FastQCFastQC Report
Sat 18 Jun 2016
SRR3550410_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550410_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101735
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC32070.2910863320126891No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC18560.16846156289851916No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT14920.13542276500247336Illumina Single End Adapter 1 (95% over 21bp)
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT13000.11799570677159209No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT11340.10292856267614262No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11280.1023839671064276No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAA207.033158E-445.00000442
CATGCGA253.890699E-545.00000418
ACGCAAT253.890699E-545.00000431
TTTCGTC207.033158E-445.00000439
TTCGTGC253.890699E-545.00000426
CGATTAT207.033158E-445.00000410
TCTAGCG207.033158E-445.0000041
CGTTAAT253.890699E-545.00000418
TAGCACG950.045.0000041
TCTCGAT207.033158E-445.00000423
TTACGTC207.033158E-445.00000416
CCGCGTA207.033158E-445.00000445
GTTATAC207.033158E-445.00000428
ATTTCGA207.033158E-445.00000426
GACCGAC351.2118653E-745.09
CACGCAA351.2118653E-745.036
ACTAGTC351.2118653E-745.018
TCTCGAC351.2118653E-745.043
TAATACG302.1654196E-644.9999961
ATCTCCG302.1654196E-644.99999630