##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550389_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	505458
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20183872844034	33.0	31.0	34.0	30.0	34.0
2	32.28047434208183	34.0	31.0	34.0	30.0	34.0
3	32.11560802282287	33.0	31.0	34.0	30.0	34.0
4	35.89431960716815	37.0	35.0	37.0	35.0	37.0
5	33.91440238358083	37.0	35.0	37.0	32.0	37.0
6	34.86069070031536	37.0	35.0	37.0	32.0	37.0
7	35.88815885790709	37.0	35.0	37.0	33.0	37.0
8	35.921409889644636	37.0	35.0	37.0	35.0	37.0
9	37.887480265422646	39.0	38.0	39.0	35.0	39.0
10	37.475139378543815	39.0	37.0	39.0	34.0	39.0
11	37.432012155312606	39.0	37.0	39.0	35.0	39.0
12	37.440461917706315	39.0	37.0	39.0	35.0	39.0
13	37.45491613546526	39.0	37.0	39.0	35.0	39.0
14	38.7190627114419	40.0	38.0	41.0	35.0	41.0
15	38.83810722156935	40.0	38.0	41.0	35.0	41.0
16	38.78426694206047	40.0	38.0	41.0	35.0	41.0
17	38.767215080184705	40.0	38.0	41.0	35.0	41.0
18	38.483990756897704	40.0	38.0	41.0	35.0	41.0
19	38.149434770050135	40.0	37.0	41.0	34.0	41.0
20	37.92639546708134	39.0	35.0	41.0	34.0	41.0
21	37.792406095066255	39.0	35.0	41.0	34.0	41.0
22	37.80867450905911	39.0	35.0	41.0	34.0	41.0
23	37.81650898788821	39.0	35.0	41.0	34.0	41.0
24	37.72301358372011	39.0	35.0	41.0	34.0	41.0
25	37.52745430876551	39.0	35.0	41.0	33.0	41.0
26	37.57724281740521	39.0	35.0	41.0	34.0	41.0
27	37.56472545691234	39.0	35.0	41.0	34.0	41.0
28	37.446349647250614	39.0	35.0	41.0	33.0	41.0
29	37.37813824294007	39.0	35.0	41.0	33.0	41.0
30	37.099509751552056	39.0	35.0	41.0	33.0	41.0
31	36.84526508631775	39.0	35.0	41.0	32.0	41.0
32	36.41505525681659	39.0	35.0	41.0	31.0	41.0
33	35.91072848782688	39.0	35.0	41.0	27.0	41.0
34	35.45588159649269	39.0	35.0	41.0	22.0	41.0
35	35.131162233063876	39.0	35.0	41.0	20.0	41.0
36	34.88438406356216	39.0	35.0	41.0	18.0	41.0
37	34.77875313082393	39.0	35.0	41.0	18.0	41.0
38	34.6234662424969	39.0	35.0	41.0	17.0	41.0
39	34.53868175001681	39.0	35.0	41.0	16.0	41.0
40	34.454840956122965	39.0	35.0	41.0	15.0	41.0
41	34.30934123112108	39.0	34.0	41.0	15.0	41.0
42	34.24141076014229	39.0	34.0	41.0	15.0	41.0
43	34.155472858279026	39.0	34.0	41.0	15.0	41.0
44	33.99554859157438	38.0	34.0	41.0	14.0	41.0
45	33.96372794574425	38.0	34.0	41.0	15.0	41.0
46	33.85012206751105	38.0	34.0	41.0	13.0	41.0
47	33.816696936243964	38.0	34.0	41.0	13.0	41.0
48	33.744783938527036	38.0	33.0	40.0	13.0	41.0
49	33.630082024619256	38.0	33.0	40.0	12.0	41.0
50	33.563716471002536	38.0	33.0	40.0	12.0	41.0
51	32.61438141250114	36.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	10.0
10	11.0
11	17.0
12	11.0
13	20.0
14	18.0
15	50.0
16	47.0
17	91.0
18	209.0
19	394.0
20	645.0
21	1049.0
22	1667.0
23	2705.0
24	4615.0
25	8484.0
26	12754.0
27	14763.0
28	13504.0
29	11615.0
30	10541.0
31	10619.0
32	11811.0
33	15292.0
34	25736.0
35	34319.0
36	33157.0
37	47561.0
38	85411.0
39	158223.0
40	108.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.88875435743425	18.547930787523395	21.535122601680058	25.028192253362295
2	42.207265489912125	22.161880908008182	22.014291988651877	13.616561613427821
3	28.08759580420134	20.33957321874419	37.950531992766955	13.622298984287518
4	25.626659386140886	22.09698926518128	37.33287434366456	14.943477005013275
5	20.62723312322686	30.99505794744568	33.82654938689268	14.551159542434783
6	23.070166067210334	30.431608560948685	34.653522152186724	11.844703219654255
7	69.34760158114027	5.606598372169399	21.721686074807405	3.3241139718829262
8	70.9514934969869	5.213489548093016	21.13607856636951	2.698938388550582
9	65.31146010153168	7.624174511037514	22.657471046061197	4.406894341369609
10	33.70111858947726	25.481246710903775	29.63233344808075	11.185301251538208
11	25.64268445647314	23.45417423406099	35.69159059704268	15.211550712423188
12	23.767949067973994	21.058327299201913	39.26280719664146	15.910916436182632
13	20.400310213707172	22.249919874648338	40.328771134298	17.020998777346485
14	17.83709032204456	24.298359111934126	40.40276343435063	17.461787131670683
15	18.485808909939106	24.44990483877988	40.921896576965835	16.142389674315176
16	20.017686929477822	23.613040054762216	40.29236850539511	16.076904510364855
17	19.903335193032852	23.71571920911332	39.106117620059436	17.274827977794395
18	19.41288890471612	24.64774521325214	39.48973010616115	16.449635775870597
19	21.249836781691062	24.748841644607463	37.77089293274614	16.23042864095533
20	21.177822885383158	24.410930285008845	39.15676475592433	15.25448207368367
21	20.811026831111587	24.404797233400203	38.931622409774896	15.852553525713315
22	19.995330967162456	22.865005598882597	39.16368916903086	17.975974264924087
23	19.824594723992895	23.729172354577432	39.66640156056487	16.7798313608648
24	18.689584495645533	24.30726192878538	39.80132869595496	17.201824879614133
25	18.5022296610203	25.347902298509474	38.878799029790805	17.271069010679422
26	17.71462713024623	26.199011589489135	38.527434524728065	17.558926755536564
27	18.85992505806615	24.656648030103394	39.325918276098115	17.157508635732345
28	17.229324691665777	25.912538727253303	39.51485583371912	17.343280747361796
29	18.2333645921125	24.785244273510358	39.503578932374204	17.477812202002934
30	18.50381238401608	24.76407535344183	38.510222412148984	18.221889850393108
31	20.208998571592495	25.562163424062927	36.84994598957777	17.378892014766805
32	19.193879610175326	25.70658689742768	37.760011712150174	17.339521780246827
33	19.352151909753136	26.649296281788004	35.79822655888323	18.200325249575634
34	19.189131441187993	27.09423928397612	35.51986515200076	18.196764122835134
35	20.181300919166382	25.665238259162976	35.19481341674284	18.95864740492781
36	20.038064488048462	28.94721223128331	32.710333994120184	18.30438928654804
37	20.04202129553791	29.073434390196613	33.31493417850741	17.569610135758065
38	19.600639420090292	28.351910544496278	33.34935840366558	18.69809163174784
39	21.34341527881644	26.764835060479804	33.052597841957194	18.839151818746565
40	20.667196878870254	27.277043789988486	34.09086412718762	17.964895203953642
41	18.811454166320445	28.395435426880177	33.459555492246636	19.333554914552742
42	19.185768154821965	28.39583110762912	33.06545746629789	19.352943271251025
43	20.283188712019594	27.072279002409694	33.01223840556485	19.632293880005854
44	19.793929465949695	27.807651674323093	32.85020713887207	19.548211720855146
45	20.190995097515522	27.93822632147478	31.99692160377321	19.873856977236485
46	19.697779043956174	28.432035896157544	32.409418784547874	19.460766275338408
47	20.0970209196412	27.254885668047592	34.12034234298399	18.527751069327223
48	19.31931040759074	26.921722477436305	34.222625816586145	19.53634129838681
49	19.2589690933767	27.089491114988785	34.67488891262973	18.976650879004783
50	18.749134448361684	26.540484075828257	34.68715501584702	20.02322645996304
51	19.27914881157287	25.72617309450043	33.49714516339636	21.49753293053033
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	480.0
1	1261.5
2	2043.0
3	14220.0
4	26397.0
5	17643.5
6	8890.0
7	8322.0
8	7754.0
9	7449.5
10	7145.0
11	6999.0
12	6853.0
13	6587.5
14	6322.0
15	5925.0
16	5528.0
17	5203.5
18	4879.0
19	4786.5
20	4694.0
21	4459.0
22	4224.0
23	4204.0
24	4184.0
25	4862.5
26	6169.0
27	6797.0
28	8203.5
29	9610.0
30	11219.5
31	12829.0
32	13576.5
33	14324.0
34	15807.5
35	17291.0
36	17667.5
37	18044.0
38	19386.5
39	20729.0
40	22364.0
41	23999.0
42	25317.5
43	26636.0
44	28092.5
45	29549.0
46	31234.5
47	32920.0
48	34312.0
49	35704.0
50	33966.5
51	32229.0
52	29483.0
53	26737.0
54	24177.5
55	21618.0
56	19727.0
57	17836.0
58	16737.0
59	15638.0
60	15092.0
61	14546.0
62	13189.0
63	11832.0
64	9679.0
65	7526.0
66	6459.0
67	5392.0
68	4399.5
69	3407.0
70	2678.5
71	1950.0
72	1731.0
73	1512.0
74	1225.0
75	689.5
76	441.0
77	358.0
78	275.0
79	184.5
80	94.0
81	74.5
82	55.0
83	43.0
84	31.0
85	21.5
86	12.0
87	13.0
88	14.0
89	10.0
90	6.0
91	3.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	505458.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.19175338287018
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.28128680868652	27.60753519907971
2	7.731296267427458	5.596183356812786
3	3.1807893119610853	3.453550270240948
4	1.869466378357669	2.706370644923529
5	1.410487755273201	2.5524012494202912
6	1.1009878685335635	2.3908008849299174
7	0.9242664646897272	2.341557676507553
8	0.7711237493489693	2.232665645128968
9	0.66116208064886	2.153575347205398
>10	5.932339235940841	40.320118800844085
>50	0.10108478136671452	2.3672463478747323
>100	0.03131430727121019	2.234031577752419
>500	0.0032962428706537045	0.9201760539193837
>1k	5.493738117756173E-4	0.21095652402948706
>5k	0.0	0.0
>10k+	5.493738117756173E-4	2.9128304213308063
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14650	2.898361486018621	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1061	0.2099086373150687	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	917	0.18141962339106316	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	897	0.17746281590161794	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	867	0.17152760466745012	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	765	0.1513478864712795	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	620	0.12266103217280169	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	562	0.11118629045341058	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05737370859695563	0.0
2	0.0	0.0	0.0	0.2924080734700015	0.0
3	0.0	0.0	0.0	0.41665182863858125	0.0
4	0.0	0.0	0.0	0.5563271330159973	0.0
5	0.0	0.0	0.0	0.9818817785058304	0.0
6	0.0	0.0	0.0	1.2390742653197695	0.0
7	0.0	0.0	0.0	1.458874921358451	0.0
8	0.0	0.0	0.0	1.7746281590161794	0.0
9	0.0	0.0	0.0	1.899465435308176	0.0
10	0.0	0.0	0.0	2.275559987179944	0.0
11	0.0	0.0	0.0	2.9260591384447374	0.0
12	0.0	0.0	0.0	3.409976694403887	0.0
13	0.0	0.0	0.0	3.600496975020674	0.0
14	0.0	0.0	0.0	3.6824028900521903	0.0
15	0.0	0.0	0.0	3.818517067689106	0.0
16	0.0	0.0	0.0	4.100835282061022	0.0
17	0.0	0.0	0.0	4.434196313046781	0.0
18	0.0	0.0	0.0	4.796046357956547	0.0
19	0.0	0.0	0.0	5.048490675783151	0.0
20	1.978403744722608E-4	0.0	0.0	5.2987587494905615	0.0
21	1.978403744722608E-4	0.0	0.0	5.601256682058648	0.0
22	1.978403744722608E-4	0.0	0.0	5.949060060380882	0.0
23	1.978403744722608E-4	0.0	0.0	6.284399495111364	0.0
24	1.978403744722608E-4	0.0	0.0	6.55009911802761	0.0
25	3.956807489445216E-4	0.0	0.0	6.788298928892212	0.0
26	3.956807489445216E-4	0.0	0.0	7.0094844675522	0.0
27	3.956807489445216E-4	0.0	0.0	7.229087283216409	0.0
28	5.935211234167824E-4	0.0	0.0	7.477970474302514	0.0
29	5.935211234167824E-4	0.0	0.0	7.7262601442652015	0.0
30	5.935211234167824E-4	0.0	0.0	8.002247466654005	0.0
31	7.913614978890432E-4	0.0	0.0	8.274673662302307	0.0
32	9.89201872361304E-4	0.0	0.0	8.530481266494942	0.0
33	9.89201872361304E-4	0.0	0.0	8.799148495028271	0.0
34	9.89201872361304E-4	0.0	0.0	9.04743816499096	0.0
35	9.89201872361304E-4	0.0	0.0	9.302652248060175	0.0
36	9.89201872361304E-4	0.0	0.0	9.57527628408295	0.0
37	9.89201872361304E-4	0.0	0.0	9.817432902436998	0.0
38	9.89201872361304E-4	0.0	0.0	10.087682853966106	0.0
39	9.89201872361304E-4	0.0	0.0	10.345073181154516	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGCG	40	6.8012014E-9	45.0	1
ATATGCG	20	7.0295326E-4	45.0	1
CCTCGTA	20	7.0295326E-4	45.0	42
GCGATAT	40	6.8012014E-9	45.0	9
TGTAGCG	25	3.8876948E-5	45.0	1
ATACGGA	20	7.0295326E-4	45.0	12
TGTCGTG	20	7.0295326E-4	45.0	1
CGTTTTT	7605	0.0	43.402367	1
CGTTATT	555	0.0	42.567566	1
GGCGATA	130	0.0	39.807693	8
CTATAGC	40	3.4530967E-7	39.375	24
GCGATAA	40	3.4530967E-7	39.375	9
TCAACGG	40	3.4530967E-7	39.375	2
GCGAAAG	35	6.2413455E-6	38.571426	1
TAGCGGA	35	6.2413455E-6	38.571426	27
TAGGGTA	135	0.0	38.333332	5
ATGGGCG	65	9.094947E-12	38.076927	5
TACGGGT	65	9.094947E-12	38.076927	4
GCGATTC	30	1.13901704E-4	37.500004	9
AAGTGCG	30	1.13901704E-4	37.500004	1
>>END_MODULE