##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550365_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	871482
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.134957463263724	33.0	31.0	34.0	30.0	34.0
2	32.2890088378188	34.0	31.0	34.0	30.0	34.0
3	32.323413449732755	34.0	31.0	34.0	30.0	34.0
4	35.8319207969872	37.0	35.0	37.0	35.0	37.0
5	35.8344498222568	37.0	35.0	37.0	35.0	37.0
6	35.87573352060054	37.0	35.0	37.0	35.0	37.0
7	36.06799222473901	37.0	35.0	37.0	35.0	37.0
8	36.099251619654794	37.0	35.0	37.0	35.0	37.0
9	37.72789455203894	39.0	38.0	39.0	35.0	39.0
10	37.331523772149055	39.0	37.0	39.0	34.0	39.0
11	37.21644738502918	39.0	37.0	39.0	34.0	39.0
12	37.069138547898866	39.0	37.0	39.0	34.0	39.0
13	36.98308169302407	39.0	37.0	39.0	33.0	39.0
14	38.147733401263594	40.0	37.0	41.0	33.0	41.0
15	38.26924709862051	40.0	37.0	41.0	34.0	41.0
16	38.34026864582401	40.0	37.0	41.0	34.0	41.0
17	38.32298314824632	40.0	37.0	41.0	34.0	41.0
18	38.28838117138392	40.0	37.0	41.0	34.0	41.0
19	38.22003667316135	40.0	37.0	41.0	34.0	41.0
20	38.16306131394567	40.0	37.0	41.0	34.0	41.0
21	38.11480787899234	40.0	37.0	41.0	34.0	41.0
22	38.07408299884565	40.0	36.0	41.0	34.0	41.0
23	37.993164517454176	40.0	36.0	41.0	34.0	41.0
24	37.94764321007204	40.0	36.0	41.0	34.0	41.0
25	37.89996810031647	40.0	36.0	41.0	33.0	41.0
26	37.83448309890508	40.0	36.0	41.0	33.0	41.0
27	37.77572457032962	40.0	36.0	41.0	33.0	41.0
28	37.74669126843698	40.0	36.0	41.0	33.0	41.0
29	37.70539953779883	40.0	36.0	41.0	33.0	41.0
30	37.67497091161952	40.0	36.0	41.0	33.0	41.0
31	37.60192293128257	40.0	36.0	41.0	33.0	41.0
32	37.51940946571472	40.0	36.0	41.0	33.0	41.0
33	37.38533899724837	40.0	35.0	41.0	33.0	41.0
34	37.3462825393984	40.0	35.0	41.0	33.0	41.0
35	37.28981321473077	40.0	35.0	41.0	32.0	41.0
36	37.244317151702504	40.0	35.0	41.0	32.0	41.0
37	37.13419324782382	39.0	35.0	41.0	32.0	41.0
38	37.10950656467948	39.0	35.0	41.0	32.0	41.0
39	37.02592136154275	39.0	35.0	41.0	32.0	41.0
40	36.92131908633799	39.0	35.0	41.0	31.0	41.0
41	36.860166934027326	39.0	35.0	41.0	31.0	41.0
42	36.85850195414248	39.0	35.0	41.0	31.0	41.0
43	36.82184256243961	39.0	35.0	41.0	31.0	41.0
44	36.69141187081317	39.0	35.0	41.0	31.0	41.0
45	36.55422372464377	39.0	35.0	41.0	31.0	41.0
46	36.48894412047524	39.0	35.0	41.0	31.0	41.0
47	36.405673324291264	39.0	35.0	40.0	31.0	41.0
48	36.36206140803826	38.0	35.0	40.0	31.0	41.0
49	36.33153754179662	38.0	35.0	40.0	31.0	41.0
50	36.23900206774208	38.0	35.0	40.0	31.0	41.0
51	35.25162080226556	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	10.0
10	19.0
11	16.0
12	8.0
13	14.0
14	16.0
15	21.0
16	57.0
17	90.0
18	171.0
19	291.0
20	533.0
21	958.0
22	1419.0
23	2094.0
24	3168.0
25	4329.0
26	5679.0
27	6990.0
28	8097.0
29	10164.0
30	12804.0
31	16268.0
32	21312.0
33	29964.0
34	54348.0
35	75000.0
36	63753.0
37	93875.0
38	166558.0
39	293320.0
40	127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.40936703225081	21.860692475576087	25.792730085073472	9.93721040709963
2	31.510461489738166	25.74924094817793	25.72698001794644	17.013317544137458
3	31.64058465923565	24.888178987058826	26.661824340606	16.809412013099525
4	27.916812969172057	26.63657998673524	26.644382787022565	18.80222425707014
5	25.9548676851616	31.37242077289032	24.176861943218565	18.49584959872952
6	26.45585336243319	35.69884403808685	23.632042887862283	14.213259711617681
7	81.68579500207692	6.122100054849096	7.8750909370474655	4.317014006026516
8	83.23763428275053	5.404242428415045	6.885856506502716	4.472266782331706
9	77.29316268150117	6.891479112592114	9.3075932721502	6.50776493375652
10	41.887726883630414	22.69651008282443	19.211182789776498	16.20458024376866
11	32.337558320194795	24.96345306041892	22.946658680271078	19.75232993911521
12	28.315903254456202	23.642714364725833	27.953991017599904	20.087391363218057
13	24.66109454928501	25.976784374203945	29.968031468234567	19.394089608276477
14	21.77658287836123	28.973518672789574	28.992222444066545	20.257676004782656
15	20.596179840776976	25.30287487291763	34.365827406647526	19.73511787965787
16	23.05796333142853	24.423682875836793	30.816471252418292	21.70188254031638
17	22.889858884061862	24.607507670841162	28.09065476969117	24.411978675405805
18	23.84122678380047	24.44732077082487	30.01461877583243	21.69683366954223
19	24.25488994609183	26.5661252900232	27.27032801595443	21.908656747930536
20	25.256402312382814	26.577026261012847	27.190693554198482	20.975877872405857
21	23.42423595668069	28.039018591319152	27.938959152340498	20.59778629965966
22	22.766391044221223	25.139360308072916	26.65872616990368	25.43552247780218
23	21.877101305592085	27.087421197454454	27.000557670726415	24.034919826227046
24	22.04233707638253	26.21958915961546	28.657046272900644	23.081027491101366
25	21.412146206117853	27.360174966321736	27.563506762044426	23.664172065515984
26	20.406961933809303	27.924730516522427	29.11477230740279	22.553535242265475
27	22.047959682471927	26.757179149999654	28.701223892174482	22.493637275353937
28	19.641140035020804	27.277327586800414	29.51696076338926	23.56457161478952
29	21.352362986269366	24.939470924241693	29.337840597969894	24.370325491519047
30	23.394631214414066	26.279831367716145	27.388058502642625	22.937478915227164
31	23.317635935108243	27.260115527343075	25.295760555008595	24.126487982540088
32	23.038571077773266	26.43324819101255	26.668020682010646	23.86016004920354
33	23.794983717391755	26.095432837396526	26.513226893957647	23.596356551254072
34	21.54422007568716	25.95245799683757	27.636485894143544	24.866836033331726
35	22.225588136071657	24.54772445099268	28.44281350618831	24.783873906747356
36	23.800032588165905	25.91332924833789	28.35985137960394	21.926786783892265
37	21.806646608880044	26.498424522824337	28.196681055948375	23.49824781234724
38	21.630280372973854	26.662053834732102	26.779899068483342	24.927766723810706
39	22.748031514133395	25.666737809845756	28.21274564477522	23.372485031245624
40	22.846943482481567	25.507239392207758	29.101805889278264	22.544011236032414
41	20.103685446171006	25.60408591342105	29.438818013452945	24.853410626955004
42	21.297858131321128	24.22413773319472	30.661218476113106	23.81678565937105
43	22.709935489201154	23.974562871063316	29.097560247945452	24.217941391790077
44	21.59551201287003	24.632866771774978	27.389091226210066	26.382529989144928
45	22.06482750074012	25.544302693572558	26.80434019291276	25.586529612774562
46	23.411269538556162	25.81946615076387	27.745151362850866	23.0241129478291
47	20.760727129189128	25.586300118648463	30.375383542058245	23.277589210104168
48	21.355805398160836	23.67622050713612	29.76171624887261	25.206257845830436
49	21.931032425225077	22.919348879265435	30.75508157368712	24.394537121822367
50	21.363034463132916	23.46026653447805	29.88793801822642	25.28876098416261
51	21.433489159844953	23.864176196410252	27.29167096968153	27.41066367406326
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	303.0
1	704.5
2	1106.0
3	1746.5
4	2387.0
5	1824.5
6	1262.0
7	1302.5
8	1343.0
9	1368.5
10	1394.0
11	1492.5
12	1591.0
13	1571.0
14	1551.0
15	1534.5
16	1518.0
17	1513.0
18	1508.0
19	1506.5
20	1505.0
21	1761.5
22	2018.0
23	2528.0
24	3038.0
25	3044.5
26	4514.5
27	5978.0
28	7451.5
29	8925.0
30	11349.5
31	13774.0
32	15287.5
33	16801.0
34	18837.5
35	20874.0
36	21643.5
37	22413.0
38	26349.0
39	30285.0
40	35798.0
41	41311.0
42	46156.0
43	51001.0
44	56916.0
45	62831.0
46	72500.5
47	82170.0
48	88887.0
49	95604.0
50	91981.0
51	88358.0
52	78519.0
53	68680.0
54	61063.5
55	53447.0
56	48491.0
57	43535.0
58	38640.0
59	33745.0
60	32140.5
61	30536.0
62	26775.0
63	23014.0
64	20190.0
65	17366.0
66	15181.5
67	12997.0
68	10653.0
69	8309.0
70	7018.5
71	5728.0
72	4923.5
73	4119.0
74	3600.0
75	2343.5
76	1606.0
77	1222.5
78	839.0
79	561.5
80	284.0
81	233.0
82	182.0
83	124.5
84	67.0
85	47.5
86	28.0
87	18.0
88	8.0
89	9.0
90	10.0
91	5.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	871482.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.724570491994278
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.86135307566465	19.646005785043602
2	11.805844671505051	6.546239456253536
3	4.4799648623423725	3.726153048830057
4	2.236137733153767	2.479838328505196
5	1.3578731579206904	1.8823225042979528
6	0.8919862082555994	1.4837960705201432
7	0.6653701501895773	1.291297114253983
8	0.5005684455877765	1.1102436124613049
9	0.426369623905548	1.063882322425301
>10	5.740949191505311	39.59106617464319
>50	0.9647607755940611	16.55909835070035
>100	0.06339975675810439	3.0635401529805
>500	0.00291972564017586	0.5581952407684159
>1k	0.002502621977293594	0.9983218383164978
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	2684	0.3079811172233047	Illumina Paired End PCR Primer 2 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGCT	1356	0.15559701749433724	TruSeq Adapter, Index 1 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	1316	0.15100713497238039	Illumina Paired End PCR Primer 2 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCG	1162	0.13333608726284651	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTC	1090	0.12507429872332418	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGC	1025	0.11761573962514428	No Hit
TCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	970	0.11130465115745364	Illumina Paired End PCR Primer 2 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	923	0.10591153919415433	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2949412609784252E-4	0.0	0.0	0.16041639414239192	0.0
2	2.2949412609784252E-4	0.0	0.0	0.840866478022495	0.0
3	2.2949412609784252E-4	0.0	0.0	1.155617671965686	0.0
4	2.2949412609784252E-4	0.0	0.0	1.5705430519505854	0.0
5	2.2949412609784252E-4	0.0	0.0	2.8659226467098575	0.0
6	2.2949412609784252E-4	0.0	0.0	3.615335715482362	0.0
7	2.2949412609784252E-4	0.0	0.0	4.3843705320362325	0.0
8	2.2949412609784252E-4	0.0	0.0	5.744582217418145	0.0
9	2.2949412609784252E-4	0.0	0.0	6.275172636956357	0.0
10	2.2949412609784252E-4	0.0	0.0	7.617713274628736	0.0
11	2.2949412609784252E-4	0.0	0.0	9.164388937465146	0.0
12	2.2949412609784252E-4	0.0	0.0	10.47548887986212	0.0
13	2.2949412609784252E-4	0.0	0.0	10.922428690437668	0.0
14	2.2949412609784252E-4	0.0	0.0	11.096499985082882	0.0
15	2.2949412609784252E-4	0.0	0.0	11.470919651811512	0.0
16	2.2949412609784252E-4	0.0	0.0	12.289525199602517	0.0
17	2.2949412609784252E-4	0.0	0.0	13.274284494688358	0.0
18	2.2949412609784252E-4	0.0	0.0	14.387216259199846	0.0
19	2.2949412609784252E-4	0.0	0.0	15.005243940781336	0.0
20	2.2949412609784252E-4	0.0	0.0	15.591716180024372	0.0
21	2.2949412609784252E-4	0.0	0.0	16.38542161513376	0.0
22	2.2949412609784252E-4	0.0	0.0	17.11968807158381	0.0
23	2.2949412609784252E-4	0.0	0.0	17.944719454905552	0.0
24	2.2949412609784252E-4	0.0	0.0	18.596482773023425	0.0
25	2.2949412609784252E-4	0.0	0.0	19.172742523655106	0.0
26	2.2949412609784252E-4	0.0	0.0	19.720774496776755	0.0
27	2.2949412609784252E-4	0.0	0.0	20.2319726626597	0.0
28	2.2949412609784252E-4	0.0	0.0	20.76038288799998	0.0
29	2.2949412609784252E-4	0.0	0.0	21.300038325519058	0.0
30	3.442411891467638E-4	0.0	0.0	21.943195613908262	0.0
31	3.442411891467638E-4	0.0	0.0	22.5348314709885	0.0
32	3.442411891467638E-4	0.0	0.0	23.075519632075018	0.0
33	3.442411891467638E-4	0.0	0.0	23.625961293520692	0.0
34	3.442411891467638E-4	0.0	0.0	24.150125877528165	0.0
35	3.442411891467638E-4	0.0	0.0	24.7152551630441	0.0
36	3.442411891467638E-4	0.0	0.0	25.273270130651007	0.0
37	3.442411891467638E-4	0.0	0.0	25.803401561937022	0.0
38	3.442411891467638E-4	0.0	0.0	26.357515129400262	0.0
39	3.442411891467638E-4	0.0	0.0	26.889138272505914	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	20	7.032346E-4	45.000004	1
AAGTACG	20	7.032346E-4	45.000004	1
GAATGCG	20	7.032346E-4	45.000004	1
CCGTCCC	20	7.032346E-4	45.000004	20
ACGATAT	20	7.032346E-4	45.000004	27
TACGCAC	20	7.032346E-4	45.000004	30
CTCGCGA	20	7.032346E-4	45.000004	10
CGTACGA	20	7.032346E-4	45.000004	20
TATAGCG	20	7.032346E-4	45.000004	1
CCGGTAT	20	7.032346E-4	45.000004	5
TGCGTCG	20	7.032346E-4	45.000004	2
CTTAACG	25	3.890026E-5	45.0	1
TTAGCCG	25	3.890026E-5	45.0	1
CAATTCG	25	3.890026E-5	45.0	1
CGGTATG	25	3.890026E-5	45.0	6
GTTTACG	30	2.1648957E-6	44.999996	1
ACGTCGG	30	2.1648957E-6	44.999996	2
GCGTACC	30	2.1648957E-6	44.999996	42
ATGCGAG	70	0.0	41.785717	1
CGTATGG	65	0.0	41.53846	2
>>END_MODULE