FastQCFastQC Report
Sat 18 Jun 2016
SRR3550387_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550387_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518301
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169603.2722298432763974No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9840.18985107109575325No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7890.15222814542128993No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC6680.1288826376950845No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5350.10322187300429674No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5280.10187130644162369No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC253.887831E-545.00000425
TAATACG253.887831E-545.0000041
CGTGTAC1350.045.00000418
GACCGGT207.029699E-445.09
TGCGACG207.029699E-445.01
ACTCGGT351.21021E-745.045
ACTCGAA207.029699E-445.044
AACTCGA207.029699E-445.043
TCGAACA207.029699E-445.038
TGCGTAG351.21021E-745.01
CGTTATT4900.044.5408171
GCCCTCG1500.043.50000413
CCCTCGT1400.043.39285714
CGTTTTT79750.043.1097151
TCGTGTA1450.041.89655317
GTTACGG700.041.7857132
GCTACGA603.6379788E-1241.25000410
CCTCGTG1500.040.50000415
TGTTGCG451.9248546E-840.01
CGTAAGG451.9248546E-840.02