##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550369_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	643510
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12247828316576	33.0	31.0	34.0	30.0	34.0
2	32.27556215132632	34.0	31.0	34.0	30.0	34.0
3	32.30886388711908	34.0	31.0	34.0	30.0	34.0
4	35.81881400444438	37.0	35.0	37.0	35.0	37.0
5	35.819193174931236	37.0	35.0	37.0	35.0	37.0
6	35.84647946418859	37.0	35.0	37.0	35.0	37.0
7	36.03801805721745	37.0	35.0	37.0	35.0	37.0
8	36.04216406893444	37.0	35.0	37.0	35.0	37.0
9	37.676374881509226	39.0	38.0	39.0	35.0	39.0
10	37.337382480458736	39.0	37.0	39.0	34.0	39.0
11	37.21857779987879	39.0	37.0	39.0	34.0	39.0
12	36.993125203959536	39.0	35.0	39.0	33.0	39.0
13	36.86264859908937	39.0	35.0	39.0	33.0	39.0
14	38.00794393249522	40.0	37.0	41.0	33.0	41.0
15	38.08344858665755	40.0	37.0	41.0	33.0	41.0
16	38.18829855013908	40.0	37.0	41.0	34.0	41.0
17	38.113420148871036	40.0	37.0	41.0	34.0	41.0
18	38.07760252365931	40.0	36.0	41.0	34.0	41.0
19	38.0210765955463	40.0	36.0	41.0	34.0	41.0
20	37.94208015415456	40.0	36.0	41.0	34.0	41.0
21	37.905647153890385	40.0	36.0	41.0	33.0	41.0
22	37.846342714176934	40.0	36.0	41.0	33.0	41.0
23	37.766017622103774	40.0	35.0	41.0	33.0	41.0
24	37.74443442992339	40.0	35.0	41.0	33.0	41.0
25	37.69043371509378	40.0	35.0	41.0	33.0	41.0
26	37.627822411462134	40.0	35.0	41.0	33.0	41.0
27	37.52505477770353	39.0	35.0	41.0	33.0	41.0
28	37.457111777594754	39.0	35.0	41.0	33.0	41.0
29	37.42950381501453	39.0	35.0	41.0	33.0	41.0
30	37.35606439682367	39.0	35.0	41.0	33.0	41.0
31	37.229332877499964	39.0	35.0	41.0	32.0	41.0
32	37.12884182063993	39.0	35.0	41.0	32.0	41.0
33	36.9980140168762	39.0	35.0	41.0	31.0	41.0
34	36.86335721278613	39.0	35.0	41.0	31.0	41.0
35	36.82488384018897	39.0	35.0	41.0	31.0	41.0
36	36.74023092104241	39.0	35.0	41.0	31.0	41.0
37	36.652536868113934	39.0	35.0	41.0	31.0	41.0
38	36.570623611132696	39.0	35.0	41.0	31.0	41.0
39	36.55599602181784	39.0	35.0	41.0	31.0	41.0
40	36.47042314804743	39.0	35.0	41.0	31.0	41.0
41	36.404974281673944	39.0	35.0	41.0	30.0	41.0
42	36.363924414539014	39.0	35.0	41.0	30.0	41.0
43	36.28830632002611	39.0	35.0	41.0	30.0	41.0
44	36.11695389349039	38.0	35.0	40.0	30.0	41.0
45	35.95963077496853	38.0	35.0	40.0	30.0	41.0
46	35.90576991810539	38.0	35.0	40.0	29.0	41.0
47	35.81835558110985	38.0	35.0	40.0	29.0	41.0
48	35.75270003574148	38.0	35.0	40.0	29.0	41.0
49	35.723162033224035	38.0	35.0	40.0	29.0	41.0
50	35.619000481732996	38.0	35.0	40.0	29.0	41.0
51	34.58520147317058	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	16.0
10	20.0
11	12.0
12	14.0
13	3.0
14	9.0
15	17.0
16	46.0
17	80.0
18	145.0
19	312.0
20	657.0
21	1032.0
22	1600.0
23	2203.0
24	3067.0
25	4161.0
26	5259.0
27	6273.0
28	7254.0
29	8693.0
30	10486.0
31	13342.0
32	17169.0
33	23832.0
34	45427.0
35	59098.0
36	46849.0
37	68278.0
38	121390.0
39	196668.0
40	93.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.32440832310298	20.097589781044583	24.16061910459822	11.417382791254216
2	31.502229957576418	26.134015011421734	24.32938105079952	18.03437398020233
3	31.939363801650323	25.888175785924073	26.280088887507574	15.892371524918028
4	27.75823219530388	25.220276297182636	28.08037171139532	18.941119796118162
5	27.215894080900068	29.63077496853196	24.445152367484578	18.708178583083402
6	26.1751954126587	35.098133673136395	23.88261254681357	14.844058367391339
7	79.70723065686624	5.950801075352365	9.90567357150627	4.436294696275116
8	80.60807135864245	6.762909667293437	7.779210890273655	4.849808083790462
9	75.02556292831503	6.5557644791844725	10.265419340802785	8.15325325169772
10	42.23726127022113	22.281083433046884	19.082220944507466	16.399434352224517
11	34.37770974810026	24.346474802256378	21.94402573386583	19.331789715777532
12	28.935059284238008	22.60462152880297	27.287687837018847	21.172631349940172
13	25.451974328293264	24.937452409441967	30.07319233578344	19.53738092648133
14	22.193283709654864	28.199406380631224	28.402977420708304	21.20433248900561
15	21.284983916333857	24.39915463629159	34.46411089182763	19.851750555546925
16	24.28291712638498	23.9068545943342	29.612127239669935	22.198101039610883
17	23.306553122717595	24.37677736165716	28.594427437025065	23.72224207860018
18	24.85167285667666	23.636617923575393	29.73007412472223	21.781635095025717
19	24.246087861882486	26.278845705583443	27.251946356699975	22.223120075834096
20	26.64667215738683	25.45523768084412	28.13895665957017	19.75913350219888
21	24.000559431865863	28.619912666469833	27.646345822131746	19.73318207953256
22	22.972137185125327	25.18127146431291	27.884415160603567	23.9621761899582
23	22.022656990567356	27.699802644869543	27.202685272956128	23.07485509160697
24	23.412223586269057	24.440801230750107	28.393653556277293	23.753321626703546
25	21.152118848191947	27.970350111109383	26.992432130036832	23.88509891066184
26	22.047986822271604	26.305418719211822	28.53102515889419	23.115569299622383
27	23.19622072695063	27.045422759553077	27.32544948796444	22.432907025531847
28	19.268853630868207	26.72421562990474	30.811020807757455	23.195909931469597
29	23.528771891656692	24.91414274836444	29.13350219887803	22.423583161100836
30	25.03255582663828	25.501390809777625	28.957281161131913	20.508772202452178
31	25.161225155786237	25.394010971080483	26.932137806716288	22.512626066416992
32	25.951111870833397	25.330142499728055	27.057543783313392	21.66120184612516
33	25.563394508243846	24.176780469611973	26.85164177712856	23.408183245015618
34	22.668490000155398	24.0885145529984	30.812419387422107	22.430576059424094
35	23.203369023014407	24.55408618358689	28.879893086354524	23.36265170704418
36	25.15159049587419	26.094854780811488	28.453481686376282	20.300073036938045
37	23.569641497412626	24.78920296498889	30.751192677658466	20.889962859940017
38	22.26212490870383	27.198955727183726	27.830181349163187	22.70873801494926
39	23.693182701123526	23.77196935556557	30.409162250780874	22.12568569253003
40	24.582523970101473	22.585352208978883	28.570806980466507	24.26131684045314
41	21.59919814765893	24.08602818915013	28.651147612313714	25.66362605087722
42	23.178505384531707	23.307640906901213	29.121225777377198	24.39262793118988
43	24.003045795714133	22.495843110441175	29.311121816288793	24.189989277555902
44	22.9764883218598	23.37943466302	29.31718232816895	24.32689468695125
45	22.858230641326475	23.560473030722136	28.4297058320772	25.15159049587419
46	24.41935634255878	24.97241690105826	28.342527699647245	22.265699056735716
47	21.59344843125981	23.91338129943591	31.76733850289817	22.725831766406117
48	22.322263834283852	23.05682895370701	30.49090146229274	24.1300057497164
49	23.460086090348245	21.41753818899473	31.888237945020283	23.234137775636743
50	22.034933412068188	22.7938959767525	29.942813631489802	25.228356979689515
51	21.441003247812777	22.552718683470342	28.22784416714581	27.77843390157107
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	352.0
1	643.0
2	934.0
3	1510.0
4	2086.0
5	1617.5
6	1149.0
7	1181.0
8	1213.0
9	1240.5
10	1268.0
11	1274.0
12	1280.0
13	1345.0
14	1410.0
15	1470.0
16	1530.0
17	1486.5
18	1443.0
19	1430.0
20	1417.0
21	1535.5
22	1654.0
23	1776.0
24	1898.0
25	2238.5
26	3238.0
27	3897.0
28	4692.5
29	5488.0
30	6224.5
31	6961.0
32	8400.0
33	9839.0
34	11702.0
35	13565.0
36	14937.0
37	16309.0
38	18418.0
39	20527.0
40	22803.5
41	25080.0
42	29125.0
43	33170.0
44	38580.5
45	43991.0
46	54536.5
47	65082.0
48	64683.5
49	64285.0
50	66098.0
51	67911.0
52	61516.5
53	55122.0
54	50442.0
55	45762.0
56	40042.0
57	34322.0
58	30956.5
59	27591.0
60	26809.5
61	26028.0
62	23328.0
63	20628.0
64	17200.0
65	13772.0
66	11388.5
67	9005.0
68	7392.0
69	5779.0
70	4833.0
71	3887.0
72	3128.0
73	2369.0
74	1897.0
75	1061.5
76	698.0
77	570.5
78	443.0
79	324.5
80	206.0
81	147.0
82	88.0
83	62.5
84	37.0
85	25.0
86	13.0
87	9.0
88	5.0
89	4.0
90	3.0
91	4.5
92	6.0
93	4.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	643510.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.27058318961756
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.86662969514313	21.328487462387447
2	10.928533794887356	6.397691151675944
3	3.9128471157441265	3.4359395102893076
4	1.8706707149831243	2.190224911331796
5	1.0471321809340361	1.5325084806277685
6	0.7675913895671624	1.3480708574375861
7	0.5684310192781161	1.1646815206137442
8	0.45521855885043183	1.0659610157031505
9	0.3894199581742631	1.0258694353293443
>10	6.751764134376693	48.554828410739
>50	0.3942200015591338	6.9528244786530875
>100	0.04059718173237216	1.9313053872518262
>500	0.0037392141069290145	0.7000043894694321
>1k	0.0032050406630820126	2.3716029884905847
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGC	3610	0.5609858432658389	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCG	3126	0.4857733368556821	No Hit
GCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	2810	0.4366676508523566	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTC	2528	0.39284548802660413	No Hit
CCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	1755	0.2727230346070768	No Hit
CTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGCT	1339	0.208077574552066	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	776	0.12058864664107785	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	744	0.11561591894453856	No Hit
TCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	693	0.10769063417817905	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCT	678	0.10535966807042627	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.1079548103370576E-4	0.0	0.0	0.218644620907212	0.0
2	3.1079548103370576E-4	0.0	0.0	1.1659492470979471	0.0
3	3.1079548103370576E-4	0.0	0.0	1.484048421935945	0.0
4	3.1079548103370576E-4	0.0	0.0	1.9413839722770432	0.0
5	3.1079548103370576E-4	0.0	0.0	3.3997917670277076	0.0
6	3.1079548103370576E-4	0.0	0.0	4.015633012695996	0.0
7	3.1079548103370576E-4	0.0	0.0	4.7660487016518776	0.0
8	3.1079548103370576E-4	0.0	0.0	5.875122375720657	0.0
9	3.1079548103370576E-4	0.0	0.0	6.346132927227238	0.0
10	3.1079548103370576E-4	0.0	0.0	8.017435626485991	0.0
11	3.1079548103370576E-4	0.0	0.0	9.603580363941509	0.0
12	3.1079548103370576E-4	0.0	0.0	11.401221426240463	0.0
13	3.1079548103370576E-4	0.0	0.0	11.93066152818138	0.0
14	3.1079548103370576E-4	0.0	0.0	12.143401034948951	0.0
15	3.1079548103370576E-4	0.0	0.0	12.58535220897888	0.0
16	3.1079548103370576E-4	0.0	0.0	13.356280399682989	0.0
17	3.1079548103370576E-4	0.0	0.0	14.260229056269521	0.0
18	3.1079548103370576E-4	0.0	0.0	15.234262093829155	0.0
19	3.1079548103370576E-4	0.0	0.0	15.926092834610184	0.0
20	4.6619322155055866E-4	0.0	0.0	16.486146291432924	0.0
21	4.6619322155055866E-4	0.0	0.0	17.136641233236467	0.0
22	4.6619322155055866E-4	0.0	0.0	17.806405494864105	0.0
23	4.6619322155055866E-4	0.0	0.0	18.464048732731428	0.0
24	4.6619322155055866E-4	0.0	0.0	18.995975198520615	0.0
25	4.6619322155055866E-4	0.0	0.0	19.476775807679758	0.0
26	4.6619322155055866E-4	0.0	0.0	19.93380056253982	0.0
27	4.6619322155055866E-4	0.0	0.0	20.40620969371105	0.0
28	4.6619322155055866E-4	0.0	0.0	20.85515376606424	0.0
29	4.6619322155055866E-4	0.0	0.0	21.326630510792373	0.0
30	4.6619322155055866E-4	0.0	0.0	21.866637659088436	0.0
31	4.6619322155055866E-4	0.0	0.0	22.341843949588974	0.0
32	4.6619322155055866E-4	0.0	0.0	22.81596245590589	0.0
33	4.6619322155055866E-4	0.0	0.0	23.317586362294293	0.0
34	4.6619322155055866E-4	0.0	0.0	23.777252878743145	0.0
35	4.6619322155055866E-4	0.0	0.0	24.256033317275566	0.0
36	6.215909620674115E-4	0.0	0.0	24.710416310546844	0.0
37	6.215909620674115E-4	0.0	0.0	25.150813507171605	0.0
38	6.215909620674115E-4	0.0	0.0	25.627262979596278	0.0
39	6.215909620674115E-4	0.0	0.0	26.11536728255971	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	20	7.0309703E-4	45.0	1
CGACGGT	20	7.0309703E-4	45.0	28
ACTTGCG	35	1.2108103E-7	45.0	1
AGCCCGA	25	3.8888855E-5	45.0	15
TCGATAG	40	6.8066583E-9	45.0	1
TCTAGCG	20	7.0309703E-4	45.0	1
CCCGTTA	20	7.0309703E-4	45.0	17
CTAACGG	20	7.0309703E-4	45.0	2
TACGCGC	20	7.0309703E-4	45.0	1
CCCCGAT	20	7.0309703E-4	45.0	40
CCGACTC	20	7.0309703E-4	45.0	35
CTCGATC	20	7.0309703E-4	45.0	16
TATAGCG	45	3.8380676E-10	45.0	1
CAATGCG	20	7.0309703E-4	45.0	1
CAGTCGA	20	7.0309703E-4	45.0	12
TTCGCGA	20	7.0309703E-4	45.0	21
AGTACGT	20	7.0309703E-4	45.0	34
AGTCGAA	20	7.0309703E-4	45.0	13
CCCGATC	20	7.0309703E-4	45.0	41
ACGCGTT	20	7.0309703E-4	45.0	16
>>END_MODULE