SRR3550362_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550362_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	347743
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	1083	0.31143689448817086	Illumina PCR Primer Index 7 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	973	0.2798043382613022	RNA PCR Primer, Index 16 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	731	0.21021271456219104	No Hit
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	711	0.20446134070276037	RNA PCR Primer, Index 16 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	374	0.10755069117135356	Illumina PCR Primer Index 7 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCC	351	0.10093661123300827	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCACGG	30	2.1611177E-6	45.000004	2
TGCGATG	25	3.8851744E-5	45.000004	1
CACGACG	35	1.2086639E-7	45.0	26
ACGGGTA	35	1.2086639E-7	45.0	5
TCGATAG	20	7.0264924E-4	45.0	1
TATTGCG	20	7.0264924E-4	45.0	1
GCCGACC	20	7.0264924E-4	45.0	14
TATTAGG	20	7.0264924E-4	45.0	2
GTTCACG	20	7.0264924E-4	45.0	1
CGGCTAT	20	7.0264924E-4	45.0	35
CGGTAGT	40	6.7921064E-9	45.0	12
ATACAGG	35	1.2086639E-7	45.0	2
AATCGCG	20	7.0264924E-4	45.0	1
TAGTACG	20	7.0264924E-4	45.0	1
ATTTCCG	55	1.8189894E-12	45.0	32
AGACACG	90	0.0	42.5	24
ACACGAC	80	0.0	42.1875	26
CGACCCG	60	3.6379788E-12	41.250004	32
CATGCAT	90	0.0	40.0	25
CGCCGGT	45	1.9215804E-8	40.0	28