##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550374_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	305882
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.566169307118432	31.0	31.0	34.0	30.0	34.0
2	31.63490169411734	31.0	31.0	34.0	30.0	34.0
3	31.653036138118622	31.0	31.0	34.0	30.0	34.0
4	35.45671533467154	37.0	35.0	37.0	33.0	37.0
5	31.156171334043847	35.0	35.0	37.0	0.0	37.0
6	33.1628601879156	36.0	35.0	37.0	17.0	37.0
7	35.07213892939107	37.0	35.0	37.0	32.0	37.0
8	35.27288954564178	37.0	35.0	37.0	33.0	37.0
9	37.211872552160635	39.0	37.0	39.0	34.0	39.0
10	36.63244322974219	39.0	35.0	39.0	32.0	39.0
11	36.65042075048548	39.0	35.0	39.0	32.0	39.0
12	36.729807572854895	39.0	35.0	39.0	33.0	39.0
13	36.59421280101477	39.0	35.0	39.0	32.0	39.0
14	37.629242649126134	39.0	36.0	41.0	33.0	41.0
15	37.7143637088812	39.0	36.0	41.0	33.0	41.0
16	37.818819021714255	39.0	36.0	41.0	33.0	41.0
17	37.729977573051045	39.0	36.0	41.0	33.0	41.0
18	37.772180121746295	39.0	36.0	41.0	33.0	41.0
19	37.70979004975775	39.0	36.0	41.0	33.0	41.0
20	37.62324360374262	39.0	36.0	41.0	33.0	41.0
21	37.49884596020688	39.0	36.0	41.0	32.0	41.0
22	37.54930332611922	39.0	36.0	41.0	33.0	41.0
23	37.457078219705636	39.0	36.0	41.0	32.0	41.0
24	37.38199697922728	39.0	35.0	41.0	32.0	41.0
25	37.203405234698344	39.0	35.0	40.0	32.0	41.0
26	37.28539109852819	39.0	35.0	41.0	32.0	41.0
27	37.22015025401952	39.0	35.0	41.0	32.0	41.0
28	37.15699845038283	39.0	35.0	41.0	32.0	41.0
29	37.20341504240197	39.0	35.0	41.0	32.0	41.0
30	37.06964450343597	39.0	35.0	41.0	32.0	41.0
31	37.12868361001955	39.0	35.0	41.0	32.0	41.0
32	37.042866203307156	39.0	35.0	41.0	31.0	41.0
33	37.02496060572378	39.0	35.0	41.0	31.0	41.0
34	36.95601898771422	39.0	35.0	41.0	31.0	41.0
35	36.899977769205115	39.0	35.0	41.0	31.0	41.0
36	36.81481094016647	39.0	35.0	41.0	31.0	41.0
37	36.783707442739356	39.0	35.0	41.0	31.0	41.0
38	36.75095298186883	39.0	35.0	41.0	31.0	41.0
39	36.63978919975677	39.0	35.0	40.0	31.0	41.0
40	36.58137124773606	39.0	35.0	40.0	31.0	41.0
41	36.47968824579413	39.0	35.0	40.0	30.0	41.0
42	36.55132698230037	39.0	35.0	40.0	31.0	41.0
43	36.50305346506169	39.0	35.0	40.0	31.0	41.0
44	36.4316076133934	39.0	35.0	40.0	31.0	41.0
45	36.361024839644045	39.0	35.0	40.0	30.0	41.0
46	36.27695320417678	39.0	35.0	40.0	30.0	41.0
47	36.14556920642601	38.0	35.0	40.0	30.0	41.0
48	36.14394439685892	38.0	35.0	40.0	30.0	41.0
49	36.14853767138962	38.0	35.0	40.0	30.0	41.0
50	36.040816393249685	38.0	35.0	40.0	30.0	41.0
51	35.0492150567866	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	14.0
10	11.0
11	9.0
12	4.0
13	2.0
14	6.0
15	12.0
16	22.0
17	29.0
18	75.0
19	172.0
20	327.0
21	557.0
22	958.0
23	1256.0
24	1675.0
25	2054.0
26	2498.0
27	2934.0
28	3406.0
29	4228.0
30	5542.0
31	7282.0
32	9830.0
33	13776.0
34	21429.0
35	26408.0
36	29117.0
37	42244.0
38	61717.0
39	68263.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.679229245264516	18.013809246702976	20.16071557005643	13.146245937976081
2	30.95540110238589	28.10757089335103	22.91341105393583	18.023616950327252
3	31.052824291720338	28.406705853891367	25.055086602023003	15.485383252365292
4	28.366821192485993	24.398297382650828	28.411282782249366	18.823598642613817
5	24.64708613125323	37.07377354666178	20.67823539796392	17.600904924121068
6	24.701355424640877	37.22579295283802	24.07725855068294	13.995593071838162
7	74.96845188667525	5.986622292256491	13.869073695085032	5.175852125983222
8	74.022335410387	9.253568369501965	9.940761470109388	6.783334750001635
9	69.23879142937473	6.79477707089662	12.07589854911371	11.890532950614944
10	39.335756925873376	23.1896613726862	20.34411962783034	17.130462073610083
11	31.477497858651375	24.71116312826515	24.403201234462962	19.408137778620514
12	26.38468429002033	23.38581544517167	28.51720598139152	21.71229428341648
13	25.237509889434488	25.3885485252483	32.25263336842312	17.121308216894096
14	22.09708318894214	29.803649773442046	27.153935177617512	20.9453318599983
15	20.265984922290293	25.439875507548663	35.20638677660013	19.087752793560917
16	22.136640926893378	25.280009938473007	29.4551493713262	23.128199763307418
17	22.118660136915544	26.572992199606382	28.195840225969494	23.11250743750858
18	22.64566074499317	24.922028756187025	30.112919361060804	22.319391137759006
19	22.44296820342485	27.67112808207086	26.94339647314978	22.94250724135451
20	24.952432637422273	26.50826135568618	29.83634211885629	18.702963888035256
21	22.850968674194625	30.119784753597795	28.38414813555554	18.64509843665204
22	21.137235927579916	25.32839460968609	28.498244421051254	25.036125041682737
23	20.928658763837035	28.541725240452198	28.30666727692378	22.222948718786984
24	22.229487187869832	25.08189432526268	28.53812908245663	24.150489404410852
25	19.959003798850535	30.389169679811168	27.10097357804644	22.550852943291858
26	20.87471639390353	26.145703245042206	29.763438188582526	23.216142172471738
27	22.7698916575673	26.8364925036452	26.562203725619682	23.831412113167826
28	19.314637670735774	26.02931849536749	30.62291994952302	24.033123884373715
29	23.371430813189402	23.901373732354305	28.014397708920434	24.712797745535863
30	22.454083600865694	26.485049790442066	26.912665668460388	24.148200940231852
31	22.953622638795352	25.073394315454976	25.45131782844365	26.52166521730602
32	23.977219973715354	26.073453161676724	28.423705873506776	21.525620991101142
33	22.787545524090987	23.64016189249449	27.593974146893245	25.978318436521274
34	23.260276838780968	22.600218384867368	30.872362545033706	23.26714223131796
35	21.170582119902445	23.278911475667087	30.892304875736393	24.65820152869407
36	23.654546524476757	24.45877822166718	28.667917693751182	23.218757560104876
37	19.554272562622188	23.55058486605946	33.16409595857226	23.73104661274609
38	20.870466388999677	25.361413878554472	28.846744823167104	24.92137490927874
39	22.399814307478046	23.01344963090342	33.00226884877175	21.584467212846782
40	24.06745084705867	22.55771833582885	31.36699773115123	22.007833085961252
41	19.624561105262813	23.97623920335293	30.88184332520384	25.517356366180422
42	21.45304398428152	22.25171798275152	32.64722997757305	23.64800805539391
43	23.599296460726684	22.14350631943037	29.362303110349742	24.894894109493205
44	21.406620853793292	23.22594987609601	29.581014901171038	25.78641436893966
45	21.65115959749184	21.72537122158218	29.149149018248867	27.474320162677113
46	24.688932333383462	23.34364231958729	29.59311106897431	22.374314278054936
47	18.889637180350594	22.815007094238954	35.03279042245049	23.262565302959963
48	21.07871662928842	22.09414087785486	30.785400906231814	26.041741586624905
49	21.548832556345257	20.530465996691536	34.430924343374244	23.489777103588967
50	21.444217051019674	20.8998894998725	31.94663301534579	25.709260433762037
51	21.369678503475196	20.28788879371784	28.68982156517873	29.652611137628238
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	107.0
1	215.0
2	323.0
3	622.0
4	921.0
5	692.5
6	464.0
7	500.0
8	536.0
9	546.0
10	556.0
11	559.5
12	563.0
13	575.0
14	587.0
15	622.5
16	658.0
17	609.0
18	560.0
19	586.0
20	612.0
21	648.5
22	685.0
23	844.5
24	1004.0
25	1116.0
26	1520.0
27	1812.0
28	2147.0
29	2482.0
30	3168.0
31	3854.0
32	4421.0
33	4988.0
34	5839.0
35	6690.0
36	7254.0
37	7818.0
38	9136.0
39	10454.0
40	11987.5
41	13521.0
42	15075.5
43	16630.0
44	19716.5
45	22803.0
46	30828.0
47	38853.0
48	36913.5
49	34974.0
50	35488.5
51	36003.0
52	31161.5
53	26320.0
54	22523.0
55	18726.0
56	16273.5
57	13821.0
58	12080.0
59	10339.0
60	9003.0
61	7667.0
62	6559.5
63	5452.0
64	4773.0
65	4094.0
66	3641.5
67	3189.0
68	2649.0
69	2109.0
70	1754.5
71	1400.0
72	1209.0
73	1018.0
74	875.5
75	653.0
76	573.0
77	462.0
78	351.0
79	290.0
80	229.0
81	169.0
82	109.0
83	74.0
84	39.0
85	29.0
86	19.0
87	11.0
88	3.0
89	3.0
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	305882.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.95539289125759
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.33323110184698	30.03391291761534
2	10.830255551138526	8.871147424192037
3	3.9714681631014477	4.8795911692482274
4	2.1049673642506885	3.4483906170464755
5	1.385285240197765	2.8367450639379803
6	1.0388815540455474	2.552868132804939
7	0.8259458838845553	2.367885672698629
8	0.7464393047059052	2.445657199496599
9	0.7103108562971631	2.618195417511833
>10	4.966533420262258	31.671296456120906
>50	0.056985099806588974	1.5843863761066397
>100	0.02327560414635324	2.0922446647134363
>500	0.002407821118588266	0.643944587301433
>1k	0.00401303519764711	3.953734301205532
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGC	3223	1.0536742927011071	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCG	3016	0.9860011376936204	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTC	2732	0.8931548767171655	No Hit
GCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	1871	0.6116737827005185	TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	1186	0.38773121661294224	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGCT	777	0.2540195238686814	TruSeq Adapter, Index 22 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCT	626	0.2046540822931719	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCTGTTTT	556	0.1817694405032006	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTA	468	0.15300017653866524	No Hit
GAACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCT	466	0.15234632963038033	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTC	445	0.14548093709338897	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGC	426	0.13926939146468245	No Hit
TCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	360	0.11769244349128095	TruSeq Adapter, Index 20 (95% over 21bp)
GAATAATACCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCG	346	0.11311551513328669	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	343	0.11213474477085934	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.269234541424471E-4	0.0	0.0	0.26284645713052746	0.0
2	3.269234541424471E-4	0.0	0.0	1.5113671285005328	0.0
3	3.269234541424471E-4	0.0	0.0	1.9697138112082437	0.0
4	3.269234541424471E-4	0.0	0.0	2.677503089426642	0.0
5	3.269234541424471E-4	0.0	0.0	5.0339673468854	0.0
6	3.269234541424471E-4	0.0	0.0	6.159564799497845	0.0
7	3.269234541424471E-4	0.0	0.0	7.411027781955133	0.0
8	3.269234541424471E-4	0.0	0.0	9.231664498074421	0.0
9	3.269234541424471E-4	0.0	0.0	9.74722278525706	0.0
10	3.269234541424471E-4	0.0	0.0	12.182148671710006	0.0
11	3.269234541424471E-4	0.0	0.0	13.894900647962286	0.0
12	3.269234541424471E-4	0.0	0.0	16.507019046560437	0.0
13	3.269234541424471E-4	0.0	0.0	17.136019772330506	0.0
14	3.269234541424471E-4	0.0	0.0	17.381539286391483	0.0
15	6.538469082848942E-4	0.0	0.0	17.998770767812424	0.0
16	6.538469082848942E-4	0.0	0.0	18.995887302946887	0.0
17	6.538469082848942E-4	0.0	0.0	20.20779254745294	0.0
18	9.807703624273413E-4	0.0	0.0	21.472005544621783	0.0
19	9.807703624273413E-4	0.0	0.0	22.392621991486912	0.0
20	9.807703624273413E-4	0.0	0.0	23.066084307020354	0.0
21	9.807703624273413E-4	0.0	0.0	23.921316063056995	0.0
22	9.807703624273413E-4	0.0	0.0	24.787009369626197	0.0
23	9.807703624273413E-4	0.0	0.0	25.664144997090382	0.0
24	9.807703624273413E-4	0.0	0.0	26.38533813692862	0.0
25	9.807703624273413E-4	0.0	0.0	26.99831961344571	0.0
26	9.807703624273413E-4	0.0	0.0	27.566839500199425	0.0
27	9.807703624273413E-4	0.0	0.0	28.20597485304791	0.0
28	9.807703624273413E-4	0.0	0.0	28.74212931784152	0.0
29	9.807703624273413E-4	0.0	0.0	29.352822330179613	0.0
30	9.807703624273413E-4	0.0	0.0	30.05113082822788	0.0
31	9.807703624273413E-4	0.0	0.0	30.704977736512774	0.0
32	9.807703624273413E-4	0.0	0.0	31.277093781262057	0.0
33	9.807703624273413E-4	0.0	0.0	31.853132907461045	0.0
34	9.807703624273413E-4	0.0	0.0	32.440614354555024	0.0
35	0.0013076938165697883	0.0	0.0	33.01926886838716	0.0
36	0.0013076938165697883	0.0	0.0	33.556077180089055	0.0
37	0.0013076938165697883	0.0	0.0	34.09484703251581	0.0
38	0.0013076938165697883	0.0	0.0	34.651597674920396	0.0
39	0.0013076938165697883	0.0	0.0	35.24463682073479	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAGG	25	3.88407E-5	45.000004	2
AACGGGC	35	1.2080272E-7	45.000004	4
AGCGCAG	25	3.88407E-5	45.000004	1
AATTACC	25	3.88407E-5	45.000004	24
CGATAGC	25	3.88407E-5	45.000004	10
TTTAGCG	20	7.02516E-4	45.0	1
TAATACG	80	0.0	45.0	4
CAGCGAC	20	7.02516E-4	45.0	35
TAGACCA	20	7.02516E-4	45.0	26
CCAGCGA	20	7.02516E-4	45.0	34
CGTTATT	40	6.7866495E-9	45.0	1
TACGCAG	20	7.02516E-4	45.0	1
TGTTACG	20	7.02516E-4	45.0	1
ATAACGG	20	7.02516E-4	45.0	2
TAGGCCG	20	7.02516E-4	45.0	4
AACAGCG	20	7.02516E-4	45.0	1
AATACGG	80	0.0	45.0	5
TAGTAAC	20	7.02516E-4	45.0	40
CGGGTAT	30	2.160259E-6	44.999996	6
GTTACGG	30	2.160259E-6	44.999996	2
>>END_MODULE