FastQCFastQC Report
Sat 18 Jun 2016
SRR3550413_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550413_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1625517
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGC34550.21254776172749962No Hit
CCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGC25990.15988759268589625No Hit
CTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGCT22660.13940180262648746Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC253.8915212E-545.0000048
TAACGCG650.045.0000041
TATGCGT253.8915212E-545.00000445
TAATGCG253.8915212E-545.0000041
TACGCTA406.8175723E-945.035
GTATACG453.8562575E-1045.01
ATCGCCT207.0341496E-445.015
ACGTTAG501.0822987E-940.5000041
ATGACGG1450.040.3448262
ACGTACG451.9292202E-840.01
CGAATAT1700.039.70588314
ATCGAAC1100.038.86363610
TACGGGT1450.038.7931024
CGAACCC700.038.57142612
CGACTTA356.2498875E-638.57142640
TACGAGT301.1401301E-437.49999642
TCGTAGC301.1401301E-437.49999638
CGACTAT301.1401301E-437.49999626
ACGCTAG552.750312E-936.818181
ACGCATG800.036.56251