##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550388_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	374566
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.195738534730864	33.0	31.0	34.0	30.0	34.0
2	32.286224590592845	34.0	31.0	34.0	30.0	34.0
3	32.09217334194775	33.0	31.0	34.0	30.0	34.0
4	35.87245505464991	37.0	35.0	37.0	35.0	37.0
5	33.78092245425373	37.0	35.0	37.0	32.0	37.0
6	34.78294879941052	37.0	35.0	37.0	32.0	37.0
7	35.84291152961027	37.0	35.0	37.0	33.0	37.0
8	35.86613841085416	37.0	35.0	37.0	35.0	37.0
9	37.83698200050191	39.0	38.0	39.0	35.0	39.0
10	37.52524521713129	39.0	37.0	39.0	35.0	39.0
11	37.49611550434369	39.0	37.0	39.0	35.0	39.0
12	37.46903616452107	39.0	37.0	39.0	35.0	39.0
13	37.451968411441506	39.0	37.0	39.0	35.0	39.0
14	38.725225994884745	40.0	38.0	41.0	35.0	41.0
15	38.85537662254449	40.0	38.0	41.0	35.0	41.0
16	38.79189782308058	40.0	38.0	41.0	35.0	41.0
17	38.760416054847475	40.0	38.0	41.0	35.0	41.0
18	38.41759529695701	39.0	38.0	41.0	35.0	41.0
19	38.03878355216437	39.0	37.0	41.0	34.0	41.0
20	37.74886401862422	39.0	35.0	41.0	34.0	41.0
21	37.58326703438112	39.0	35.0	41.0	34.0	41.0
22	37.570372110656066	39.0	35.0	41.0	34.0	41.0
23	37.59406886903777	39.0	35.0	41.0	34.0	41.0
24	37.49537598180294	39.0	35.0	41.0	34.0	41.0
25	37.304808765344426	39.0	35.0	41.0	33.0	41.0
26	37.33251816769274	39.0	35.0	41.0	33.0	41.0
27	37.32716530598079	39.0	35.0	41.0	33.0	41.0
28	37.18473646833936	39.0	35.0	41.0	33.0	41.0
29	37.087260990052485	39.0	35.0	41.0	33.0	41.0
30	36.789671246188924	39.0	35.0	41.0	33.0	41.0
31	36.48261454590112	39.0	35.0	41.0	31.0	41.0
32	36.019547956835375	39.0	35.0	41.0	30.0	41.0
33	35.42083905106176	39.0	35.0	41.0	23.0	41.0
34	34.91751520426307	39.0	35.0	41.0	20.0	41.0
35	34.572694798780454	39.0	35.0	41.0	18.0	41.0
36	34.322327707266545	39.0	35.0	41.0	15.0	41.0
37	34.18182109427978	39.0	34.0	41.0	15.0	41.0
38	34.02057314331787	38.0	34.0	41.0	14.0	41.0
39	33.90311720764832	38.0	34.0	41.0	12.0	41.0
40	33.79226090995979	38.0	34.0	41.0	12.0	41.0
41	33.67522679581169	38.0	33.0	41.0	10.0	41.0
42	33.59272598153596	38.0	33.0	41.0	10.0	41.0
43	33.483893359247766	38.0	33.0	41.0	10.0	41.0
44	33.317783781763424	38.0	33.0	41.0	10.0	41.0
45	33.28868076654047	38.0	33.0	41.0	10.0	41.0
46	33.16779953332657	38.0	33.0	40.0	10.0	41.0
47	33.15478447056059	38.0	33.0	40.0	10.0	41.0
48	33.08754131448129	37.0	33.0	40.0	10.0	41.0
49	32.969874468051025	37.0	33.0	40.0	10.0	41.0
50	32.89826092063882	37.0	32.0	40.0	10.0	41.0
51	32.06830304939584	36.0	30.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	8.0
10	12.0
11	12.0
12	7.0
13	18.0
14	9.0
15	29.0
16	46.0
17	89.0
18	195.0
19	344.0
20	579.0
21	880.0
22	1291.0
23	2156.0
24	3652.0
25	6568.0
26	10541.0
27	12804.0
28	11885.0
29	10186.0
30	9001.0
31	8798.0
32	9316.0
33	11729.0
34	19674.0
35	27781.0
36	24902.0
37	32252.0
38	56749.0
39	112957.0
40	92.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.84112279277885	17.284537304507083	19.50844443969821	28.36589546301586
2	44.461590213740706	20.88683970248234	20.37184368041947	14.279726403357484
3	27.048370647629522	19.447573992300423	40.15954464633736	13.344510713732694
4	24.1855907904081	21.07585846019126	39.704084193439876	15.034466555960766
5	19.236930207226496	30.526529369990868	35.60814382512027	14.628396597662361
6	22.19368549200942	29.00743794151098	37.251111953567595	11.54776461291201
7	64.58247678646754	6.172209971006445	25.746864371032075	3.498448871493942
8	65.58443638771271	6.123887378993288	25.308757335155885	2.9829188981381116
9	59.58175595222204	8.55283181068223	27.207221157286032	4.6581910798097
10	32.0450868471778	24.996662804418982	33.221114569928936	9.737135778474288
11	25.025229198592502	22.027092688604945	39.14957577569774	13.798102337104808
12	24.178916399246063	19.757799693511956	41.17431907861365	14.888964828628332
13	19.432890331743938	22.09410357587181	41.16097029628957	17.31203579609468
14	15.22962575353876	24.001110618689363	42.96919634990896	17.80006727786291
15	16.4344868461099	23.638290715120966	45.01583165583635	14.911390782932783
16	18.205603284868353	21.828195831976206	44.54675544496831	15.419445438187129
17	17.743735416455312	23.10247059263254	41.58305879337687	17.57073519753528
18	17.57580773481843	24.195201913681434	41.819866191805986	16.40912415969415
19	20.32939455262891	23.12863420598773	40.549062114553905	15.99290912682945
20	21.380210697180203	22.304747360945733	42.471820720513875	13.843221221360189
21	19.911310690238835	23.423108344056857	41.22637932967755	15.43920163602676
22	18.427193071448023	22.675042582615614	41.73977349786153	17.15799084807484
23	19.386970520549117	22.70868151407229	41.937068500611375	15.967279464767223
24	17.793659862347358	22.4352984520752	43.287965271807906	16.483076413769535
25	17.798732399630506	23.959195442191763	41.1807264941292	17.061345664048524
26	16.957759113213694	25.379238905827012	40.47484288483204	17.18815909612725
27	17.891105973313117	24.677092955580594	41.78035379612672	15.651447274979576
28	16.66915843936716	24.359658911914057	42.96092010486803	16.01026254385075
29	17.421495811152106	24.08066936134086	41.132403902116046	17.36543092539099
30	17.085907423524826	24.5454739618652	40.899334162737674	17.469284451872298
31	18.53505123262656	25.37496729548331	39.169331973537375	16.92064949835276
32	18.822850979533648	25.41821735021331	38.73336074283304	17.02557092742
33	18.175435036815944	26.700768355910576	38.13400041648201	16.989796190791477
34	17.300288867649492	27.596471649856102	36.937949520244764	18.16528996224964
35	18.8321951271605	25.67104328743132	35.98831714570997	19.50844443969821
36	18.834864883625315	28.799463912901864	34.57895270793398	17.786718495538835
37	19.36854920094189	29.215678945766566	35.02480203755813	16.39096981573341
38	17.93622485756849	28.694809459481107	34.42811146767192	18.94085421527848
39	19.826946385950674	27.15062232023195	34.942306562795345	18.08012473102204
40	19.004928370434047	27.709402348317784	35.32007710256671	17.96559217868146
41	16.881404078319974	28.135762455748786	35.77046501818104	19.212368447750197
42	17.94076344355868	27.77214162524095	34.007891800109995	20.27920313109038
43	19.547956835377477	27.890678812278747	34.18810036148503	18.373263990858753
44	18.548133039304155	28.165396752508236	33.64667375042048	19.639796457767122
45	18.493670007421922	28.155785629234902	33.23099266884875	20.11955169449443
46	17.787519422478283	29.466636053459204	33.445107137326936	19.30073738673558
47	18.96114436441108	27.18052359263788	35.403640479915424	18.45469156303562
48	17.81154723066162	26.784331733259293	35.76405760266548	19.640063433413605
49	17.510398701430454	28.018293171296914	35.30699529588911	19.16431283138352
50	16.162972613638185	27.411991478137367	36.157045754286294	20.267990153938158
51	17.065083323099266	26.461291201016646	35.407111163319684	21.066514312564408
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	388.0
1	978.5
2	1569.0
3	14606.5
4	27644.0
5	18265.5
6	8887.0
7	8238.5
8	7590.0
9	7075.5
10	6561.0
11	6137.0
12	5713.0
13	5454.5
14	5196.0
15	4796.5
16	4397.0
17	4117.5
18	3838.0
19	3575.0
20	3312.0
21	3332.5
22	3353.0
23	3185.5
24	3018.0
25	3505.0
26	4371.5
27	4751.0
28	5953.0
29	7155.0
30	8243.5
31	9332.0
32	10374.0
33	11416.0
34	12058.5
35	12701.0
36	13467.0
37	14233.0
38	15071.0
39	15909.0
40	16673.5
41	17438.0
42	18359.0
43	19280.0
44	20038.0
45	20796.0
46	20988.0
47	21180.0
48	21663.0
49	22146.0
50	21034.0
51	19922.0
52	18046.0
53	16170.0
54	14688.0
55	13206.0
56	12382.0
57	11558.0
58	11760.5
59	11963.0
60	11995.0
61	12027.0
62	10757.0
63	9487.0
64	8083.5
65	6680.0
66	5613.0
67	4546.0
68	3577.5
69	2609.0
70	2273.5
71	1938.0
72	1569.0
73	1200.0
74	937.0
75	503.5
76	333.0
77	270.0
78	207.0
79	170.5
80	134.0
81	94.5
82	55.0
83	45.0
84	35.0
85	20.5
86	6.0
87	6.0
88	6.0
89	4.5
90	3.0
91	4.5
92	6.0
93	5.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	374566.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.38949765083729
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.90429969964487	25.232811321117715
2	7.106778184624578	4.60369950631839
3	2.712148997062781	2.63535430707257
4	1.5323832022581312	1.9853248851888903
5	1.0949286696800025	1.773209478721742
6	0.854422842765926	1.6604595995153206
7	0.7450763847703318	1.68928548699491
8	0.6763520411275148	1.7525362277790917
9	0.5530005712329521	1.6120269632575246
>10	6.482848928982657	43.396515045958076
>50	0.2914011965767019	5.812890271760985
>100	0.04139221542282698	2.5440612066270685
>500	0.0033113772338261576	0.8033313000690027
>1k	8.278443084565394E-4	0.2788599973537259
>5k	0.0	0.0
>10k+	8.278443084565394E-4	4.219634402264985
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15737	4.201395748679805	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1040	0.2776546723407891	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	927	0.24748642428837642	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	763	0.20370241826540583	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	654	0.1746020727989193	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	652	0.1740681215059562	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	477	0.12734738337168883	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6697564648152795E-4	0.0	0.0	0.04912351895260114	0.0
2	2.6697564648152795E-4	0.0	0.0	0.2616361335518974	0.0
3	2.6697564648152795E-4	0.0	0.0	0.3494711212443201	0.0
4	2.6697564648152795E-4	0.0	0.0	0.44851908608896696	0.0
5	2.6697564648152795E-4	0.0	0.0	0.7312462957129051	0.0
6	2.6697564648152795E-4	0.0	0.0	0.8882279758440436	0.0
7	2.6697564648152795E-4	0.0	0.0	1.0433408264498112	0.0
8	2.6697564648152795E-4	0.0	0.0	1.2665324669083686	0.0
9	2.6697564648152795E-4	0.0	0.0	1.3621097483487556	0.0
10	2.6697564648152795E-4	0.0	0.0	1.5871702183326837	0.0
11	2.6697564648152795E-4	0.0	0.0	1.9753528083168252	0.0
12	2.6697564648152795E-4	0.0	0.0	2.2449982112631686	0.0
13	5.339512929630559E-4	0.0	0.0	2.371277692048931	0.0
14	5.339512929630559E-4	0.0	0.0	2.428410480395978	0.0
15	5.339512929630559E-4	0.0	0.0	2.5301282017054403	0.0
16	5.339512929630559E-4	0.0	0.0	2.7263553018693636	0.0
17	5.339512929630559E-4	0.0	0.0	2.9439404537518086	0.0
18	5.339512929630559E-4	0.0	0.0	3.1714037045540704	0.0
19	5.339512929630559E-4	0.0	0.0	3.3179733344724296	0.0
20	5.339512929630559E-4	0.0	0.0	3.477624771068383	0.0
21	5.339512929630559E-4	0.0	0.0	3.6837299701521227	0.0
22	5.339512929630559E-4	0.0	0.0	3.9216052711671643	0.0
23	5.339512929630559E-4	0.0	0.0	4.130914178008682	0.0
24	5.339512929630559E-4	0.0	0.0	4.321534789596493	0.0
25	5.339512929630559E-4	0.0	0.0	4.481987153131891	0.0
26	5.339512929630559E-4	0.0	0.0	4.649113907829328	0.0
27	5.339512929630559E-4	0.0	0.0	4.808765344425281	0.0
28	5.339512929630559E-4	0.0	0.0	4.9761590747692	0.0
29	5.339512929630559E-4	0.0	0.0	5.147023488517378	0.0
30	5.339512929630559E-4	0.0	0.0	5.348590101610931	0.0
31	5.339512929630559E-4	0.0	0.0	5.535206078501519	0.0
32	5.339512929630559E-4	0.0	0.0	5.717550445048403	0.0
33	5.339512929630559E-4	0.0	0.0	5.903365494999546	0.0
34	5.339512929630559E-4	0.0	0.0	6.109203718436804	0.0
35	5.339512929630559E-4	0.0	0.0	6.296620622266837	0.0
36	5.339512929630559E-4	0.0	0.0	6.503259772643539	0.0
37	8.009269394445838E-4	0.0	0.0	6.716039362889317	0.0
38	8.009269394445838E-4	0.0	0.0	6.914402268225093	0.0
39	8.009269394445838E-4	0.0	0.0	7.106624733691793	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTT	25	3.8857543E-5	45.0	16
GCGCGAC	20	7.02719E-4	45.0	9
CGGTTAT	20	7.02719E-4	45.0	5
ATTACGA	20	7.02719E-4	45.0	32
GGCCGAT	20	7.02719E-4	45.0	8
TTACTCA	25	3.8857543E-5	45.0	34
CGCGACC	20	7.02719E-4	45.0	10
AAAACCG	25	3.8857543E-5	45.0	14
ATACTCC	25	3.8857543E-5	45.0	10
ATCCGAT	20	7.02719E-4	45.0	26
TCAATAG	25	3.8857543E-5	45.0	23
TGCGACG	20	7.02719E-4	45.0	1
TATTACG	20	7.02719E-4	45.0	42
GTATACG	20	7.02719E-4	45.0	1
ACCGTTT	25	3.8857543E-5	45.0	17
GCGTGAC	20	7.02719E-4	45.0	14
TAGGACG	20	7.02719E-4	45.0	1
ATTGCGT	20	7.02719E-4	45.0	12
CAATCGG	35	1.2089913E-7	45.0	2
AGGGCCG	25	3.8857543E-5	45.0	6
>>END_MODULE