##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550384_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	445987
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.168078890191865	33.0	31.0	34.0	30.0	34.0
2	32.261633186617544	34.0	31.0	34.0	30.0	34.0
3	32.08869541040434	33.0	31.0	34.0	30.0	34.0
4	35.872186857464456	37.0	35.0	37.0	35.0	37.0
5	33.76160740111259	37.0	35.0	37.0	32.0	37.0
6	34.774623475572156	37.0	35.0	37.0	32.0	37.0
7	35.863769571758816	37.0	35.0	37.0	33.0	37.0
8	35.88479933271598	37.0	35.0	37.0	35.0	37.0
9	37.86101388605498	39.0	38.0	39.0	35.0	39.0
10	37.51658456412407	39.0	37.0	39.0	35.0	39.0
11	37.44046575348609	39.0	37.0	39.0	35.0	39.0
12	37.46823786343548	39.0	37.0	39.0	35.0	39.0
13	37.48806355342196	39.0	37.0	39.0	35.0	39.0
14	38.74325260601766	40.0	38.0	41.0	35.0	41.0
15	38.85830977136105	40.0	38.0	41.0	35.0	41.0
16	38.80516472453233	40.0	38.0	41.0	35.0	41.0
17	38.78643772127887	40.0	38.0	41.0	35.0	41.0
18	38.49051205528412	40.0	38.0	41.0	35.0	41.0
19	38.150255500720874	40.0	37.0	41.0	34.0	41.0
20	37.890869016361464	39.0	35.0	41.0	34.0	41.0
21	37.77370192404712	39.0	35.0	41.0	34.0	41.0
22	37.77219291145258	39.0	35.0	41.0	34.0	41.0
23	37.78772699652681	39.0	35.0	41.0	34.0	41.0
24	37.695946294398716	39.0	35.0	41.0	34.0	41.0
25	37.49644496364244	39.0	35.0	41.0	33.0	41.0
26	37.546036095222505	39.0	35.0	41.0	34.0	41.0
27	37.526291125077634	39.0	35.0	41.0	34.0	41.0
28	37.414180233953005	39.0	35.0	41.0	33.0	41.0
29	37.34502350965387	39.0	35.0	41.0	33.0	41.0
30	37.06212064477216	39.0	35.0	41.0	33.0	41.0
31	36.78880998773507	39.0	35.0	41.0	32.0	41.0
32	36.34758636462498	39.0	35.0	41.0	30.0	41.0
33	35.83148836176839	39.0	35.0	41.0	25.0	41.0
34	35.376212759564744	39.0	35.0	41.0	21.0	41.0
35	35.081154831867295	39.0	35.0	41.0	18.0	41.0
36	34.85333429001294	39.0	35.0	41.0	18.0	41.0
37	34.73262897797469	39.0	35.0	41.0	17.0	41.0
38	34.60319022751784	39.0	35.0	41.0	16.0	41.0
39	34.5007275996834	39.0	35.0	41.0	15.0	41.0
40	34.39881207299764	39.0	35.0	41.0	15.0	41.0
41	34.26551446566828	39.0	34.0	41.0	15.0	41.0
42	34.20838948220464	39.0	34.0	41.0	15.0	41.0
43	34.12937148392218	39.0	34.0	41.0	13.0	41.0
44	33.96495189321662	39.0	34.0	41.0	12.0	41.0
45	33.939547565287775	39.0	34.0	41.0	12.0	41.0
46	33.84837226197176	38.0	34.0	41.0	12.0	41.0
47	33.84894178529868	38.0	34.0	41.0	12.0	41.0
48	33.77998461838574	38.0	33.0	41.0	12.0	41.0
49	33.72500095294257	38.0	33.0	41.0	12.0	41.0
50	33.6600573559319	38.0	33.0	41.0	12.0	41.0
51	32.81532645570387	37.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	6.0
10	9.0
11	11.0
12	11.0
13	6.0
14	14.0
15	35.0
16	32.0
17	82.0
18	189.0
19	339.0
20	610.0
21	909.0
22	1471.0
23	2276.0
24	4046.0
25	7293.0
26	11335.0
27	13747.0
28	12800.0
29	11100.0
30	9820.0
31	9801.0
32	10501.0
33	13082.0
34	21295.0
35	29958.0
36	28173.0
37	38792.0
38	72105.0
39	146017.0
40	114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.99042348768013	17.921374389836476	21.860502660391447	27.22769946209194
2	42.48240419563799	21.86565976138318	21.451746351351048	14.200189691627783
3	26.245159612275692	20.428173915383184	39.76169708982549	13.564969382515635
4	24.834131936581112	21.24949830376222	38.7740001390175	15.142369620639167
5	20.44700854509212	30.669279597835814	34.55773374560245	14.325978111469617
6	22.90335816963275	29.291885189478617	35.9198810727667	11.884875568121942
7	67.93740624726729	5.086471130324426	23.650465148087278	3.3256574743210003
8	68.70200252473727	5.771020231531413	22.496395634850344	3.0305816088809765
9	63.09466419424782	8.092612564940234	24.12290044328646	4.689822797525489
10	31.314814108931426	29.358479058806648	30.216575819474556	9.110131012787368
11	22.05804205055304	21.78695791581369	41.23147087246938	14.92352916116389
12	21.9199214326875	19.477697780428578	42.95371838192593	15.648662404957992
13	19.52814768143466	21.420355301836153	43.142737344362054	15.90875967236713
14	15.4497776841029	24.602735057299878	41.68664109043537	18.26084616816185
15	15.815259189169192	24.13388731061668	44.14411182388724	15.906741676326888
16	17.70500036996594	23.460549298522153	42.78174027494075	16.052710056571158
17	18.220037803792486	23.5968761421297	40.7718162188584	17.41126983521941
18	17.150948346028024	24.276716585909455	42.60393240161709	15.968402666445433
19	18.950328148578322	23.42624336583802	41.07608517737064	16.54734330821302
20	19.121185146652255	23.988591595719157	42.34719846094169	14.543024796686899
21	19.483975990331555	23.070851840972942	42.13216977176465	15.313002396930852
22	18.058149677008522	21.77529838313673	42.33979914212746	17.826752797727288
23	17.87518470269313	22.55828084675114	43.37973976819952	16.18679468235621
24	17.06148385491057	22.325538636776407	43.72078109900065	16.89219640931238
25	17.05520564500759	25.53639455858579	40.70320435349012	16.705195442916498
26	16.387024733904802	25.613302629897284	40.873612907999565	17.126059728198356
27	17.286826746071075	24.123124665068712	42.111317146015466	16.478731442844747
28	15.288786444447933	25.33347384565021	42.60258707092359	16.775152638978266
29	16.539271324052045	23.59149481935572	42.24315955397803	17.626074302614203
30	16.416846230943953	24.445107144378646	41.48753214779803	17.65051447687937
31	17.34826351440737	25.806357584413895	39.742414016552054	17.10296488462668
32	17.337500868859408	26.535975263852983	38.480045382488726	17.646478484798884
33	17.45118131245978	26.254576927130163	37.56992916833899	18.72431259207107
34	16.88210642911116	26.99675102637521	37.54324677625133	18.577895768262305
35	17.77204268285847	25.779675192326234	36.23827600356064	20.210006121254658
36	17.89334666705532	27.81403942267375	34.782628193198455	19.50998571707247
37	17.80881505514735	29.450634211311094	35.62345987663317	17.117090856908384
38	17.215075775751313	28.593658559554424	34.84271963084126	19.348546033853005
39	18.31062340382119	27.451248578994452	34.57051438719066	19.6676136299937
40	18.24627175231565	27.435328832454758	35.11671864874985	19.20168076647974
41	16.490839419086207	27.608876491915684	34.94048032790194	20.959803761096175
42	17.554547554076688	27.85507200882537	34.51176828024135	20.07861215685659
43	18.17609033447163	27.416494202745824	34.35705525048937	20.050360212293185
44	17.781011554148442	28.390737846618848	34.111756620708675	19.71649397852404
45	17.939984797763163	27.692286994912408	34.03148522266344	20.336242984660988
46	17.781908441277437	28.219656626762664	34.42521867229314	19.57321625966676
47	17.852314080903703	27.06984732738847	35.71987524300035	19.357963348707475
48	17.516205629312065	26.262873133073384	35.66763156773628	20.553289669878268
49	16.987490666768316	27.091821062048894	36.19365586889304	19.727032402289755
50	16.5085529398839	26.429021473720088	36.14948417778994	20.91294140860608
51	17.209918674759578	26.220719438010526	34.43396332180086	22.13539856542904
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	367.0
1	1013.5
2	1660.0
3	14944.0
4	28228.0
5	18663.0
6	9098.0
7	8408.5
8	7719.0
9	7270.0
10	6821.0
11	6567.5
12	6314.0
13	6038.5
14	5763.0
15	5265.5
16	4768.0
17	4502.5
18	4237.0
19	3922.0
20	3607.0
21	3527.5
22	3448.0
23	3585.0
24	3722.0
25	4698.0
26	5995.0
27	6316.0
28	7749.0
29	9182.0
30	10512.0
31	11842.0
32	12756.5
33	13671.0
34	15145.5
35	16620.0
36	17623.5
37	18627.0
38	20127.0
39	21627.0
40	23327.5
41	25028.0
42	25164.0
43	25300.0
44	26593.5
45	27887.0
46	29668.5
47	31450.0
48	31455.5
49	31461.0
50	29048.0
51	26635.0
52	22856.0
53	19077.0
54	17074.5
55	15072.0
56	13385.0
57	11698.0
58	11182.5
59	10667.0
60	10342.5
61	10018.0
62	9244.5
63	8471.0
64	7073.0
65	5675.0
66	4548.0
67	3421.0
68	2743.0
69	2065.0
70	1616.0
71	1167.0
72	960.5
73	754.0
74	573.5
75	311.5
76	230.0
77	164.0
78	98.0
79	68.0
80	38.0
81	28.5
82	19.0
83	28.5
84	38.0
85	21.5
86	5.0
87	5.0
88	5.0
89	3.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	445987.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.01957885860601
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.47194743753025	25.580910777433097
2	7.8522814553899405	5.1855805347243535
3	2.843031827795883	2.816271409062988
4	1.6158351183643067	2.134167804533408
5	1.0896427596974694	1.798977251576983
6	0.7758160365309061	1.537027127880205
7	0.7038283565072381	1.6268081144429667
8	0.5887321544214957	1.5551750239614068
9	0.49180247216972195	1.4615199461398969
>10	6.281056505567374	44.34935683292825
>50	0.24652706457431295	5.018378076826437
>100	0.032688671545765255	2.1099359902703076
>500	0.006129125914830984	1.433083455823582
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	6.810139905367761E-4	3.392807654396078
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15088	3.3830582505768105	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	959	0.2150286891770388	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	920	0.20628403966931771	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	845	0.18946740600062334	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCG	696	0.15605836044548382	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	636	0.14260505351052832	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTC	624	0.13991439212353723	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	614	0.13767217430104467	No Hit
GCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	577	0.12937596835782209	Illumina PCR Primer Index 11 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	502	0.1125593346891277	No Hit
CCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	453	0.10157246735891406	Illumina PCR Primer Index 11 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.4844356449851675E-4	0.0	0.0	0.07332052279550749	0.0
2	4.4844356449851675E-4	0.0	0.0	0.4094289743871458	0.0
3	4.4844356449851675E-4	0.0	0.0	0.5347689506644813	0.0
4	4.4844356449851675E-4	0.0	0.0	0.715043263592885	0.0
5	4.4844356449851675E-4	0.0	0.0	1.31214586972266	0.0
6	4.4844356449851675E-4	0.0	0.0	1.5657407054465713	0.0
7	4.4844356449851675E-4	0.0	0.0	1.862161901580091	0.0
8	4.4844356449851675E-4	0.0	0.0	2.261949339330519	0.0
9	4.4844356449851675E-4	0.0	0.0	2.3778720007533853	0.0
10	4.4844356449851675E-4	0.0	0.0	2.86622704249227	0.0
11	4.4844356449851675E-4	0.0	0.0	3.450324785251588	0.0
12	4.4844356449851675E-4	0.0	0.0	3.9826272963113274	0.0
13	4.4844356449851675E-4	0.0	0.0	4.151466298345019	0.0
14	4.4844356449851675E-4	0.0	0.0	4.216714836979553	0.0
15	4.4844356449851675E-4	0.0	0.0	4.361562108312574	0.0
16	4.4844356449851675E-4	0.0	0.0	4.6348884608744205	0.0
17	8.968871289970335E-4	0.0	0.0	4.990504207521744	0.0
18	8.968871289970335E-4	0.0	0.0	5.370560128434237	0.0
19	0.001121108911246292	0.0	0.0	5.638505158222101	0.0
20	0.001121108911246292	0.0	0.0	5.889185110776771	0.0
21	0.001121108911246292	0.0	0.0	6.199956500974244	0.0
22	0.001121108911246292	0.0	0.0	6.5522089208878285	0.0
23	0.001121108911246292	0.0	0.0	6.890111146737461	0.0
24	0.001121108911246292	0.0	0.0	7.161419503259063	0.0
25	0.001121108911246292	0.0	0.0	7.408063463733248	0.0
26	0.001121108911246292	0.0	0.0	7.634527463804999	0.0
27	0.001121108911246292	0.0	0.0	7.87892920645669	0.0
28	0.0013453306934955503	0.0	0.0	8.12131295306814	0.0
29	0.0013453306934955503	0.0	0.0	8.37199290562281	0.0
30	0.0013453306934955503	0.0	0.0	8.6473372542249	0.0
31	0.0013453306934955503	0.0	0.0	8.903398529553552	0.0
32	0.0013453306934955503	0.0	0.0	9.168877119736674	0.0
33	0.0013453306934955503	0.0	0.0	9.417090632686604	0.0
34	0.0013453306934955503	0.0	0.0	9.680327005047232	0.0
35	0.0013453306934955503	0.0	0.0	9.976075535834005	0.0
36	0.0013453306934955503	0.0	0.0	10.245590118097613	0.0
37	0.0013453306934955503	0.0	0.0	10.540665983537636	0.0
38	0.0013453306934955503	0.0	0.0	10.78730994401182	0.0
39	0.0013453306934955503	0.0	0.0	11.05973940944467	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATTA	30	2.162502E-6	45.000004	9
CGGGTCC	20	7.028638E-4	45.0	6
AATCCGA	25	3.8869526E-5	45.0	26
TCGTTCC	20	7.028638E-4	45.0	37
TTGTGCG	50	2.1827873E-11	45.0	1
ATGTGCG	20	7.028638E-4	45.0	1
AGACGCG	20	7.028638E-4	45.0	1
GTTCGCA	25	3.8869526E-5	45.0	42
CGCTAGG	20	7.028638E-4	45.0	2
TCGCTCA	20	7.028638E-4	45.0	39
TCATCGG	20	7.028638E-4	45.0	2
ACGTAGG	35	1.2097007E-7	45.0	2
CGCCGGT	35	1.2097007E-7	45.0	28
TTCGCAC	25	3.8869526E-5	45.0	43
TCGACAG	20	7.028638E-4	45.0	1
ATTTGCG	20	7.028638E-4	45.0	1
TATCGCT	20	7.028638E-4	45.0	37
ATCGCTC	20	7.028638E-4	45.0	38
CTTACGT	20	7.028638E-4	45.0	22
CGTTTTT	7575	0.0	43.81188	1
>>END_MODULE