FastQCFastQC Report
Sat 18 Jun 2016
SRR3550366_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550366_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences850865
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCTGC15700.18451810804299154No Hit
GCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCTGC14540.17088492299013358No Hit
CTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCTGCT11340.1332761366374219No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC8920.10483449195818371No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8840.10389427229936594No Hit
TCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCTGC8520.10013339366409478No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGTT253.889949E-545.014
TAGCACG253.889949E-545.01
CGGATAC253.889949E-545.014
CGCTAAT207.032252E-445.045
GATATCG207.032252E-445.011
ATGCGAA253.889949E-545.026
TACAGCG253.889949E-545.01
CCGCAAT253.889949E-545.038
TCGAACG302.1648357E-644.9999961
GCGTAAG600.044.9999961
TACGAAT650.041.5384612
CGAATAT650.041.5384614
GCTACGA650.041.5384610
ATGACGG1050.040.7142872
TTGGACG451.9272193E-840.0000041
GCGATTC900.040.0000049
CGTAAGG1250.039.6000022
CGATTCG403.4568075E-739.37510
TGTTGCG800.039.3751
TAGCCGT356.246377E-638.57142644
Produced by FastQC (version 0.11.5)