##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3550362_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 347743 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.41968062620958 33.0 31.0 34.0 31.0 34.0 2 32.54955815070325 34.0 31.0 34.0 31.0 34.0 3 32.534926655604856 34.0 31.0 34.0 31.0 34.0 4 36.01783788602503 37.0 35.0 37.0 35.0 37.0 5 36.093275781252245 37.0 35.0 37.0 35.0 37.0 6 36.13040952657566 37.0 36.0 37.0 35.0 37.0 7 36.157918347745316 37.0 37.0 37.0 35.0 37.0 8 36.085301501396145 37.0 37.0 37.0 35.0 37.0 9 37.927544192118894 39.0 38.0 39.0 35.0 39.0 10 37.66468052556054 39.0 37.0 39.0 35.0 39.0 11 37.59409966555761 39.0 37.0 39.0 35.0 39.0 12 37.49969661502892 39.0 37.0 39.0 35.0 39.0 13 37.47126469835482 39.0 37.0 39.0 35.0 39.0 14 38.76088663179417 40.0 38.0 41.0 35.0 41.0 15 38.845368562415345 40.0 38.0 41.0 35.0 41.0 16 38.882059451951584 40.0 38.0 41.0 35.0 41.0 17 38.83815633959562 40.0 38.0 41.0 35.0 41.0 18 38.80303269943608 40.0 38.0 41.0 35.0 41.0 19 38.75313665551859 40.0 38.0 41.0 35.0 41.0 20 38.70518170027865 40.0 38.0 41.0 35.0 41.0 21 38.63825296267646 40.0 38.0 41.0 35.0 41.0 22 38.61639774201062 40.0 38.0 41.0 35.0 41.0 23 38.57902819035897 40.0 38.0 41.0 35.0 41.0 24 38.53389428399709 40.0 38.0 41.0 35.0 41.0 25 38.45335779584348 40.0 37.0 41.0 34.0 41.0 26 38.45457708710168 40.0 37.0 41.0 35.0 41.0 27 38.443149107242995 40.0 37.0 41.0 35.0 41.0 28 38.366583942739325 40.0 37.0 41.0 34.0 41.0 29 38.33112672289593 40.0 37.0 41.0 34.0 41.0 30 38.24014861550053 40.0 37.0 41.0 34.0 41.0 31 38.163928533428425 40.0 37.0 41.0 34.0 41.0 32 38.07240116982944 40.0 37.0 41.0 34.0 41.0 33 38.00173116353169 40.0 37.0 41.0 34.0 41.0 34 37.90028843139905 40.0 37.0 41.0 34.0 41.0 35 37.84943190804704 40.0 36.0 41.0 34.0 41.0 36 37.76895580931895 40.0 36.0 41.0 33.0 41.0 37 37.692761608429215 40.0 36.0 41.0 33.0 41.0 38 37.63505807449754 40.0 36.0 41.0 33.0 41.0 39 37.57291160425947 40.0 36.0 41.0 33.0 41.0 40 37.443103096252116 40.0 35.0 41.0 33.0 41.0 41 37.395018735100344 40.0 35.0 41.0 33.0 41.0 42 37.33937419301036 40.0 35.0 41.0 33.0 41.0 43 37.283700894051066 40.0 35.0 41.0 33.0 41.0 44 37.195794020296596 39.0 35.0 41.0 33.0 41.0 45 37.070060360668656 39.0 35.0 41.0 33.0 41.0 46 36.97894709598756 39.0 35.0 41.0 32.0 41.0 47 36.89725170600127 39.0 35.0 41.0 32.0 41.0 48 36.85252039580955 39.0 35.0 41.0 32.0 41.0 49 36.79816991283793 39.0 35.0 41.0 32.0 41.0 50 36.717696689796774 39.0 35.0 41.0 32.0 41.0 51 35.80453668370032 38.0 35.0 40.0 30.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 2.0 9 11.0 10 4.0 11 5.0 12 5.0 13 8.0 14 5.0 15 12.0 16 13.0 17 30.0 18 46.0 19 77.0 20 168.0 21 237.0 22 358.0 23 548.0 24 810.0 25 1381.0 26 1883.0 27 2317.0 28 2788.0 29 3135.0 30 3892.0 31 4822.0 32 6326.0 33 8926.0 34 17842.0 35 26242.0 36 21971.0 37 34633.0 38 64925.0 39 144102.0 40 218.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.96851410380655 21.043126676884942 24.205232024799926 11.78312719450859 2 32.552488475684626 23.969138127870295 24.71135292442982 18.767020472015254 3 33.306781157348965 23.67984402274093 25.598214773554034 17.415160046356075 4 29.387507440839933 25.616906738597184 24.26447117555206 20.731114645010827 5 25.302019019793352 29.990251421308265 24.208970417808555 20.498759141089828 6 26.74331330896668 34.129802756633495 23.074799492728825 16.052084441671006 7 80.97733095993306 6.031465766384946 8.227340305915575 4.763862967766426 8 81.46447232582683 5.219946914819277 8.25264635089707 5.06293440845682 9 74.92458511026821 8.009363236643154 10.472389091944338 6.593662561144294 10 40.23316069626132 27.13066833839933 17.581087182200648 15.055083783138699 11 29.995427657781754 25.30719525626684 24.211558536045295 20.48581854990611 12 28.090860204231287 22.721952706452754 28.399421411789742 20.787765677526217 13 24.495963973394144 24.544275513813364 29.97961137966832 20.980149133124176 14 20.64685701797017 27.511409287893645 29.209502419890555 22.632231274245633 15 20.103927325639912 25.995922275933665 32.35722933315696 21.542921065269464 16 22.82806555415925 25.406118886649047 30.55216064737464 21.21365491181706 17 21.922799308684862 24.98109235843712 28.234356982024085 24.861751350853936 18 23.235837960792885 25.950773991137133 28.67893818135807 22.13444986671191 19 24.50631644634112 26.929945390705207 26.243231351889186 22.320506811064494 20 26.24668217620484 25.764141909398607 27.644266024046495 20.344909890350056 21 24.825517695539524 26.257034649151816 27.541316431962688 21.376131223345975 22 23.747710234282216 24.74643630497235 26.631449087400753 24.87440437334468 23 22.881840899744926 26.623109595304577 27.210324866352448 23.284724638598046 24 22.954595779066725 24.746148736279377 28.38791866407088 23.911336820583017 25 22.75070957574991 26.83562285941054 26.01260126012601 24.401066304713538 26 21.934302056403723 28.747379530285293 26.18226678897922 23.136051624331763 27 24.016011824824655 26.607580885884115 26.98314559890494 22.39326169038629 28 20.326217925306906 27.254322876377095 28.719197798374086 23.700261399941912 29 22.030349999856217 26.86351702262878 26.900325815329136 24.205807162185867 30 23.740233448264956 25.42452328299923 26.920168055144174 23.915075213591646 31 23.86618853578649 27.727373376315267 25.531211268091663 22.87522681980658 32 23.058120508536476 27.392643417696398 24.78583321590945 24.76340285785767 33 22.621591232605688 26.571347230569703 24.50602887764815 26.30103265917646 34 21.128247010004515 27.133831594022023 26.832747172480826 24.905174223492637 35 22.0274743129265 26.15207207621721 25.23156469001533 26.588888920840965 36 22.578168359966988 28.26800251910175 24.839033424109182 24.314795696822078 37 22.395849808623037 27.32707775569889 26.774658296500576 23.502414139177496 38 20.759296376922038 27.087533034453603 25.032854723171997 27.120315865452362 39 22.279096919276594 24.797911101014254 25.480311609435702 27.442680370273447 40 22.346963130817873 25.65371553129754 27.67963697328199 24.319684364602594 41 20.26985446148449 26.33467819625413 26.42094880414559 26.974518538115795 42 21.839404387723118 25.365859269633035 26.753665781913654 26.041070560730194 43 22.673353597340565 24.619906080064876 27.51198442527959 25.19475589731497 44 22.094190249695895 24.837595580644326 26.565883425403243 26.502330744256536 45 22.274208251496074 24.507754289805977 25.488075964145935 27.729961494552015 46 22.097928642704527 25.679309144972006 25.97176650572405 26.25099570659941 47 20.861383262926932 25.486350551988107 28.697342577708252 24.95492360737671 48 20.912570490275865 24.110046787426345 28.121342485686267 26.856040236611523 49 21.76291111539269 23.96856299048435 28.89403956370078 25.37448633042218 50 20.09645053962265 24.1721616251082 28.575988589274264 27.15539924599489 51 20.340883928648456 23.219158976600536 26.964166065168815 29.475791029582194 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 137.0 1 345.5 2 554.0 3 1034.0 4 1514.0 5 1102.5 6 691.0 7 666.5 8 642.0 9 650.5 10 659.0 11 653.5 12 648.0 13 653.5 14 659.0 15 678.5 16 698.0 17 717.0 18 736.0 19 770.5 20 805.0 21 889.0 22 973.0 23 1085.0 24 1197.0 25 1374.0 26 2037.0 27 2523.0 28 3130.5 29 3738.0 30 4167.5 31 4597.0 32 5409.0 33 6221.0 34 7233.5 35 8246.0 36 8629.0 37 9012.0 38 10119.5 39 11227.0 40 12642.5 41 14058.0 42 15401.0 43 16744.0 44 17944.5 45 19145.0 46 21295.5 47 23446.0 48 25714.0 49 27982.0 50 31276.0 51 34570.0 52 34641.0 53 34712.0 54 30444.0 55 26176.0 56 23576.0 57 20976.0 58 18909.0 59 16842.0 60 16192.0 61 15542.0 62 14203.5 63 12865.0 64 11326.0 65 9787.0 66 7980.0 67 6173.0 68 5223.5 69 4274.0 70 3356.5 71 2439.0 72 2255.0 73 2071.0 74 1805.5 75 1185.5 76 831.0 77 591.0 78 351.0 79 225.0 80 99.0 81 85.5 82 72.0 83 42.5 84 13.0 85 8.5 86 4.0 87 2.0 88 0.0 89 1.5 90 3.0 91 1.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 347743.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.78860232913464 #Duplication Level Percentage of deduplicated Percentage of total 1 71.3110045682032 24.80810179614325 2 10.086099554226257 7.017626128880789 3 3.718956591052258 3.881319057762937 4 2.020067562425067 2.8110130842876013 5 1.4652390705297764 2.548680967088563 6 1.1505402888355716 2.4015413143169053 7 1.0071182804605072 2.4525366150139734 8 0.8853446375652949 2.463992201639271 9 0.8000349035337735 2.5048886497617637 >10 7.4413040696981705 44.53472419879905 >50 0.08281918367401181 1.9966464067187186 >100 0.028158522449164017 1.5710985361958405 >500 0.0024845755102203544 0.6958010205231899 >1k 8.281918367401182E-4 0.3120300228681634 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC 1083 0.31143689448817086 Illumina PCR Primer Index 7 (95% over 22bp) CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC 973 0.2798043382613022 RNA PCR Primer, Index 16 (96% over 25bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 731 0.21021271456219104 No Hit CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT 711 0.20446134070276037 RNA PCR Primer, Index 16 (96% over 26bp) TCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC 374 0.10755069117135356 Illumina PCR Primer Index 7 (95% over 22bp) CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCC 351 0.10093661123300827 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.2081997337113903 0.0 2 0.0 0.0 0.0 1.0154050548824851 0.0 3 0.0 0.0 0.0 1.3573242308256386 0.0 4 0.0 0.0 0.0 1.8085195100979747 0.0 5 0.0 0.0 0.0 3.2478008184205 0.0 6 0.0 0.0 0.0 4.103030111317841 0.0 7 0.0 0.0 0.0 4.957396698136267 0.0 8 0.0 0.0 0.0 6.116586099504519 0.0 9 0.0 0.0 0.0 6.543913177260218 0.0 10 0.0 0.0 0.0 7.539763561020639 0.0 11 0.0 0.0 0.0 9.176316992721636 0.0 12 0.0 0.0 0.0 10.404235311710085 0.0 13 0.0 0.0 0.0 10.903454562708667 0.0 14 0.0 0.0 0.0 11.08951150706125 0.0 15 0.0 0.0 0.0 11.48348061643225 0.0 16 0.0 0.0 0.0 12.266817736086708 0.0 17 2.875686929715336E-4 0.0 0.0 13.176397511955669 0.0 18 2.875686929715336E-4 0.0 0.0 14.075912383570625 0.0 19 2.875686929715336E-4 0.0 0.0 14.69188452391565 0.0 20 2.875686929715336E-4 0.0 0.0 15.248329944815568 0.0 21 2.875686929715336E-4 0.0 0.0 15.943095907034794 0.0 22 2.875686929715336E-4 0.0 0.0 16.69652588262021 0.0 23 2.875686929715336E-4 0.0 0.0 17.417748164592815 0.0 24 2.875686929715336E-4 0.0 0.0 18.003238023482858 0.0 25 2.875686929715336E-4 0.0 0.0 18.543867166269344 0.0 26 2.875686929715336E-4 0.0 0.0 19.028132845233404 0.0 27 2.875686929715336E-4 0.0 0.0 19.49226871568946 0.0 28 2.875686929715336E-4 0.0 0.0 20.00241557702096 0.0 29 2.875686929715336E-4 0.0 0.0 20.520326793062694 0.0 30 2.875686929715336E-4 0.0 0.0 21.094889041619815 0.0 31 2.875686929715336E-4 0.0 0.0 21.59468343000434 0.0 32 2.875686929715336E-4 0.0 0.0 22.11000652780933 0.0 33 2.875686929715336E-4 0.0 0.0 22.60692522926414 0.0 34 2.875686929715336E-4 0.0 0.0 23.085727103061743 0.0 35 2.875686929715336E-4 0.0 0.0 23.63843413095303 0.0 36 2.875686929715336E-4 0.0 0.0 24.1782005676606 0.0 37 2.875686929715336E-4 0.0 0.0 24.671668444799753 0.0 38 2.875686929715336E-4 0.0 0.0 25.14414380735198 0.0 39 2.875686929715336E-4 0.0 0.0 25.66694369117423 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCACGG 30 2.1611177E-6 45.000004 2 TGCGATG 25 3.8851744E-5 45.000004 1 CACGACG 35 1.2086639E-7 45.0 26 ACGGGTA 35 1.2086639E-7 45.0 5 TCGATAG 20 7.0264924E-4 45.0 1 TATTGCG 20 7.0264924E-4 45.0 1 GCCGACC 20 7.0264924E-4 45.0 14 TATTAGG 20 7.0264924E-4 45.0 2 GTTCACG 20 7.0264924E-4 45.0 1 CGGCTAT 20 7.0264924E-4 45.0 35 CGGTAGT 40 6.7921064E-9 45.0 12 ATACAGG 35 1.2086639E-7 45.0 2 AATCGCG 20 7.0264924E-4 45.0 1 TAGTACG 20 7.0264924E-4 45.0 1 ATTTCCG 55 1.8189894E-12 45.0 32 AGACACG 90 0.0 42.5 24 ACACGAC 80 0.0 42.1875 26 CGACCCG 60 3.6379788E-12 41.250004 32 CATGCAT 90 0.0 40.0 25 CGCCGGT 45 1.9215804E-8 40.0 28 >>END_MODULE