FastQCFastQC Report
Sat 18 Jun 2016
SRR3550355_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550355_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences768219
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31650.41199189293677974No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC11460.14917621147094773No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC10970.14279782197524404No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT9790.12743761869987594Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAAT207.031824E-445.06
CGATATA207.031824E-445.010
ATACCGG207.031824E-445.02
GATAACG302.1645592E-644.9999961
ATAACGG750.042.02
CGTTTTT18450.040.731711
TAGTCGG403.4562254E-739.3752
ATTAGCG356.245582E-638.571431
GTTAACG356.245582E-638.571431
TTACGGG2200.037.8409083
GGTCGAT301.1395695E-437.4999968
GCGTTAG301.1395695E-437.4999961
CGAATAT1200.037.49999614
CGGTCTA301.1395695E-437.49999631
GCTACGA1200.037.49999610
ACAACGA1700.037.05882313
CACAACG1700.037.05882312
CATACGA1100.036.8181818
ACATACG1100.036.8181817
TCGGGTA250.002106674736.027
Produced by FastQC (version 0.11.5)