SRR3550374_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550374_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	305882
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGC	3223	1.0536742927011071	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCG	3016	0.9860011376936204	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTC	2732	0.8931548767171655	No Hit
GCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	1871	0.6116737827005185	TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	1186	0.38773121661294224	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGCT	777	0.2540195238686814	TruSeq Adapter, Index 22 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCT	626	0.2046540822931719	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCTGTTTT	556	0.1817694405032006	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTA	468	0.15300017653866524	No Hit
GAACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCT	466	0.15234632963038033	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTC	445	0.14548093709338897	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGC	426	0.13926939146468245	No Hit
TCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	360	0.11769244349128095	TruSeq Adapter, Index 20 (95% over 21bp)
GAATAATACCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCG	346	0.11311551513328669	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	343	0.11213474477085934	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAGG	25	3.88407E-5	45.000004	2
AACGGGC	35	1.2080272E-7	45.000004	4
AGCGCAG	25	3.88407E-5	45.000004	1
AATTACC	25	3.88407E-5	45.000004	24
CGATAGC	25	3.88407E-5	45.000004	10
TTTAGCG	20	7.02516E-4	45.0	1
TAATACG	80	0.0	45.0	4
CAGCGAC	20	7.02516E-4	45.0	35
TAGACCA	20	7.02516E-4	45.0	26
CCAGCGA	20	7.02516E-4	45.0	34
CGTTATT	40	6.7866495E-9	45.0	1
TACGCAG	20	7.02516E-4	45.0	1
TGTTACG	20	7.02516E-4	45.0	1
ATAACGG	20	7.02516E-4	45.0	2
TAGGCCG	20	7.02516E-4	45.0	4
AACAGCG	20	7.02516E-4	45.0	1
AATACGG	80	0.0	45.0	5
TAGTAAC	20	7.02516E-4	45.0	40
CGGGTAT	30	2.160259E-6	44.999996	6
GTTACGG	30	2.160259E-6	44.999996	2