##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550358_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	376706
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28086890041571	33.0	31.0	34.0	30.0	34.0
2	32.4173227928411	34.0	31.0	34.0	30.0	34.0
3	32.40105281041449	34.0	31.0	34.0	30.0	34.0
4	35.90493116647996	37.0	35.0	37.0	35.0	37.0
5	36.00540739993523	37.0	35.0	37.0	35.0	37.0
6	36.064087113027135	37.0	35.0	37.0	35.0	37.0
7	36.1721581286202	37.0	36.0	37.0	35.0	37.0
8	36.12620717482599	37.0	37.0	37.0	35.0	37.0
9	37.97376999569956	39.0	38.0	39.0	35.0	39.0
10	37.64231522725945	39.0	37.0	39.0	35.0	39.0
11	37.52788646849267	39.0	37.0	39.0	35.0	39.0
12	37.49721533503581	39.0	37.0	39.0	35.0	39.0
13	37.48323891841383	39.0	37.0	39.0	35.0	39.0
14	38.75148258854385	40.0	38.0	41.0	35.0	41.0
15	38.85263574246229	40.0	38.0	41.0	35.0	41.0
16	38.891488322458365	40.0	38.0	41.0	35.0	41.0
17	38.85838558451418	40.0	38.0	41.0	35.0	41.0
18	38.82200442785621	40.0	38.0	41.0	35.0	41.0
19	38.778893885417276	40.0	38.0	41.0	35.0	41.0
20	38.74389577017621	40.0	38.0	41.0	35.0	41.0
21	38.6726465731897	40.0	38.0	41.0	35.0	41.0
22	38.64120295402781	40.0	38.0	41.0	35.0	41.0
23	38.61091408153839	40.0	38.0	41.0	35.0	41.0
24	38.61485349317505	40.0	38.0	41.0	35.0	41.0
25	38.52631229659204	40.0	38.0	41.0	35.0	41.0
26	38.563009349466164	40.0	38.0	41.0	35.0	41.0
27	38.56926621821792	40.0	38.0	41.0	35.0	41.0
28	38.520915515017016	40.0	38.0	41.0	35.0	41.0
29	38.50849999734541	40.0	38.0	41.0	35.0	41.0
30	38.42945692396723	40.0	38.0	41.0	35.0	41.0
31	38.38016915047809	40.0	38.0	41.0	34.0	41.0
32	38.31073569308692	40.0	38.0	41.0	34.0	41.0
33	38.19470621651898	40.0	37.0	41.0	34.0	41.0
34	38.135466384926175	40.0	37.0	41.0	34.0	41.0
35	38.07625044464383	40.0	37.0	41.0	34.0	41.0
36	37.9381241604859	40.0	37.0	41.0	33.0	41.0
37	37.929494088227955	40.0	37.0	41.0	34.0	41.0
38	37.88352189771334	40.0	37.0	41.0	33.0	41.0
39	37.77470494231576	40.0	37.0	41.0	33.0	41.0
40	37.73550461102292	40.0	37.0	41.0	33.0	41.0
41	37.709627667199356	40.0	37.0	41.0	33.0	41.0
42	37.634311638253706	40.0	36.0	41.0	33.0	41.0
43	37.568007942533434	40.0	36.0	41.0	33.0	41.0
44	37.53614489814338	40.0	36.0	41.0	33.0	41.0
45	37.46199423423041	40.0	36.0	41.0	33.0	41.0
46	37.39199269456818	40.0	36.0	41.0	33.0	41.0
47	37.3015056834773	40.0	35.0	41.0	32.0	41.0
48	37.25910126199211	39.0	35.0	41.0	32.0	41.0
49	37.19390983950348	39.0	35.0	41.0	32.0	41.0
50	37.09255493674112	39.0	35.0	41.0	32.0	41.0
51	36.14000838850456	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	1.0
10	5.0
11	5.0
12	6.0
13	5.0
14	9.0
15	5.0
16	13.0
17	22.0
18	30.0
19	69.0
20	148.0
21	215.0
22	344.0
23	548.0
24	875.0
25	1257.0
26	1860.0
27	2227.0
28	2744.0
29	3271.0
30	3967.0
31	5186.0
32	6673.0
33	9244.0
34	17718.0
35	26968.0
36	22012.0
37	34166.0
38	71295.0
39	165569.0
40	244.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.487557936427876	21.9614234973693	27.39536933311389	11.155649233088933
2	30.412045467818405	24.644417662580366	27.879566558536368	17.063970311064864
3	30.687060997170207	24.474258440269068	28.42614665017281	16.41253391238791
4	28.406502683790542	26.725350804075326	26.80419212860958	18.063954383524553
5	24.6130934999708	30.441776876396975	25.722446682558815	19.22268294107341
6	25.351334993336977	34.118649556948924	24.55761256789114	15.972402881822962
7	82.61721342373097	4.93753749608448	7.678401724421698	4.76684735576285
8	83.96229420290624	3.843050017785753	7.213317547371159	4.981338231936841
9	77.6897633698428	7.786443539524192	9.418750962288893	5.10504212834412
10	35.019351961476595	39.07078729831752	15.272387485200662	10.63747325500523
11	23.092544318380913	23.78963966594639	33.81761904509087	19.300196970581833
12	23.38269101102717	22.081676426709425	35.110935318258804	19.42469724400461
13	21.55314754742425	25.177193885948192	35.098724204021174	18.170934362606385
14	17.927773913874482	29.472586048536524	32.23070511220952	20.368934925379474
15	17.12714955429433	27.93982575270901	35.93226548024189	19.000759212754776
16	19.965437237527407	27.694276172930614	32.81259125153302	19.52769533800895
17	20.00605246531778	26.512983599942665	29.52381963653353	23.95714429820603
18	20.030209234787872	26.88701533822132	32.170445918036876	20.912329508953935
19	20.394153530870227	28.265013034037157	30.469915530944558	20.87091790414806
20	22.22767887955063	28.277224148274783	30.170212314112334	19.324884658062256
21	21.894793287072677	27.716840188369712	31.605549155043988	18.782817369513626
22	20.66332896210838	25.33753112506836	29.94722674977303	24.05191316305023
23	18.342686338948678	27.55862662129087	31.160639862386052	22.9380471773744
24	20.50352264099855	25.84747787399192	31.861982554034178	21.78701693097535
25	18.92961619937033	28.2281142323191	29.48479716277415	23.357472405536413
26	19.53539364915876	28.773632487934886	29.486124457799985	22.20484940510637
27	20.338141680780236	27.926287343445548	29.830424787500064	21.905146188274145
28	17.35252424968012	27.275647321784096	32.88161059287614	22.49021783565964
29	21.79790075018715	24.678130956236426	30.068806974138983	23.45516131943744
30	21.747198080200477	26.054004980010937	29.776802068456572	22.42199487133202
31	23.235892181170463	26.463608224981815	28.09007554963287	22.210424044214854
32	25.22975476897103	26.810828603738724	26.61359256290051	21.34582406438974
33	22.474821213360023	27.18671855505354	27.739935121819137	22.598525109767298
34	22.655333336872786	24.714233380938982	30.371961158038363	22.25847212414987
35	23.556566659410787	24.691138447489553	28.370665718093157	23.381629175006506
36	23.61390580452661	26.061172373150416	29.279597351780968	21.045324470542013
37	22.48942145864414	25.75961094328203	31.26151428434907	20.489453313724763
38	22.14936847302671	27.531284343758795	28.808407617611614	21.510939565602886
39	23.69407442408669	25.783236794741786	29.39958482211592	21.1231039590556
40	24.157300388101067	25.106316331568916	29.51054668627524	21.22583659405478
41	22.66011159896577	24.89501096345691	28.09538472973619	24.34949270784113
42	23.01980855096547	24.17083879736452	29.57717689657186	23.23217575509814
43	22.47216662330836	24.05403683509156	30.628129098023393	22.84566744357669
44	21.761798325484598	24.84882109655806	29.38817008489379	24.001210493063557
45	23.073165811003808	24.31418666015407	27.694010713925447	24.918636814916674
46	25.103130823506923	24.736797396378076	27.854878871055945	22.305192909059055
47	21.671807722733377	24.067575244355012	31.40725127818511	22.8533657547265
48	21.642872691170304	22.925039686121274	31.04251060508726	24.38957701762117
49	22.566935488152566	22.160517751243674	32.850817348276905	22.421729412326854
50	21.840639650018847	22.486235950582152	30.889075300101403	24.784049099297594
51	20.771370777210876	22.6932939746115	28.75186484951129	27.78347039866633
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	126.0
1	222.0
2	318.0
3	907.0
4	1496.0
5	1006.5
6	517.0
7	503.0
8	489.0
9	573.5
10	658.0
11	631.5
12	605.0
13	660.0
14	715.0
15	705.0
16	695.0
17	753.0
18	811.0
19	912.5
20	1014.0
21	1147.0
22	1280.0
23	1475.0
24	1670.0
25	2073.5
26	3019.0
27	3561.0
28	4078.0
29	4595.0
30	6214.0
31	7833.0
32	8758.5
33	9684.0
34	11373.5
35	13063.0
36	13700.5
37	14338.0
38	15532.5
39	16727.0
40	18572.0
41	20417.0
42	22363.5
43	24310.0
44	25872.0
45	27434.0
46	29267.5
47	31101.0
48	34427.0
49	37753.0
50	40786.0
51	43819.0
52	41177.0
53	38535.0
54	30696.0
55	22857.0
56	18852.0
57	14847.0
58	12578.5
59	10310.0
60	8984.5
61	7659.0
62	6805.5
63	5952.0
64	4810.0
65	3668.0
66	2942.0
67	2216.0
68	1765.5
69	1315.0
70	1156.0
71	997.0
72	713.5
73	430.0
74	320.5
75	154.0
76	97.0
77	75.5
78	54.0
79	47.5
80	41.0
81	22.5
82	4.0
83	4.5
84	5.0
85	3.0
86	1.0
87	0.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	376706.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.78160621152832
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.15039131676637	26.88176066526211
2	11.135351308172396	8.414229163047922
3	4.013200344611337	4.548754652042258
4	2.1793545002551244	3.293580540958448
5	1.4935930157718438	2.82151715810904
6	1.1631576003393505	2.6367577450780546
7	0.991399077989708	2.6219654694155583
8	0.8333767783428578	2.518905061214558
9	0.7675682169893168	2.609996410329776
>10	6.1774734217986715	38.73815058281427
>50	0.06201354717587708	1.585233162476352
>100	0.029597374788486785	1.8248552394378428
>500	0.0021140981991776275	0.7034239192580654
>1k	0.0014093987994517516	0.8008702305557385
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	1656	0.4396001125546182	No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	1352	0.3589005749842052	No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT	980	0.2601498250625156	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	835	0.22165826931346994	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	827	0.21953459727214325	No Hit
ACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	500	0.1327295025829161	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCC	449	0.11919109331945868	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6545900516583223E-4	0.0	0.0	0.2718300212898122	0.0
2	2.6545900516583223E-4	0.0	0.0	1.4767484457375248	0.0
3	2.6545900516583223E-4	0.0	0.0	1.9893497847127468	0.0
4	2.6545900516583223E-4	0.0	0.0	2.6622883628081317	0.0
5	2.6545900516583223E-4	0.0	0.0	4.568284019898807	0.0
6	2.6545900516583223E-4	0.0	0.0	5.556853355136366	0.0
7	2.6545900516583223E-4	0.0	0.0	6.653464505476419	0.0
8	2.6545900516583223E-4	0.0	0.0	8.359834990682389	0.0
9	2.6545900516583223E-4	0.0	0.0	8.997202062085552	0.0
10	2.6545900516583223E-4	0.0	0.0	10.331399022049025	0.0
11	2.6545900516583223E-4	0.0	0.0	12.365611378634798	0.0
12	2.6545900516583223E-4	0.0	0.0	14.03933040620537	0.0
13	5.309180103316645E-4	0.0	0.0	14.615376447415226	0.0
14	5.309180103316645E-4	0.0	0.0	14.833052831651209	0.0
15	7.963770154974968E-4	0.0	0.0	15.195669832707734	0.0
16	7.963770154974968E-4	0.0	0.0	16.070091795723986	0.0
17	7.963770154974968E-4	0.0	0.0	17.34827690559747	0.0
18	7.963770154974968E-4	0.0	0.0	18.667608161271655	0.0
19	7.963770154974968E-4	0.0	0.0	19.49132745430123	0.0
20	7.963770154974968E-4	0.0	0.0	20.22585252159509	0.0
21	0.001061836020663329	0.0	0.0	21.10744187775082	0.0
22	0.001061836020663329	0.0	0.0	22.11246967130866	0.0
23	0.001061836020663329	0.0	0.0	23.079802286132953	0.0
24	0.001061836020663329	0.0	0.0	23.820432910545623	0.0
25	0.001061836020663329	0.0	0.0	24.433112294468366	0.0
26	0.001061836020663329	0.0	0.0	25.039420662267126	0.0
27	0.001061836020663329	0.0	0.0	25.615201244471816	0.0
28	0.001061836020663329	0.0	0.0	26.20160018688314	0.0
29	0.001061836020663329	0.0	0.0	26.879051568066345	0.0
30	0.001061836020663329	0.0	0.0	27.616496684417026	0.0
31	0.001061836020663329	0.0	0.0	28.287046131465917	0.0
32	0.001061836020663329	0.0	0.0	28.88485981109937	0.0
33	0.0013272950258291611	0.0	0.0	29.471258753510696	0.0
34	0.0013272950258291611	0.0	0.0	30.070134269164814	0.0
35	0.0013272950258291611	0.0	0.0	30.70909409459897	0.0
36	0.0013272950258291611	0.0	0.0	31.34301019893498	0.0
37	0.0013272950258291611	0.0	0.0	31.954096828826724	0.0
38	0.0013272950258291611	0.0	0.0	32.55615785254283	0.0
39	0.0013272950258291611	0.0	0.0	33.12052369752539	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTGG	35	1.2090277E-7	45.000004	6
TGAACGG	30	2.1616015E-6	45.000004	2
AGTTTCG	20	7.027243E-4	45.0	12
CCGATGA	20	7.027243E-4	45.0	18
TAGCGAA	20	7.027243E-4	45.0	20
ATTCGCG	25	3.8857965E-5	45.0	1
TCGGCCG	20	7.027243E-4	45.0	2
CTAACGG	25	3.8857965E-5	45.0	2
GTATGCG	25	3.8857965E-5	45.0	1
GCGGGTG	20	7.027243E-4	45.0	5
GTTCCGA	25	3.8857965E-5	45.0	9
GCGATAT	20	7.027243E-4	45.0	9
TTACGAG	25	3.8857965E-5	45.0	1
TCGAGCG	20	7.027243E-4	45.0	1
TATACGG	20	7.027243E-4	45.0	2
GGTTCCG	25	3.8857965E-5	45.0	8
TCAACGG	50	2.1827873E-11	45.0	2
TTAGGCG	65	0.0	44.999996	1
CGACAGG	100	0.0	42.75	2
GTAGGGT	65	0.0	41.53846	4
>>END_MODULE