##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550413_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1625517
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33082951454829	33.0	31.0	34.0	30.0	34.0
2	32.486188086621056	34.0	31.0	34.0	31.0	34.0
3	32.49616275929443	34.0	31.0	34.0	31.0	34.0
4	36.00818139705706	37.0	35.0	37.0	35.0	37.0
5	36.04077656524048	37.0	35.0	37.0	35.0	37.0
6	36.051283991493165	37.0	35.0	37.0	35.0	37.0
7	36.12225156673231	37.0	35.0	37.0	35.0	37.0
8	36.107264334977735	37.0	36.0	37.0	35.0	37.0
9	37.5926471393409	39.0	38.0	39.0	35.0	39.0
10	37.46315049304314	39.0	37.0	39.0	35.0	39.0
11	37.30837265928317	39.0	37.0	39.0	34.0	39.0
12	37.17767024275969	39.0	37.0	39.0	34.0	39.0
13	37.148769283864766	39.0	37.0	39.0	34.0	39.0
14	38.357197740780315	40.0	38.0	41.0	34.0	41.0
15	38.43897418482858	40.0	38.0	41.0	34.0	41.0
16	38.4839100421589	40.0	38.0	41.0	34.0	41.0
17	38.46378167684497	40.0	38.0	41.0	34.0	41.0
18	38.40021420877173	40.0	37.0	41.0	34.0	41.0
19	38.371543330521924	40.0	37.0	41.0	34.0	41.0
20	38.2829838137651	40.0	37.0	41.0	34.0	41.0
21	38.19512253639919	40.0	37.0	41.0	34.0	41.0
22	38.21425552608801	40.0	37.0	41.0	34.0	41.0
23	38.2191038297354	40.0	37.0	41.0	34.0	41.0
24	38.22475187894067	40.0	37.0	41.0	34.0	41.0
25	38.15427645481407	40.0	37.0	41.0	34.0	41.0
26	38.10547044417253	40.0	37.0	41.0	34.0	41.0
27	38.060999669643564	40.0	37.0	41.0	34.0	41.0
28	38.00560252522736	40.0	37.0	41.0	34.0	41.0
29	37.99252176384498	40.0	37.0	41.0	34.0	41.0
30	37.932591907682294	40.0	36.0	41.0	34.0	41.0
31	37.89113309796206	40.0	36.0	41.0	33.0	41.0
32	37.83719825753899	40.0	36.0	41.0	33.0	41.0
33	37.770691416946114	40.0	36.0	41.0	33.0	41.0
34	37.653712019006875	40.0	36.0	41.0	33.0	41.0
35	37.59543886652677	40.0	36.0	41.0	33.0	41.0
36	37.52399144395291	40.0	36.0	41.0	33.0	41.0
37	37.49004901209892	40.0	36.0	41.0	33.0	41.0
38	37.387476722790346	40.0	35.0	41.0	33.0	41.0
39	37.341890610802594	40.0	35.0	41.0	33.0	41.0
40	37.26239897829429	39.0	35.0	41.0	32.0	41.0
41	37.14443650850775	39.0	35.0	41.0	32.0	41.0
42	37.12356253425833	39.0	35.0	41.0	32.0	41.0
43	37.07741229405783	39.0	35.0	41.0	32.0	41.0
44	37.01390634487366	39.0	35.0	41.0	32.0	41.0
45	36.928964138793994	39.0	35.0	41.0	32.0	41.0
46	36.87063192818039	39.0	35.0	41.0	31.0	41.0
47	36.8005182351215	39.0	35.0	41.0	31.0	41.0
48	36.77140072973706	39.0	35.0	41.0	31.0	41.0
49	36.74489962270465	39.0	35.0	41.0	31.0	41.0
50	36.64246882684094	39.0	35.0	41.0	31.0	41.0
51	35.496862229063126	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	21.0
9	44.0
10	68.0
11	55.0
12	29.0
13	26.0
14	39.0
15	49.0
16	87.0
17	136.0
18	310.0
19	496.0
20	865.0
21	1397.0
22	2109.0
23	3113.0
24	4521.0
25	6279.0
26	8274.0
27	10539.0
28	12751.0
29	16016.0
30	20607.0
31	26995.0
32	36064.0
33	51532.0
34	100182.0
35	126515.0
36	112321.0
37	171337.0
38	322054.0
39	590442.0
40	241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.444537337966935	22.322436492512843	25.993084046491056	10.239942123029165
2	31.0963834890684	24.38565699405174	26.31507391186927	18.20288560501059
3	32.632141035744326	24.437948049758937	26.311075184079897	16.618835730416844
4	28.687488349860384	26.634233908350392	25.550148045206537	19.12812969658269
5	26.759301809824198	30.48949964841955	23.716085405443316	19.03511313631294
6	28.626154017460294	34.166852761306096	22.451933753999498	14.755059467234116
7	83.2238604702381	5.852907105862319	6.498793922179836	4.424438501719761
8	85.15370802027908	4.502075339722685	6.163515976763085	4.180700663235143
9	78.09583043425569	7.296755432271702	8.659214268445055	5.948199865027557
10	44.57640246149379	23.100527401435976	17.190284690962937	15.132785446107299
11	31.790685671081874	26.01953716878999	23.529990766014752	18.659786394113382
12	30.219370206525063	23.664532576404923	26.78329417656044	19.33280304050957
13	25.25824091658223	24.96737960907207	28.69548580543913	21.07889366890657
14	20.902519013950638	27.99337072451411	28.951527421737204	22.152582839798047
15	20.64327841542106	26.87766415238967	32.162874949939	20.316182482250262
16	22.90791175976628	27.043949709538566	29.811746047565173	20.236392483129983
17	23.09148412474308	26.237683149422615	28.27814166200661	22.392691063827694
18	23.140022528217177	26.798673898827264	29.098434528829902	20.962869044125654
19	24.415739730805644	28.420619409086463	26.35216980197685	20.811471058131044
20	26.467702275645227	27.441976921803956	27.153883964301816	18.936436838248998
21	25.211671117558293	27.110574666398445	27.577749109975475	20.10000510606779
22	23.648599184136494	26.26456690394502	27.2338585200893	22.852975391829183
23	23.79685970678867	26.237190998310076	27.98358922115241	21.982360073748843
24	23.34746422215209	25.19376912083971	28.947897807282235	22.510868849725966
25	22.73215229370102	27.648557351291924	26.793690868812813	22.82559948619424
26	21.518815244626786	28.722369559961535	27.570120767731126	22.18869442768055
27	22.630277013405582	28.4735871725734	27.505772009766737	21.390363804254278
28	20.63312779872496	28.45642340252363	27.977744926691017	22.932703872060397
29	22.0138577449513	25.955926637494407	28.72938271331521	23.300832904239083
30	22.910249477550835	26.586925882657642	28.31043907876694	22.192385561024587
31	22.73036824591807	27.239210663438158	27.35861882711777	22.671802263526004
32	24.01598998964637	27.189134287737378	26.763423575391705	22.031452147224545
33	23.463365809154872	26.68289534960262	27.274645543540917	22.579093297701593
34	21.291133836188735	26.20840015822658	29.379268257422098	23.121197748162583
35	22.561191300983012	25.82882861268138	28.879673359306608	22.730306727029
36	22.027514938324238	27.87383952305636	28.61501909853911	21.483626440080293
37	22.54162829425961	27.30497435585109	28.44498088915711	21.708416460732185
38	22.162548899826948	26.93456912477692	27.20617502000902	23.696706955387118
39	22.298751720221937	25.735750533522566	29.445216506502238	22.52028123975326
40	23.187576629466193	24.72019671279968	30.64452724886913	21.447699408864995
41	21.542315460250492	26.23165429829402	29.57182238020273	22.654207861252758
42	22.364884525969273	25.463898562734194	28.646885883075967	23.524331028220562
43	22.190970626576036	25.074668551605427	29.20658473581021	23.527776086008327
44	21.4418551144036	26.114276257953623	27.562246349930515	24.88162227771226
45	21.907860699088353	25.085618913859403	28.09512296703141	24.91139742002083
46	21.95566087589364	26.16306073698399	28.553500209471817	23.327778177650558
47	21.207898779280683	25.465744129406215	30.428288353797594	22.898068737515512
48	21.472306964491914	23.981293336212417	30.29128578784473	24.25511391145094
49	21.410849594313685	24.62902571920195	31.0115489410446	22.948575745439758
50	20.503015348347635	24.56061671455912	30.122170361798737	24.814197575294507
51	20.081549439347604	24.321062160531078	29.401907208598864	26.19548119152245
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	254.0
1	792.5
2	1331.0
3	2168.0
4	3005.0
5	2244.0
6	1483.0
7	1457.0
8	1431.0
9	1482.0
10	1533.0
11	1549.5
12	1566.0
13	1716.0
14	1866.0
15	1866.0
16	1866.0
17	2230.0
18	2594.0
19	2899.0
20	3204.0
21	3878.0
22	4552.0
23	5590.0
24	6628.0
25	8309.0
26	12985.5
27	15981.0
28	18938.5
29	21896.0
30	25331.0
31	28766.0
32	36068.5
33	43371.0
34	47903.5
35	52436.0
36	55012.5
37	57589.0
38	64811.5
39	72034.0
40	77138.5
41	82243.0
42	89480.5
43	96718.0
44	105008.5
45	113299.0
46	122495.0
47	131691.0
48	141349.0
49	151007.0
50	147633.5
51	144260.0
52	127064.5
53	109869.0
54	98349.0
55	86829.0
56	81300.0
57	75771.0
58	74572.0
59	73373.0
60	69832.0
61	66291.0
62	61651.5
63	57012.0
64	46884.0
65	36756.0
66	30206.5
67	23657.0
68	19728.0
69	15799.0
70	12718.5
71	9638.0
72	8562.5
73	7487.0
74	6132.0
75	3605.0
76	2433.0
77	2112.0
78	1791.0
79	1225.5
80	660.0
81	582.5
82	505.0
83	349.0
84	193.0
85	114.5
86	36.0
87	25.0
88	14.0
89	11.5
90	9.0
91	9.0
92	9.0
93	9.5
94	10.0
95	6.0
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1625517.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.833724832793376
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.64644251944549	15.206412615525334
2	11.854308255826473	5.1764740908165185
3	4.501557777111718	2.948573214731348
4	2.2798779312779374	1.9911290961552273
5	1.3377257868738999	1.4603768366168361
6	0.8666144800217764	1.1352853255745867
7	0.6112082874104265	0.9341467493989498
8	0.47376962332655603	0.8275324471878551
9	0.3700340969887017	0.727130038716224
>10	5.784829642475247	32.179477318819174
>50	2.1157512349501655	31.70232457156907
>100	0.15332064259418451	4.5006024975240795
>500	0.0022798608487079506	0.3216271619212878
>1k	0.0022798608487079506	0.8889080354435128
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGC	3455	0.21254776172749962	No Hit
CCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGC	2599	0.15988759268589625	No Hit
CTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGCT	2266	0.13940180262648746	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.84556667201881E-4	0.0	0.0	0.14795292820684128	0.0
2	1.84556667201881E-4	0.0	0.0	0.7050064687111854	0.0
3	1.84556667201881E-4	0.0	0.0	0.9971596728917631	0.0
4	1.84556667201881E-4	0.0	0.0	1.331084202749033	0.0
5	1.84556667201881E-4	0.0	0.0	2.2851806532936907	0.0
6	1.84556667201881E-4	0.0	0.0	2.8714556661049992	0.0
7	1.84556667201881E-4	0.0	0.0	3.4585304244741826	0.0
8	1.84556667201881E-4	0.0	0.0	4.40672106166838	0.0
9	1.84556667201881E-4	0.0	0.0	4.7413222993053905	0.0
10	1.84556667201881E-4	0.0	0.0	5.486992753690057	0.0
11	1.84556667201881E-4	0.0	0.0	6.613342093623136	0.0
12	1.84556667201881E-4	0.0	0.0	7.4338195171136325	0.0
13	1.84556667201881E-4	0.0	0.0	7.757408873607597	0.0
14	1.84556667201881E-4	0.0	0.0	7.895703336230873	0.0
15	1.84556667201881E-4	0.0	0.0	8.172046185921156	0.0
16	1.84556667201881E-4	0.0	0.0	8.774008515444624	0.0
17	1.84556667201881E-4	0.0	0.0	9.455699325199307	0.0
18	1.84556667201881E-4	0.0	0.0	10.173809317281824	0.0
19	1.84556667201881E-4	0.0	0.0	10.642460214196468	0.0
20	1.84556667201881E-4	0.0	0.0	11.095792907733355	0.0
21	1.84556667201881E-4	0.0	0.0	11.688219809451393	0.0
22	1.84556667201881E-4	0.0	0.0	12.31614311016126	0.0
23	1.84556667201881E-4	0.0	0.0	12.932746935282744	0.0
24	1.84556667201881E-4	0.0	0.0	13.443107638985012	0.0
25	1.84556667201881E-4	0.0	0.0	13.898101342526717	0.0
26	2.4607555626917466E-4	0.0	0.0	14.300865509250288	0.0
27	2.4607555626917466E-4	0.0	0.0	14.709904602658723	0.0
28	3.0759444533646834E-4	0.0	0.0	15.103502454911268	0.0
29	3.0759444533646834E-4	0.0	0.0	15.533642527269786	0.0
30	3.0759444533646834E-4	0.0	0.0	16.02634730980974	0.0
31	3.0759444533646834E-4	0.0	0.0	16.46983698109586	0.0
32	3.69113334403762E-4	0.0	0.0	16.92360030685622	0.0
33	3.69113334403762E-4	0.0	0.0	17.35017228364883	0.0
34	3.69113334403762E-4	0.0	0.0	17.784926272687397	0.0
35	3.69113334403762E-4	0.0	0.0	18.23629036177413	0.0
36	3.69113334403762E-4	0.0	0.0	18.69503671754894	0.0
37	3.69113334403762E-4	0.0	0.0	19.141294738843087	0.0
38	3.69113334403762E-4	0.0	0.0	19.598749197947484	0.0
39	3.69113334403762E-4	0.0	0.0	20.063955037074358	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGATC	25	3.8915212E-5	45.000004	8
TAACGCG	65	0.0	45.000004	1
TATGCGT	25	3.8915212E-5	45.000004	45
TAATGCG	25	3.8915212E-5	45.000004	1
TACGCTA	40	6.8175723E-9	45.0	35
GTATACG	45	3.8562575E-10	45.0	1
ATCGCCT	20	7.0341496E-4	45.0	15
ACGTTAG	50	1.0822987E-9	40.500004	1
ATGACGG	145	0.0	40.344826	2
ACGTACG	45	1.9292202E-8	40.0	1
CGAATAT	170	0.0	39.705883	14
ATCGAAC	110	0.0	38.863636	10
TACGGGT	145	0.0	38.793102	4
CGAACCC	70	0.0	38.571426	12
CGACTTA	35	6.2498875E-6	38.571426	40
TACGAGT	30	1.1401301E-4	37.499996	42
TCGTAGC	30	1.1401301E-4	37.499996	38
CGACTAT	30	1.1401301E-4	37.499996	26
ACGCTAG	55	2.750312E-9	36.81818	1
ACGCATG	80	0.0	36.5625	1
>>END_MODULE