SRR3550365_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550365_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	871482
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	2684	0.3079811172233047	Illumina Paired End PCR Primer 2 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGCT	1356	0.15559701749433724	TruSeq Adapter, Index 1 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	1316	0.15100713497238039	Illumina Paired End PCR Primer 2 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCG	1162	0.13333608726284651	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTC	1090	0.12507429872332418	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGC	1025	0.11761573962514428	No Hit
TCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	970	0.11130465115745364	Illumina Paired End PCR Primer 2 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	923	0.10591153919415433	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	20	7.032346E-4	45.000004	1
AAGTACG	20	7.032346E-4	45.000004	1
GAATGCG	20	7.032346E-4	45.000004	1
CCGTCCC	20	7.032346E-4	45.000004	20
ACGATAT	20	7.032346E-4	45.000004	27
TACGCAC	20	7.032346E-4	45.000004	30
CTCGCGA	20	7.032346E-4	45.000004	10
CGTACGA	20	7.032346E-4	45.000004	20
TATAGCG	20	7.032346E-4	45.000004	1
CCGGTAT	20	7.032346E-4	45.000004	5
TGCGTCG	20	7.032346E-4	45.000004	2
CTTAACG	25	3.890026E-5	45.0	1
TTAGCCG	25	3.890026E-5	45.0	1
CAATTCG	25	3.890026E-5	45.0	1
CGGTATG	25	3.890026E-5	45.0	6
GTTTACG	30	2.1648957E-6	44.999996	1
ACGTCGG	30	2.1648957E-6	44.999996	2
GCGTACC	30	2.1648957E-6	44.999996	42
ATGCGAG	70	0.0	41.785717	1
CGTATGG	65	0.0	41.53846	2