##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550397_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	345660
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.180324596424235	33.0	31.0	34.0	30.0	34.0
2	32.285598565063935	34.0	31.0	34.0	30.0	34.0
3	32.054929699704914	33.0	31.0	34.0	30.0	34.0
4	35.885850257478445	37.0	35.0	37.0	35.0	37.0
5	33.763539316090956	37.0	35.0	37.0	32.0	37.0
6	34.783287045073195	37.0	35.0	37.0	32.0	37.0
7	35.85314760168952	37.0	35.0	37.0	33.0	37.0
8	35.880787479025635	37.0	35.0	37.0	35.0	37.0
9	37.87244691315165	39.0	38.0	39.0	35.0	39.0
10	37.58323207776428	39.0	37.0	39.0	35.0	39.0
11	37.53159173754557	39.0	37.0	39.0	35.0	39.0
12	37.48503442689348	39.0	37.0	39.0	35.0	39.0
13	37.4627784528149	39.0	37.0	39.0	35.0	39.0
14	38.69873575189492	40.0	38.0	41.0	35.0	41.0
15	38.844517734189665	40.0	38.0	41.0	35.0	41.0
16	38.789330556037726	40.0	38.0	41.0	35.0	41.0
17	38.7667766012845	40.0	38.0	41.0	35.0	41.0
18	38.3903488977608	39.0	38.0	41.0	35.0	41.0
19	37.945981600416594	39.0	37.0	41.0	34.0	41.0
20	37.61633686281317	39.0	35.0	41.0	34.0	41.0
21	37.43771046693283	39.0	35.0	41.0	34.0	41.0
22	37.44862292426083	39.0	35.0	41.0	34.0	41.0
23	37.466999363536424	39.0	35.0	41.0	34.0	41.0
24	37.3533038245675	39.0	35.0	41.0	34.0	41.0
25	37.15425562691662	38.0	35.0	41.0	33.0	41.0
26	37.18391482960134	39.0	35.0	41.0	33.0	41.0
27	37.170262685876295	39.0	35.0	41.0	33.0	41.0
28	37.01335994908291	38.0	35.0	41.0	33.0	41.0
29	36.904689579355434	38.0	35.0	41.0	33.0	41.0
30	36.556361742753	38.0	35.0	41.0	33.0	41.0
31	36.233842504194875	38.0	35.0	41.0	31.0	41.0
32	35.68287045073193	38.0	35.0	41.0	28.0	41.0
33	35.019548110860384	38.0	35.0	41.0	21.0	41.0
34	34.441465602036686	38.0	35.0	41.0	18.0	41.0
35	34.03243939130938	38.0	34.0	41.0	15.0	41.0
36	33.754730081583055	38.0	34.0	41.0	12.0	41.0
37	33.603480298559276	38.0	34.0	41.0	10.0	41.0
38	33.43793901521727	38.0	33.0	41.0	10.0	41.0
39	33.274058323207775	38.0	33.0	41.0	10.0	41.0
40	33.15560955852572	38.0	33.0	41.0	10.0	41.0
41	32.994853324075684	38.0	33.0	41.0	10.0	41.0
42	32.90039055719493	38.0	33.0	41.0	10.0	41.0
43	32.790279465370595	38.0	33.0	41.0	10.0	41.0
44	32.604903662558584	37.0	32.0	40.0	10.0	41.0
45	32.557814036914884	37.0	31.0	40.0	10.0	41.0
46	32.4170919400567	37.0	31.0	40.0	9.0	41.0
47	32.392651738702774	37.0	31.0	40.0	9.0	41.0
48	32.32795810912457	37.0	31.0	40.0	9.0	41.0
49	32.24410692588092	37.0	31.0	40.0	8.0	41.0
50	32.17413643464676	36.0	31.0	40.0	8.0	41.0
51	31.34420528843372	35.0	28.0	40.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	8.0
10	6.0
11	7.0
12	11.0
13	11.0
14	19.0
15	21.0
16	37.0
17	93.0
18	167.0
19	339.0
20	591.0
21	878.0
22	1443.0
23	2342.0
24	3997.0
25	7235.0
26	11306.0
27	13164.0
28	11691.0
29	9739.0
30	8744.0
31	8568.0
32	8976.0
33	11055.0
34	18318.0
35	26422.0
36	22809.0
37	29223.0
38	51190.0
39	97170.0
40	76.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.915755366545163	17.689637215761152	19.755829427761384	30.638777989932304
2	45.89770294509055	20.805126424810506	20.23317711045536	13.06399351964358
3	26.301568014812243	18.654747439680612	42.34102875658161	12.702655788925535
4	24.012613550888158	19.96470520164323	41.534745125267605	14.487936122201006
5	18.973557831395013	30.591911126540534	37.432448070358156	13.0020829717063
6	21.542556269166234	28.778279233929293	39.11618353295145	10.562980963953017
7	62.29994792570734	6.281895504252734	28.076144187930336	3.3420123821095875
8	63.635942833998726	5.746687496383729	27.697737661285654	2.9196320083318867
9	57.700052074292664	8.75889602499566	29.129202106115837	4.411849794595845
10	31.14360932708442	24.44367297344211	34.96094428050686	9.451773418966615
11	25.258635653532373	20.881502054041544	40.423826881907075	13.436035410519006
12	23.118092923682234	20.032401782098017	42.87074003355899	13.978765260660763
13	19.818607880576288	22.663310767806514	42.704391598680786	14.813689752936412
14	15.745819591506105	24.642712492044204	43.2161661748539	16.395301741595787
15	17.020193253486084	22.585488630446104	45.79876178904125	14.595556327026557
16	18.37267835445235	22.22704391598681	45.045420355262394	14.354857374298444
17	17.67256841983452	22.462246137823293	43.48058786090378	16.384597581438406
18	18.23612798703929	23.333333333333332	43.70045709656888	14.730081583058496
19	20.018804605681883	22.95261239368165	42.85338193600648	14.175201064629983
20	19.534802985592776	23.989758722444023	42.75328357345368	13.722154718509518
21	19.272117109298154	23.14470867326274	43.74877046809003	13.834403749349072
22	18.63883585025748	21.878724758433144	43.236706590291035	16.24573280101834
23	18.609905687669965	21.81160678123011	43.642307469767985	15.936180061331944
24	16.614013770757392	23.06746513915408	44.71069837412486	15.607822715963662
25	16.53850604640398	24.178094080888734	44.02592142567841	15.257478447028872
26	16.33917722617601	25.08736909101429	42.333217612682986	16.240236070126716
27	17.869293525429615	23.545391425099808	43.74529884857953	14.840016200891048
28	14.853324075681304	25.185731643811838	44.11618353295145	15.8447607475554
29	16.688074986981427	22.5273390036452	44.16738992073135	16.617196088642018
30	16.20118035063357	24.067870161430307	43.31337152114795	16.417577966788173
31	18.56246022102644	24.17722617601111	40.94456980848232	16.315743794480124
32	18.217323381357403	23.639703755135105	41.07649134988139	17.066481513626105
33	17.55540126135509	25.840421223167276	39.44714459295261	17.157032922525026
34	16.72597349997107	26.65856622114216	38.79303361684892	17.82242666203784
35	18.394665278018863	25.679280217554822	37.30602325985072	18.620031244575593
36	18.45917954058902	28.760342533125037	35.646878435456806	17.133599490829138
37	18.474223225134526	28.103627842388473	36.276977376612855	17.145171555864145
38	17.593010472718856	28.777122027425794	35.47763698431985	18.152230515535496
39	19.59468842214893	27.205056992420296	35.28669791124226	17.91355667418851
40	18.80836660302031	27.96823468147891	35.47329745993172	17.750101255569056
41	17.512873922351442	27.48336515651218	35.05901753167853	19.944743389457848
42	17.70294509055141	27.96129144245791	34.78909911473703	19.54666435225366
43	19.150899728056473	26.275241566857606	35.19354278771047	19.380315917375455
44	18.287045073193312	27.374009141931378	34.58311635711392	19.755829427761384
45	18.736041196551525	27.622808540183996	33.88705664525835	19.754093618006134
46	18.003529479835674	29.550714575015913	33.77567551929641	18.670080425851992
47	18.89949661517098	26.1658855522768	36.473991783833824	18.460626048718392
48	17.439680611005034	26.14823815309842	36.85297691373026	19.55910432216629
49	17.73071804663542	26.61632818376439	37.35665104437887	18.296302725221317
50	17.389052826476885	26.630793265058152	36.645836949603655	19.334316958861308
51	18.59399409824683	25.62286640050917	34.864606839090435	20.91853266215356
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	418.0
1	1012.0
2	1606.0
3	14215.0
4	26824.0
5	17640.5
6	8457.0
7	7866.5
8	7276.0
9	6997.0
10	6718.0
11	6568.0
12	6418.0
13	6121.5
14	5825.0
15	5447.0
16	5069.0
17	4652.5
18	4236.0
19	4139.5
20	4043.0
21	3742.5
22	3442.0
23	3397.0
24	3352.0
25	3874.5
26	4198.5
27	4000.0
28	4919.5
29	5839.0
30	7048.5
31	8258.0
32	8836.0
33	9414.0
34	10354.0
35	11294.0
36	11398.5
37	11503.0
38	12064.0
39	12625.0
40	13890.5
41	15156.0
42	16641.5
43	18127.0
44	18731.0
45	19335.0
46	20322.5
47	21310.0
48	21187.0
49	21064.0
50	20231.5
51	19399.0
52	17863.0
53	16327.0
54	14910.5
55	13494.0
56	12080.0
57	10666.0
58	10450.5
59	10235.0
60	9304.5
61	8374.0
62	7578.0
63	6782.0
64	5962.0
65	5142.0
66	4271.5
67	3401.0
68	2906.0
69	2411.0
70	1868.5
71	1326.0
72	1152.5
73	979.0
74	761.5
75	410.5
76	277.0
77	202.5
78	128.0
79	115.5
80	103.0
81	67.0
82	31.0
83	22.0
84	13.0
85	9.5
86	6.0
87	10.5
88	15.0
89	7.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	345660.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.52924433183927
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.60339839971466	28.282485913882056
2	7.30342603764706	5.189704163021583
3	2.6407095750390135	2.8146724710296565
4	1.5669327836340945	2.226877508852191
5	1.0955560830135216	1.9462139876310096
6	0.8397285858203247	1.7900953258824108
7	0.6211430701143417	1.544812073218485
8	0.5311109519422463	1.5095976623097453
9	0.4623671070160856	1.4784798524562164
>10	4.947730578561021	36.73835721454998
>50	0.33715926778277383	7.465077294842687
>100	0.04582747329086246	3.068604703788499
>500	0.0024550432120104895	0.6998419103675306
>1k	0.0016366954746736594	0.6396560875814571
>5k	0.0	0.0
>10k+	8.183477373368297E-4	4.605523830586491
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15840	4.5825377538621765	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1118	0.3234392177284036	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1082	0.3130243591968987	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	983	0.28438349823526005	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	717	0.20742926575247353	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	707	0.20453624949372218	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	390	0.1128276340913036	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	385	0.11138112596192791	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	383	0.11080252271017763	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	371	0.10733090319967598	No Hit
GCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC	349	0.10096626743042296	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.893016258751374E-4	0.0	0.0	0.061042643059654	0.0
2	2.893016258751374E-4	0.0	0.0	0.3269108372389053	0.0
3	2.893016258751374E-4	0.0	0.0	0.43858126482670834	0.0
4	2.893016258751374E-4	0.0	0.0	0.5780246484985245	0.0
5	2.893016258751374E-4	0.0	0.0	0.9798646068390905	0.0
6	2.893016258751374E-4	0.0	0.0	1.189608285598565	0.0
7	2.893016258751374E-4	0.0	0.0	1.4164207602846728	0.0
8	2.893016258751374E-4	0.0	0.0	1.7415957877683272	0.0
9	2.893016258751374E-4	0.0	0.0	1.8625238673841347	0.0
10	2.893016258751374E-4	0.0	0.0	2.220679280217555	0.0
11	2.893016258751374E-4	0.0	0.0	2.730718046635422	0.0
12	2.893016258751374E-4	0.0	0.0	3.141237053752242	0.0
13	2.893016258751374E-4	0.0	0.0	3.29427761384019	0.0
14	2.893016258751374E-4	0.0	0.0	3.3399872707284617	0.0
15	2.893016258751374E-4	0.0	0.0	3.4661227796100214	0.0
16	2.893016258751374E-4	0.0	0.0	3.7111612567262626	0.0
17	2.893016258751374E-4	0.0	0.0	4.0065382167447785	0.0
18	2.893016258751374E-4	0.0	0.0	4.331134640976682	0.0
19	2.893016258751374E-4	0.0	0.0	4.552450384771163	0.0
20	2.893016258751374E-4	0.0	0.0	4.775791239946768	0.0
21	2.893016258751374E-4	0.0	0.0	5.019093907307759	0.0
22	2.893016258751374E-4	0.0	0.0	5.3182317884626515	0.0
23	2.893016258751374E-4	0.0	0.0	5.621419892379795	0.0
24	2.893016258751374E-4	0.0	0.0	5.869640687380663	0.0
25	2.893016258751374E-4	0.0	0.0	6.059422553954753	0.0
26	2.893016258751374E-4	0.0	0.0	6.257594167679223	0.0
27	2.893016258751374E-4	0.0	0.0	6.467916449690447	0.0
28	2.893016258751374E-4	0.0	0.0	6.692993114621304	0.0
29	2.893016258751374E-4	0.0	0.0	6.931088352716542	0.0
30	2.893016258751374E-4	0.0	0.0	7.189145402997164	0.0
31	5.786032517502748E-4	0.0	0.0	7.418850893942024	0.0
32	5.786032517502748E-4	0.0	0.0	7.656367528785512	0.0
33	5.786032517502748E-4	0.0	0.0	7.884626511600995	0.0
34	5.786032517502748E-4	0.0	0.0	8.120407336689231	0.0
35	5.786032517502748E-4	0.0	0.0	8.368917433315975	0.0
36	5.786032517502748E-4	0.0	0.0	8.637678643753977	0.0
37	8.679048776254123E-4	0.0	0.0	8.876641786726841	0.0
38	8.679048776254123E-4	0.0	0.0	9.123126771972458	0.0
39	8.679048776254123E-4	0.0	0.0	9.380894520627207	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTC	30	2.1610813E-6	45.000004	5
GATGGTC	30	2.1610813E-6	45.000004	24
GACGGGT	30	2.1610813E-6	45.000004	4
CGTAAAT	30	2.1610813E-6	45.000004	15
CGGCGTT	20	7.0264353E-4	45.0	42
ATCTCGT	25	3.885127E-5	45.0	19
TACCCTG	25	3.885127E-5	45.0	28
ATTAGCG	20	7.0264353E-4	45.0	1
CGGCATC	20	7.0264353E-4	45.0	22
CTATAAC	20	7.0264353E-4	45.0	21
TAGCGGC	20	7.0264353E-4	45.0	33
ATTACCG	20	7.0264353E-4	45.0	1
GGCCGAT	35	1.2086275E-7	45.0	8
CACGACA	20	7.0264353E-4	45.0	14
TACGGCT	25	3.885127E-5	45.0	7
CGACAAA	20	7.0264353E-4	45.0	16
ACGGGCG	20	7.0264353E-4	45.0	5
CATTATC	20	7.0264353E-4	45.0	35
TCGGCAT	20	7.0264353E-4	45.0	21
TCGTGTA	85	0.0	45.0	17
>>END_MODULE