FastQCFastQC Report
Sat 18 Jun 2016
SRR3550371_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550371_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences846413
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTC72410.8554925314237849Illumina PCR Primer Index 11 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGC63120.7457352379984712No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCG60060.7095826741791537No Hit
GCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC26770.3162758606023301Illumina PCR Primer Index 11 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCT14990.17710030446129726Illumina PCR Primer Index 11 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14970.17686401319450434No Hit
CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC14750.17426480925978216Illumina PCR Primer Index 11 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCT12840.15169899328105782No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTACAGCT11420.13492231333876017No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTC10270.12133556549816697No Hit
CTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGCT10000.11814563339646249Illumina PCR Primer Index 11 (96% over 25bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTA9190.10857583709134903No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCG253.889932E-545.01
GTCGTAC207.0322317E-445.042
TAATACG2500.041.44
GCTCGAA1700.041.0294142
ATGGGCG3150.040.7142875
CGATGAA8950.040.4748619
AATACGG2550.039.7058835
TACGGCT8600.039.5058147
TTTCGCG403.4567893E-739.3751
CCGATGA9250.038.67567418
TACGGGA1400.038.5714264
TATGCGT356.2463387E-638.57142611
GTTAACG356.2463387E-638.5714261
TAGCCGT1000.038.2544
CGTATGG659.094947E-1238.0769272
GCTACGA659.094947E-1238.07692710
CGCTCGA1250.037.80000341
TTTAGCG301.139668E-437.4999961
CCGTATG301.139668E-437.4999961
CCGTAGG301.139668E-437.4999961
Produced by FastQC (version 0.11.5)