##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3550371_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 846413 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.71052311342099 31.0 31.0 34.0 30.0 34.0 2 31.788223952136843 33.0 31.0 34.0 30.0 34.0 3 31.79871292146978 33.0 31.0 34.0 30.0 34.0 4 35.5467874430095 37.0 35.0 37.0 33.0 37.0 5 31.270239233093065 37.0 35.0 37.0 0.0 37.0 6 33.26263183575867 37.0 35.0 37.0 17.0 37.0 7 35.149350258089136 37.0 35.0 37.0 32.0 37.0 8 35.381807699078344 37.0 35.0 37.0 33.0 37.0 9 37.32044758291756 39.0 37.0 39.0 34.0 39.0 10 36.80035750868666 39.0 37.0 39.0 32.0 39.0 11 36.72174222276832 39.0 35.0 39.0 32.0 39.0 12 36.75537237731462 39.0 35.0 39.0 33.0 39.0 13 36.65224541683552 39.0 35.0 39.0 32.0 39.0 14 37.750921831304574 40.0 36.0 41.0 33.0 41.0 15 37.830114849370226 40.0 37.0 41.0 33.0 41.0 16 37.912357206233835 40.0 36.0 41.0 33.0 41.0 17 37.799321371481774 40.0 36.0 41.0 33.0 41.0 18 37.828346209238276 40.0 36.0 41.0 33.0 41.0 19 37.79393629351156 40.0 36.0 41.0 33.0 41.0 20 37.70155467838986 40.0 36.0 41.0 33.0 41.0 21 37.60718939808344 39.0 36.0 41.0 33.0 41.0 22 37.64480224193154 39.0 36.0 41.0 33.0 41.0 23 37.55605833086212 39.0 36.0 41.0 33.0 41.0 24 37.51797763030577 39.0 35.0 41.0 33.0 41.0 25 37.34212730664581 39.0 35.0 41.0 32.0 41.0 26 37.38174980771798 39.0 35.0 41.0 32.0 41.0 27 37.30358701957555 39.0 35.0 41.0 32.0 41.0 28 37.22037823143076 39.0 35.0 41.0 32.0 41.0 29 37.243524142469454 39.0 35.0 41.0 32.0 41.0 30 37.07250361230274 39.0 35.0 41.0 32.0 41.0 31 37.12886262380186 39.0 35.0 41.0 32.0 41.0 32 37.04387692533078 39.0 35.0 41.0 32.0 41.0 33 36.9960657504079 39.0 35.0 41.0 32.0 41.0 34 36.8699913635542 39.0 35.0 41.0 31.0 41.0 35 36.8154139882067 39.0 35.0 41.0 31.0 41.0 36 36.70166573528525 39.0 35.0 41.0 31.0 41.0 37 36.65273099538878 39.0 35.0 41.0 31.0 41.0 38 36.615167772706705 39.0 35.0 41.0 31.0 41.0 39 36.51983842403177 39.0 35.0 41.0 31.0 41.0 40 36.44988321304139 39.0 35.0 41.0 30.0 41.0 41 36.313667205016934 39.0 35.0 41.0 30.0 41.0 42 36.322954633258234 39.0 35.0 41.0 30.0 41.0 43 36.23196949952328 39.0 35.0 40.0 30.0 41.0 44 36.182256179902716 38.0 35.0 40.0 30.0 41.0 45 36.07957108409252 38.0 35.0 40.0 30.0 41.0 46 35.95527833339044 38.0 35.0 40.0 30.0 41.0 47 35.80989540567075 38.0 35.0 40.0 29.0 41.0 48 35.7764790947209 38.0 35.0 40.0 29.0 41.0 49 35.74730775637898 38.0 35.0 40.0 29.0 41.0 50 35.63868820540327 38.0 35.0 40.0 29.0 41.0 51 34.60978033182383 36.0 33.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 19.0 9 27.0 10 26.0 11 19.0 12 18.0 13 20.0 14 25.0 15 43.0 16 74.0 17 118.0 18 275.0 19 557.0 20 1094.0 21 2069.0 22 3059.0 23 3967.0 24 5017.0 25 6115.0 26 7350.0 27 8330.0 28 9790.0 29 11842.0 30 14930.0 31 19934.0 32 26072.0 33 38329.0 34 60529.0 35 71351.0 36 78014.0 37 113645.0 38 165030.0 39 198674.0 40 49.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.03238962539564 18.36148546867782 21.455365170430984 15.150759735495555 2 30.24669989709515 27.948531036267166 24.303856391619693 17.500912675017986 3 31.609391632689952 27.814553887995576 24.595794251742355 15.980260227572119 4 28.061714553060973 24.349224314843937 28.920987744753447 18.66807338734164 5 25.367285237821253 36.8412347163855 20.831083643564074 16.960396402229172 6 26.127316097460696 36.05166744839694 23.242081584285685 14.578934869856678 7 77.96241314819125 5.565368206773762 11.66392765706576 4.8082909879692295 8 78.6015810248661 7.999050109107493 8.480257273931285 4.9191115920951125 9 72.78160897812297 6.392860223082584 10.275125736490342 10.5504050623041 10 42.747807512408244 23.061673202089285 17.795922321608952 16.394596963893513 11 32.65462605134846 23.845923916575003 24.134199262062374 19.365250770014168 12 27.551443562421657 22.336849741201988 27.668644030750944 22.44306266562541 13 26.01200595926575 22.638239251996367 31.403818230580104 19.94593655815778 14 21.89262215963129 27.341262480609345 28.451240706369113 22.31487465339025 15 21.137080834060914 24.358912256782446 33.3143512682343 21.18965564092234 16 24.725636302845064 24.598747892577265 27.762215372400945 22.91340043217673 17 23.670950233514844 25.28422885754354 28.16745489495081 22.877366013990805 18 24.484264773816093 24.662782825878146 27.85224234504905 23.00071005525671 19 24.581026047567793 26.292601838582346 25.706363205669103 23.42000890818076 20 27.432943492125002 25.810095071791196 27.138642719334417 19.61831871674939 21 25.264616682399726 28.790554965483757 26.550868193187014 19.393960158929506 22 23.93429685035556 23.54288036691308 28.185294885593677 24.337527897137686 23 24.225289545411048 26.45977790983834 27.30451918862305 22.010413356127565 24 24.45862717136906 24.57984459123383 27.145613311704807 23.8159149256923 25 23.264883691531203 27.9809029398178 25.40485554924133 23.349357819409676 26 22.693295117159117 25.080663931201435 28.182104953491972 24.043935998147475 27 24.642816213834145 25.156986010375547 26.456942414636824 23.74325536115348 28 21.145941756565648 27.26836662480373 28.4615193764746 23.124172242156014 29 25.465227967906923 24.744303312921705 26.904005491409038 22.886463227762334 30 24.213947564604986 25.51130476493154 28.502397765629784 21.772349904833693 31 24.850161800444937 25.297106731583753 26.4178362099826 23.43489525798871 32 26.808189382724507 26.543779455183227 25.844239159842775 20.803792002249494 33 23.969504249107704 24.95129446263231 25.17730705931974 25.901894228940247 34 23.51015402646226 26.853439160315357 27.281598935744135 22.35480787747825 35 24.40144468480517 25.573567513731476 27.070118251964466 22.954869549498884 36 23.807881022621345 27.885323122400056 25.459202540603698 22.847593314374897 37 22.99811085132199 27.785135625279857 27.322713616166105 21.89403990723205 38 22.94837153966208 26.635224175432086 25.14080005860023 25.2756042263056 39 23.692452738793 27.295658266118313 24.966417103707055 24.04547189138163 40 24.700943865465206 23.574661542296727 27.068700504363708 24.65569408787436 41 21.427600946582814 26.55169521262079 25.77122515840376 26.249478682392642 42 24.314253207358583 24.66396428221211 27.433179783391797 23.58860272703751 43 23.927444403618566 23.51570687123189 26.82827414040191 25.728574584747637 44 22.870631712887207 24.04842553221654 28.151505234442286 24.929437520453963 45 22.81510326519087 23.37416840242293 27.212956322740787 26.59777200964541 46 24.760371119063628 25.36645821838748 25.98896756075344 23.88420310179546 47 21.394402023598406 25.15970335994367 30.11272274882357 23.33317186763436 48 22.63611263059523 24.60028378581142 27.923720453253907 24.839883130339445 49 22.885399917061765 23.208055641867503 29.737255925889606 24.169288515181123 50 21.96811721937163 23.840843654338958 28.673590788421254 25.517448337868153 51 21.629275542790577 22.94163723855848 27.634972525232953 27.79411469341799 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 258.0 1 618.5 2 979.0 3 2394.0 4 3809.0 5 2756.5 6 1704.0 7 1681.0 8 1658.0 9 1665.5 10 1673.0 11 1670.0 12 1667.0 13 1684.5 14 1702.0 15 1635.5 16 1569.0 17 1667.0 18 1765.0 19 1802.5 20 1840.0 21 2035.0 22 2230.0 23 2457.0 24 2684.0 25 3309.0 26 4589.5 27 5245.0 28 6322.0 29 7399.0 30 8824.0 31 10249.0 32 12367.5 33 14486.0 34 16246.0 35 18006.0 36 20564.5 37 23123.0 38 24255.0 39 25387.0 40 28439.5 41 31492.0 42 36255.5 43 41019.0 44 46736.0 45 52453.0 46 70381.5 47 88310.0 48 81939.0 49 75568.0 50 76464.0 51 77360.0 52 70551.5 53 63743.0 54 58562.5 55 53382.0 56 50167.0 57 46952.0 58 44006.5 59 41061.0 60 38703.5 61 36346.0 62 33517.0 63 30688.0 64 26925.0 65 23162.0 66 19384.5 67 15607.0 68 13737.5 69 11868.0 70 10405.5 71 8943.0 72 7731.5 73 6520.0 74 5474.5 75 3713.5 76 2998.0 77 2350.0 78 1702.0 79 1228.0 80 754.0 81 553.0 82 352.0 83 263.0 84 174.0 85 145.0 86 116.0 87 68.5 88 21.0 89 18.0 90 15.0 91 9.5 92 4.0 93 5.0 94 6.0 95 3.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 846413.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.689729577258724 #Duplication Level Percentage of deduplicated Percentage of total 1 71.75881181784737 24.89293776746523 2 9.878165625263327 6.853417885195152 3 3.9843463825640018 4.146476956598227 4 2.1979883156236215 3.0499048113183127 5 1.4650427725530009 2.541096879949048 6 1.1291053357951621 2.350101525778445 7 0.9000651580773464 2.185611185493104 8 0.8401427261284237 2.3315459180556766 9 0.7171572656031735 2.239019244732636 >10 7.043449565060084 42.33751019281779 >50 0.05293778122568305 1.2722085238749992 >100 0.02698117798377289 1.552288088538546 >500 0.002390737289699678 0.6746090960261384 >1k 0.002390737289699678 1.2559766468164897 >5k 0.0010246016955855763 2.3172952773402247 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTC 7241 0.8554925314237849 Illumina PCR Primer Index 11 (95% over 21bp) GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGC 6312 0.7457352379984712 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCG 6006 0.7095826741791537 No Hit GCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC 2677 0.3162758606023301 Illumina PCR Primer Index 11 (95% over 24bp) GAACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCT 1499 0.17710030446129726 Illumina PCR Primer Index 11 (95% over 22bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1497 0.17686401319450434 No Hit CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC 1475 0.17426480925978216 Illumina PCR Primer Index 11 (95% over 24bp) GAATGACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCT 1284 0.15169899328105782 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTACAGCT 1142 0.13492231333876017 No Hit GAATGATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTC 1027 0.12133556549816697 No Hit CTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGCT 1000 0.11814563339646249 Illumina PCR Primer Index 11 (96% over 25bp) GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTA 919 0.10857583709134903 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.1814563339646248E-4 0.0 0.0 0.12523437140025023 0.0 2 1.1814563339646248E-4 0.0 0.0 0.7123000237472723 0.0 3 1.1814563339646248E-4 0.0 0.0 0.9860434563268758 0.0 4 1.1814563339646248E-4 0.0 0.0 1.4497650674079912 0.0 5 1.1814563339646248E-4 0.0 0.0 3.0758034198435045 0.0 6 1.1814563339646248E-4 0.0 0.0 4.022386234615961 0.0 7 1.1814563339646248E-4 0.0 0.0 4.867481950300858 0.0 8 1.1814563339646248E-4 0.0 0.0 6.0450394783634 0.0 9 1.1814563339646248E-4 0.0 0.0 6.505453011709414 0.0 10 2.3629126679292496E-4 0.0 0.0 8.14141559735023 0.0 11 2.3629126679292496E-4 0.0 0.0 9.088470994656273 0.0 12 2.3629126679292496E-4 0.0 0.0 10.778662426026065 0.0 13 2.3629126679292496E-4 0.0 0.0 11.168070433700805 0.0 14 2.3629126679292496E-4 0.0 0.0 11.35698530150175 0.0 15 2.3629126679292496E-4 0.0 0.0 11.72134643489644 0.0 16 2.3629126679292496E-4 0.0 0.0 12.232680736236329 0.0 17 2.3629126679292496E-4 0.0 0.0 12.826953272220535 0.0 18 2.3629126679292496E-4 0.0 0.0 13.441428711515536 0.0 19 2.3629126679292496E-4 0.0 0.0 14.001675305081562 0.0 20 2.3629126679292496E-4 0.0 0.0 14.400653109061416 0.0 21 2.3629126679292496E-4 0.0 0.0 14.84972466160137 0.0 22 2.3629126679292496E-4 0.0 0.0 15.367911409678253 0.0 23 3.5443690018938746E-4 0.0 0.0 15.869321477812841 0.0 24 3.5443690018938746E-4 0.0 0.0 16.29051066087123 0.0 25 3.5443690018938746E-4 0.0 0.0 16.667040794505755 0.0 26 4.725825335858499E-4 0.0 0.0 17.004110286585863 0.0 27 4.725825335858499E-4 0.0 0.0 17.373787973483395 0.0 28 4.725825335858499E-4 0.0 0.0 17.723735339603717 0.0 29 4.725825335858499E-4 0.0 0.0 18.08596985159727 0.0 30 4.725825335858499E-4 0.0 0.0 18.53610471483779 0.0 31 4.725825335858499E-4 0.0 0.0 18.930711130381976 0.0 32 4.725825335858499E-4 0.0 0.0 19.33169741012957 0.0 33 4.725825335858499E-4 0.0 0.0 19.727012699474134 0.0 34 5.907281669823124E-4 0.0 0.0 20.110986008012638 0.0 35 5.907281669823124E-4 0.0 0.0 20.501811763288135 0.0 36 5.907281669823124E-4 0.0 0.0 20.88070480959059 0.0 37 5.907281669823124E-4 0.0 0.0 21.26243335109456 0.0 38 5.907281669823124E-4 0.0 0.0 21.677597106849728 0.0 39 7.088738003787749E-4 0.0 0.0 22.124542037988547 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATGCG 25 3.889932E-5 45.0 1 GTCGTAC 20 7.0322317E-4 45.0 42 TAATACG 250 0.0 41.4 4 GCTCGAA 170 0.0 41.02941 42 ATGGGCG 315 0.0 40.714287 5 CGATGAA 895 0.0 40.47486 19 AATACGG 255 0.0 39.705883 5 TACGGCT 860 0.0 39.505814 7 TTTCGCG 40 3.4567893E-7 39.375 1 CCGATGA 925 0.0 38.675674 18 TACGGGA 140 0.0 38.571426 4 TATGCGT 35 6.2463387E-6 38.571426 11 GTTAACG 35 6.2463387E-6 38.571426 1 TAGCCGT 100 0.0 38.25 44 CGTATGG 65 9.094947E-12 38.076927 2 GCTACGA 65 9.094947E-12 38.076927 10 CGCTCGA 125 0.0 37.800003 41 TTTAGCG 30 1.139668E-4 37.499996 1 CCGTATG 30 1.139668E-4 37.499996 1 CCGTAGG 30 1.139668E-4 37.499996 1 >>END_MODULE