FastQCFastQC Report
Sat 18 Jun 2016
SRR3550390_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550390_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235988
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123305.22484194111565No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCG9030.38264657524958895No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7690.32586402698442296No Hit
GAATCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTC7240.3067952607759717No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGC6590.27925148736376426No Hit
GCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC3790.16060138651117853TruSeq Adapter, Index 16 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT3580.1517026289472346No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC3220.13644761598047359No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG2740.11610759869145888No Hit
GAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG2570.1089038425682662No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGA302.1581473E-645.00000413
GCGTTGG253.8813538E-545.0000042
CGTTCAT253.8813538E-545.00000417
AGTATGG502.1827873E-1145.0000042
ATCCAAT253.8813538E-545.00000443
CGAATAA253.8813538E-545.00000413
AGCGAAG302.1581473E-645.0000041
CACAACG302.1581473E-645.00000412
TTCCTCG253.8813538E-545.00000412
CGGCGAT207.021882E-445.031
CTCGTAG207.021882E-445.01
TGCTTGG207.021882E-445.02
CGTGACG207.021882E-445.025
TATGCGC207.021882E-445.016
GCGCACT207.021882E-445.019
GTAATGT207.021882E-445.017
GAGACGA207.021882E-445.09
ACCTAAG207.021882E-445.014
TGATTCA800.045.044
ACGTGAC207.021882E-445.024