FastQCFastQC Report
Sat 18 Jun 2016
SRR3550368_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550368_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893259
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTGC14020.1569533584324367No Hit
CTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTGCT10290.11519615251567575No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10200.11418860599221503No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG302.1649557E-645.0000041
AATCCGT207.032442E-445.010
CTCGTAG253.890106E-545.01
TAGAACG253.890106E-545.01
TATTGCG207.032442E-445.01
GGGCGTA207.032442E-445.07
TACGAAT1000.045.012
GCGATAT502.1827873E-1145.09
TCGTAAT207.032442E-445.028
CGGTCTA406.8121153E-945.031
GACGCAC207.032442E-445.09
ATCGCTA207.032442E-445.041
ATCGAGT207.032442E-445.02
GCTACGA1150.041.08695610
TGCGACG451.9274012E-840.01
CACGACC2050.039.51219627
CGAATAT1150.039.13043214
GACGTAG700.038.5714261
TACGTAG659.094947E-1238.076921
AAATGCG659.094947E-1238.076921
Produced by FastQC (version 0.11.5)