##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550395_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	452530
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17687888095817	33.0	31.0	34.0	30.0	34.0
2	32.27109362915166	34.0	31.0	34.0	30.0	34.0
3	32.08274368550151	33.0	31.0	34.0	30.0	34.0
4	35.87554195301969	37.0	35.0	37.0	35.0	37.0
5	33.89398271937772	37.0	35.0	37.0	32.0	37.0
6	34.8334386670497	37.0	35.0	37.0	32.0	37.0
7	35.85839391863523	37.0	35.0	37.0	33.0	37.0
8	35.88083000022098	37.0	35.0	37.0	35.0	37.0
9	37.85724482354761	39.0	38.0	39.0	35.0	39.0
10	37.51330077563919	39.0	37.0	39.0	35.0	39.0
11	37.42465913862064	39.0	37.0	39.0	35.0	39.0
12	37.355689125582835	39.0	37.0	39.0	35.0	39.0
13	37.311597021191965	39.0	37.0	39.0	34.0	39.0
14	38.36916226548516	40.0	38.0	41.0	33.0	41.0
15	38.579075420414114	40.0	38.0	41.0	34.0	41.0
16	38.57864892935275	40.0	38.0	41.0	34.0	41.0
17	38.59037632864119	40.0	38.0	41.0	34.0	41.0
18	38.303796433385635	39.0	38.0	41.0	34.0	41.0
19	37.9508562968201	39.0	37.0	41.0	34.0	41.0
20	37.65037235100435	39.0	35.0	41.0	34.0	41.0
21	37.489178617992174	39.0	35.0	41.0	33.0	41.0
22	37.53377013678651	39.0	35.0	41.0	34.0	41.0
23	37.55886018606501	39.0	35.0	41.0	34.0	41.0
24	37.446149426557355	39.0	35.0	41.0	34.0	41.0
25	37.24015866351402	39.0	35.0	41.0	33.0	41.0
26	37.27182286257265	39.0	35.0	41.0	33.0	41.0
27	37.24134311537357	39.0	35.0	41.0	33.0	41.0
28	37.1163569266126	39.0	35.0	41.0	33.0	41.0
29	37.019680463173714	39.0	35.0	41.0	33.0	41.0
30	36.69419486000928	39.0	35.0	41.0	33.0	41.0
31	36.391397255430576	39.0	35.0	41.0	31.0	41.0
32	35.96825624820454	39.0	35.0	41.0	30.0	41.0
33	35.44411641217157	39.0	35.0	41.0	24.0	41.0
34	34.954358827039094	39.0	35.0	41.0	21.0	41.0
35	34.61519015313902	39.0	35.0	41.0	18.0	41.0
36	34.35781716129318	38.0	34.0	41.0	16.0	41.0
37	34.23321768722516	38.0	34.0	41.0	15.0	41.0
38	34.07785782158089	38.0	34.0	41.0	15.0	41.0
39	33.95608025987227	38.0	34.0	41.0	15.0	41.0
40	33.82521379798025	38.0	34.0	41.0	13.0	41.0
41	33.625198329392525	38.0	33.0	41.0	12.0	41.0
42	33.57914171436148	38.0	33.0	41.0	10.0	41.0
43	33.492745232360285	38.0	33.0	41.0	10.0	41.0
44	33.333562415751445	38.0	33.0	41.0	10.0	41.0
45	33.28937087043953	38.0	33.0	40.0	10.0	41.0
46	33.21564537157757	37.0	33.0	40.0	10.0	41.0
47	33.18599429872053	37.0	33.0	40.0	10.0	41.0
48	33.100095021324556	37.0	33.0	40.0	10.0	41.0
49	33.02108368505955	37.0	33.0	40.0	10.0	41.0
50	32.956725520959935	37.0	33.0	40.0	10.0	41.0
51	32.0765253132389	35.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	12.0
10	13.0
11	16.0
12	8.0
13	15.0
14	18.0
15	24.0
16	54.0
17	131.0
18	232.0
19	394.0
20	677.0
21	1104.0
22	1671.0
23	2739.0
24	4650.0
25	8216.0
26	12366.0
27	14481.0
28	13498.0
29	11916.0
30	10734.0
31	11028.0
32	12276.0
33	16097.0
34	26489.0
35	32712.0
36	29573.0
37	39844.0
38	70709.0
39	130741.0
40	84.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.24502242945219	18.3996641106667	20.0415442070139	27.313769252867214
2	43.604843877753964	20.911320796411285	21.297151570061654	14.186683755773096
3	27.554637261618016	19.29485337988642	39.59207124389543	13.558438114600138
4	25.67409895476543	21.00457428236802	38.525622610655645	14.795704152210904
5	20.395774865754756	29.352971073740967	36.055510131924954	14.195743928579322
6	23.017479504121276	29.36932358075708	36.10125295560515	11.511943959516497
7	66.2455527810311	6.074293417011027	24.41031533820962	3.26983846374826
8	67.71749939230548	5.523832674076857	23.76969482686231	2.988973106755353
9	61.99345899719355	8.094049013325083	25.403840629350537	4.50865136013082
10	35.37113561531832	22.347468676109873	31.70927894283252	10.572116765739288
11	27.07378516341458	21.70375444721897	37.53497005723377	13.687490332132676
12	25.573111174949727	19.591408304421805	40.167723686827394	14.667756833801073
13	22.037433982277417	20.878836762203612	40.96258811570503	16.121141139813936
14	16.76220361081033	22.8740635979935	42.9655492453539	17.398183545842265
15	17.911519678253377	22.972620599739244	43.698539323359775	15.417320398647604
16	21.130974742006057	22.056880206837114	41.613152719156744	15.198992332000088
17	20.869555609572846	22.74125472344375	40.04728968245199	16.341899984531413
18	20.485271694694273	22.716284003270502	41.346651050759064	15.451793251276158
19	21.63591364108457	22.748326077829095	39.55737741144233	16.058382869644003
20	20.742713190285727	24.22756502331337	40.64349324906636	14.386228537334542
21	20.217665127173888	22.801140255894637	42.25487812962677	14.726316487304707
22	19.61129648863059	21.471283671800766	42.3313371489183	16.586082690650343
23	19.613285307051466	22.038096921751045	42.349236514706206	15.999381256491283
24	18.024440368594348	23.593573906702318	41.78684286124677	16.595142863456566
25	18.985039665878507	24.209223697876382	40.68437451660664	16.121362119638476
26	18.25315448699534	24.56455925573995	39.329105252690425	17.85318100457428
27	17.849866307206153	23.234260711996995	42.18659536384328	16.72927761695357
28	16.590723266965725	24.714162596954896	41.37515744812499	17.31995668795439
29	17.963007977371667	24.128345082094004	40.84480586922414	17.06384107131019
30	18.69467217643029	23.958632576845734	40.03535677192672	17.31133847479725
31	19.904094756148762	24.118621969814154	39.17110467814289	16.806178595894195
32	20.125958499988954	24.71239475835856	38.17757938700196	16.98406735465052
33	19.961991470178774	25.065520517976708	37.181844297615626	17.79064371422889
34	18.368947915055355	25.935739067023185	37.79881996773695	17.896493050184517
35	20.025854639471415	26.202461715245395	35.29710737409674	18.47457627118644
36	21.238812896382562	27.599717145824588	33.640863589154314	17.520606368638543
37	19.728194815813318	28.824166353611915	34.15795637858264	17.28968245199213
38	19.75824807195103	27.40525490022761	33.99531522771971	18.84118180010165
39	20.55289152100413	26.504099175745253	33.47115108390604	19.471858219344572
40	20.63045543941838	26.779219057300068	34.238614014540474	18.351711488741078
41	18.450268490486817	27.672861467747996	34.340927673303426	19.535942368461757
42	18.712129582569112	27.461604755485823	34.298499546991366	19.527766114953703
43	19.676706516695024	26.735465052040748	34.50511568293815	19.082712748326077
44	20.356661436810818	27.13499657481272	32.858815990100105	19.649525998276356
45	19.455284732503923	27.23620533445296	32.606898990122204	20.70161094292091
46	18.796543875544163	27.893841292290013	33.74538704616268	19.564227786003137
47	19.314078624621573	26.501005458201664	35.884692727553976	18.300223189622788
48	18.814001281682984	27.21101363445517	35.02419729078735	18.950787793074493
49	19.032771307979583	27.15377985989879	34.97182507237089	18.841623759750732
50	18.097363710693216	26.887941130974742	35.02662806885731	19.988067089474733
51	19.363578105318986	25.791881201246326	34.04017413210174	20.80436656133295
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	361.0
1	1152.5
2	1944.0
3	15488.5
4	29033.0
5	19085.5
6	9138.0
7	8546.5
8	7955.0
9	7491.5
10	7028.0
11	6841.0
12	6654.0
13	6314.0
14	5974.0
15	5566.5
16	5159.0
17	4894.0
18	4629.0
19	4197.5
20	3766.0
21	3649.0
22	3532.0
23	3732.5
24	3933.0
25	4547.5
26	5333.0
27	5504.0
28	7356.0
29	9208.0
30	9826.0
31	10444.0
32	11667.0
33	12890.0
34	13896.0
35	14902.0
36	15581.5
37	16261.0
38	17008.0
39	17755.0
40	18705.5
41	19656.0
42	20914.0
43	22172.0
44	23117.0
45	24062.0
46	25771.0
47	27480.0
48	27585.0
49	27690.0
50	26970.0
51	26250.0
52	24624.0
53	22998.0
54	21209.0
55	19420.0
56	18400.0
57	17380.0
58	16630.5
59	15881.0
60	14943.0
61	14005.0
62	12336.5
63	10668.0
64	9235.5
65	7803.0
66	6943.5
67	6084.0
68	5185.0
69	4286.0
70	3307.5
71	2329.0
72	1832.0
73	1335.0
74	1123.5
75	693.0
76	474.0
77	330.5
78	187.0
79	159.0
80	131.0
81	99.0
82	67.0
83	39.5
84	12.0
85	10.0
86	8.0
87	5.5
88	3.0
89	2.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	452530.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.84109804319556
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.37938474532965	26.186033456888513
2	7.590700823826108	5.1375530159132925
3	2.9098728487385874	2.954198771021859
4	1.609161767162422	2.178232045196214
5	1.0952049286496195	1.8531468683911385
6	0.9105633187690633	1.848867752700084
7	0.7137835756193117	1.6908653974909074
8	0.6183417091153293	1.6740289921895173
9	0.5747163812641717	1.7504130064852252
>10	6.354599214523142	43.15776006040629
>50	0.19126838872249516	3.973193618539435
>100	0.04847212590912548	2.9934170176722006
>500	0.002620114914006783	0.5712407919535887
>1k	6.550287285016957E-4	0.22588062359359984
>5k	0.0	0.0
>10k+	6.550287285016957E-4	3.805168581558131
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17166	3.793339668088304	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1019	0.22517844120831768	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	931	0.20573221664861996	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	567	0.12529556051532495	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	564	0.12463262104169891	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	515	0.11380460963913994	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	453	0.10010386051753474	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.839192981680773E-4	0.0	0.0	0.03336795350584491	0.0
2	8.839192981680773E-4	0.0	0.0	0.21766512717388903	0.0
3	8.839192981680773E-4	0.0	0.0	0.29589198506176384	0.0
4	8.839192981680773E-4	0.0	0.0	0.4026252403155592	0.0
5	8.839192981680773E-4	0.0	0.0	0.686584314852054	0.0
6	8.839192981680773E-4	0.0	0.0	0.858064658696661	0.0
7	8.839192981680773E-4	0.0	0.0	1.029324022716726	0.0
8	8.839192981680773E-4	0.0	0.0	1.2858815990100103	0.0
9	8.839192981680773E-4	0.0	0.0	1.3864274191766293	0.0
10	8.839192981680773E-4	0.0	0.0	1.646078713013502	0.0
11	8.839192981680773E-4	0.0	0.0	2.124279053322432	0.0
12	8.839192981680773E-4	0.0	0.0	2.4864649857468013	0.0
13	8.839192981680773E-4	0.0	0.0	2.6179479813493027	0.0
14	8.839192981680773E-4	0.0	0.0	2.67672861467748	0.0
15	8.839192981680773E-4	0.0	0.0	2.7867765672994054	0.0
16	8.839192981680773E-4	0.0	0.0	2.986984288334475	0.0
17	8.839192981680773E-4	0.0	0.0	3.211278810244625	0.0
18	8.839192981680773E-4	0.0	0.0	3.465847568117031	0.0
19	8.839192981680773E-4	0.0	0.0	3.639979669856142	0.0
20	8.839192981680773E-4	0.0	0.0	3.8496895233465183	0.0
21	8.839192981680773E-4	0.0	0.0	4.083707157536517	0.0
22	8.839192981680773E-4	0.0	0.0	4.360373897863125	0.0
23	8.839192981680773E-4	0.0	0.0	4.579585883808808	0.0
24	8.839192981680773E-4	0.0	0.0	4.772943230283075	0.0
25	8.839192981680773E-4	2.2097982454201932E-4	0.0	4.9581243232492875	0.0
26	8.839192981680773E-4	2.2097982454201932E-4	0.0	5.1300466267429785	0.0
27	8.839192981680773E-4	2.2097982454201932E-4	0.0	5.383289505668133	0.0
28	0.0011048991227100965	2.2097982454201932E-4	0.0	5.587032903895874	0.0
29	0.0011048991227100965	2.2097982454201932E-4	0.0	5.821271517910414	0.0
30	0.0011048991227100965	2.2097982454201932E-4	0.0	6.073851457361942	0.0
31	0.0011048991227100965	2.2097982454201932E-4	0.0	6.2647780257662475	0.0
32	0.0011048991227100965	2.2097982454201932E-4	0.0	6.4806753143438005	0.0
33	0.0011048991227100965	2.2097982454201932E-4	0.0	6.683755773097916	0.0
34	0.0013258789472521159	2.2097982454201932E-4	0.0	6.917994387112457	0.0
35	0.0013258789472521159	2.2097982454201932E-4	0.0	7.153558880074249	0.0
36	0.0013258789472521159	2.2097982454201932E-4	0.0	7.3550924800565705	0.0
37	0.0013258789472521159	2.2097982454201932E-4	0.0	7.568558990564162	0.0
38	0.0013258789472521159	2.2097982454201932E-4	0.0	7.833734780014584	0.0
39	0.0013258789472521159	2.2097982454201932E-4	0.0	8.332928203655007	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	35	1.2097371E-7	45.000004	21
ACTACGG	35	1.2097371E-7	45.000004	2
CGAATAT	60	0.0	45.000004	14
TCACGAC	20	7.028749E-4	45.0	25
ATTACCG	20	7.028749E-4	45.0	1
ATAGCGG	40	6.7993824E-9	45.0	2
CGACCGT	20	7.028749E-4	45.0	22
GTTAGCG	25	3.8870443E-5	45.0	1
ATCGTTC	20	7.028749E-4	45.0	10
ATCCGGG	20	7.028749E-4	45.0	3
GCCGATT	20	7.028749E-4	45.0	9
TTGCGCG	20	7.028749E-4	45.0	1
TTCGGGC	25	3.8870443E-5	45.0	4
CGGTCTA	20	7.028749E-4	45.0	31
ATTGCGG	25	3.8870443E-5	45.0	2
TTACCGG	20	7.028749E-4	45.0	2
AGGGCGC	20	7.028749E-4	45.0	6
ACGGTTC	20	7.028749E-4	45.0	34
TACCGGA	25	3.8870443E-5	45.0	31
CGTTATT	450	0.0	44.0	1
>>END_MODULE