FastQCFastQC Report
Sat 18 Jun 2016
SRR3550409_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550409_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3000538
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195260.6507499655061859No Hit
CGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG51950.17313561767922953No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC41730.13907505920604907No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT35010.11667907555245093No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCT30170.1005486349447999No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCGC207.035104E-445.00000427
CCGATCA502.1827873E-1145.011
CGTTTTT131600.042.4183121
CGTAAGG5550.039.729732
GTCGATA403.4607E-739.37500411
ATTAGCG1400.038.5714261
ACGCGAT301.1403602E-437.50000435
GCGCTAG2200.036.818181
CGTTCGG6400.036.2109445
ATAGCGC250.002107648436.012
TACGGGT3250.036.04
GTACGAG2250.036.01
CGCACGG3350.035.5970152
ACCGCGT950.035.52631825
CTAAACG702.1827873E-1135.3571431
CACGTGA19050.035.31495743
CGAAAGG3450.035.2173922
AGGGCGA22150.035.146736
GCGAGAC20550.035.03649521
ACGTGCG1350.035.01
Produced by FastQC (version 0.11.5)