##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550363_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	203981
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09697471823356	33.0	31.0	34.0	30.0	34.0
2	32.23465420798996	34.0	31.0	34.0	30.0	34.0
3	32.18972355268383	34.0	31.0	34.0	30.0	34.0
4	35.76552718145317	37.0	35.0	37.0	35.0	37.0
5	35.884959873713726	37.0	35.0	37.0	35.0	37.0
6	35.950510096528596	37.0	35.0	37.0	35.0	37.0
7	36.072393997480155	37.0	35.0	37.0	35.0	37.0
8	35.999004809271455	37.0	35.0	37.0	35.0	37.0
9	37.84920164132934	39.0	38.0	39.0	35.0	39.0
10	37.50411067697482	39.0	37.0	39.0	35.0	39.0
11	37.45455704207745	39.0	37.0	39.0	35.0	39.0
12	37.55451733249665	39.0	37.0	39.0	35.0	39.0
13	37.61096376623313	39.0	37.0	39.0	35.0	39.0
14	38.8281751731779	40.0	38.0	41.0	35.0	41.0
15	38.884871630200855	40.0	38.0	41.0	35.0	41.0
16	38.92713537045117	40.0	38.0	41.0	35.0	41.0
17	38.86379613787559	40.0	38.0	41.0	35.0	41.0
18	38.811153980027555	40.0	38.0	41.0	35.0	41.0
19	38.776719400336304	40.0	38.0	41.0	35.0	41.0
20	38.694074448110364	40.0	38.0	41.0	35.0	41.0
21	38.63049499708306	40.0	38.0	41.0	35.0	41.0
22	38.60145307651203	40.0	38.0	41.0	35.0	41.0
23	38.555904716615764	40.0	38.0	41.0	35.0	41.0
24	38.51392041415622	40.0	38.0	41.0	34.0	41.0
25	38.444105088219004	40.0	38.0	41.0	34.0	41.0
26	38.5114741078826	40.0	38.0	41.0	35.0	41.0
27	38.51217515356822	40.0	38.0	41.0	35.0	41.0
28	38.47603943504542	40.0	38.0	41.0	35.0	41.0
29	38.42836832842274	40.0	38.0	41.0	34.0	41.0
30	38.352841686235486	40.0	38.0	41.0	34.0	41.0
31	38.318407106544235	40.0	38.0	41.0	34.0	41.0
32	38.21719669969262	40.0	38.0	41.0	34.0	41.0
33	38.14042974590771	40.0	38.0	41.0	34.0	41.0
34	38.04254317804109	40.0	38.0	41.0	34.0	41.0
35	37.96789897098259	40.0	38.0	41.0	34.0	41.0
36	37.87167922502586	40.0	37.0	41.0	34.0	41.0
37	37.82763590726587	40.0	37.0	41.0	34.0	41.0
38	37.78728410979454	40.0	37.0	41.0	33.0	41.0
39	37.7166010559807	40.0	37.0	41.0	33.0	41.0
40	37.63983410219579	40.0	37.0	41.0	33.0	41.0
41	37.57990695211809	40.0	37.0	41.0	33.0	41.0
42	37.503522386888974	40.0	37.0	41.0	33.0	41.0
43	37.47581392384585	40.0	37.0	41.0	33.0	41.0
44	37.4406979081385	40.0	37.0	41.0	33.0	41.0
45	37.38808026237738	40.0	37.0	41.0	33.0	41.0
46	37.29881704668572	40.0	36.0	41.0	32.0	41.0
47	37.2034405165187	40.0	36.0	41.0	32.0	41.0
48	37.20022943313348	40.0	36.0	41.0	32.0	41.0
49	37.19407199690167	40.0	36.0	41.0	32.0	41.0
50	37.13173285747202	40.0	36.0	41.0	32.0	41.0
51	36.18893916590271	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	4.0
12	0.0
13	0.0
14	4.0
15	6.0
16	9.0
17	14.0
18	17.0
19	29.0
20	64.0
21	120.0
22	165.0
23	257.0
24	501.0
25	882.0
26	1244.0
27	1518.0
28	1805.0
29	2024.0
30	2419.0
31	2977.0
32	3783.0
33	5258.0
34	8609.0
35	11625.0
36	12938.0
37	19987.0
38	40585.0
39	87045.0
40	84.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.15373980909987	21.550536569582462	25.095474578514665	13.200249042803005
2	33.75902657600463	23.17225623955172	25.75435947465695	17.314357709786695
3	31.105348047122032	23.480128051142017	29.525789166638067	15.888734735097875
4	28.58746647972115	25.190581475725683	26.956432216726068	19.265519827827102
5	23.96644785543752	29.22232953069158	26.413734612537443	20.397488001333457
6	24.639059520249436	33.471744917418775	26.452953951593532	15.436241610738255
7	77.73125928395291	4.971051225359225	11.551566077232684	5.746123413455175
8	76.17032958952059	4.2087253224565035	12.267319015006299	7.3536260730166045
9	70.12025629838074	7.5845299317093255	14.531745603757212	7.76346816615273
10	30.845519925875447	34.471347821610834	21.428466376770388	13.25466587574333
11	19.93420955873341	22.859482010579416	34.420852922576124	22.78545550811105
12	19.559664870747763	21.032841294042093	38.67517072668532	20.732323108524813
13	20.746049877194444	22.35649398718508	38.531039655654205	18.366416479966272
14	17.271216436825	27.46775434966982	34.27329015937759	20.98773905412759
15	15.44800741245508	26.355886087429713	37.93686666895446	20.259239831160745
16	17.72812173682843	27.75993842563768	33.754614400360815	20.75732543717307
17	16.786367357744105	27.438339845377758	31.241635250341943	24.533657546536197
18	17.043254028561485	27.663851044950267	34.8963874086312	20.396507517857053
19	17.76292890024071	27.898676837548596	31.671577254744314	22.66681700746638
20	19.311112309479807	29.567459714385162	31.693638132963365	19.427789843171666
21	19.035596452610783	27.380981562008227	33.71392433609013	19.869497649290864
22	16.66037523102642	25.736220530343513	31.263205886822792	26.340198351807274
23	17.016290732960424	26.393144459532998	32.26231854927665	24.32824625822993
24	20.228844843392277	25.12341835759213	31.87453733435957	22.77319946465602
25	17.00158348081439	28.461474353003467	29.296356033159952	25.24058613302219
26	16.663316681455626	28.851706776611547	30.75727641299925	23.727700128933577
27	20.586721312279085	25.703864575622237	32.361347380393276	21.348066731705405
28	17.150126727489326	26.672092008569425	30.76021786342846	25.417563400512794
29	17.59967840141974	26.64316774601556	30.16359366803771	25.593560184526993
30	20.129816012275654	25.044489437741753	31.681382089508336	23.14431246047426
31	21.328457062177357	26.967217534966494	28.297733612444297	23.406591790411852
32	19.39249243802119	27.87661595932955	26.668660316402015	26.06223128624725
33	19.02677210132316	23.1614709213113	30.72001804089596	27.09173893646957
34	18.863031360763994	23.070285958005893	32.255945406680034	25.810737274550082
35	19.60672807761507	23.979684382368944	30.530784729950337	25.88280281006564
36	19.577313573323003	24.249807580117754	33.92031610787279	22.252562738686446
37	20.551423907128605	22.808987111544702	34.619891068285774	22.01969791304092
38	20.779876557130322	23.49385481981165	28.808565503649852	26.91770311940818
39	20.42984395605473	20.74948156936185	31.645104200881452	27.17557027370196
40	20.497497315926484	21.273059745760634	34.188968580407	24.040474357905882
41	19.895970703153726	24.715537231408806	31.424005176952758	23.96448688848471
42	21.8338962942627	23.053617738907057	34.18014422911938	20.932341737710868
43	22.43101073139165	21.811345174305448	32.6388242042151	23.118819890087803
44	19.820963717208954	21.85056451336154	29.525298924899868	28.80317284452964
45	21.369637368186254	19.695461832229473	29.34734117393287	29.587559625651412
46	23.381589461763596	20.994602438462408	31.0896603114996	24.5341477882744
47	18.929704237159342	21.902039895872655	34.737058843715836	24.431197023252167
48	19.401316789308808	20.60731146528353	33.13740005196563	26.853971693442034
49	21.597599776449766	18.362494546060663	35.55380157955888	24.48610409793069
50	19.395924130188597	17.477608208607663	34.06591790411852	29.06054975708522
51	18.531137704001843	17.256999426417167	30.87836612233492	33.333496747246066
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	157.0
1	183.5
2	210.0
3	956.5
4	1703.0
5	1143.0
6	583.0
7	596.5
8	610.0
9	593.5
10	577.0
11	549.5
12	522.0
13	557.5
14	593.0
15	558.5
16	524.0
17	529.0
18	534.0
19	637.5
20	741.0
21	749.0
22	757.0
23	870.0
24	983.0
25	1119.0
26	1683.5
27	2112.0
28	2261.5
29	2411.0
30	2915.0
31	3419.0
32	3774.5
33	4130.0
34	4607.0
35	5084.0
36	5293.5
37	5503.0
38	6423.0
39	7343.0
40	8266.5
41	9190.0
42	10475.0
43	11760.0
44	13493.5
45	15227.0
46	19133.0
47	23039.0
48	27100.0
49	31161.0
50	29354.0
51	27547.0
52	21671.0
53	15795.0
54	12786.5
55	9778.0
56	8032.5
57	6287.0
58	5468.0
59	4649.0
60	4037.5
61	3426.0
62	2955.5
63	2485.0
64	2014.0
65	1543.0
66	1168.5
67	794.0
68	717.0
69	640.0
70	481.0
71	322.0
72	293.5
73	265.0
74	218.5
75	118.5
76	65.0
77	52.5
78	40.0
79	31.0
80	22.0
81	18.5
82	15.0
83	9.0
84	3.0
85	3.5
86	4.0
87	2.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	203981.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.258627028987995
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.00055243232704	32.13387521386796
2	12.711359525124838	11.505973595579981
3	4.916647710655445	6.675621749084473
4	2.689587192235618	4.869080943813394
5	1.7764490516578384	4.019982253249077
6	1.322587982972086	3.59151097406131
7	0.9618821694342442	3.0473426446580807
8	0.8026516751697916	2.906153024056162
9	0.6564195886003965	2.6737784401488374
>10	3.0459602032084403	20.702418362494544
>50	0.06499203847528678	1.9364548658943725
>100	0.04441122629144596	3.1360763992724814
>500	0.003249601923764339	1.0790220657806364
>1k	0.003249601923764339	1.7227094680386899
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	1344	0.6588848961422877	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1101	0.5397561537594188	No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	1069	0.5240684181369833	No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT	876	0.42945176266416973	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCC	736	0.3608179193160147	No Hit
TCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	589	0.288752383800452	No Hit
ACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	377	0.18482113530181732	No Hit
CGTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTC	275	0.1348164780053044	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTC	242	0.11863850064466788	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCT	234	0.11471656673905904	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCG	216	0.10589221545143909	No Hit
AGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG	206	0.10098979806942804	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGC	204	0.10000931459302582	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4382761139517896	0.0
2	4.90241738201107E-4	0.0	0.0	2.1472588133208483	0.0
3	4.90241738201107E-4	0.0	0.0	2.855658125021448	0.0
4	4.90241738201107E-4	0.0	0.0	4.0969502061466505	0.0
5	4.90241738201107E-4	0.0	0.0	7.837004426882896	0.0
6	4.90241738201107E-4	0.0	0.0	9.617072178291115	0.0
7	4.90241738201107E-4	0.0	0.0	11.639319348370682	0.0
8	4.90241738201107E-4	0.0	0.0	14.518509076825783	0.0
9	4.90241738201107E-4	0.0	0.0	15.579392198292979	0.0
10	4.90241738201107E-4	0.0	0.0	17.818326216657432	0.0
11	4.90241738201107E-4	0.0	0.0	21.463763781920864	0.0
12	4.90241738201107E-4	0.0	0.0	23.9595844711027	0.0
13	4.90241738201107E-4	0.0	0.0	24.926341178835283	0.0
14	4.90241738201107E-4	0.0	0.0	25.280785955554684	0.0
15	4.90241738201107E-4	0.0	0.0	25.986243816826075	0.0
16	4.90241738201107E-4	0.0	0.0	27.40451316544188	0.0
17	4.90241738201107E-4	0.0	0.0	29.42038719292483	0.0
18	4.90241738201107E-4	0.0	0.0	31.27546193027782	0.0
19	4.90241738201107E-4	0.0	0.0	32.52901005485805	0.0
20	4.90241738201107E-4	0.0	0.0	33.65362460229139	0.0
21	4.90241738201107E-4	0.0	0.0	35.02532098577809	0.0
22	4.90241738201107E-4	0.0	0.0	36.543109407248714	0.0
23	4.90241738201107E-4	0.0	0.0	37.92804231766684	0.0
24	4.90241738201107E-4	0.0	0.0	38.93794029836112	0.0
25	4.90241738201107E-4	0.0	0.0	39.84096558012756	0.0
26	4.90241738201107E-4	0.0	0.0	40.67780822723685	0.0
27	4.90241738201107E-4	0.0	0.0	41.53769223604159	0.0
28	4.90241738201107E-4	0.0	0.0	42.446110176928244	0.0
29	4.90241738201107E-4	0.0	0.0	43.31775998744981	0.0
30	4.90241738201107E-4	0.0	0.0	44.246277839602705	0.0
31	4.90241738201107E-4	0.0	0.0	45.0826302449738	0.0
32	4.90241738201107E-4	0.0	0.0	45.88956814605282	0.0
33	4.90241738201107E-4	0.0	0.0	46.708762090586866	0.0
34	4.90241738201107E-4	0.0	0.0	47.436771071815514	0.0
35	4.90241738201107E-4	0.0	0.0	48.21429446860247	0.0
36	4.90241738201107E-4	0.0	0.0	48.97760085498159	0.0
37	4.90241738201107E-4	0.0	0.0	49.696785484922614	0.0
38	4.90241738201107E-4	0.0	0.0	50.43361881743888	0.0
39	4.90241738201107E-4	0.0	0.0	51.131723052637255	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACAGG	30	2.1566957E-6	45.000004	2
TCGTGCG	30	2.1566957E-6	45.000004	1
CGTGGCA	20	7.01963E-4	45.0	15
CCTTATA	20	7.01963E-4	45.0	21
AACCGGG	20	7.01963E-4	45.0	3
ACACGTG	20	7.01963E-4	45.0	42
CGTGCGG	40	6.7684596E-9	45.0	2
GACACGA	20	7.01963E-4	45.0	25
CGGCATC	20	7.01963E-4	45.0	22
CGATTAA	20	7.01963E-4	45.0	32
CGCGAGG	20	7.01963E-4	45.0	2
GAATAAG	20	7.01963E-4	45.0	1
TCGGCAT	20	7.01963E-4	45.0	21
GTTACGG	20	7.01963E-4	45.0	2
CCCAATC	20	7.01963E-4	45.0	40
TGCGCGC	20	7.01963E-4	45.0	15
GAGCGTC	20	7.01963E-4	45.0	16
CTACGGG	25	3.879491E-5	45.0	3
TACGAGG	20	7.01963E-4	45.0	2
ACGTGGG	25	3.879491E-5	45.0	3
>>END_MODULE