FastQCFastQC Report
Sat 18 Jun 2016
SRR3550363_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550363_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203981
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC13440.6588848961422877No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11010.5397561537594188No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC10690.5240684181369833No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT8760.42945176266416973Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCC7360.3608179193160147No Hit
TCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC5890.288752383800452No Hit
ACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC3770.18482113530181732No Hit
CGTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTC2750.1348164780053044No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTC2420.11863850064466788No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCT2340.11471656673905904No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCG2160.10589221545143909No Hit
AGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG2060.10098979806942804No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGC2040.10000931459302582No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAGG302.1566957E-645.0000042
TCGTGCG302.1566957E-645.0000041
CGTGGCA207.01963E-445.015
CCTTATA207.01963E-445.021
AACCGGG207.01963E-445.03
ACACGTG207.01963E-445.042
CGTGCGG406.7684596E-945.02
GACACGA207.01963E-445.025
CGGCATC207.01963E-445.022
CGATTAA207.01963E-445.032
CGCGAGG207.01963E-445.02
GAATAAG207.01963E-445.01
TCGGCAT207.01963E-445.021
GTTACGG207.01963E-445.02
CCCAATC207.01963E-445.040
TGCGCGC207.01963E-445.015
GAGCGTC207.01963E-445.016
CTACGGG253.879491E-545.03
TACGAGG207.01963E-445.02
ACGTGGG253.879491E-545.03