##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550387_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	518301
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26088315476914	33.0	31.0	34.0	30.0	34.0
2	32.35592638254605	34.0	31.0	34.0	31.0	34.0
3	32.17277798036276	34.0	31.0	34.0	30.0	34.0
4	35.93378750957455	37.0	35.0	37.0	35.0	37.0
5	33.99957553622316	37.0	35.0	37.0	33.0	37.0
6	34.91660637351655	37.0	35.0	37.0	32.0	37.0
7	35.92654268465621	37.0	35.0	37.0	33.0	37.0
8	35.965766996397846	37.0	35.0	37.0	35.0	37.0
9	37.93000399381826	39.0	38.0	39.0	35.0	39.0
10	37.57102147207897	39.0	37.0	39.0	35.0	39.0
11	37.484567847640655	39.0	37.0	39.0	35.0	39.0
12	37.52373620733898	39.0	37.0	39.0	35.0	39.0
13	37.53676724528797	39.0	37.0	39.0	35.0	39.0
14	38.831989519603475	40.0	38.0	41.0	35.0	41.0
15	38.952801557396185	40.0	38.0	41.0	35.0	41.0
16	38.90350201909701	40.0	38.0	41.0	35.0	41.0
17	38.8645902670456	40.0	38.0	41.0	35.0	41.0
18	38.574897598113836	40.0	38.0	41.0	35.0	41.0
19	38.2430788287115	40.0	37.0	41.0	35.0	41.0
20	37.978485474656615	40.0	35.0	41.0	34.0	41.0
21	37.85481602389345	40.0	35.0	41.0	34.0	41.0
22	37.864690594847396	40.0	35.0	41.0	34.0	41.0
23	37.8646423603273	40.0	35.0	41.0	34.0	41.0
24	37.780253173349074	40.0	35.0	41.0	34.0	41.0
25	37.58790162473157	39.0	35.0	41.0	34.0	41.0
26	37.62220601542347	39.0	35.0	41.0	34.0	41.0
27	37.59737102571672	40.0	35.0	41.0	34.0	41.0
28	37.47873918823232	39.0	35.0	41.0	33.0	41.0
29	37.415715964275584	40.0	35.0	41.0	33.0	41.0
30	37.13448170078777	39.0	35.0	41.0	33.0	41.0
31	36.87825221251744	39.0	35.0	41.0	33.0	41.0
32	36.467064504988414	39.0	35.0	41.0	31.0	41.0
33	35.974618995525766	40.0	35.0	41.0	27.0	41.0
34	35.51663801536173	40.0	35.0	41.0	22.0	41.0
35	35.2172096908939	40.0	35.0	41.0	20.0	41.0
36	34.99041676554743	39.0	35.0	41.0	18.0	41.0
37	34.86252582958551	39.0	35.0	41.0	18.0	41.0
38	34.73539314027949	39.0	35.0	41.0	18.0	41.0
39	34.59217134445043	39.0	35.0	41.0	15.0	41.0
40	34.4982317224933	39.0	35.0	41.0	15.0	41.0
41	34.343275432615414	39.0	35.0	41.0	15.0	41.0
42	34.25566610907561	39.0	34.0	41.0	15.0	41.0
43	34.16437552696213	39.0	34.0	41.0	14.0	41.0
44	34.02076978435311	39.0	34.0	41.0	12.0	41.0
45	33.99639205789686	39.0	34.0	41.0	13.0	41.0
46	33.90161508467088	38.0	34.0	41.0	12.0	41.0
47	33.87099967007588	38.0	34.0	41.0	12.0	41.0
48	33.787258755047745	38.0	34.0	41.0	12.0	41.0
49	33.691252766249725	38.0	33.0	41.0	12.0	41.0
50	33.63448845362058	38.0	33.0	41.0	12.0	41.0
51	32.77689798013124	37.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	9.0
10	13.0
11	7.0
12	10.0
13	9.0
14	17.0
15	40.0
16	54.0
17	113.0
18	193.0
19	407.0
20	672.0
21	1113.0
22	1662.0
23	2686.0
24	4554.0
25	8071.0
26	12704.0
27	15523.0
28	14611.0
29	12663.0
30	11282.0
31	10974.0
32	12123.0
33	14856.0
34	24341.0
35	33814.0
36	31803.0
37	45097.0
38	83697.0
39	175037.0
40	141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.969839919274705	19.138492883478904	21.687011987242936	27.20465521000345
2	42.32675607417312	20.820141192087224	22.458571370690002	14.394531363049657
3	26.879361606479634	20.54250329441772	39.274282704451664	13.303852394650983
4	25.1425330068821	21.891140476286946	38.4186023179581	14.547724198872855
5	20.81435304967577	30.661140920044534	34.701457261321124	13.823048768958579
6	22.660384602769433	30.368453852105244	35.26946696996533	11.701694575159994
7	69.35390825022526	5.096073517126149	22.709004999025662	2.8410132336229332
8	71.28791956797305	4.914711721567198	21.415162231984887	2.3822064784748633
9	65.43282764262466	7.262961097894853	23.096231726352062	4.207979533128433
10	32.3379657766433	29.227418044726903	28.910034902498744	9.524581276131052
11	23.643211184234644	21.946706643436922	39.89303512823629	14.517047044092141
12	22.920272197043804	20.3267985205508	41.123208328751055	15.629720953654344
13	19.89789716786192	20.79795331284331	41.972521758592016	17.33162776070276
14	16.059201120584373	23.831904626848104	41.59802894457082	18.510865307996703
15	16.80374917277798	24.022720388345768	42.64587565912471	16.527654779751536
16	18.954044078633846	22.695692271479313	42.33852529707641	16.011738352810433
17	18.44179347522	23.86875580020104	40.4813033353206	17.208147389258365
18	18.353620772485485	23.848497301760947	40.94126771895096	16.85661420680261
19	19.692418112255233	23.54539157748104	39.73154595495668	17.03064435530705
20	20.286088585590225	23.5745252276187	41.39370751744643	14.745678669344647
21	20.51124732539586	23.578769865387102	40.71688073146685	15.193102077750186
22	19.584372787241392	21.995712915853915	41.44406435642609	16.9758499404786
23	19.340113177477953	22.300555082857258	41.22546551135344	17.133866228311348
24	17.807798943085196	23.24402229592457	42.31498685126982	16.633191909720413
25	18.115728119374648	25.46763367232554	39.864287354259396	16.552350854040412
26	17.83307383161522	25.65709886726053	39.64916139463362	16.86066590649063
27	17.55678650050839	25.206009635327735	40.678678991551244	16.558524872612633
28	16.431571615721367	25.403192353477998	41.621181514216644	16.54405451658399
29	17.485592348847483	23.1872985002923	41.41570245860996	17.911406692250257
30	17.249822014620847	24.45548050264229	40.80871925772861	17.485978225008246
31	18.726184205702864	24.8319026974673	39.40065714710178	17.041255949728054
32	18.44179347522	25.31752784578845	39.20250973854961	17.038168940441945
33	18.14582645991422	26.161439009378718	37.579900482538136	18.11283404816892
34	17.772105398214553	26.511814563352182	37.80467334618301	17.911406692250257
35	19.18151807540406	25.838846538980242	36.57913065959741	18.40050472601828
36	19.19193673174468	27.96830413215487	34.506203923974674	18.33355521212577
37	18.89056745018821	28.664038850011863	35.07209131373468	17.37330238606524
38	19.135019998032032	27.404539061279067	35.04488704440084	18.415553896288063
39	19.359985799757283	26.80353694088956	35.1772425675428	18.659234691810358
40	19.339341425156427	26.827654200937296	35.84770239686977	17.98530197703651
41	18.228403958317656	27.450265386329566	35.39063208444514	18.93069857090764
42	18.538455453491313	28.060914410738164	34.274292351355676	19.126337784414847
43	19.82747476852254	27.703014271629804	33.722875317624315	18.746635642223342
44	18.459350840534746	28.345883955462174	33.84230398938069	19.35246121462239
45	18.740847499811885	27.657480884659684	33.795034159687134	19.806637455841297
46	18.54462947206353	28.4562445374406	33.615022930690856	19.384103059805017
47	19.186920341654755	27.050690621858724	35.521251164863656	18.241137871622858
48	18.733515852757375	26.386983625345117	35.58009727938013	19.29940324251738
49	18.328538822035846	27.511812633971378	35.50986781812113	18.649780725871643
50	17.655763735744287	27.085805352488222	35.92892932871054	19.32950158305695
51	18.055338500215125	25.67253391369108	35.56504810911034	20.707079476983452
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	342.0
1	1153.0
2	1964.0
3	16577.0
4	31190.0
5	20699.0
6	10208.0
7	9539.0
8	8870.0
9	8422.5
10	7975.0
11	7607.5
12	7240.0
13	6803.5
14	6367.0
15	5973.5
16	5580.0
17	5216.5
18	4853.0
19	4658.5
20	4464.0
21	4679.0
22	4894.0
23	4796.5
24	4699.0
25	5283.0
26	6850.5
27	7834.0
28	9464.5
29	11095.0
30	12806.0
31	14517.0
32	15826.0
33	17135.0
34	18696.5
35	20258.0
36	21083.5
37	21909.0
38	21978.5
39	22048.0
40	23893.0
41	25738.0
42	27505.5
43	29273.0
44	29640.0
45	30007.0
46	32085.0
47	34163.0
48	32751.5
49	31340.0
50	28906.0
51	26472.0
52	24513.0
53	22554.0
54	20910.0
55	19266.0
56	17825.0
57	16384.0
58	16097.0
59	15810.0
60	15033.5
61	14257.0
62	12831.0
63	11405.0
64	9910.0
65	8415.0
66	6804.0
67	5193.0
68	4215.0
69	3237.0
70	2708.0
71	2179.0
72	1846.5
73	1514.0
74	1213.0
75	683.5
76	455.0
77	335.5
78	216.0
79	165.0
80	114.0
81	84.5
82	55.0
83	32.0
84	9.0
85	6.5
86	4.0
87	7.0
88	10.0
89	7.5
90	5.0
91	3.0
92	1.0
93	2.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	518301.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.28651803738231
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.83958317631378	23.727564869932973
2	7.23823426133805	4.529182935523031
3	2.9263346943146424	2.746644695912782
4	1.6913780688497377	2.1166932183640075
5	1.2272843023800108	1.9198726231704186
6	0.9900638108734804	1.8585389566231565
7	0.845443297510589	1.8515683884004315
8	0.7401547391474939	1.8525491677433652
9	0.6593367343018327	1.8565515567398734
>10	7.603124273960479	47.241821911461386
>50	0.19582133643447344	3.808135376277245
>100	0.03953490704039842	2.5358579106848222
>500	0.003088664612531127	0.6772079962685479
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	6.177329225062253E-4	3.2778103928979943
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16960	3.2722298432763974	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	984	0.18985107109575325	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	789	0.15222814542128993	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	668	0.1288826376950845	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	535	0.10322187300429674	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	528	0.10187130644162369	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.858761607637261E-4	0.0	0.0	0.04167462536248242	0.0
2	3.858761607637261E-4	0.0	0.0	0.20702256024973906	0.0
3	3.858761607637261E-4	0.0	0.0	0.2924941298589044	0.0
4	5.788142411455892E-4	0.0	0.0	0.38452559420105303	0.0
5	5.788142411455892E-4	0.0	0.0	0.719851977904731	0.0
6	5.788142411455892E-4	0.0	0.0	0.8732377518083122	0.0
7	5.788142411455892E-4	0.0	0.0	1.0302893492391487	0.0
8	5.788142411455892E-4	0.0	0.0	1.232488457479341	0.0
9	5.788142411455892E-4	0.0	0.0	1.305997866104831	0.0
10	5.788142411455892E-4	0.0	0.0	1.5923179773915157	0.0
11	5.788142411455892E-4	0.0	0.0	1.933046627345886	0.0
12	5.788142411455892E-4	0.0	0.0	2.262391930557726	0.0
13	5.788142411455892E-4	0.0	0.0	2.3659996797227865	0.0
14	5.788142411455892E-4	0.0	0.0	2.4153918283005433	0.0
15	5.788142411455892E-4	0.0	0.0	2.509545611526893	0.0
16	5.788142411455892E-4	0.0	0.0	2.6633172615912377	0.0
17	5.788142411455892E-4	0.0	0.0	2.8803726020208336	0.0
18	5.788142411455892E-4	0.0	0.0	3.0958844378073747	0.0
19	5.788142411455892E-4	0.0	0.0	3.2681781435883783	0.0
20	5.788142411455892E-4	0.0	0.0	3.41751221780394	0.0
21	5.788142411455892E-4	0.0	0.0	3.5955940659963996	0.0
22	5.788142411455892E-4	0.0	0.0	3.7962496695935375	0.0
23	5.788142411455892E-4	0.0	0.0	3.9833996075639444	0.0
24	5.788142411455892E-4	0.0	0.0	4.144309966602418	0.0
25	5.788142411455892E-4	0.0	0.0	4.282839508316596	0.0
26	5.788142411455892E-4	0.0	0.0	4.414037402976263	0.0
27	5.788142411455892E-4	0.0	0.0	4.543498854912493	0.0
28	5.788142411455892E-4	0.0	0.0	4.674889687652541	0.0
29	5.788142411455892E-4	0.0	0.0	4.824030823787722	0.0
30	5.788142411455892E-4	0.0	0.0	4.992851644121852	0.0
31	5.788142411455892E-4	0.0	0.0	5.147780922668488	0.0
32	5.788142411455892E-4	0.0	0.0	5.310427724430398	0.0
33	5.788142411455892E-4	0.0	0.0	5.459182984404815	0.0
34	5.788142411455892E-4	0.0	0.0	5.614884015272978	0.0
35	5.788142411455892E-4	0.0	0.0	5.790457668420474	0.0
36	5.788142411455892E-4	0.0	0.0	5.9542620986646755	0.0
37	5.788142411455892E-4	0.0	0.0	6.114979519622768	0.0
38	5.788142411455892E-4	0.0	0.0	6.285729720760716	0.0
39	5.788142411455892E-4	0.0	0.0	6.46323275471203	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	25	3.887831E-5	45.000004	25
TAATACG	25	3.887831E-5	45.000004	1
CGTGTAC	135	0.0	45.000004	18
GACCGGT	20	7.029699E-4	45.0	9
TGCGACG	20	7.029699E-4	45.0	1
ACTCGGT	35	1.21021E-7	45.0	45
ACTCGAA	20	7.029699E-4	45.0	44
AACTCGA	20	7.029699E-4	45.0	43
TCGAACA	20	7.029699E-4	45.0	38
TGCGTAG	35	1.21021E-7	45.0	1
CGTTATT	490	0.0	44.540817	1
GCCCTCG	150	0.0	43.500004	13
CCCTCGT	140	0.0	43.392857	14
CGTTTTT	7975	0.0	43.109715	1
TCGTGTA	145	0.0	41.896553	17
GTTACGG	70	0.0	41.785713	2
GCTACGA	60	3.6379788E-12	41.250004	10
CCTCGTG	150	0.0	40.500004	15
TGTTGCG	45	1.9248546E-8	40.0	1
CGTAAGG	45	1.9248546E-8	40.0	2
>>END_MODULE