FastQCFastQC Report
Sat 18 Jun 2016
SRR3550401_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550401_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences965705
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT361003.738201624719764No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT32900.3406837491780616No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT24920.25804981852636155No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC22870.23682180375994738No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21230.21983939194681607No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCC15380.15926188639387803No Hit
CCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC14620.1513919882365733TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTC14050.14548956461859472No Hit
CGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG13690.1417617181230293TruSeq Adapter, Index 13 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT12650.130992383802507No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC12190.12622902439150674No Hit
CTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGCT12090.12519351147607188TruSeq Adapter, Index 13 (95% over 23bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGT10640.11017857420226673No Hit
GCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC10530.10903950999528841TruSeq Adapter, Index 19 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGGA253.8903403E-545.0000043
CGTTCAT253.8903403E-545.00000417
TCTACGG406.8139343E-945.0000042
CGTAGCG253.8903403E-545.0000042
AACGCGA207.0327247E-445.00000421
AATTCGG207.0327247E-445.0000042
TCGTAAG302.1651394E-645.0000041
ACGTATC207.0327247E-445.00000420
CGGTCTA207.0327247E-445.00000431
TACGTAG302.1651394E-645.0000041
CGTTATT13650.044.5054931
CGTTTTT202200.044.2544561
GCGAGAC650.041.5384621
AGACACG650.041.5384624
AGTTGCG603.6379788E-1241.2500041
GCGATAC501.0804797E-940.5000049
CGTTTCT4400.040.3977281
GCGTTAG451.927765E-840.01
CGCACGG451.927765E-840.02
GTTAGCG403.4574623E-739.3750041