##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550385_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	530437
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.285115857302564	33.0	31.0	34.0	31.0	34.0
2	32.36131152238626	34.0	31.0	34.0	31.0	34.0
3	32.221913252657714	34.0	31.0	34.0	30.0	34.0
4	35.955474448426486	37.0	35.0	37.0	35.0	37.0
5	33.92890767423841	37.0	35.0	37.0	32.0	37.0
6	34.888887841534434	37.0	35.0	37.0	32.0	37.0
7	35.92505801820009	37.0	35.0	37.0	33.0	37.0
8	35.97124446446986	37.0	35.0	37.0	35.0	37.0
9	37.95133258049495	39.0	38.0	39.0	35.0	39.0
10	37.58767770724893	39.0	37.0	39.0	35.0	39.0
11	37.47387342888977	39.0	37.0	39.0	35.0	39.0
12	37.51965454898508	39.0	37.0	39.0	35.0	39.0
13	37.55653357514653	39.0	37.0	39.0	35.0	39.0
14	38.83386151418547	40.0	38.0	41.0	35.0	41.0
15	38.96415596951193	40.0	38.0	41.0	35.0	41.0
16	38.908656447419766	40.0	38.0	41.0	35.0	41.0
17	38.871830207922905	40.0	38.0	41.0	35.0	41.0
18	38.61095851156688	40.0	38.0	41.0	35.0	41.0
19	38.31571138514093	40.0	37.0	41.0	35.0	41.0
20	38.12486496982677	40.0	35.0	41.0	35.0	41.0
21	37.992506178867615	40.0	35.0	41.0	34.0	41.0
22	38.00001885238021	40.0	35.0	41.0	34.0	41.0
23	38.005444567403856	40.0	35.0	41.0	34.0	41.0
24	37.934602978299026	40.0	35.0	41.0	34.0	41.0
25	37.736045939480086	40.0	35.0	41.0	34.0	41.0
26	37.78192509195249	40.0	35.0	41.0	34.0	41.0
27	37.77616380456114	40.0	35.0	41.0	34.0	41.0
28	37.664395583264366	40.0	35.0	41.0	34.0	41.0
29	37.597741107803564	40.0	35.0	41.0	34.0	41.0
30	37.32481519954302	40.0	35.0	41.0	33.0	41.0
31	37.11493919164764	40.0	35.0	41.0	33.0	41.0
32	36.77542856173306	40.0	35.0	41.0	32.0	41.0
33	36.352215248936254	40.0	35.0	41.0	30.0	41.0
34	35.95199429904022	40.0	35.0	41.0	25.0	41.0
35	35.694506604931405	40.0	35.0	41.0	23.0	41.0
36	35.45640858386576	40.0	35.0	41.0	22.0	41.0
37	35.361013654778986	40.0	35.0	41.0	21.0	41.0
38	35.226268152485595	39.0	35.0	41.0	20.0	41.0
39	35.12732332020579	39.0	35.0	41.0	19.0	41.0
40	35.02564489279594	39.0	35.0	41.0	18.0	41.0
41	34.89140651953012	39.0	35.0	41.0	18.0	41.0
42	34.83725305738476	39.0	35.0	41.0	18.0	41.0
43	34.75276234500987	39.0	35.0	41.0	18.0	41.0
44	34.58553984733342	39.0	35.0	41.0	17.0	41.0
45	34.5350644845665	39.0	35.0	41.0	18.0	41.0
46	34.438659067900616	39.0	34.0	41.0	18.0	41.0
47	34.42578854793312	39.0	34.0	41.0	18.0	41.0
48	34.34596191442151	38.0	34.0	41.0	18.0	41.0
49	34.248917402066596	38.0	34.0	41.0	18.0	41.0
50	34.18435177033276	38.0	34.0	41.0	17.0	41.0
51	33.30153628046309	37.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	5.0
10	13.0
11	17.0
12	14.0
13	13.0
14	20.0
15	35.0
16	63.0
17	120.0
18	202.0
19	378.0
20	613.0
21	938.0
22	1414.0
23	2483.0
24	4016.0
25	7037.0
26	11275.0
27	13999.0
28	13313.0
29	11252.0
30	10543.0
31	10894.0
32	11725.0
33	14903.0
34	25505.0
35	34706.0
36	32888.0
37	47668.0
38	89271.0
39	184961.0
40	144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.12764004019328	19.01507624845175	22.288603547640907	23.56868016371407
2	40.984886046787835	21.718130522569126	22.534815633147762	14.762167797495273
3	28.432217209583794	20.74214279923912	36.952927491860486	13.8727124993166
4	25.54761451407047	22.850215954015273	36.24275832945288	15.359411202461368
5	21.097887213750173	31.181271291406897	32.40705305248314	15.31378844235979
6	24.142546617223157	30.563667315816957	33.46222077268365	11.83156529427623
7	71.42111127240369	5.70586139353024	19.78934350356404	3.08368383050202
8	73.51052057077466	5.067896847316458	19.051461342251766	2.370121239657113
9	67.9735765039015	7.797721501328151	20.44993090602654	3.778771088743809
10	33.79835871177916	30.544626411807624	26.37485695756518	9.282157918848045
11	24.209661090761013	22.875101095888862	38.03109511591386	14.884142697436264
12	24.017555336449004	20.946691124487923	39.53117900900578	15.50457453005729
13	20.76438860788369	21.669114334030244	39.76306328555512	17.80343377253095
14	16.629307533222608	24.843101065725055	39.78172714196031	18.74586425909203
15	17.268591746050898	25.166419386279614	41.04898413949253	16.516004728176956
16	19.821581073718463	23.574901449182466	40.57164187264463	16.03187560445444
17	19.595918082637525	23.6876386828219	39.074008788979654	17.642434445560927
18	19.10028900698858	25.281041857939773	38.59723209353797	17.02143704153368
19	20.636757993880515	24.399693083250227	38.13647992127246	16.827069001596797
20	21.495106864717208	23.788875964534903	39.54192486572392	15.17409230502397
21	21.516787101955558	23.785482536097597	38.5508552382281	16.146875123718747
22	21.072436500470367	22.690347769857684	38.41851152917312	17.818704200498832
23	19.573106702586735	23.944219577442748	39.67030957493539	16.81236414503513
24	18.84106877913871	23.872769056457223	40.360872261927426	16.925289902476635
25	19.00433039173361	25.26727962038847	38.51541276343845	17.21297722443947
26	18.326964370886646	26.356758672566205	37.66271960666394	17.653557349883208
27	18.789036209766664	25.8560394542613	38.64813352009758	16.70679081587446
28	17.521213640828222	26.189161012523638	39.39336810969069	16.896257236957453
29	18.796388638047496	24.332955657316514	39.08890216934339	17.781753535292598
30	18.49776693556445	25.035772391443285	38.547461809790796	17.918998863201473
31	19.12479710125802	26.167857822889427	37.35825366631664	17.34909140953591
32	19.39005009077421	26.440840288290595	36.963107777172404	17.206001843762785
33	19.201526288701583	27.080690072525105	35.62213797302978	18.09564566574353
34	18.9967894396507	27.144411117625655	35.5159990724629	18.342800370260747
35	20.30948067348243	26.161259489816885	34.77415791130709	18.755101925393593
36	19.366861663119277	29.003255806061794	33.04143564645754	18.588446884361385
37	20.103424157817045	28.877133382475208	33.79176037870661	17.227682081001134
38	19.643237556957754	28.051776177001226	33.91901394510564	18.38597232093538
39	20.240480961923847	26.725134181816124	33.586269434447445	19.44811542181258
40	20.07439149229786	27.3259595390216	34.60618320366038	17.99346576502016
41	18.08508833282746	28.16243964881786	34.56847844324585	19.183993575108826
42	18.65461873888888	28.547028205046026	33.18980387868871	19.60854917737639
43	20.01199011381182	27.51222105546936	33.21299230634364	19.262796524375183
44	19.72317164903655	28.59170834613724	32.439102098835484	19.24601790599072
45	19.865318595799312	28.193734599961918	32.23455377358668	19.706393030652087
46	18.79940501888066	29.018903281633822	32.752051610276055	19.429640089209464
47	19.53050032331832	27.505811246198892	34.68159272448943	18.28209570599336
48	18.910822585905585	26.89329741326491	34.69026481938477	19.505615181444732
49	18.799970590286875	28.21466074199198	33.48314691471372	19.502221753007426
50	18.06491628600569	27.166845450072298	34.5846914902241	20.183546773697913
51	18.21196485162234	26.616732995624364	33.95483346749944	21.216468685253854
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	398.0
1	1181.0
2	1964.0
3	14742.0
4	27520.0
5	18214.0
6	8908.0
7	8363.0
8	7818.0
9	7462.5
10	7107.0
11	6751.0
12	6395.0
13	6105.0
14	5815.0
15	5449.5
16	5084.0
17	4713.5
18	4343.0
19	4211.5
20	4080.0
21	4177.0
22	4274.0
23	4287.5
24	4301.0
25	5054.0
26	6813.0
27	7819.0
28	9220.5
29	10622.0
30	13350.0
31	16078.0
32	17142.0
33	18206.0
34	19393.0
35	20580.0
36	21848.0
37	23116.0
38	23610.5
39	24105.0
40	26181.0
41	28257.0
42	29443.5
43	30630.0
44	30862.5
45	31095.0
46	31919.0
47	32743.0
48	31989.0
49	31235.0
50	29624.0
51	28013.0
52	26122.5
53	24232.0
54	21835.5
55	19439.0
56	18828.5
57	18218.0
58	17367.0
59	16516.0
60	16723.5
61	16931.0
62	15455.0
63	13979.0
64	11501.0
65	9023.0
66	7337.5
67	5652.0
68	4737.5
69	3823.0
70	3203.5
71	2584.0
72	2141.5
73	1699.0
74	1397.0
75	777.5
76	460.0
77	359.5
78	259.0
79	192.5
80	126.0
81	84.0
82	42.0
83	26.5
84	11.0
85	11.0
86	11.0
87	15.5
88	20.0
89	10.0
90	0.0
91	0.5
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	530437.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.240458722817504
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.64244549694696	22.5722179202058
2	7.38100752477925	4.464101067717846
3	3.043056984907297	2.7607031742981176
4	1.8369098642364154	2.221959877079105
5	1.3344783320259033	2.017761845806184
6	1.0932422492044638	1.983608826666465
7	0.931643374811391	1.9721326114259186
8	0.8219915692789759	1.9885921697027884
9	0.7590231488316784	2.06578873817166
>10	7.8966426747362375	48.08468104082462
>50	0.2152590845752192	3.9960988170330456
>100	0.03993212003714211	2.230600132913011
>500	0.0037436362534820733	0.8092576526868469
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	6.239393755803455E-4	2.8324961254686283
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15012	2.830119316714332	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	902	0.17004846946951288	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	823	0.15515508910577505	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	756	0.1425239943669088	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	631	0.11895851910782997	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	615	0.11594213827466786	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	562	0.10595037676481844	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.540952082905227E-4	0.0	0.0	0.02469661807151462	0.0
2	7.540952082905227E-4	0.0	0.0	0.12499128077415414	0.0
3	7.540952082905227E-4	0.0	0.0	0.174573040719256	0.0
4	7.540952082905227E-4	0.0	0.0	0.23753999061151465	0.0
5	7.540952082905227E-4	0.0	0.0	0.4458587919017716	0.0
6	7.540952082905227E-4	0.0	0.0	0.5570878351246237	0.0
7	7.540952082905227E-4	0.0	0.0	0.6713332591806378	0.0
8	7.540952082905227E-4	0.0	0.0	0.8344063479734634	0.0
9	7.540952082905227E-4	0.0	0.0	0.9126437258336051	0.0
10	7.540952082905227E-4	0.0	0.0	1.1055035753539062	0.0
11	7.540952082905227E-4	0.0	0.0	1.4540840853862005	0.0
12	7.540952082905227E-4	0.0	0.0	1.707460075371816	0.0
13	7.540952082905227E-4	0.0	0.0	1.795312167137662	0.0
14	0.0011311428124357841	0.0	0.0	1.8313202133335345	0.0
15	0.0011311428124357841	0.0	0.0	1.9080494007770952	0.0
16	0.0011311428124357841	0.0	0.0	2.0773437750383175	0.0
17	0.0011311428124357841	0.0	0.0	2.2639823390902216	0.0
18	0.0011311428124357841	0.0	0.0	2.468153616734881	0.0
19	0.0011311428124357841	0.0	0.0	2.604833373237538	0.0
20	0.0011311428124357841	0.0	0.0	2.7475458914065194	0.0
21	0.0011311428124357841	0.0	0.0	2.920233694105049	0.0
22	0.0011311428124357841	0.0	0.0	3.1328885428429767	0.0
23	0.0011311428124357841	0.0	0.0	3.3308385350192387	0.0
24	0.0011311428124357841	0.0	0.0	3.482977243291852	0.0
25	0.0011311428124357841	0.0	0.0	3.6155094761489113	0.0
26	0.0011311428124357841	0.0	0.0	3.749172851818406	0.0
27	0.0011311428124357841	0.0	0.0	3.883213275092047	0.0
28	0.0011311428124357841	0.0	0.0	4.022909412427866	0.0
29	0.0011311428124357841	0.0	0.0	4.178441549137786	0.0
30	0.0011311428124357841	0.0	0.0	4.3505637804300985	0.0
31	0.0015081904165810454	0.0	0.0	4.517595869066449	0.0
32	0.0015081904165810454	0.0	0.0	4.667849339318336	0.0
33	0.0015081904165810454	0.0	0.0	4.823381476028256	0.0
34	0.0015081904165810454	0.0	0.0	4.979667707946467	0.0
35	0.0015081904165810454	0.0	0.0	5.158953843717539	0.0
36	0.0015081904165810454	0.0	0.0	5.3363547414678845	0.0
37	0.0015081904165810454	0.0	0.0	5.509419591770559	0.0
38	0.0015081904165810454	0.0	0.0	5.679656585042144	0.0
39	0.001696714218653676	0.0	0.0	5.861582054042233	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	35	1.2102646E-7	45.000004	1
TAACGCG	25	3.8879545E-5	45.000004	1
GTCTGCG	20	7.0298475E-4	45.0	1
TAGCGCG	20	7.0298475E-4	45.0	1
TCTAGCG	20	7.0298475E-4	45.0	1
ATCGTCC	20	7.0298475E-4	45.0	23
TGCGACG	20	7.0298475E-4	45.0	1
ATGCCGG	20	7.0298475E-4	45.0	2
TTACGAG	30	2.1632823E-6	44.999996	1
CGTTTTT	7050	0.0	43.276596	1
CGTAAGG	90	0.0	42.5	2
TACGAAT	80	0.0	42.1875	12
CGAATAT	80	0.0	42.1875	14
CGTTATT	450	0.0	42.0	1
TAAACGG	55	6.002665E-11	40.909092	2
TCGACGG	55	6.002665E-11	40.909092	2
AAGTACG	40	3.4535333E-7	39.375	1
CCTCGTG	40	3.4535333E-7	39.375	15
GTCGATG	35	6.2419276E-6	38.57143	1
GGCCGAT	105	0.0	38.57143	8
>>END_MODULE