	0.0	0.0	21.73986955273335	0.0
38	9.61114508383922E-4	0.0	0.0	22.23033830029302	0.0
39	0.0010812538219319122	0.0	0.0	22.722549067899134	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTATCG	20	7.0342E-4	45.0	41
CCTACGC	20	7.0342E-4	45.0	24
ACGATTG	30	2.1660908E-6	44.999996	1
ATACCGG	65	0.0	41.538464	2
CGTAAGG	150	0.0	40.5	2
ACCGGAC	45	1.9292202E-8	40.0	17
ACGTACG	40	3.4594632E-7	39.375	1
AGTTACG	100	0.0	38.250004	1
AGGTACG	65	9.094947E-12	38.076927	1
TCGTTAG	90	0.0	37.5	1
ACGATAG	60	1.5643309E-10	37.499996	1
ACCGTTA	30	1.14014205E-4	37.499996	9
GCTAACG	60	1.5643309E-10	37.499996	1
TGTAGCG	175	0.0	37.285713	1
CGAACGT	55	2.750312E-9	36.81818	19
GTTACGG	210	0.0	36.42857	2
TACGGGA	415	0.0	36.3253	4
GGTACGA	25	0.002107379	36.000004	9
TAGCGTA	25	0.002107379	36.000004	1
CGCATAT	25	0.002107379	36.000004	36
>>END_MODULE