##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550391_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	177388
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21433806119918	33.0	31.0	34.0	30.0	34.0
2	32.32646514984103	34.0	31.0	34.0	31.0	34.0
3	32.07931201659639	33.0	31.0	34.0	30.0	34.0
4	35.91489277741448	37.0	35.0	37.0	35.0	37.0
5	33.96370103952917	37.0	35.0	37.0	32.0	37.0
6	34.87600626874422	37.0	35.0	37.0	32.0	37.0
7	35.856004915777845	37.0	35.0	37.0	33.0	37.0
8	35.8427740320653	37.0	35.0	37.0	35.0	37.0
9	37.807331950301034	39.0	38.0	39.0	35.0	39.0
10	37.53684578438226	39.0	37.0	39.0	35.0	39.0
11	37.490258642072746	39.0	37.0	39.0	35.0	39.0
12	37.44116851196248	39.0	37.0	39.0	35.0	39.0
13	37.415473425485374	39.0	37.0	39.0	35.0	39.0
14	38.66638667779106	40.0	38.0	41.0	35.0	41.0
15	38.800905360001806	40.0	38.0	41.0	35.0	41.0
16	38.74634135341737	40.0	38.0	41.0	35.0	41.0
17	38.71770920242632	40.0	38.0	41.0	35.0	41.0
18	38.34293751550274	39.0	38.0	41.0	35.0	41.0
19	37.91844431415879	39.0	37.0	41.0	34.0	41.0
20	37.56973414210657	39.0	35.0	41.0	34.0	41.0
21	37.40254695920807	39.0	35.0	41.0	34.0	41.0
22	37.38776016415992	39.0	35.0	41.0	34.0	41.0
23	37.39398944686224	39.0	35.0	41.0	34.0	41.0
24	37.29456896746116	39.0	35.0	41.0	34.0	41.0
25	37.08712539743387	38.0	35.0	41.0	33.0	41.0
26	37.10511421291181	38.0	35.0	41.0	33.0	41.0
27	37.09902586420728	38.0	35.0	41.0	33.0	41.0
28	36.97274336482739	38.0	35.0	41.0	33.0	41.0
29	36.87362166550161	38.0	35.0	41.0	33.0	41.0
30	36.53544771912418	38.0	35.0	41.0	33.0	41.0
31	36.204292285836694	38.0	35.0	41.0	31.0	41.0
32	35.68259408753693	38.0	35.0	41.0	28.0	41.0
33	35.06853902180531	38.0	35.0	41.0	21.0	41.0
34	34.50739621620403	38.0	35.0	41.0	18.0	41.0
35	34.13350959478657	38.0	34.0	41.0	15.0	41.0
36	33.848467765576025	38.0	34.0	41.0	15.0	41.0
37	33.68441495478837	38.0	34.0	41.0	12.0	41.0
38	33.504521162649105	38.0	33.0	41.0	10.0	41.0
39	33.36970933772296	38.0	33.0	41.0	10.0	41.0
40	33.248709044580245	38.0	33.0	41.0	10.0	41.0
41	33.053650754278756	38.0	33.0	41.0	10.0	41.0
42	32.96598980765328	37.0	33.0	41.0	10.0	41.0
43	32.88452431957066	37.0	33.0	41.0	10.0	41.0
44	32.710070579746095	37.0	32.0	40.0	10.0	41.0
45	32.68962951270661	37.0	32.0	40.0	10.0	41.0
46	32.60990596883668	37.0	32.0	40.0	10.0	41.0
47	32.58683789207838	37.0	31.0	40.0	10.0	41.0
48	32.52754414052811	36.0	31.0	40.0	10.0	41.0
49	32.425744695244326	36.0	31.0	40.0	10.0	41.0
50	32.365667350666335	36.0	31.0	40.0	10.0	41.0
51	31.502063273727646	35.0	29.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	8.0
11	4.0
12	11.0
13	9.0
14	17.0
15	12.0
16	20.0
17	64.0
18	110.0
19	159.0
20	280.0
21	420.0
22	664.0
23	1068.0
24	1851.0
25	3372.0
26	5293.0
27	6656.0
28	6278.0
29	5169.0
30	4589.0
31	4511.0
32	4690.0
33	5854.0
34	10184.0
35	14019.0
36	11773.0
37	15032.0
38	25566.0
39	49661.0
40	37.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.30432723746815	17.24017408167407	20.001916702369947	30.453581978487836
2	47.09055854962004	20.792838297968295	18.92912711119129	13.187476041220375
3	26.04009290369134	19.709337722957585	41.20797348185898	13.042595891492095
4	24.04503123097391	20.691365819559383	40.7366901932487	14.526912756218008
5	20.00868153426387	29.12598371930458	37.21334024849482	13.651994497936727
6	21.49807202291023	29.326673732157754	38.35828804654204	10.81696619838997
7	62.01997880352673	6.24112115813922	28.16988747829616	3.5690125600378835
8	62.369495118046316	6.839245044760638	27.788238212280426	3.0030216249126207
9	56.83417142084019	8.931269307957697	29.24662322141295	4.9879360497891625
10	32.81732698942431	21.64351590862967	35.14048300899722	10.39867409294879
11	25.512436015965	20.594966965070917	40.14025751460076	13.752339504363317
12	23.65661713306424	19.774167361941057	41.88445667125172	14.68475883374298
13	19.67945971542607	21.821656481836428	42.57108710848536	15.927796694252146
14	16.005592261032316	23.715809412136107	43.381175727783166	16.897422599048415
15	16.453198638013845	22.276591426703046	45.729699866958306	15.540510068324803
16	18.62189099600875	21.454664351590864	44.23185333844454	15.691591313955849
17	17.654520035177125	22.853857081651523	42.451575078359305	17.04004780481205
18	17.806165016799334	22.529708886734166	43.4493877827136	16.214738313752903
19	20.06674634135342	22.07026405393826	41.970144541908134	15.89284506280019
20	20.63273727647868	21.69932577175457	43.92405348727084	13.743883464495907
21	19.57685976503484	22.458678151847927	43.253771393780866	14.71069068933637
22	18.85189528040228	21.480596207184252	43.77128103366631	15.896227478747152
23	19.18449951518705	21.282724874286874	43.67657338715133	15.85620222337475
24	17.437481678580287	21.679031276072788	44.718357498816154	16.165129546530768
25	17.2497575935238	24.29871242699619	42.58969039619365	15.861839583286356
26	17.14772137912373	23.275531603039664	41.994948925519196	17.581798092317406
27	17.06823460437008	23.96047083229982	43.47870205425395	15.49259250907615
28	16.01122962094392	24.10929713396622	43.738584346178996	16.140888898910863
29	16.503934877218303	23.529212799061945	42.77290459332085	17.1939477303989
30	16.81398967235664	24.444156312715627	42.23340924978014	16.508444765147587
31	18.64951405957562	24.19780368457844	40.01905427650123	17.133627979344713
32	17.990506685908855	24.650483685480413	41.098608699573816	16.260400929036916
33	18.266173585586397	25.750896340225943	38.72189776084065	17.261032313347012
34	17.572778316458837	26.241346652535686	38.761923016213046	17.423952014792434
35	19.440435655173967	25.383904209980386	37.47885990033148	17.696800234514175
36	19.2854082576048	28.835659683856857	34.4966965070918	17.382235551446545
37	18.665862403319277	29.218436421854914	35.991724355649765	16.123976819176043
38	18.675445915169007	28.506437865019052	35.03788305860599	17.780233161205945
39	20.437120887545944	26.4544388571944	35.41840485263941	17.690035402620243
40	20.417390127855324	26.942070489548335	34.700768935891944	17.9397704467044
41	17.51978713328974	28.702054253951793	34.82422711795612	18.953931494802355
42	17.562631068617947	28.21216767763321	34.869325997248964	19.35587525649988
43	19.575168557061357	27.264527476492212	34.113356033102576	19.046947933343855
44	18.508580061785466	28.607910343427967	33.92224953209912	18.961260062687444
45	18.917852391368076	27.69916792567705	34.50515254695921	18.87782713599567
46	18.65176900354026	28.925857442442553	34.15845491239543	18.263918641621757
47	19.03285453356484	26.29884772363407	37.208266624574385	17.460031118226713
48	18.108891244052586	25.957223712990736	37.5329785554829	18.400906487473787
49	18.2131824024173	27.501296592779667	36.7741899113807	17.511331093422328
50	17.95780999842154	26.60270142286964	36.851421742169705	18.588066836539113
51	18.007418765643674	26.088010462939998	35.841206846009875	20.063363925406456
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	205.0
1	544.5
2	884.0
3	7721.5
4	14559.0
5	9530.5
6	4502.0
7	4153.0
8	3804.0
9	3602.5
10	3401.0
11	3303.5
12	3206.0
13	3023.0
14	2840.0
15	2625.0
16	2410.0
17	2120.5
18	1831.0
19	1818.0
20	1805.0
21	1711.0
22	1617.0
23	1580.5
24	1544.0
25	1755.0
26	2137.5
27	2309.0
28	2916.0
29	3523.0
30	3892.0
31	4261.0
32	4735.0
33	5209.0
34	5544.5
35	5880.0
36	5854.5
37	5829.0
38	5910.0
39	5991.0
40	6703.0
41	7415.0
42	7716.5
43	8018.0
44	8638.5
45	9259.0
46	9984.0
47	10709.0
48	10337.5
49	9966.0
50	9240.0
51	8514.0
52	8278.0
53	8042.0
54	7283.5
55	6525.0
56	6264.5
57	6004.0
58	5605.5
59	5207.0
60	5404.5
61	5602.0
62	4979.5
63	4357.0
64	3795.0
65	3233.0
66	2868.5
67	2504.0
68	2119.5
69	1735.0
70	1417.0
71	1099.0
72	893.5
73	688.0
74	584.5
75	356.0
76	231.0
77	182.0
78	133.0
79	93.5
80	54.0
81	35.5
82	17.0
83	13.0
84	9.0
85	6.5
86	4.0
87	3.0
88	2.0
89	2.0
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	177388.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.150134169165895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.06546486604498	27.859268947166665
2	6.727380469700278	4.863914131733827
3	2.9660356173782865	3.2166775655624957
4	1.938371331441225	2.8028953480506007
5	1.4767800891993887	2.669289918145534
6	1.1914044225431182	2.5841657834802803
7	0.9637276611670773	2.4387218977608405
8	0.7641206374949319	2.2098450853496288
9	0.8296166921373546	2.699167925677047
>10	5.9305118048841345	38.277673799806074
>50	0.10292237158095001	2.4911493449387785
>100	0.040545176683404544	2.903804090468352
>500	0.0015594298724386363	0.33711412271405056
>1k	0.0	0.0
>5k	0.0015594298724386363	4.646312039145827
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8242	4.646312039145827	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	598	0.33711412271405056	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	492	0.27735810765102487	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	449	0.25311746003111824	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	380	0.21421967664103547	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	275	0.15502739756917042	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	257	0.14488014972827926	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	255	0.143752677745958	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	203	0.11443840620560577	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	202	0.11387467021444517	No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	200	0.11274719823212392	No Hit
ATCCGCTAGTTCTGGCCCCTGGCCTCTGGCATAATATCTTCACAATGGTGC	189	0.10654610232935713	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	188	0.1059823663381965	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	187	0.10541863034703589	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.043971407310528336	0.0
2	0.0	0.0	0.0	0.2649559158454912	0.0
3	0.0	0.0	0.0	0.3517712584842267	0.0
4	0.0	0.0	0.0	0.45211626491081697	0.0
5	0.0	0.0	0.0	0.7852842356867432	0.0
6	0.0	0.0	0.0	0.9250907614945768	0.0
7	0.0	0.0	0.0	1.098157710780887	0.0
8	0.0	0.0	0.0	1.30110266759871	0.0
9	0.0	0.0	0.0	1.3811531783435183	0.0
10	0.0	0.0	0.0	1.5998827429138387	0.0
11	0.0	0.0	0.0	1.9302320337339618	0.0
12	0.0	0.0	0.0	2.239723092881142	0.0
13	0.0	0.0	0.0	2.346269195210499	0.0
14	0.0	0.0	0.0	2.3840395066182607	0.0
15	0.0	0.0	0.0	2.4691636412835143	0.0
16	0.0	0.0	0.0	2.6608338782781247	0.0
17	0.0	0.0	0.0	2.90211288249487	0.0
18	0.0	0.0	0.0	3.127607278959118	0.0
19	0.0	0.0	0.0	3.29334566036034	0.0
20	0.0	0.0	0.0	3.4308972422035313	0.0
21	0.0	0.0	0.0	3.6045279274810023	0.0
22	0.0	0.0	0.0	3.7877421246082035	0.0
23	0.0	0.0	0.0	3.950098090062462	0.0
24	0.0	0.0	0.0	4.096669447764223	0.0
25	0.0	0.0	0.0	4.228019933704648	0.0
26	0.0	0.0	0.0	4.352605587751144	0.0
27	0.0	0.0	0.0	4.477754977788802	0.0
28	0.0	0.0	0.0	4.59444832795905	0.0
29	0.0	0.0	0.0	4.738201005705008	0.0
30	0.0	0.0	0.0	4.885899835389091	0.0
31	0.0	0.0	0.0	5.043182176922904	0.0
32	0.0	0.0	0.0	5.196518366518592	0.0
33	0.0	0.0	0.0	5.344780932193835	0.0
34	0.0	0.0	0.0	5.485151193992829	0.0
35	0.0	0.0	0.0	5.655963199314497	0.0
36	0.0	0.0	0.0	5.809863124901346	0.0
37	0.0	0.0	0.0	5.964890522470516	0.0
38	0.0	0.0	0.0	6.115408032110402	0.0
39	0.0	0.0	0.0	6.283401357476267	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCT	20	7.017142E-4	45.000004	36
ACGTTTT	20	7.017142E-4	45.000004	29
TTTCGAC	20	7.017142E-4	45.000004	24
GGCAACG	20	7.017142E-4	45.000004	1
TACGGGA	20	7.017142E-4	45.000004	4
ACGGGAA	20	7.017142E-4	45.000004	5
CTTGGCG	20	7.017142E-4	45.000004	1
GCTTAGT	20	7.017142E-4	45.000004	25
GCGATGT	20	7.017142E-4	45.000004	9
TCCAAGT	40	6.7593646E-9	45.000004	45
ACCACCG	40	6.7593646E-9	45.000004	14
GGGTTAG	20	7.017142E-4	45.000004	7
CTTTCGA	20	7.017142E-4	45.000004	23
GAATTAC	20	7.017142E-4	45.000004	12
AGGGCCG	20	7.017142E-4	45.000004	6
TCGACGT	20	7.017142E-4	45.000004	26
TTCGACG	20	7.017142E-4	45.000004	25
CGATAGG	20	7.017142E-4	45.000004	2
GATAGTG	20	7.017142E-4	45.000004	40
GATAGCG	20	7.017142E-4	45.000004	1
>>END_MODULE