##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550357_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	596896
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37137625314963	33.0	31.0	34.0	30.0	34.0
2	32.53270418967458	34.0	31.0	34.0	31.0	34.0
3	32.45803624081917	34.0	31.0	34.0	30.0	34.0
4	35.9765134964885	37.0	35.0	37.0	35.0	37.0
5	36.06622929287514	37.0	35.0	37.0	35.0	37.0
6	36.10882632820458	37.0	35.0	37.0	35.0	37.0
7	36.17160108293572	37.0	37.0	37.0	35.0	37.0
8	36.128622071516645	37.0	37.0	37.0	35.0	37.0
9	37.97127807859326	39.0	38.0	39.0	35.0	39.0
10	37.706245309065565	39.0	38.0	39.0	35.0	39.0
11	37.590184889830056	39.0	37.0	39.0	35.0	39.0
12	37.53674676995657	39.0	37.0	39.0	35.0	39.0
13	37.52700637967083	39.0	37.0	39.0	35.0	39.0
14	38.82436638878465	40.0	38.0	41.0	35.0	41.0
15	38.914055379831666	40.0	38.0	41.0	35.0	41.0
16	38.95131815257599	40.0	38.0	41.0	35.0	41.0
17	38.915687154881255	40.0	38.0	41.0	35.0	41.0
18	38.78930500455691	40.0	38.0	41.0	35.0	41.0
19	38.68637417573581	40.0	37.0	41.0	35.0	41.0
20	38.56648561893529	40.0	37.0	41.0	35.0	41.0
21	38.48253967190264	40.0	37.0	41.0	35.0	41.0
22	38.46504583713076	40.0	37.0	41.0	35.0	41.0
23	38.414377043907145	40.0	37.0	41.0	35.0	41.0
24	38.35954672170696	40.0	37.0	41.0	34.0	41.0
25	38.280899855251164	40.0	37.0	41.0	34.0	41.0
26	38.29288351739667	40.0	37.0	41.0	34.0	41.0
27	38.285835388409375	40.0	37.0	41.0	34.0	41.0
28	38.21768113708251	40.0	37.0	41.0	34.0	41.0
29	38.17525498579317	40.0	37.0	41.0	34.0	41.0
30	38.08322722886399	40.0	36.0	41.0	34.0	41.0
31	37.96437402830644	40.0	36.0	41.0	34.0	41.0
32	37.79445330509837	40.0	36.0	41.0	33.0	41.0
33	37.60026872352973	40.0	36.0	41.0	33.0	41.0
34	37.38504697635769	40.0	36.0	41.0	33.0	41.0
35	37.25348301881735	40.0	36.0	41.0	33.0	41.0
36	37.148017410068086	40.0	36.0	41.0	32.0	41.0
37	37.09864532514877	40.0	36.0	41.0	32.0	41.0
38	37.02829806197394	40.0	35.0	41.0	32.0	41.0
39	36.92977168551975	40.0	35.0	41.0	31.0	41.0
40	36.82508845762076	40.0	35.0	41.0	31.0	41.0
41	36.745741301667294	40.0	35.0	41.0	31.0	41.0
42	36.68198480137244	40.0	35.0	41.0	31.0	41.0
43	36.63791682303115	40.0	35.0	41.0	31.0	41.0
44	36.57167077681874	39.0	35.0	41.0	31.0	41.0
45	36.44934125877875	39.0	35.0	41.0	30.0	41.0
46	36.35108796172197	39.0	35.0	41.0	30.0	41.0
47	36.2784220366697	39.0	35.0	41.0	30.0	41.0
48	36.222738969602744	39.0	35.0	41.0	30.0	41.0
49	36.15651302739506	39.0	35.0	41.0	30.0	41.0
50	36.07453894815848	39.0	35.0	41.0	30.0	41.0
51	35.20094790382244	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	10.0
10	20.0
11	8.0
12	11.0
13	12.0
14	9.0
15	15.0
16	25.0
17	64.0
18	114.0
19	200.0
20	336.0
21	566.0
22	911.0
23	1525.0
24	2543.0
25	4421.0
26	6334.0
27	7360.0
28	7174.0
29	6956.0
30	7669.0
31	8797.0
32	11047.0
33	15048.0
34	28191.0
35	41880.0
36	36260.0
37	54420.0
38	107193.0
39	247452.0
40	319.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.62347879697636	21.513630515198628	25.738822173376935	14.12406851444808
2	34.61423765614111	23.74551010561304	24.70480619739452	16.93544604085134
3	30.50732455905216	23.836983327078755	29.409813434836217	16.245878679032863
4	26.915744116227952	25.456863507210635	29.44382270948373	18.18356966707768
5	24.727423202701978	29.320685680587573	27.464248378276952	18.487642738433497
6	24.990115531013778	33.363265962579746	26.390191926231704	15.25642658017477
7	79.00773334048142	5.275960971425508	11.10327427223503	4.613031415858039
8	81.09402643006487	4.4233836380206935	10.437664182705195	4.044925749209242
9	74.48483487910792	7.796333029539484	12.485592129952286	5.233239961400311
10	37.58577708679569	32.38453599957111	17.72184769206026	12.307839221572937
11	27.03134214335496	24.635949981236262	30.386532997373077	17.946174878035702
12	25.573131667828232	22.34392591004128	32.52559909934059	19.5573433227899
13	22.93029271430869	23.311263603709858	34.17027421862435	19.5881694633571
14	19.633403473972017	27.414155899855253	32.32723958612556	20.625201040047177
15	19.05524580496435	26.329544845333192	35.08617916689005	19.529030182812416
16	21.92827561250201	26.891619310566668	32.40698547150592	18.7731196054254
17	21.947206883611216	26.048926446148073	30.502633624618024	21.50123304562269
18	22.826589556639682	26.379134723636948	30.460750281456068	20.3335254382673
19	23.113239157240123	28.027328043746312	28.72158633999893	20.137846459014636
20	24.658231919798425	27.178939044657696	29.12550930145285	19.03731973409103
21	23.351136546400042	27.147442770599906	30.37849139548598	19.122929287514072
22	22.638784645901463	25.010722135849463	30.132552404438968	22.21794081381011
23	21.870644132311156	26.56023829946925	30.162373344770277	21.406744223449312
24	21.972001822763094	25.555038063582263	31.152160510373665	21.320799603280975
25	21.849702460730178	27.281134401972874	29.026329544845332	21.842833592451615
26	21.52602798477457	28.06116978502118	28.622909183509353	21.789893046694903
27	21.942683482549725	26.765969281080793	29.55338953519541	21.737957701174075
28	19.66154908057685	27.589060740899583	30.1645512786147	22.584838899908863
29	21.581816597866297	25.92562188387927	29.524573795099982	22.96798772315445
30	22.97653192515949	25.017758537500672	29.123498900981076	22.882210636358764
31	22.768120409585592	27.263040797726905	27.118627030504477	22.850211762183026
32	23.07537661502171	27.241931592773284	27.534947461534337	22.14774433067067
33	22.698594059936738	27.63697528547687	27.65523642309548	22.009194231490913
34	20.744317267999783	27.856946603763472	28.707681070069157	22.691055058167585
35	20.649493379081115	27.96718356296574	27.887940277703315	23.495382780249827
36	20.552156489572724	30.384522596901302	27.332232080630465	21.731088832895512
37	21.311250201040046	29.25149439768402	28.048772315445238	21.388483085830696
38	20.637766042995764	29.815411730016617	27.857784270626706	21.689037956360906
39	21.66792875140728	27.946744491502706	28.396404063689484	21.988922693400525
40	22.54178282313837	27.460060043960755	28.97003833163566	21.02811880126521
41	19.837291588484426	28.3722792580282	28.546513965581944	23.243915187905433
42	21.444439232295075	27.463410711413715	28.194861416394147	22.897288639897067
43	21.804300916742612	25.978394896263335	29.341627352168555	22.875676834825498
44	21.931291213209672	26.318487642738436	28.18346914705409	23.5667519969978
45	21.648159813434837	25.639977483514713	27.740008309655284	24.971854393395166
46	21.5853347986919	27.1827923122286	27.043069479440305	24.1888034096392
47	20.574605961507533	27.36004262049	28.670488661341338	23.394862756661126
48	21.002653728622743	25.83347852892296	29.638831555245805	23.525036187208492
49	21.619679140084706	25.26788586286388	29.777549187798208	23.3348858092532
50	19.51713531335442	25.709001233045626	30.26775183616576	24.506111617434193
51	19.793397844850695	25.44044523669115	28.770171018066797	25.99598590039136
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	260.0
1	453.5
2	647.0
3	4545.5
4	8444.0
5	5611.0
6	2778.0
7	2796.0
8	2814.0
9	2859.5
10	2905.0
11	2951.5
12	2998.0
13	2965.0
14	2932.0
15	2812.0
16	2692.0
17	2640.0
18	2588.0
19	2517.0
20	2446.0
21	2574.5
22	2703.0
23	2735.0
24	2767.0
25	3124.5
26	4040.0
27	4598.0
28	5156.0
29	5714.0
30	7233.0
31	8752.0
32	10154.5
33	11557.0
34	13215.0
35	14873.0
36	16350.0
37	17827.0
38	20141.5
39	22456.0
40	25824.5
41	29193.0
42	32916.0
43	36639.0
44	43493.0
45	50347.0
46	55946.0
47	61545.0
48	59578.0
49	57611.0
50	52321.5
51	47032.0
52	42245.5
53	37459.0
54	35161.0
55	32863.0
56	30080.5
57	27298.0
58	25506.0
59	23714.0
60	22535.5
61	21357.0
62	19018.5
63	16680.0
64	13694.5
65	10709.0
66	8802.0
67	6895.0
68	5605.0
69	4315.0
70	3544.5
71	2774.0
72	2304.5
73	1835.0
74	1399.0
75	874.5
76	786.0
77	609.5
78	433.0
79	276.5
80	120.0
81	89.0
82	58.0
83	42.0
84	26.0
85	17.0
86	8.0
87	4.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	596896.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.37248972961996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.78783296916609	27.20262540081453
2	9.32700384072719	6.9714671049140575
3	3.7411286079353783	4.19445871431757
4	2.2110055682599232	3.3052313156770605
5	1.605608335989362	3.0002790523277314
6	1.3072932769860661	2.931408274065778
7	1.0751698699072292	2.8127242444493326
8	0.92945510414688	2.7788841067097696
9	0.7812270000297866	2.627675823160351
>10	6.142161804542208	39.07612497700809
>50	0.06335619876373062	1.6344391426852563
>100	0.023814741374156716	1.3746551751610936
>500	0.004493347429086173	1.207567232419182
>1k	0.0	0.0
>5k	4.4933474290861735E-4	0.8824594362901962
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5255	0.8803878732643542	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTT	962	0.16116710448721386	No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	846	0.14173323326006543	No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	798	0.1336916313729695	No Hit
CGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG	780	0.13067603066530853	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCT	717	0.12012142818849515	No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	681	0.11409022677317322	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC	640	0.10722135849461213	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	631	0.10571355814078165	No Hit
CGTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCT	627	0.10504342465019031	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.350667452956629E-4	0.0	0.0	0.14022543290623493	0.0
2	3.350667452956629E-4	0.0	0.0	0.5141599206561948	0.0
3	3.350667452956629E-4	0.0	0.0	0.8559280008577709	0.0
4	3.350667452956629E-4	0.0	0.0	1.3069278400257331	0.0
5	5.026001179434943E-4	0.0	0.0	2.468939312711092	0.0
6	5.026001179434943E-4	0.0	0.0	3.579350506620919	0.0
7	5.026001179434943E-4	0.0	0.0	4.523233528118801	0.0
8	5.026001179434943E-4	0.0	0.0	5.810727496917386	0.0
9	5.026001179434943E-4	0.0	0.0	6.481531120999303	0.0
10	5.026001179434943E-4	0.0	0.0	7.527609499812363	0.0
11	5.026001179434943E-4	0.0	0.0	8.865531013777945	0.0
12	5.026001179434943E-4	0.0	0.0	9.974937007451885	0.0
13	5.026001179434943E-4	0.0	0.0	10.482228059829518	0.0
14	5.026001179434943E-4	0.0	0.0	10.75631265748137	0.0
15	5.026001179434943E-4	0.0	0.0	11.05988312871924	0.0
16	5.026001179434943E-4	0.0	0.0	11.575550849729266	0.0
17	5.026001179434943E-4	0.0	0.0	12.25741167640594	0.0
18	5.026001179434943E-4	0.0	0.0	13.009301452849408	0.0
19	5.026001179434943E-4	0.0	0.0	13.51441457138262	0.0
20	5.026001179434943E-4	0.0	0.0	13.992387283546883	0.0
21	5.026001179434943E-4	0.0	0.0	14.574900820243393	0.0
22	5.026001179434943E-4	0.0	0.0	15.20633410175307	0.0
23	5.026001179434943E-4	0.0	0.0	15.81582051144588	0.0
24	5.026001179434943E-4	0.0	0.0	16.30585562644079	0.0
25	5.026001179434943E-4	0.0	0.0	16.72820725888597	0.0
26	5.026001179434943E-4	0.0	0.0	17.155919959255883	0.0
27	5.026001179434943E-4	0.0	0.0	17.58631319358816	0.0
28	5.026001179434943E-4	0.0	0.0	18.033459765185224	0.0
29	5.026001179434943E-4	0.0	0.0	18.515620811665684	0.0
30	5.026001179434943E-4	0.0	0.0	19.089422612984507	0.0
31	5.026001179434943E-4	0.0	0.0	19.601907199914223	0.0
32	5.026001179434943E-4	0.0	0.0	20.089429314319414	0.0
33	5.026001179434943E-4	0.0	0.0	20.57092022730928	0.0
34	6.701334905913258E-4	0.0	0.0	21.02979413499169	0.0
35	6.701334905913258E-4	0.0	0.0	21.53189165281724	0.0
36	6.701334905913258E-4	0.0	0.0	22.038512571704285	0.0
37	6.701334905913258E-4	0.0	0.0	22.511961882807054	0.0
38	6.701334905913258E-4	0.0	0.0	23.03148287138798	0.0
39	6.701334905913258E-4	0.0	0.0	23.55435452742186	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATACGG	35	1.2106102E-7	45.000004	2
TTCAGCG	25	3.8885446E-5	45.0	1
CGACGGT	50	2.1827873E-11	45.0	28
TATTGCG	40	6.8048394E-9	45.0	1
ACGATAG	20	7.030558E-4	45.0	1
TCTCCGT	20	7.030558E-4	45.0	45
CTCGATA	20	7.030558E-4	45.0	40
ATGCGCT	20	7.030558E-4	45.0	12
TAGTACG	20	7.030558E-4	45.0	1
CGTTTTT	3045	0.0	43.59606	1
CGGTCTA	55	6.002665E-11	40.909092	31
ACGGTCT	55	6.002665E-11	40.909092	30
CTAACGG	40	3.4544973E-7	39.375	2
CGTAAGG	80	0.0	39.375	2
TAAGCGG	40	3.4544973E-7	39.375	2
GCGTAAG	40	3.4544973E-7	39.375	1
GTACGAG	40	3.4544973E-7	39.375	1
ATTGCGG	115	0.0	39.130432	2
CACGACG	65	9.094947E-12	38.07692	26
TACGAGG	65	9.094947E-12	38.07692	2
>>END_MODULE