FastQCFastQC Report
Sat 18 Jun 2016
SRR3550385_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550385_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530437
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150122.830119316714332No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9020.17004846946951288No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8230.15515508910577505No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7560.1425239943669088No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6310.11895851910782997No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6150.11594213827466786No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA5620.10595037676481844No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG351.2102646E-745.0000041
TAACGCG253.8879545E-545.0000041
GTCTGCG207.0298475E-445.01
TAGCGCG207.0298475E-445.01
TCTAGCG207.0298475E-445.01
ATCGTCC207.0298475E-445.023
TGCGACG207.0298475E-445.01
ATGCCGG207.0298475E-445.02
TTACGAG302.1632823E-644.9999961
CGTTTTT70500.043.2765961
CGTAAGG900.042.52
TACGAAT800.042.187512
CGAATAT800.042.187514
CGTTATT4500.042.01
TAAACGG556.002665E-1140.9090922
TCGACGG556.002665E-1140.9090922
AAGTACG403.4535333E-739.3751
CCTCGTG403.4535333E-739.37515
GTCGATG356.2419276E-638.571431
GGCCGAT1050.038.571438