FastQCFastQC Report
Sat 18 Jun 2016
SRR3550392_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550392_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366390
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163684.467370834356832No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG10750.2934032042359235No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG10100.2756625453751467No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9890.26993094789704963No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8940.24400229263899123No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA5220.1424711373126996No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC4280.11681541526788394No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTCG302.1614378E-645.00000432
CGTTAGG453.8380676E-1045.0000042
ATGAGTC302.1614378E-645.00000413
TACTCGT302.1614378E-645.00000433
CAATAGA302.1614378E-645.00000434
CGGGTAC253.8855855E-545.06
GCGGTAG207.026989E-445.01
GGATCGC253.8855855E-545.08
TGCGGGT253.8855855E-545.04
GTTACGG253.8855855E-545.02
TACGCGG207.026989E-445.02
GCGATCA351.2089004E-745.09
GGTTATG502.1827873E-1145.01
TTCGAAG253.8855855E-545.02
TCAACGG253.8855855E-545.02
ATACACC207.026989E-445.020
CGTTATT4300.043.9534871
CGTTTTT74600.043.582441
GCGATGA750.042.0000049
CGTACAT603.6379788E-1241.25000435