##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550400_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	378485
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20646260750096	33.0	31.0	34.0	30.0	34.0
2	32.320091945519636	34.0	31.0	34.0	30.0	34.0
3	32.081448406145554	33.0	31.0	34.0	30.0	34.0
4	35.90074111259363	37.0	35.0	37.0	35.0	37.0
5	33.836976366302494	37.0	35.0	37.0	32.0	37.0
6	34.82144602824418	37.0	35.0	37.0	32.0	37.0
7	35.8748669035761	37.0	35.0	37.0	33.0	37.0
8	35.91443254025919	37.0	35.0	37.0	35.0	37.0
9	37.906788908411166	39.0	38.0	39.0	35.0	39.0
10	37.53937408351718	39.0	37.0	39.0	35.0	39.0
11	37.49424679974107	39.0	37.0	39.0	35.0	39.0
12	37.450749699459685	39.0	37.0	39.0	35.0	39.0
13	37.4172054374678	39.0	37.0	39.0	35.0	39.0
14	38.67071086040398	40.0	38.0	41.0	35.0	41.0
15	38.80033026407916	40.0	38.0	41.0	35.0	41.0
16	38.75835502067453	40.0	38.0	41.0	35.0	41.0
17	38.69788763094971	40.0	38.0	41.0	35.0	41.0
18	38.31840627765962	39.0	38.0	41.0	35.0	41.0
19	37.87806650197498	39.0	37.0	41.0	34.0	41.0
20	37.50347041494379	39.0	35.0	41.0	34.0	41.0
21	37.361538766397615	39.0	35.0	41.0	34.0	41.0
22	37.37261978678151	39.0	35.0	41.0	34.0	41.0
23	37.36915069289404	39.0	35.0	41.0	34.0	41.0
24	37.25957171354215	39.0	35.0	41.0	34.0	41.0
25	37.053706223496306	38.0	35.0	41.0	33.0	41.0
26	37.06535794020899	38.0	35.0	41.0	33.0	41.0
27	37.03979814259482	38.0	35.0	41.0	33.0	41.0
28	36.899959047254185	38.0	35.0	41.0	33.0	41.0
29	36.792081588438116	38.0	35.0	41.0	33.0	41.0
30	36.42457957382723	38.0	35.0	41.0	32.0	41.0
31	36.065217908239426	38.0	35.0	41.0	31.0	41.0
32	35.49583206732103	38.0	35.0	41.0	27.0	41.0
33	34.798789912413966	38.0	35.0	41.0	21.0	41.0
34	34.195468776833955	38.0	34.0	41.0	16.0	41.0
35	33.78390424983817	38.0	34.0	41.0	15.0	41.0
36	33.50567657899256	38.0	34.0	41.0	10.0	41.0
37	33.346782567340846	38.0	33.0	41.0	10.0	41.0
38	33.21312865767467	38.0	33.0	41.0	10.0	41.0
39	33.07635441298863	38.0	33.0	41.0	10.0	41.0
40	32.95005614489346	37.0	33.0	41.0	10.0	41.0
41	32.77859096133268	37.0	33.0	40.0	10.0	41.0
42	32.68592678705893	37.0	32.0	40.0	10.0	41.0
43	32.575401931384334	37.0	32.0	40.0	10.0	41.0
44	32.40254435446583	37.0	31.0	40.0	10.0	41.0
45	32.35375246046739	37.0	31.0	40.0	10.0	41.0
46	32.24672576191923	36.0	31.0	40.0	10.0	41.0
47	32.22561263986684	36.0	31.0	40.0	10.0	41.0
48	32.16762355179201	36.0	31.0	40.0	10.0	41.0
49	32.07634648665073	36.0	31.0	40.0	9.0	41.0
50	31.9974979193363	36.0	30.0	40.0	8.0	41.0
51	31.14429105512768	35.0	28.0	39.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	9.0
10	5.0
11	8.0
12	7.0
13	13.0
14	13.0
15	21.0
16	43.0
17	72.0
18	224.0
19	371.0
20	642.0
21	1100.0
22	1616.0
23	2577.0
24	4438.0
25	7661.0
26	12379.0
27	14326.0
28	13617.0
29	11397.0
30	10028.0
31	10022.0
32	10279.0
33	12760.0
34	21419.0
35	29952.0
36	24899.0
37	31957.0
38	54815.0
39	101739.0
40	73.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.3006195754125	16.712419250432646	19.414507840469238	31.572453333685612
2	47.282455051058825	19.34713396832107	20.319695628624647	13.050715351995457
3	25.354505462567868	18.37113756159425	42.91821340343739	13.35614357240049
4	23.109502358085525	19.909639747942453	43.05507483784034	13.925783056131683
5	19.21661360423795	28.377346526282416	38.79361137165277	13.612428497826862
6	21.500191553165912	27.129212518329656	40.735564157100015	10.635031771404414
7	62.50208066369869	5.446186770942045	28.826505673936882	3.2252268914223814
8	64.12618729936457	4.89741997701362	28.098339432209997	2.878053291411813
9	59.26100109647674	6.726818764283922	29.671717505317254	4.340462633922084
10	33.26842543297621	20.334491459370913	36.08016169729316	10.316921410359724
11	25.648308387386553	19.966445169557577	40.83305811326737	13.552188329788498
12	23.252440651544976	18.117494748801143	43.89183190879427	14.738232690859613
13	20.127349828923208	19.35611715127416	44.86967779436437	15.64685522543826
14	16.14568609059804	20.890656168672468	46.368548291213656	16.59510944951583
15	16.65587804008085	21.107045193336592	47.24255915029658	14.994517616285982
16	19.212650435288054	20.095116054797415	45.53099858646974	15.161234923444786
17	18.201778141802187	21.07745353184406	44.343368957818676	16.37739936853508
18	18.390689195080387	21.210616008560446	44.661215107600036	15.737479688759132
19	19.225332575927712	21.424891343117956	43.55654781563338	15.79322826532095
20	19.621121048390293	21.104138869440003	45.102447917354716	14.172292164814987
21	19.621649470916946	21.853442012232982	44.297660409263244	14.227248107586826
22	19.331809715048152	19.625348428603512	44.42263233681652	16.620209519531816
23	18.57458023435539	20.806108564408103	45.00231184855411	15.616999352682404
24	18.278135196903445	20.55748576561819	45.8578279192042	15.306551118274173
25	17.61047333447825	20.96278584356051	44.360806901198195	17.065933920763044
26	16.74703092592837	22.06084785394401	44.2759950856705	16.916126134457112
27	16.676222307356962	22.143281768101776	44.893456808063725	16.287039116477537
28	15.678560577037398	22.800375179993924	45.45543416515846	16.065630077810216
29	17.449304463849295	21.18208119212122	45.10350476240802	16.265109581621466
30	17.86887195001123	22.06454681163058	44.10769251093174	15.958888727426451
31	18.999696157047175	22.08726898027663	42.28489900524459	16.6281358574316
32	19.06522055035206	23.018613683501325	41.59134443901343	16.324821327133176
33	18.811841948822277	24.120638862834724	40.174907856321916	16.892611332021083
34	18.03664610222334	24.610486545041415	40.16090465936563	17.191962693369618
35	18.69479635916879	25.09478579071826	38.20098550801221	18.00943234210074
36	19.56167351414191	26.81691480507814	36.6220589983751	16.99935268240485
37	19.049896297079144	27.526057835845542	36.62126636458512	16.802779502490193
38	18.880801088550406	27.495409329299708	36.028640500944555	17.59514908120533
39	19.63010423134338	26.18333619562202	36.22627052591252	17.96028904712208
40	19.41397941794259	26.978612098233747	35.87222743305547	17.735181050768194
41	18.177206494312852	26.345826122567605	36.01067413503838	19.466293248081165
42	18.265188845000463	26.72074190522742	35.145646458908544	19.868422790863573
43	19.173018745789133	26.3043449542254	34.458697174260536	20.063939125724932
44	18.228463479398126	26.318348151181688	35.578688719500114	19.87449964992008
45	18.874460018230575	26.31676288360173	35.65848052102461	19.150296577143084
46	18.537590657489726	26.963552056224156	35.958888727426455	18.539968558859666
47	18.317238463875714	25.361903377941005	37.87442038654108	18.446437771642206
48	18.472594686711492	24.795434429369724	38.42530087057611	18.306670013342668
49	18.330977449568675	25.593880867141365	37.3938200985508	18.68132158473916
50	17.606245954265027	25.378020265003897	37.83161816188224	19.184115618848832
51	17.288399804483664	24.65328876970025	37.35603788789516	20.70227353792092
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	432.0
1	1112.5
2	1793.0
3	17170.0
4	32547.0
5	21079.0
6	9611.0
7	9011.5
8	8412.0
9	8074.0
10	7736.0
11	7295.0
12	6854.0
13	6616.0
14	6378.0
15	5833.5
16	5289.0
17	4820.5
18	4352.0
19	4087.5
20	3823.0
21	3710.0
22	3597.0
23	3480.5
24	3364.0
25	3302.0
26	3955.0
27	4670.0
28	5419.0
29	6168.0
30	7197.5
31	8227.0
32	8808.5
33	9390.0
34	10421.5
35	11453.0
36	11918.0
37	12383.0
38	13297.5
39	14212.0
40	14770.0
41	15328.0
42	16144.5
43	16961.0
44	17981.0
45	19001.0
46	20273.0
47	21545.0
48	21079.5
49	20614.0
50	20068.0
51	19522.0
52	18031.0
53	16540.0
54	15169.5
55	13799.0
56	13289.5
57	12780.0
58	12498.5
59	12217.0
60	12369.0
61	12521.0
62	11595.5
63	10670.0
64	8793.5
65	6917.0
66	5954.0
67	4991.0
68	4324.0
69	3657.0
70	3181.0
71	2705.0
72	2332.5
73	1960.0
74	1549.5
75	932.5
76	726.0
77	617.0
78	508.0
79	354.5
80	201.0
81	176.0
82	151.0
83	98.5
84	46.0
85	40.0
86	34.0
87	23.0
88	12.0
89	7.5
90	3.0
91	3.5
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	378485.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.179713606006786
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.31698433476136	25.985351107135024
2	6.8281108058409785	4.5310952201576775
3	2.739497566259047	2.7268723401848334
4	1.614908310080148	2.1432878091367886
5	1.1140004361511369	1.848110771423069
6	0.9012645801829422	1.794222039222478
7	0.7739704459299426	1.79760824148282
8	0.6731956173115147	1.7869150226572004
9	0.5588579179048556	1.6688471096279123
>10	6.122629608119888	41.75311943258295
>50	0.31100844580991927	6.396029199573553
>100	0.043173408929911665	2.570773463276815
>500	0.001599015145552284	0.44221229628340225
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	7.99507572776142E-4	4.555555947255469
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17173	4.537300025100071	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	963	0.2544354465830878	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	704	0.18600472938161353	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	383	0.1011929138539176	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.284225266523111E-4	0.0	0.0	0.034611675495726385	0.0
2	5.284225266523111E-4	0.0	0.0	0.20687741918437982	0.0
3	5.284225266523111E-4	0.0	0.0	0.2681744322760479	0.0
4	5.284225266523111E-4	0.0	0.0	0.3654041771800732	0.0
5	5.284225266523111E-4	0.0	0.0	0.6438828487258412	0.0
6	5.284225266523111E-4	0.0	0.0	0.7733463677556575	0.0
7	5.284225266523111E-4	0.0	0.0	0.9273815342748062	0.0
8	5.284225266523111E-4	0.0	0.0	1.136108432302469	0.0
9	5.284225266523111E-4	0.0	0.0	1.207181262137205	0.0
10	5.284225266523111E-4	0.0	0.0	1.492793637792779	0.0
11	5.284225266523111E-4	0.0	0.0	1.8079976749408828	0.0
12	5.284225266523111E-4	0.0	0.0	2.09545952943974	0.0
13	5.284225266523111E-4	0.0	0.0	2.180799767494088	0.0
14	5.284225266523111E-4	0.0	0.0	2.2167324993064454	0.0
15	5.284225266523111E-4	0.0	0.0	2.3002232585175104	0.0
16	5.284225266523111E-4	0.0	0.0	2.4341783690238716	0.0
17	5.284225266523111E-4	0.0	0.0	2.6220325772487683	0.0
18	5.284225266523111E-4	0.0	0.0	2.813321531896905	0.0
19	5.284225266523111E-4	0.0	0.0	2.9676209096793795	0.0
20	5.284225266523111E-4	0.0	0.0	3.098933907552479	0.0
21	5.284225266523111E-4	0.0	0.0	3.2730491300844156	0.0
22	5.284225266523111E-4	0.0	0.0	3.4585254369393765	0.0
23	5.284225266523111E-4	0.0	0.0	3.6278848567314426	0.0
24	5.284225266523111E-4	0.0	0.0	3.7702947276642402	0.0
25	5.284225266523111E-4	0.0	0.0	3.9095340634371243	0.0
26	5.284225266523111E-4	0.0	0.0	4.041903906363529	0.0
27	5.284225266523111E-4	0.0	0.0	4.1644979325468645	0.0
28	5.284225266523111E-4	0.0	0.0	4.30981412737625	0.0
29	7.926337899784668E-4	0.0	0.0	4.455922955995614	0.0
30	0.0010568450533046223	0.0	0.0	4.609693911251437	0.0
31	0.0010568450533046223	0.0	0.0	4.772712260723675	0.0
32	0.0010568450533046223	0.0	0.0	4.9383727228291745	0.0
33	0.0010568450533046223	0.0	0.0	5.107203720094588	0.0
34	0.0010568450533046223	0.0	0.0	5.278412618729936	0.0
35	0.0010568450533046223	0.0	0.0	5.443280447045457	0.0
36	0.0010568450533046223	0.0	0.0	5.620830416000634	0.0
37	0.0010568450533046223	0.0	0.0	5.7957382723225495	0.0
38	0.0010568450533046223	0.0	0.0	5.989669339603947	0.0
39	0.0010568450533046223	0.0	0.0	6.1923193785751085	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAAACG	20	7.027283E-4	45.000004	44
CTACGAA	40	6.7939254E-9	45.000004	11
GATATCG	20	7.027283E-4	45.000004	44
TATAGAG	20	7.027283E-4	45.000004	1
CAATGCG	20	7.027283E-4	45.000004	1
TAGTGCC	20	7.027283E-4	45.000004	13
GCGAGAC	25	3.8858307E-5	45.0	21
TACGAAT	45	3.8380676E-10	45.0	12
TCTCGTA	25	3.8858307E-5	45.0	28
GGTATAC	25	3.8858307E-5	45.0	8
TCGTACG	25	3.8858307E-5	45.0	30
AAATGCG	25	3.8858307E-5	45.0	1
TAGACGG	60	0.0	44.999996	2
CGTTTTT	7920	0.0	43.52273	1
CGTTATT	495	0.0	42.727276	1
GACGGGC	55	6.002665E-11	40.909092	4
GCGTAAG	50	1.0768417E-9	40.5	1
ACGGGCC	45	1.9224899E-8	40.0	5
ACCTGTA	40	3.4500226E-7	39.375004	14
ATGTGCG	35	6.237191E-6	38.571426	1
>>END_MODULE