##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3550355_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 768219 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.53508309479459 33.0 31.0 34.0 31.0 34.0 2 32.680723856087916 34.0 31.0 34.0 31.0 34.0 3 32.63909770521167 34.0 31.0 34.0 31.0 34.0 4 36.10876195459888 37.0 35.0 37.0 35.0 37.0 5 36.176583760620346 37.0 35.0 37.0 35.0 37.0 6 36.21399496758086 37.0 36.0 37.0 35.0 37.0 7 36.22550080120382 37.0 37.0 37.0 35.0 37.0 8 36.164443993184236 37.0 37.0 37.0 35.0 37.0 9 38.0282666791631 39.0 38.0 39.0 35.0 39.0 10 37.770600570930945 39.0 38.0 39.0 35.0 39.0 11 37.7057622891389 39.0 37.0 39.0 35.0 39.0 12 37.60354143805347 39.0 37.0 39.0 35.0 39.0 13 37.58020434277205 39.0 37.0 39.0 35.0 39.0 14 38.84815918377442 40.0 38.0 41.0 35.0 41.0 15 38.950403465678406 40.0 38.0 41.0 35.0 41.0 16 39.0035627861326 40.0 38.0 41.0 35.0 41.0 17 38.96822260318998 40.0 38.0 41.0 35.0 41.0 18 38.90280115435833 40.0 38.0 41.0 35.0 41.0 19 38.839576995622345 40.0 38.0 41.0 35.0 41.0 20 38.78033607604082 40.0 38.0 41.0 35.0 41.0 21 38.70192093660792 40.0 38.0 41.0 35.0 41.0 22 38.68003004351624 40.0 38.0 41.0 35.0 41.0 23 38.641352270641576 40.0 38.0 41.0 35.0 41.0 24 38.61746064598767 40.0 38.0 41.0 35.0 41.0 25 38.5389999466298 40.0 38.0 41.0 35.0 41.0 26 38.55477018922989 40.0 38.0 41.0 35.0 41.0 27 38.534848786609025 40.0 38.0 41.0 35.0 41.0 28 38.47230021647473 40.0 37.0 41.0 35.0 41.0 29 38.43864575075597 40.0 37.0 41.0 35.0 41.0 30 38.37211394146721 40.0 37.0 41.0 34.0 41.0 31 38.30802023902039 40.0 37.0 41.0 34.0 41.0 32 38.18678007182847 40.0 37.0 41.0 34.0 41.0 33 38.0867812433694 40.0 37.0 41.0 34.0 41.0 34 37.940360756503026 40.0 37.0 41.0 34.0 41.0 35 37.84617537447004 40.0 37.0 41.0 34.0 41.0 36 37.737595659571035 40.0 36.0 41.0 33.0 41.0 37 37.68431658159978 40.0 36.0 41.0 33.0 41.0 38 37.62590745607698 40.0 36.0 41.0 33.0 41.0 39 37.52865784366177 40.0 36.0 41.0 33.0 41.0 40 37.45120597121394 40.0 36.0 41.0 33.0 41.0 41 37.35686178029963 40.0 35.0 41.0 33.0 41.0 42 37.28127265792697 40.0 35.0 41.0 33.0 41.0 43 37.227497627629624 40.0 35.0 41.0 33.0 41.0 44 37.160343599937 40.0 35.0 41.0 33.0 41.0 45 37.06033565949293 39.0 35.0 41.0 33.0 41.0 46 36.9790606584841 39.0 35.0 41.0 32.0 41.0 47 36.89909648160225 39.0 35.0 41.0 32.0 41.0 48 36.82853326980978 39.0 35.0 41.0 32.0 41.0 49 36.75885001542529 39.0 35.0 41.0 32.0 41.0 50 36.67085817976384 39.0 35.0 41.0 32.0 41.0 51 35.72020348364203 38.0 35.0 40.0 29.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 12.0 9 9.0 10 8.0 11 12.0 12 5.0 13 11.0 14 17.0 15 17.0 16 33.0 17 56.0 18 98.0 19 190.0 20 314.0 21 529.0 22 823.0 23 1343.0 24 2101.0 25 3274.0 26 4691.0 27 5752.0 28 6309.0 29 6885.0 30 8083.0 31 9963.0 32 13075.0 33 18680.0 34 39026.0 35 52858.0 36 47001.0 37 71647.0 38 139406.0 39 335504.0 40 487.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.67186570496173 21.77829499140219 25.056657020979696 12.493182282656377 2 32.998142456773394 24.501346621210878 25.225358914580347 17.27515200743538 3 31.65620740960585 24.510718948633137 27.187559797401523 16.645513844359485 4 28.691558006245614 25.8776468689267 26.59358854701589 18.837206577811795 5 25.407208100815005 30.246062646198546 25.346938828641314 18.999790424345143 6 26.50194801222047 34.27525223927031 24.15691358844288 15.065886160066336 7 80.96948916910412 6.026797046154807 8.435224851246845 4.5684889334942245 8 82.29346058871234 5.287034035867377 8.200526152047788 4.218979223372502 9 75.34283843539407 8.014381315744599 10.518615134486389 6.124165114374938 10 40.04665336316858 27.73271684246289 17.56725621209577 14.653373582272764 11 30.862683687854638 25.06602934840195 25.390806527826047 18.680480435917364 12 27.72451605596842 23.42144622822398 28.858697845275895 19.995339870531712 13 25.2019280960247 23.72383395880602 30.534912570503987 20.539325374665296 14 21.161673949746103 26.107529233200427 30.766877674204885 21.96391914284859 15 21.00690037606464 25.767912535357755 32.69900900654631 20.526178082031297 16 24.55094185382033 25.781189999205957 30.250748809909673 19.41711933706404 17 24.59806383336002 25.83586190916913 28.18167736023191 21.38439689723894 18 24.762730419320533 25.534255205872284 28.9110266733835 20.791987701423682 19 24.73982028562168 27.361989224426893 27.303021664395178 20.595168825556254 20 25.915787034686723 27.18170209276261 27.297424302184663 19.605086570366005 21 25.059260445263654 27.12143282058892 28.074155937304333 19.745150796843088 22 23.97610577192181 25.364772284986444 28.312759772929336 22.346362170162415 23 23.314185147724803 26.061058109731732 28.877572671334605 21.74718407120886 24 23.136110926701893 25.31660893573317 29.597549657063936 21.949730480501003 25 22.927186127914045 27.048276598209625 27.563624435219648 22.460912838656686 26 22.44255869745476 27.406377608468418 28.055801796102415 22.095261897974407 27 22.55593782502125 26.562998311679355 29.15171324843567 21.72935061486373 28 21.119107962703342 27.85078213374051 28.623087947577446 22.4070219559787 29 22.553724914379885 26.453003635682016 28.813268091520776 22.180003358417327 30 23.575178432191862 25.58124701419777 28.833054116078877 22.010520437531486 31 23.890062599336908 26.78233680760304 27.270218518417273 22.057382074642778 32 25.048716576913616 26.227286750262618 27.694446505488667 21.029550167335096 33 24.222910394041282 26.438424459691834 27.297944987041454 22.04072015922543 34 22.925233559701073 26.505983319860615 29.07530274570142 21.493480374736894 35 24.012553711897258 26.123019607689997 28.277092860239073 21.587333820173672 36 23.294919808023494 27.83294867739538 27.47627955049276 21.395851964088365 37 23.980010908347747 27.769685467295137 27.748467559380853 20.501836064976263 38 23.949160330582817 26.978374656185277 27.032265538863264 22.040199474368638 39 24.33576883675098 25.498458121967825 27.38320713234117 22.78256590894003 40 25.262067196984194 24.716129124637636 28.45464639640519 21.567157281972978 41 22.564268782729926 25.630451733164634 28.603952779090335 23.201326705015106 42 22.611130419841217 25.858381529225387 29.28787233848681 22.242615712446582 43 22.97248571045496 25.41814248280764 29.370010374645773 22.23936143209163 44 23.2414194389881 25.383386768616763 27.879029287221485 23.496164505173656 45 22.83997141440136 24.60301033949954 27.93344085475626 24.62357739134283 46 22.723338006479924 26.038798832103865 27.67127602936142 23.566587132054792 47 22.48395314356974 25.407468443243395 29.839407773043884 22.26917064014298 48 22.233113213810125 24.812195480715786 29.77445233715906 23.180238968315024 49 22.82291898534142 24.701549948647454 29.43822009088554 23.037310975125582 50 21.4861907867418 24.77939233473788 29.572426612723717 24.1619902657966 51 21.572233959326702 24.299320896775527 28.602911409376752 25.525533734521016 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 179.0 1 459.0 2 739.0 3 3246.5 4 5754.0 5 3813.5 6 1873.0 7 1880.5 8 1888.0 9 1922.0 10 1956.0 11 1998.0 12 2040.0 13 2053.5 14 2067.0 15 2049.0 16 2031.0 17 2053.5 18 2076.0 19 2144.0 20 2212.0 21 2400.5 22 2589.0 23 2812.0 24 3035.0 25 3650.5 26 5360.0 27 6454.0 28 8401.0 29 10348.0 30 12018.5 31 13689.0 32 15387.0 33 17085.0 34 18880.5 35 20676.0 36 23017.5 37 25359.0 38 27550.5 39 29742.0 40 32425.0 41 35108.0 42 38111.0 43 41114.0 44 44532.0 45 47950.0 46 53571.5 47 59193.0 48 64322.0 49 69451.0 50 69595.5 51 69740.0 52 62152.5 53 54565.0 54 50029.5 55 45494.0 56 42294.0 57 39094.0 58 37293.0 59 35492.0 60 34131.5 61 32771.0 62 30027.5 63 27284.0 64 23419.5 65 19555.0 66 16293.0 67 13031.0 68 10897.5 69 8764.0 70 7042.5 71 5321.0 72 4531.0 73 3741.0 74 2852.5 75 1642.0 76 1320.0 77 1019.5 78 719.0 79 478.5 80 238.0 81 174.0 82 110.0 83 107.0 84 104.0 85 64.5 86 25.0 87 16.0 88 7.0 89 4.5 90 2.0 91 2.0 92 2.0 93 1.0 94 0.0 95 1.0 96 2.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 768219.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.45164645989736 #Duplication Level Percentage of deduplicated Percentage of total 1 69.30960838096992 24.57139732596085 2 8.809611415221205 6.2463045868299645 3 3.8149447662144143 4.057382193476077 4 2.5860660960924995 3.6672120384239304 5 2.0656396711208633 3.6615163667057757 6 1.7686844163005464 3.762166477549014 7 1.522700030603461 3.7787556204600157 8 1.3831818450191424 3.9228859007493755 9 1.2860638292819453 4.103377218050879 >10 7.391259672526316 38.76831330704038 >50 0.038301467070563366 0.9491893563164224 >100 0.02172871023334827 1.5156996753145733 >500 0.0011048496728819199 0.28971811349149096 >1k 0.0011048496728819199 0.7060818196313579 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3165 0.41199189293677974 No Hit CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC 1146 0.14917621147094773 No Hit GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC 1097 0.14279782197524404 No Hit CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT 979 0.12743761869987594 Illumina Single End Adapter 1 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 6.508560709901734E-4 0.0 0.0 0.1329048096961934 0.0 2 6.508560709901734E-4 0.0 0.0 0.5383881419230714 0.0 3 6.508560709901734E-4 0.0 0.0 0.8321845723680357 0.0 4 6.508560709901734E-4 0.0 0.0 1.1386076105902092 0.0 5 6.508560709901734E-4 0.0 0.0 2.053320732759799 0.0 6 6.508560709901734E-4 0.0 0.0 2.9112792055390453 0.0 7 6.508560709901734E-4 0.0 0.0 3.6093874272831057 0.0 8 6.508560709901734E-4 0.0 0.0 4.691110217268774 0.0 9 6.508560709901734E-4 0.0 0.0 5.281827187299455 0.0 10 6.508560709901734E-4 0.0 0.0 6.226740031162989 0.0 11 6.508560709901734E-4 0.0 0.0 7.309764533290637 0.0 12 6.508560709901734E-4 0.0 0.0 8.157829993790832 0.0 13 6.508560709901734E-4 0.0 0.0 8.560189216876958 0.0 14 6.508560709901734E-4 0.0 0.0 8.775362233946309 0.0 15 6.508560709901734E-4 0.0 0.0 9.052496748973926 0.0 16 6.508560709901734E-4 0.0 0.0 9.546366335641268 0.0 17 6.508560709901734E-4 0.0 0.0 10.096209544413767 0.0 18 6.508560709901734E-4 0.0 0.0 10.681198981019735 0.0 19 6.508560709901734E-4 0.0 0.0 11.07158245239964 0.0 20 6.508560709901734E-4 0.0 0.0 11.465480546562894 0.0 21 6.508560709901734E-4 0.0 0.0 11.96520783786915 1.301712141980347E-4 22 6.508560709901734E-4 0.0 0.0 12.500732213079864 1.301712141980347E-4 23 6.508560709901734E-4 0.0 0.0 13.006187037810832 1.301712141980347E-4 24 6.508560709901734E-4 0.0 0.0 13.435752044664348 1.301712141980347E-4 25 6.508560709901734E-4 0.0 0.0 13.822751064475105 1.301712141980347E-4 26 6.508560709901734E-4 0.0 0.0 14.18007104744871 1.301712141980347E-4 27 6.508560709901734E-4 0.0 0.0 14.559389965621783 1.301712141980347E-4 28 6.508560709901734E-4 0.0 0.0 14.929336556372597 1.301712141980347E-4 29 6.508560709901734E-4 0.0 0.0 15.33716297045504 1.301712141980347E-4 30 6.508560709901734E-4 0.0 0.0 15.7836502351543 1.301712141980347E-4 31 6.508560709901734E-4 0.0 0.0 16.194730929591692 1.301712141980347E-4 32 6.508560709901734E-4 0.0 0.0 16.602297001245738 1.301712141980347E-4 33 6.508560709901734E-4 0.0 0.0 16.992159787768852 1.301712141980347E-4 34 6.508560709901734E-4 0.0 0.0 17.393347469927196 1.301712141980347E-4 35 6.508560709901734E-4 0.0 0.0 17.803516965865203 1.301712141980347E-4 36 7.81027285188208E-4 0.0 0.0 18.206657216236515 1.301712141980347E-4 37 7.81027285188208E-4 0.0 0.0 18.616696540960326 1.301712141980347E-4 38 7.81027285188208E-4 0.0 0.0 19.045090006886056 1.301712141980347E-4 39 7.81027285188208E-4 0.0 0.0 19.57410582138687 1.301712141980347E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTAAT 20 7.031824E-4 45.0 6 CGATATA 20 7.031824E-4 45.0 10 ATACCGG 20 7.031824E-4 45.0 2 GATAACG 30 2.1645592E-6 44.999996 1 ATAACGG 75 0.0 42.0 2 CGTTTTT 1845 0.0 40.73171 1 TAGTCGG 40 3.4562254E-7 39.375 2 ATTAGCG 35 6.245582E-6 38.57143 1 GTTAACG 35 6.245582E-6 38.57143 1 TTACGGG 220 0.0 37.840908 3 GGTCGAT 30 1.1395695E-4 37.499996 8 GCGTTAG 30 1.1395695E-4 37.499996 1 CGAATAT 120 0.0 37.499996 14 CGGTCTA 30 1.1395695E-4 37.499996 31 GCTACGA 120 0.0 37.499996 10 ACAACGA 170 0.0 37.058823 13 CACAACG 170 0.0 37.058823 12 CATACGA 110 0.0 36.81818 18 ACATACG 110 0.0 36.81818 17 TCGGGTA 25 0.0021066747 36.0 27 >>END_MODULE