Basic Statistics
Measure | Value |
---|---|
Filename | SRR3550401_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 965705 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 37 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 36100 | 3.738201624719764 | No Hit |
CGTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT | 3290 | 0.3406837491780616 | No Hit |
CGTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT | 2492 | 0.25804981852636155 | No Hit |
CGTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC | 2287 | 0.23682180375994738 | No Hit |
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 2123 | 0.21983939194681607 | No Hit |
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCC | 1538 | 0.15926188639387803 | No Hit |
CCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC | 1462 | 0.1513919882365733 | TruSeq Adapter, Index 19 (95% over 22bp) |
CGTTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTC | 1405 | 0.14548956461859472 | No Hit |
CGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG | 1369 | 0.1417617181230293 | TruSeq Adapter, Index 13 (95% over 21bp) |
CGTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT | 1265 | 0.130992383802507 | No Hit |
GAATCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC | 1219 | 0.12622902439150674 | No Hit |
CTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGCT | 1209 | 0.12519351147607188 | TruSeq Adapter, Index 13 (95% over 23bp) |
CGTTTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGT | 1064 | 0.11017857420226673 | No Hit |
GCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC | 1053 | 0.10903950999528841 | TruSeq Adapter, Index 19 (95% over 22bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCACGGA | 25 | 3.8903403E-5 | 45.000004 | 3 |
CGTTCAT | 25 | 3.8903403E-5 | 45.000004 | 17 |
TCTACGG | 40 | 6.8139343E-9 | 45.000004 | 2 |
CGTAGCG | 25 | 3.8903403E-5 | 45.000004 | 2 |
AACGCGA | 20 | 7.0327247E-4 | 45.000004 | 21 |
AATTCGG | 20 | 7.0327247E-4 | 45.000004 | 2 |
TCGTAAG | 30 | 2.1651394E-6 | 45.000004 | 1 |
ACGTATC | 20 | 7.0327247E-4 | 45.000004 | 20 |
CGGTCTA | 20 | 7.0327247E-4 | 45.000004 | 31 |
TACGTAG | 30 | 2.1651394E-6 | 45.000004 | 1 |
CGTTATT | 1365 | 0.0 | 44.505493 | 1 |
CGTTTTT | 20220 | 0.0 | 44.254456 | 1 |
GCGAGAC | 65 | 0.0 | 41.53846 | 21 |
AGACACG | 65 | 0.0 | 41.53846 | 24 |
AGTTGCG | 60 | 3.6379788E-12 | 41.250004 | 1 |
GCGATAC | 50 | 1.0804797E-9 | 40.500004 | 9 |
CGTTTCT | 440 | 0.0 | 40.397728 | 1 |
GCGTTAG | 45 | 1.927765E-8 | 40.0 | 1 |
CGCACGG | 45 | 1.927765E-8 | 40.0 | 2 |
GTTAGCG | 40 | 3.4574623E-7 | 39.375004 | 1 |