##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550407_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1748589
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17854109799387	33.0	31.0	34.0	30.0	34.0
2	32.38307286617953	34.0	31.0	34.0	30.0	34.0
3	32.325609391343534	34.0	31.0	34.0	30.0	34.0
4	35.88918207766376	37.0	35.0	37.0	35.0	37.0
5	35.93563152919297	37.0	35.0	37.0	35.0	37.0
6	35.99097043387554	37.0	35.0	37.0	35.0	37.0
7	36.120143155424174	37.0	35.0	37.0	35.0	37.0
8	36.051371134097266	37.0	35.0	37.0	35.0	37.0
9	37.609797385206015	39.0	38.0	39.0	35.0	39.0
10	37.37729220531526	39.0	37.0	39.0	34.0	39.0
11	37.373445675341664	39.0	37.0	39.0	35.0	39.0
12	37.298192428295046	39.0	37.0	39.0	34.0	39.0
13	37.312128235966256	39.0	37.0	39.0	34.0	39.0
14	38.56768686066308	40.0	38.0	41.0	34.0	41.0
15	38.62360909281712	40.0	38.0	41.0	35.0	41.0
16	38.630814902758736	40.0	38.0	41.0	35.0	41.0
17	38.63434860907852	40.0	38.0	41.0	34.0	41.0
18	38.48746446420514	40.0	38.0	41.0	34.0	41.0
19	38.318204563794005	40.0	37.0	41.0	34.0	41.0
20	38.17092524315319	40.0	36.0	41.0	34.0	41.0
21	38.10602205549732	40.0	36.0	41.0	34.0	41.0
22	38.08203071161948	40.0	36.0	41.0	34.0	41.0
23	38.00716177443642	40.0	36.0	41.0	34.0	41.0
24	37.96449594501624	40.0	36.0	41.0	34.0	41.0
25	37.898884757939115	40.0	36.0	41.0	34.0	41.0
26	37.869825899625354	40.0	36.0	41.0	34.0	41.0
27	37.8469119959007	40.0	36.0	41.0	34.0	41.0
28	37.74997269226788	40.0	36.0	41.0	33.0	41.0
29	37.7324328358465	40.0	36.0	41.0	33.0	41.0
30	37.61191795213169	40.0	35.0	41.0	33.0	41.0
31	37.45800985823427	40.0	35.0	41.0	33.0	41.0
32	37.22033651132428	40.0	35.0	41.0	32.0	41.0
33	36.98842666858822	40.0	35.0	41.0	31.0	41.0
34	36.67997168002315	40.0	35.0	41.0	31.0	41.0
35	36.52474194908009	40.0	35.0	41.0	30.0	41.0
36	36.395689896253494	39.0	35.0	41.0	30.0	41.0
37	36.31228779318639	39.0	35.0	41.0	30.0	41.0
38	36.224471845585214	39.0	35.0	41.0	30.0	41.0
39	36.109284686109774	39.0	35.0	41.0	29.0	41.0
40	36.04164786579351	39.0	35.0	41.0	29.0	41.0
41	35.950380563986165	39.0	35.0	41.0	29.0	41.0
42	35.86627446472556	39.0	35.0	41.0	28.0	41.0
43	35.77947076185427	39.0	35.0	41.0	28.0	41.0
44	35.703348242497235	39.0	35.0	41.0	27.0	41.0
45	35.58667817308699	39.0	35.0	41.0	27.0	41.0
46	35.4934681620438	38.0	35.0	41.0	26.0	41.0
47	35.42699856856014	38.0	35.0	40.0	26.0	41.0
48	35.35764093220305	38.0	35.0	40.0	26.0	41.0
49	35.278572037225445	38.0	35.0	40.0	26.0	41.0
50	35.193999275987665	38.0	35.0	40.0	26.0	41.0
51	34.14051500953054	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	11.0
10	16.0
11	18.0
12	16.0
13	18.0
14	30.0
15	46.0
16	88.0
17	165.0
18	362.0
19	790.0
20	1435.0
21	2179.0
22	3838.0
23	6225.0
24	10452.0
25	18073.0
26	25313.0
27	27115.0
28	24738.0
29	24944.0
30	27093.0
31	32108.0
32	39880.0
33	53748.0
34	94941.0
35	129365.0
36	126525.0
37	185342.0
38	334046.0
39	579259.0
40	408.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.35007025664693	20.67055208513836	24.288554943442968	15.690822714771738
2	36.42176634989697	22.558131156034953	23.794556639667753	17.22554585440032
3	29.652822933233598	22.504087581472835	31.553727033625396	16.289362451668175
4	25.69471728347828	24.414656617421247	31.253999653434857	18.63662644566562
5	22.952792222757893	29.134462129179582	29.52775066067555	18.384994987386975
6	23.695619725390017	33.344828315859246	28.82015156220244	14.139400396548302
7	77.84253475230601	5.175029695371525	12.51712094723231	4.465314605090161
8	78.87599658924997	4.1013068251029825	12.565502813983159	4.457193771663896
9	73.11495154092815	5.921517291942246	14.764704570370741	6.198826596758873
10	36.546095165873744	22.598678134198487	24.129683990920682	16.72554270900709
11	28.9694719571037	24.14987169655076	26.8872216398479	19.99343470649764
12	23.739140529878664	22.59427458367861	32.847856185758914	20.81872870068381
13	23.008437088418145	23.507239265487772	34.68047665860874	18.803846987485336
14	20.790934862337576	26.493304029706238	33.469385887707176	19.246375220249014
15	20.09334383322782	25.24601264219322	35.541914080438566	19.11872944414039
16	21.84172495652209	24.5834784503391	33.24005812686686	20.334738466271947
17	21.7034992213722	25.04453590866693	31.304325945090582	21.94763892487028
18	23.145862178018962	24.59766131435117	32.23547671865716	20.02099978897271
19	21.937802422410297	27.581095386051267	30.047312433053165	20.433789758485272
20	23.22358198524639	26.705017588467044	30.000474668432663	20.070925757853907
21	21.645738363903696	27.558848877580722	31.46228187412823	19.333130884387355
22	21.331027474152016	24.92678382398608	30.420413258919048	23.32177544294285
23	20.901080814302276	26.264433780608247	30.260055393234204	22.574430011855274
24	21.70121166266058	24.823386170220672	31.795007288733945	21.680394878384803
25	20.766972684833316	26.52155537979479	29.759194413324114	22.952277522047776
26	19.446479418548325	27.373556621939176	31.49030446834562	21.689659491166875
27	21.14853747793221	25.544710620963528	30.728032716664693	22.57871918443957
28	19.990346502236946	25.84626804812337	30.919101058053094	23.244284391586586
29	21.247016880467623	23.826067760920377	31.60456802599124	23.322347332620758
30	21.734895964689244	24.37308023783748	31.189604875702635	22.70241892177064
31	22.660041896637804	25.247556744323568	29.053025039045764	23.03937631999286
32	21.902402451347918	24.777978129794935	29.83313974867736	23.486479670179786
33	22.1635272782798	25.011881008058495	28.513447127941443	24.311144585720257
34	20.7427245624901	26.046315057454894	30.39845269528746	22.812507684767546
35	20.92321294483724	25.39453239154541	29.910973933840367	23.77128072977698
36	21.797231939581	26.682427946189758	29.56749699329002	21.952843120939225
37	22.30907320130688	26.792459520218877	28.9353873323005	21.963079946173742
38	21.824225132378164	26.651202769776088	28.55027682319859	22.974295274647158
39	21.7175105184809	25.273063023958176	30.145963402491954	22.863463055068973
40	22.163870412086546	25.05665996983854	30.278698996733937	22.50077062134098
41	20.474279547681014	25.77678345225779	29.593975485377065	24.154961514684125
42	21.63458651518453	24.576958908010972	30.091119182380766	23.69733539442373
43	21.90480438799512	24.122535369946853	30.325022060644326	23.6476381814137
44	21.786251657765206	24.25069584676559	28.733567464967468	25.229485030501735
45	21.683883405420026	24.048361278722442	28.957691029738836	25.310064286118696
46	22.390510291440698	24.794734497357585	29.070639241125274	23.744115970076443
47	20.712528787496662	24.880346382140115	31.010603406518054	23.39652142384517
48	21.0007039961935	23.764475242609898	30.788996156329475	24.445824604867123
49	22.132359290833925	22.65895530624978	31.074083160765625	24.13460224215067
50	19.90988162455557	22.907212615428783	31.185544458989504	25.99736130102614
51	20.294648999850736	22.782197531838527	29.65791275136696	27.26524071694378
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	577.0
1	1106.5
2	1636.0
3	12688.0
4	23740.0
5	16345.0
6	8950.0
7	9014.5
8	9079.0
9	9550.0
10	10021.0
11	10235.0
12	10449.0
13	10565.0
14	10681.0
15	10499.5
16	10318.0
17	10195.5
18	10073.0
19	9719.5
20	9366.0
21	9078.5
22	8791.0
23	9077.5
24	9364.0
25	10205.5
26	11815.0
27	12583.0
28	14451.0
29	16319.0
30	19117.0
31	21915.0
32	25464.5
33	29014.0
34	35171.0
35	41328.0
36	46012.0
37	50696.0
38	54680.5
39	58665.0
40	66288.0
41	73911.0
42	81750.5
43	89590.0
44	100762.5
45	111935.0
46	134716.5
47	157498.0
48	170256.5
49	183015.0
50	181159.5
51	179304.0
52	157071.5
53	134839.0
54	117600.5
55	100362.0
56	90429.5
57	80497.0
58	71010.5
59	61524.0
60	59419.0
61	57314.0
62	52556.5
63	47799.0
64	41217.5
65	34636.0
66	29524.5
67	24413.0
68	20816.0
69	17219.0
70	14104.0
71	10989.0
72	9287.5
73	7586.0
74	6263.0
75	3798.0
76	2656.0
77	2362.0
78	2068.0
79	1429.0
80	790.0
81	707.0
82	624.0
83	455.0
84	286.0
85	204.5
86	123.0
87	69.5
88	16.0
89	21.0
90	26.0
91	15.0
92	4.0
93	6.5
94	9.0
95	4.5
96	0.0
97	2.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1748589.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.94578035620626
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.93353049758	22.569850153807806
2	11.434044615621989	7.076708665162017
3	4.382187010667129	4.068305901357752
4	2.1930775153206477	2.714659803729893
5	1.3598455208900124	2.104074040391661
6	0.9078201792372431	1.6855922321764514
7	0.6531234121488506	1.4147989560498013
8	0.5360698925645857	1.3271280920703004
9	0.429120716933848	1.1951527907279358
>10	4.184419048106274	28.90792960804275
>50	0.8707412263917221	18.52861492151009
>100	0.11175082856276335	5.224084942677317
>500	0.0016706879902497302	0.3203722744461561
>1k	0.002413215985916277	2.0146630871617606
>5k	0.0	0.0
>10k+	1.8563199891663668E-4	0.8480645306882915
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14763	0.8442807314926493	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG	4444	0.25414777286143286	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTT	4188	0.2395073971070389	No Hit
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	3848	0.22006314805823438	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCT	3633	0.2077675199832551	No Hit
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT	3236	0.1850634997703863	Illumina Single End Adapter 1 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTC	3110	0.1778576898287705	No Hit
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	2881	0.1647614162047228	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCC	2297	0.13136305901501152	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTC	1880	0.10751525944633074	No Hit
CGTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT	1855	0.1060855352515657	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.287558711624058E-4	0.0	0.0	0.19369903390676713	0.0
2	2.287558711624058E-4	0.0	0.0	0.718179057514373	0.0
3	2.287558711624058E-4	0.0	0.0	1.2122917392251695	0.0
4	2.287558711624058E-4	0.0	0.0	1.6979976426707477	0.0
5	2.287558711624058E-4	0.0	0.0	3.1000423770251326	0.0
6	2.287558711624058E-4	0.0	0.0	4.358142479450574	0.0
7	2.287558711624058E-4	0.0	0.0	5.44553351302107	0.0
8	2.287558711624058E-4	0.0	0.0	6.986719006009989	0.0
9	2.287558711624058E-4	0.0	0.0	7.675388556144411	0.0
10	2.287558711624058E-4	0.0	0.0	9.009149662956819	0.0
11	2.8594483895300727E-4	0.0	0.0	10.809629935908324	0.0
12	2.8594483895300727E-4	0.0	0.0	12.093465073839536	0.0
13	2.8594483895300727E-4	0.0	0.0	12.677021301174833	0.0
14	2.8594483895300727E-4	0.0	0.0	12.943350324175665	0.0
15	2.8594483895300727E-4	0.0	0.0	13.309302529067722	0.0
16	2.8594483895300727E-4	0.0	0.0	14.092047931217685	0.0
17	2.8594483895300727E-4	0.0	0.0	15.03475087627796	0.0
18	3.431338067436087E-4	0.0	0.0	16.023262184538506	0.0
19	3.431338067436087E-4	0.0	0.0	16.63524132886573	0.0
20	4.0032277453421015E-4	0.0	0.0	17.209876077225694	0.0
21	4.0032277453421015E-4	0.0	0.0	17.95676399657095	0.0
22	4.0032277453421015E-4	0.0	0.0	18.781486101079214	0.0
23	4.0032277453421015E-4	0.0	0.0	19.620448258567336	0.0
24	4.0032277453421015E-4	0.0	0.0	20.29207549630016	0.0
25	4.0032277453421015E-4	0.0	0.0	20.88718389512916	0.0
26	4.0032277453421015E-4	0.0	0.0	21.4522108969003	0.0
27	4.575117423248116E-4	0.0	0.0	21.995334524007642	0.0
28	4.575117423248116E-4	0.0	0.0	22.5560151642267	0.0
29	4.575117423248116E-4	0.0	0.0	23.160216608934405	0.0
30	4.575117423248116E-4	0.0	0.0	23.838534955898727	0.0
31	4.575117423248116E-4	0.0	0.0	24.455489540423734	0.0
32	4.575117423248116E-4	0.0	0.0	25.05208485241529	0.0
33	4.575117423248116E-4	0.0	0.0	25.630722828520597	0.0
34	4.575117423248116E-4	0.0	0.0	26.203413151975678	0.0
35	4.575117423248116E-4	0.0	0.0	26.780335459047265	0.0
36	4.575117423248116E-4	0.0	0.0	27.38333593543137	0.0
37	4.575117423248116E-4	0.0	0.0	27.956312203725403	0.0
38	4.575117423248116E-4	0.0	0.0	28.552335626039053	0.0
39	4.575117423248116E-4	0.0	0.0	29.152705409904787	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGA	20	7.0342957E-4	45.000004	8
ACGTCGT	20	7.0342957E-4	45.000004	9
GCGTAGT	20	7.0342957E-4	45.000004	14
CGCTTAA	20	7.0342957E-4	45.000004	24
CGTTTTT	11170	0.0	42.965534	1
CGCATCG	80	0.0	42.187504	21
CTATCGG	60	3.6379788E-12	41.249996	2
GCTATCG	60	3.6379788E-12	41.249996	1
CACAACG	260	0.0	40.673077	12
GTATCCG	45	1.9292202E-8	40.0	1
CGAACGC	40	3.4596087E-7	39.375004	18
TAGTACG	75	0.0	39.0	1
CGACTAA	70	0.0	38.57143	38
AACGAGC	265	0.0	38.207546	15
CGTTTCT	450	0.0	38.0	1
TAATCGT	125	0.0	37.800003	21
TCGTTTA	90	0.0	37.5	38
TAACGCC	90	0.0	37.5	12
TCACGAC	60	1.5643309E-10	37.499996	25
TCGTTGA	120	0.0	37.499996	24
>>END_MODULE