FastQCFastQC Report
Sat 18 Jun 2016
SRR3550407_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550407_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748589
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147630.8442807314926493No Hit
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG44440.25414777286143286No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTT41880.2395073971070389No Hit
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC38480.22006314805823438Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCT36330.2077675199832551No Hit
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT32360.1850634997703863Illumina Single End Adapter 1 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTC31100.1778576898287705No Hit
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC28810.1647614162047228Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCC22970.13136305901501152No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTC18800.10751525944633074No Hit
CGTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT18550.1060855352515657No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGA207.0342957E-445.0000048
ACGTCGT207.0342957E-445.0000049
GCGTAGT207.0342957E-445.00000414
CGCTTAA207.0342957E-445.00000424
CGTTTTT111700.042.9655341
CGCATCG800.042.18750421
CTATCGG603.6379788E-1241.2499962
GCTATCG603.6379788E-1241.2499961
CACAACG2600.040.67307712
GTATCCG451.9292202E-840.01
CGAACGC403.4596087E-739.37500418
TAGTACG750.039.01
CGACTAA700.038.5714338
AACGAGC2650.038.20754615
CGTTTCT4500.038.01
TAATCGT1250.037.80000321
TCGTTTA900.037.538
TAACGCC900.037.512
TCACGAC601.5643309E-1037.49999625
TCGTTGA1200.037.49999624