##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550401_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	965705
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0448159634671	33.0	31.0	34.0	30.0	34.0
2	32.16191487048322	33.0	31.0	34.0	30.0	34.0
3	31.91397994211483	33.0	31.0	34.0	30.0	34.0
4	35.77641929988972	37.0	35.0	37.0	35.0	37.0
5	33.7308039204519	37.0	35.0	37.0	32.0	37.0
6	34.73972486421837	37.0	35.0	37.0	32.0	37.0
7	35.8281845905323	37.0	35.0	37.0	33.0	37.0
8	35.86898276388752	37.0	35.0	37.0	35.0	37.0
9	37.85292713613371	39.0	38.0	39.0	35.0	39.0
10	37.42990043543318	39.0	37.0	39.0	34.0	39.0
11	37.41302468145034	39.0	37.0	39.0	34.0	39.0
12	37.51652833939971	39.0	37.0	39.0	35.0	39.0
13	37.57734194189737	39.0	37.0	39.0	35.0	39.0
14	38.8422727437468	40.0	38.0	41.0	35.0	41.0
15	38.941760682610116	40.0	38.0	41.0	35.0	41.0
16	38.886336924837295	40.0	38.0	41.0	35.0	41.0
17	38.850072227025855	40.0	38.0	41.0	35.0	41.0
18	38.48173406992819	39.0	38.0	41.0	35.0	41.0
19	38.03673067862339	39.0	37.0	41.0	34.0	41.0
20	37.712477412874534	39.0	35.0	41.0	34.0	41.0
21	37.56879067624171	39.0	35.0	41.0	34.0	41.0
22	37.58955685224784	39.0	35.0	41.0	34.0	41.0
23	37.581817428717876	39.0	35.0	41.0	34.0	41.0
24	37.487450101221384	39.0	35.0	41.0	34.0	41.0
25	37.283436453161166	39.0	35.0	41.0	33.0	41.0
26	37.33082877276187	39.0	35.0	41.0	33.0	41.0
27	37.30957487017257	39.0	35.0	41.0	33.0	41.0
28	37.17554325596326	39.0	35.0	41.0	33.0	41.0
29	37.063804163797435	39.0	35.0	41.0	33.0	41.0
30	36.7189897535997	39.0	35.0	41.0	33.0	41.0
31	36.340488037237044	39.0	35.0	41.0	31.0	41.0
32	35.76715767237407	39.0	35.0	41.0	27.0	41.0
33	34.995147586478275	39.0	35.0	41.0	21.0	41.0
34	34.331907777219755	39.0	35.0	41.0	15.0	41.0
35	33.896694125017476	39.0	34.0	41.0	12.0	41.0
36	33.58781304849825	39.0	34.0	41.0	10.0	41.0
37	33.46286184704439	39.0	33.0	41.0	10.0	41.0
38	33.31807125364371	38.0	33.0	41.0	10.0	41.0
39	33.22043688289902	38.0	33.0	41.0	10.0	41.0
40	33.11218436271946	38.0	33.0	41.0	10.0	41.0
41	32.964768744078164	38.0	33.0	41.0	10.0	41.0
42	32.89150102774657	38.0	33.0	41.0	9.0	41.0
43	32.78778198311078	38.0	32.0	41.0	8.0	41.0
44	32.614624548904686	38.0	31.0	41.0	8.0	41.0
45	32.5742913208485	38.0	31.0	40.0	8.0	41.0
46	32.46263092766424	38.0	31.0	40.0	8.0	41.0
47	32.42092460948219	38.0	31.0	40.0	8.0	41.0
48	32.32262440393288	38.0	31.0	40.0	8.0	41.0
49	32.21917769919385	38.0	31.0	40.0	8.0	41.0
50	32.12921337261379	37.0	30.0	40.0	8.0	41.0
51	31.22764405279045	36.0	27.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	10.0
9	7.0
10	10.0
11	21.0
12	20.0
13	15.0
14	25.0
15	50.0
16	114.0
17	222.0
18	466.0
19	900.0
20	1620.0
21	2612.0
22	4272.0
23	7181.0
24	12768.0
25	23697.0
26	35207.0
27	37825.0
28	32194.0
29	25816.0
30	22501.0
31	21943.0
32	23486.0
33	28286.0
34	42630.0
35	58643.0
36	62632.0
37	87932.0
38	158249.0
39	274178.0
40	171.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.663510078129452	17.69608731444903	20.643157071776578	31.997245535644943
2	46.198373209209855	19.687275099538677	20.538156062151486	13.576195629099985
3	23.98682827571567	18.9281405812334	44.04492055027156	13.040110592779369
4	21.68622923149409	19.928446057543454	44.151371277978264	14.233953432984192
5	18.15947934410612	28.177652595772003	39.8554423970053	13.807425663116582
6	20.231126482725056	26.90096872233239	41.2792726557282	11.588632139214356
7	63.086656898328165	4.523120414619371	28.698722694818812	3.691499992233653
8	64.67264847960817	4.147436328899612	27.82537110194107	3.354544089551157
9	60.053121812561805	6.145769153105762	29.271671990928905	4.529437043403524
10	27.995195220072382	25.090581492277664	36.09570210364449	10.818521184005467
11	19.465675335635623	20.80231540687891	44.56650840577609	15.165500851709373
12	18.491878989960703	18.369481363356304	47.081458623492686	16.05718102319031
13	17.024660740081078	19.661801481818983	47.54712878156373	15.76640899653621
14	14.580953810946406	22.44422468559239	46.175591925070286	16.799229578390918
15	14.20060991710719	23.557815274851016	47.05857378806157	15.183001019980221
16	16.26915051697982	22.3868572700773	45.62666652859828	15.7173256843446
17	15.74766621276684	23.369662578116507	44.09410741375472	16.788563795361938
18	15.834131541205648	23.46337649696336	44.64707131059692	16.055420651234073
19	16.196871715482473	24.61041415339053	43.313951983266115	15.87876214786089
20	17.124898390295172	23.55170574864995	44.56733681610844	14.756059044946438
21	16.612319497154928	23.52260783572623	44.82507598076017	15.03999668635867
22	16.242330732470062	21.65298926690863	44.15696304772162	17.947716952899697
23	15.205989406702875	22.777763395653952	45.21059743917656	16.80564975846661
24	15.483299765456323	22.075685638989132	45.95016076338012	16.49085383217442
25	15.564691080609503	24.34563350091384	43.669857772301064	16.41981764617559
26	15.112896795605282	24.850860252354497	43.70993212212839	16.326310829911826
27	15.782562998016994	23.66550861805624	43.906265370894836	16.645663013031932
28	14.709357412460328	23.300593866657003	44.930905400717606	17.05914332016506
29	15.319170968359902	21.87562454372712	44.97781413578681	17.827390352126166
30	15.57142191455983	22.662925013332227	44.14495109790257	17.620701974205375
31	15.435251966180147	23.149719634878146	43.19403958765876	18.220988811282947
32	15.19542717496544	23.514427283694296	43.08955633449138	18.200589206848882
33	15.203297073122746	25.10487157051066	40.602668516783076	19.08916283958352
34	14.868515747562661	25.293334921119804	40.41845076912722	19.419698562190316
35	15.829575284377734	24.495989976234977	38.5149709279749	21.159463811412387
36	16.0835866025339	26.175074168612568	36.491371588632134	21.249967640221392
37	15.687088707213901	26.460047322940234	37.60340890851761	20.249455061328252
38	15.85919095375917	26.69220931858073	37.265831698085854	20.182768029574248
39	17.15368564934426	25.58524601198088	36.70603341600178	20.555034922673073
40	17.572964828803826	25.72845744818552	36.69195044035187	20.006627282658783
41	15.82325865559358	26.256258381182658	36.069503626883986	21.850979336339773
42	16.685633811567715	26.397295240264885	36.00074556929911	20.91632537886829
43	17.44290440662521	25.673368161084387	36.351266691173805	20.532460741116594
44	17.266142351960482	26.110561713980978	35.84614349102469	20.777152443033845
45	17.19676298662635	25.431679446621896	35.70396756773549	21.66758999901626
46	17.20597905157372	26.13997028077933	35.267395322588165	21.38665534505879
47	16.5205730528474	25.001423830258723	37.432963482637035	21.045039634256838
48	16.543664990861597	24.477454295048695	37.116717838263234	21.862162875826467
49	16.405320465359505	24.566301303193004	37.22078688626444	21.807591345183052
50	15.679011706473508	24.37856281162467	37.649282130671374	22.293143351230448
51	15.6918520666249	23.660848809936784	36.40273168307092	24.2445674403674
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1187.0
1	1832.0
2	2477.0
3	32340.5
4	62204.0
5	42167.5
6	22131.0
7	21284.5
8	20438.0
9	20032.5
10	19627.0
11	19239.0
12	18851.0
13	18011.0
14	17171.0
15	16553.5
16	15936.0
17	14731.0
18	13526.0
19	12714.0
20	11902.0
21	11496.5
22	11091.0
23	10718.0
24	10345.0
25	10931.0
26	12655.5
27	13794.0
28	15269.5
29	16745.0
30	18658.0
31	20571.0
32	23152.0
33	25733.0
34	27742.5
35	29752.0
36	32408.0
37	35064.0
38	36782.0
39	38500.0
40	41660.5
41	44821.0
42	47807.5
43	50794.0
44	54861.5
45	58929.0
46	68590.0
47	78251.0
48	77851.5
49	77452.0
50	71838.5
51	66225.0
52	55268.0
53	44311.0
54	37895.5
55	31480.0
56	27410.0
57	23340.0
58	21218.5
59	19097.0
60	17818.0
61	16539.0
62	14838.5
63	13138.0
64	10758.5
65	8379.0
66	6594.5
67	4810.0
68	4144.0
69	3478.0
70	2859.0
71	2240.0
72	1871.5
73	1503.0
74	1221.5
75	841.5
76	743.0
77	540.0
78	337.0
79	287.0
80	237.0
81	146.0
82	55.0
83	37.5
84	20.0
85	16.0
86	12.0
87	9.0
88	6.0
89	4.0
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	965705.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.01870607272529
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.34295194852365	31.351835668976086
2	9.07383364788398	7.2624616341494725
3	3.2980360393096686	3.959494046231661
4	1.782569143624371	2.8534444245205326
5	1.0994048133987047	2.199837904117107
6	0.7843599902721071	1.8833443143543072
7	0.5917166156938237	1.6575813325259214
8	0.5001760829576116	1.6013119718790176
9	0.4096347321625897	1.4753746749234802
>10	3.8996047776004983	31.888898377733142
>50	0.1779157363591825	4.52626556773245
>100	0.032773565353646186	2.3337363743973296
>500	0.0033813995993542396	0.9820012914218801
>1k	0.0033813995993542396	2.2667013061270787
>5k	0.0	0.0
>10k+	2.601076614887877E-4	3.757711110910523
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36100	3.738201624719764	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT	3290	0.3406837491780616	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT	2492	0.25804981852636155	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC	2287	0.23682180375994738	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2123	0.21983939194681607	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCC	1538	0.15926188639387803	No Hit
CCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	1462	0.1513919882365733	TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTC	1405	0.14548956461859472	No Hit
CGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG	1369	0.1417617181230293	TruSeq Adapter, Index 13 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	1265	0.130992383802507	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC	1219	0.12622902439150674	No Hit
CTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGCT	1209	0.12519351147607188	TruSeq Adapter, Index 13 (95% over 23bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGT	1064	0.11017857420226673	No Hit
GCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	1053	0.10903950999528841	TruSeq Adapter, Index 19 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0710258308696754E-4	0.0	0.0	0.13440957642344195	0.0
2	2.0710258308696754E-4	0.0	0.0	0.5294577536618326	0.0
3	2.0710258308696754E-4	0.0	0.0	0.8444607825371102	0.0
4	2.0710258308696754E-4	0.0	0.0	1.260115666792654	0.0
5	2.0710258308696754E-4	0.0	0.0	2.3765021409229528	0.0
6	2.0710258308696754E-4	0.0	0.0	3.456231457846858	0.0
7	2.0710258308696754E-4	0.0	0.0	4.336003230800296	0.0
8	2.0710258308696754E-4	0.0	0.0	5.311145743265283	0.0
9	2.0710258308696754E-4	0.0	0.0	5.764079092476481	0.0
10	2.0710258308696754E-4	0.0	0.0	6.65337758425192	0.0
11	2.0710258308696754E-4	0.0	0.0	7.743254927747087	0.0
12	2.0710258308696754E-4	0.0	0.0	8.597553082980827	0.0
13	2.0710258308696754E-4	0.0	0.0	8.95459793622276	0.0
14	2.0710258308696754E-4	0.0	0.0	9.124111400479443	0.0
15	2.0710258308696754E-4	0.0	0.0	9.348403497962629	0.0
16	2.0710258308696754E-4	0.0	0.0	9.776277434620303	0.0
17	2.0710258308696754E-4	0.0	0.0	10.37511455361627	0.0
18	2.0710258308696754E-4	0.0	0.0	11.00139276487126	0.0
19	2.0710258308696754E-4	0.0	0.0	11.424296239534847	0.0
20	2.0710258308696754E-4	0.0	0.0	11.805986300164129	0.0
21	2.0710258308696754E-4	0.0	0.0	12.307278102526134	0.0
22	2.0710258308696754E-4	0.0	0.0	12.857549665788207	0.0
23	2.0710258308696754E-4	0.0	0.0	13.405128895470149	0.0
24	2.0710258308696754E-4	0.0	0.0	13.846671602611563	0.0
25	2.0710258308696754E-4	0.0	0.0	14.232400163611041	0.0
26	2.0710258308696754E-4	0.0	0.0	14.591516042683843	0.0
27	3.106538746304513E-4	0.0	0.0	14.971238628773797	0.0
28	3.106538746304513E-4	0.0	0.0	15.355103266525491	0.0
29	3.106538746304513E-4	0.0	0.0	15.782252344142362	0.0
30	3.106538746304513E-4	0.0	0.0	16.230215231359473	0.0
31	3.106538746304513E-4	0.0	0.0	16.671343733334712	0.0
32	3.106538746304513E-4	0.0	0.0	17.084616937884757	0.0
33	3.106538746304513E-4	0.0	0.0	17.482150346120193	0.0
34	3.106538746304513E-4	0.0	0.0	17.848307713017952	0.0
35	3.106538746304513E-4	0.0	0.0	18.24221682604936	0.0
36	3.106538746304513E-4	0.0	0.0	18.627531181882667	0.0
37	3.106538746304513E-4	0.0	0.0	19.020404781998643	0.0
38	4.142051661739351E-4	0.0	0.0	19.408307920120535	0.0
39	4.142051661739351E-4	0.0	0.0	19.792690314329946	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCACGGA	25	3.8903403E-5	45.000004	3
CGTTCAT	25	3.8903403E-5	45.000004	17
TCTACGG	40	6.8139343E-9	45.000004	2
CGTAGCG	25	3.8903403E-5	45.000004	2
AACGCGA	20	7.0327247E-4	45.000004	21
AATTCGG	20	7.0327247E-4	45.000004	2
TCGTAAG	30	2.1651394E-6	45.000004	1
ACGTATC	20	7.0327247E-4	45.000004	20
CGGTCTA	20	7.0327247E-4	45.000004	31
TACGTAG	30	2.1651394E-6	45.000004	1
CGTTATT	1365	0.0	44.505493	1
CGTTTTT	20220	0.0	44.254456	1
GCGAGAC	65	0.0	41.53846	21
AGACACG	65	0.0	41.53846	24
AGTTGCG	60	3.6379788E-12	41.250004	1
GCGATAC	50	1.0804797E-9	40.500004	9
CGTTTCT	440	0.0	40.397728	1
GCGTTAG	45	1.927765E-8	40.0	1
CGCACGG	45	1.927765E-8	40.0	2
GTTAGCG	40	3.4574623E-7	39.375004	1
>>END_MODULE