SRR3550354_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550354_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	684620
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	2072	0.3026496450585726	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1958	0.2859980719231106	No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	1707	0.24933539773889166	No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT	1534	0.22406590517367297	TruSeq Adapter, Index 27 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	859	0.12547106424001636	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	767	0.11203295258683649	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGT	35	1.210974E-7	45.000004	45
ATATGCG	35	1.210974E-7	45.000004	1
TAATACG	20	7.031285E-4	45.0	31
TAGGTCG	20	7.031285E-4	45.0	1
GCGTAAG	20	7.031285E-4	45.0	1
TGCGTCG	20	7.031285E-4	45.0	1
ACACGAC	115	0.0	41.08696	26
GTACGAG	45	1.9263098E-8	40.0	1
CTCACGA	40	3.4554978E-7	39.375	24
TTACGCG	40	3.4554978E-7	39.375	1
CGGTCTA	40	3.4554978E-7	39.375	31
ACGGTCT	40	3.4554978E-7	39.375	30
TAAGGGA	580	0.0	39.181038	4
CGTTTTT	1155	0.0	38.57143	1
TGTAGCG	65	9.094947E-12	38.076927	1
ACCGGGT	30	1.1394395E-4	37.500004	4
GCTTACG	30	1.1394395E-4	37.500004	1
GCATACG	30	1.1394395E-4	37.500004	1
TATGCGG	90	0.0	37.5	2
ATTGCGG	90	0.0	37.5	2