##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550409_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3000538
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31362708954194	33.0	31.0	34.0	30.0	34.0
2	32.500747865882715	34.0	31.0	34.0	31.0	34.0
3	32.48008123876451	34.0	31.0	34.0	30.0	34.0
4	35.99578575575447	37.0	35.0	37.0	35.0	37.0
5	36.02473923009807	37.0	35.0	37.0	35.0	37.0
6	36.06999411438882	37.0	35.0	37.0	35.0	37.0
7	36.18628792569866	37.0	36.0	37.0	35.0	37.0
8	36.105879012363786	37.0	37.0	37.0	35.0	37.0
9	37.630268305217264	39.0	38.0	39.0	35.0	39.0
10	37.4846200914636	39.0	37.0	39.0	35.0	39.0
11	37.42661216088582	39.0	37.0	39.0	35.0	39.0
12	37.36073197539908	39.0	37.0	39.0	35.0	39.0
13	37.376923071795794	39.0	37.0	39.0	35.0	39.0
14	38.67593511563593	40.0	38.0	41.0	35.0	41.0
15	38.7221651583816	40.0	38.0	41.0	35.0	41.0
16	38.750163803957825	40.0	38.0	41.0	35.0	41.0
17	38.716065585571656	40.0	38.0	41.0	35.0	41.0
18	38.61470509621941	40.0	38.0	41.0	35.0	41.0
19	38.496690260213335	40.0	37.0	41.0	34.0	41.0
20	38.40080112299861	40.0	37.0	41.0	34.0	41.0
21	38.337814085340696	40.0	37.0	41.0	34.0	41.0
22	38.31321649650829	40.0	37.0	41.0	34.0	41.0
23	38.2505227395887	40.0	37.0	41.0	34.0	41.0
24	38.210106654206676	40.0	37.0	41.0	34.0	41.0
25	38.14840971852381	40.0	37.0	41.0	34.0	41.0
26	38.13757432833712	40.0	37.0	41.0	34.0	41.0
27	38.11215422034315	40.0	36.0	41.0	34.0	41.0
28	38.023119853839546	40.0	36.0	41.0	34.0	41.0
29	37.995154868893515	40.0	36.0	41.0	34.0	41.0
30	37.903817582046955	40.0	36.0	41.0	34.0	41.0
31	37.78442232692937	40.0	36.0	41.0	33.0	41.0
32	37.61651543823141	40.0	36.0	41.0	33.0	41.0
33	37.46089034699777	40.0	36.0	41.0	33.0	41.0
34	37.25861962088132	40.0	36.0	41.0	32.0	41.0
35	37.13222662069269	40.0	36.0	41.0	32.0	41.0
36	37.0460310784266	40.0	35.0	41.0	32.0	41.0
37	37.00199464229415	40.0	35.0	41.0	32.0	41.0
38	36.896349254700326	40.0	35.0	41.0	31.0	41.0
39	36.812986204474	40.0	35.0	41.0	31.0	41.0
40	36.74451148427382	39.0	35.0	41.0	31.0	41.0
41	36.65849457663926	39.0	35.0	41.0	31.0	41.0
42	36.58288780212082	39.0	35.0	41.0	31.0	41.0
43	36.49997400466183	39.0	35.0	41.0	31.0	41.0
44	36.373816962158116	39.0	35.0	41.0	30.0	41.0
45	36.2536058533503	39.0	35.0	41.0	30.0	41.0
46	36.15693318998127	39.0	35.0	41.0	30.0	41.0
47	36.102829225958814	39.0	35.0	41.0	30.0	41.0
48	36.060176874947096	39.0	35.0	41.0	30.0	41.0
49	35.998857204941245	39.0	35.0	41.0	30.0	41.0
50	35.914759619774856	38.0	35.0	41.0	29.0	41.0
51	34.90860738974144	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	4.0
9	17.0
10	24.0
11	22.0
12	33.0
13	26.0
14	33.0
15	60.0
16	98.0
17	224.0
18	460.0
19	967.0
20	1781.0
21	2877.0
22	4728.0
23	7592.0
24	12749.0
25	21045.0
26	30462.0
27	34740.0
28	35605.0
29	37080.0
30	41215.0
31	50078.0
32	63031.0
33	86061.0
34	156123.0
35	217882.0
36	206690.0
37	305992.0
38	572477.0
39	1109424.0
40	935.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.83809703459846	20.659595045955093	24.240719497636757	13.261588421809689
2	34.43499132488907	22.611644978333885	24.927929591293292	18.02543410548375
3	30.813074188695495	23.95900335206553	28.593938820304892	16.633983638934087
4	26.389467488830338	25.688526524243322	28.853059018082757	19.068946968843587
5	24.277046316360597	29.609656668237495	27.06544626330345	19.04785075209846
6	25.158254952945104	33.66852877717263	25.835200220760413	15.338016049121858
7	81.46279100614623	4.9140854073502815	9.375085401351358	4.248038185152129
8	82.71546635969949	4.13922436576374	9.303664876098887	3.84164439843788
9	76.50834616992019	6.487536568442059	11.487939829457252	5.516177432180496
10	38.0920354949679	29.556666171199964	18.813759399147752	13.53753893468438
11	28.36794601501464	25.091566912333725	27.496402311852076	19.044084760799564
12	24.859941783773444	23.525747715909613	30.770981737275115	20.84332876304183
13	24.157901016417725	25.142224494407333	31.256594650692644	19.4432798384823
14	21.26288685562389	26.541140288841536	31.057163748634416	21.138809106900165
15	20.530951449373415	25.37684908506408	32.99938211080813	21.09281735475438
16	23.720379478613502	25.53385426213566	31.046065738877495	19.699700520373348
17	22.557754642667415	25.24087347002438	30.28943476136613	21.911937125942078
18	24.633515722847036	23.90218020901585	30.49703086579807	20.967273202339047
19	23.09425842965495	27.18909075639102	28.57944142017198	21.137209393782047
20	25.26270288861531	25.941314524261983	28.56407750876676	20.23190507835595
21	23.050599592473084	27.61298140533464	29.516340069680837	19.820078932511436
22	23.257595804485728	24.135171759197853	29.372399216407192	23.23483321990923
23	21.94629763062491	27.0672126132047	29.472747887212225	21.513741868958167
24	21.78442665948573	24.882571058923432	31.440361695136005	21.892640586454828
25	21.99505555337076	26.86314920857526	28.592505743969916	22.54928949408406
26	21.764996810571972	27.026419928692786	29.144606733859064	22.063976526876182
27	21.945797720275497	26.247926205233863	28.647962465397868	23.15831360909277
28	20.01321096416709	27.989980463503546	28.918713910638694	23.07809466169067
29	21.65454995070884	26.284353006027583	28.398140600119042	23.662956443144527
30	22.660969466142404	25.283665795933928	29.44445296143558	22.61091177648808
31	23.348546160721845	27.172627042217094	26.95010028201609	22.52872651504497
32	22.586716115576607	26.927004423873317	27.58721935866168	22.899060101888395
33	21.801923521715104	27.028986135153094	26.721807889118555	24.447282454013248
34	21.0443927055748	28.070865958038194	28.11752425731652	22.767217079070488
35	21.14967382516069	28.83406242480515	27.13200099448832	22.88426275554584
36	20.943544124420356	31.387471180168358	25.105231128550948	22.563753566860342
37	22.17808939596832	29.86714382554062	26.212799171348607	21.741967607142453
38	21.587761928027575	29.81768602830559	26.04319625347188	22.551355790194958
39	23.359110932772722	26.899842628222004	25.78410938305064	23.956937055954633
40	23.65835726792995	26.22313065190309	27.44231201204584	22.676200068121116
41	20.36728080097636	28.670925014114136	26.557770639798594	24.40402354511091
42	21.755765132786188	28.44269927592985	26.54550617255972	23.256029418724243
43	21.66941395176465	26.79336172379753	27.662139256360025	23.87508506807779
44	22.335261209823038	27.070545348867437	26.385034950398893	24.209158490910628
45	22.52629361801117	25.292230926587166	27.09247474952825	25.089000705873417
46	22.43934254457034	26.44329116978355	26.06935822842437	25.048008057221736
47	20.64486435432579	27.482171530572185	28.58510707079864	23.28785704430339
48	20.994868253626517	26.942401662635167	28.821198065146987	23.241532018591332
49	22.44710781866452	25.95421221127678	27.953020425003782	23.64565954505492
50	20.7031872284237	25.597176239727677	28.85719161030455	24.842444921544068
51	20.56644508418157	24.843211450746498	27.263777362592972	27.326566102478957
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	830.0
1	1678.5
2	2527.0
3	17576.0
4	32625.0
5	22122.0
6	11619.0
7	11384.5
8	11150.0
9	11210.5
10	11271.0
11	11345.5
12	11420.0
13	11354.0
14	11288.0
15	10839.5
16	10391.0
17	10175.0
18	9959.0
19	9764.5
20	9570.0
21	10485.5
22	11401.0
23	12246.5
24	13092.0
25	15516.5
26	21694.0
27	25447.0
28	28181.5
29	30916.0
30	35774.0
31	40632.0
32	48668.5
33	56705.0
34	64390.0
35	72075.0
36	82854.0
37	93633.0
38	102703.0
39	111773.0
40	122126.5
41	132480.0
42	145312.5
43	158145.0
44	176675.5
45	195206.0
46	219294.5
47	243383.0
48	264988.5
49	286594.0
50	288242.5
51	289891.0
52	265151.5
53	240412.0
54	215869.5
55	191327.0
56	175908.5
57	160490.0
58	146944.5
59	133399.0
60	120925.5
61	108452.0
62	97467.0
63	86482.0
64	73886.0
65	61290.0
66	50764.0
67	40238.0
68	32626.0
69	25014.0
70	21132.0
71	17250.0
72	14535.5
73	11821.0
74	10735.0
75	8524.5
76	7400.0
77	5036.5
78	2673.0
79	2039.0
80	1405.0
81	971.0
82	537.0
83	478.0
84	419.0
85	332.5
86	246.0
87	130.5
88	15.0
89	14.0
90	13.0
91	13.5
92	14.0
93	20.0
94	26.0
95	14.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3000538.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.564636042691287
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.57691149179024	16.866838683639642
2	11.55948244491259	5.447899933124888
3	4.618059108930604	3.2646864637675472
4	2.327756754831144	2.194109628940483
5	1.4348655327843538	1.6906042025132808
6	0.9494075475315635	1.3423465988259253
7	0.6694255163230259	1.1042338054889962
8	0.47178610386233444	0.8893974262012208
9	0.4030436240057303	0.8547818678120113
>10	3.957632800610621	21.78736043801647
>50	1.5301452452271607	26.080396856870347
>100	0.4928843858474894	15.385488523955237
>500	0.005016341950177116	0.8087472254323498
>1k	0.0032964532815449622	1.4481864311080652
>5k	1.433240557193462E-4	0.17545484991735813
>10k+	1.433240557193462E-4	0.659467064386205
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19526	0.6507499655061859	No Hit
CGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG	5195	0.17313561767922953	No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	4173	0.13907505920604907	No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT	3501	0.11667907555245093	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCT	3017	0.1005486349447999	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.332735662737816E-4	0.0	0.0	0.12317791009478966	0.0
2	3.332735662737816E-4	0.0	0.0	0.46514991644831694	0.0
3	3.332735662737816E-4	0.0	0.0	0.8184532240551528	0.0
4	3.332735662737816E-4	0.0	0.0	1.141561946557584	0.0
5	3.332735662737816E-4	0.0	0.0	2.054398244581472	0.0
6	3.332735662737816E-4	0.0	0.0	2.8951141428637133	0.0
7	3.332735662737816E-4	0.0	0.0	3.6290158631552076	0.0
8	3.332735662737816E-4	0.0	0.0	4.748748391121858	0.0
9	3.332735662737816E-4	0.0	0.0	5.263489414231715	0.0
10	3.332735662737816E-4	0.0	0.0	6.244913412194746	0.0
11	3.666009229011597E-4	0.0	0.0	7.519151565485923	0.0
12	3.999282795285379E-4	0.0	0.0	8.450617855864515	0.0
13	3.999282795285379E-4	0.0	0.0	8.880240810148047	0.0
14	4.3325563615591604E-4	0.0	0.0	9.068740339232498	0.0
15	4.665829927832942E-4	0.0	0.0	9.355888843933988	0.0
16	4.999103494106724E-4	0.0	0.0	9.919587753929463	0.0
17	4.999103494106724E-4	0.0	0.0	10.637792289249461	0.0
18	4.999103494106724E-4	0.0	0.0	11.368927838940884	0.0
19	4.999103494106724E-4	0.0	0.0	11.838943549456797	0.0
20	5.332377060380505E-4	0.0	0.0	12.30046078403273	0.0
21	5.332377060380505E-4	0.0	0.0	12.882056484537106	0.0
22	5.665650626654286E-4	0.0	0.0	13.517775812204345	0.0
23	5.665650626654286E-4	0.0	0.0	14.19992014765352	0.0
24	5.665650626654286E-4	0.0	0.0	14.732491306559023	0.0
25	5.665650626654286E-4	0.0	0.0	15.209039178973905	0.0
26	5.665650626654286E-4	0.0	0.0	15.660691515988132	0.0
27	5.665650626654286E-4	0.0	0.0	16.110377538961345	0.0
28	5.665650626654286E-4	0.0	0.0	16.58229290880502	0.0
29	5.665650626654286E-4	0.0	0.0	17.09086837093881	0.0
30	5.998924192928068E-4	0.0	0.0	17.694660090957022	0.0
31	6.665471325475632E-4	0.0	0.0	18.205835086907747	0.0
32	6.998744891749413E-4	0.0	0.0	18.71721004699824	0.0
33	7.332018458023194E-4	0.0	0.0	19.205522476302583	0.0
34	7.332018458023194E-4	0.0	0.0	19.67120563045694	0.0
35	7.332018458023194E-4	0.0	0.0	20.186279927133068	0.0
36	7.332018458023194E-4	0.0	0.0	20.683957343649706	0.0
37	7.332018458023194E-4	0.0	0.0	21.190399854959345	0.0
38	7.332018458023194E-4	0.0	0.0	21.7262037674577	0.0
39	7.332018458023194E-4	0.0	0.0	22.2681399135755	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATCGC	20	7.035104E-4	45.000004	27
CCGATCA	50	2.1827873E-11	45.0	11
CGTTTTT	13160	0.0	42.418312	1
CGTAAGG	555	0.0	39.72973	2
GTCGATA	40	3.4607E-7	39.375004	11
ATTAGCG	140	0.0	38.571426	1
ACGCGAT	30	1.1403602E-4	37.500004	35
GCGCTAG	220	0.0	36.81818	1
CGTTCGG	640	0.0	36.21094	45
ATAGCGC	25	0.0021076484	36.0	12
TACGGGT	325	0.0	36.0	4
GTACGAG	225	0.0	36.0	1
CGCACGG	335	0.0	35.597015	2
ACCGCGT	95	0.0	35.526318	25
CTAAACG	70	2.1827873E-11	35.357143	1
CACGTGA	1905	0.0	35.314957	43
CGAAAGG	345	0.0	35.217392	2
AGGGCGA	2215	0.0	35.14673	6
GCGAGAC	2055	0.0	35.036495	21
ACGTGCG	135	0.0	35.0	1
>>END_MODULE