##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550354_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	684620
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36674505565131	33.0	31.0	34.0	30.0	34.0
2	32.51186059419824	34.0	31.0	34.0	31.0	34.0
3	32.48860243638807	34.0	31.0	34.0	30.0	34.0
4	35.97988080979229	37.0	35.0	37.0	35.0	37.0
5	36.05561771493675	37.0	35.0	37.0	35.0	37.0
6	36.10148403493909	37.0	35.0	37.0	35.0	37.0
7	36.15978060822062	37.0	37.0	37.0	35.0	37.0
8	36.09543834535947	37.0	37.0	37.0	35.0	37.0
9	37.94098331921358	39.0	38.0	39.0	35.0	39.0
10	37.672372995238234	39.0	37.0	39.0	35.0	39.0
11	37.592300838421316	39.0	37.0	39.0	35.0	39.0
12	37.51999211241272	39.0	37.0	39.0	35.0	39.0
13	37.49255499401128	39.0	37.0	39.0	35.0	39.0
14	38.73237562443399	40.0	38.0	41.0	35.0	41.0
15	38.82894306330519	40.0	38.0	41.0	35.0	41.0
16	38.88583301685607	40.0	38.0	41.0	35.0	41.0
17	38.85874499722474	40.0	38.0	41.0	35.0	41.0
18	38.81276036341328	40.0	38.0	41.0	35.0	41.0
19	38.765009786450875	40.0	38.0	41.0	35.0	41.0
20	38.7102684701002	40.0	38.0	41.0	35.0	41.0
21	38.63070754579183	40.0	38.0	41.0	35.0	41.0
22	38.60389705237942	40.0	38.0	41.0	35.0	41.0
23	38.57472612544185	40.0	38.0	41.0	35.0	41.0
24	38.53574829832608	40.0	38.0	41.0	35.0	41.0
25	38.467492915778095	40.0	38.0	41.0	34.0	41.0
26	38.48336595483626	40.0	38.0	41.0	35.0	41.0
27	38.4630524962753	40.0	38.0	41.0	35.0	41.0
28	38.393941164441586	40.0	37.0	41.0	34.0	41.0
29	38.34994303409191	40.0	37.0	41.0	34.0	41.0
30	38.24061669247174	40.0	37.0	41.0	34.0	41.0
31	38.170447839677486	40.0	37.0	41.0	34.0	41.0
32	38.07882182816745	40.0	37.0	41.0	34.0	41.0
33	37.96824077590488	40.0	37.0	41.0	34.0	41.0
34	37.859656451754255	40.0	37.0	41.0	33.0	41.0
35	37.79433700446963	40.0	37.0	41.0	33.0	41.0
36	37.67380006426923	40.0	36.0	41.0	33.0	41.0
37	37.62115772253221	40.0	36.0	41.0	33.0	41.0
38	37.536747392714204	40.0	36.0	41.0	33.0	41.0
39	37.46235283807076	40.0	36.0	41.0	33.0	41.0
40	37.37665858432415	40.0	36.0	41.0	33.0	41.0
41	37.34191668370775	40.0	35.0	41.0	33.0	41.0
42	37.31127194648126	40.0	35.0	41.0	33.0	41.0
43	37.2546872717712	40.0	35.0	41.0	33.0	41.0
44	37.15143875434548	39.0	35.0	41.0	32.0	41.0
45	37.02637229411936	39.0	35.0	41.0	32.0	41.0
46	36.91365575063539	39.0	35.0	41.0	32.0	41.0
47	36.82283310449593	39.0	35.0	41.0	31.0	41.0
48	36.76277204872776	39.0	35.0	41.0	31.0	41.0
49	36.7022216704157	39.0	35.0	41.0	31.0	41.0
50	36.58110338581987	39.0	35.0	41.0	31.0	41.0
51	35.56895358008823	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	2.0
9	8.0
10	9.0
11	5.0
12	9.0
13	9.0
14	12.0
15	18.0
16	25.0
17	56.0
18	96.0
19	182.0
20	293.0
21	504.0
22	830.0
23	1220.0
24	1946.0
25	2938.0
26	3814.0
27	4790.0
28	5608.0
29	6431.0
30	7767.0
31	10000.0
32	12940.0
33	17841.0
34	34251.0
35	48297.0
36	43990.0
37	68242.0
38	132434.0
39	279668.0
40	382.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.120212672723554	21.996874178376324	26.484327071952325	11.398586076947796
2	31.632292366568315	25.01811223744559	25.72405129853057	17.625544097455524
3	31.958020507726914	24.62621600303818	27.031053723233327	16.384709766001578
4	28.31833717974935	25.931319564137773	26.727673746019693	19.02266951009319
5	24.9031579562385	30.288335134819317	25.29943618357629	19.509070725365895
6	25.57141187812217	34.52411556776022	24.30662265198212	15.597849902135492
7	80.84762349916743	5.558850165055067	8.640851859425666	4.952674476351845
8	81.81414507317928	4.93894423183664	8.124068826502292	5.122841868481785
9	75.1652011334755	7.710408693873974	10.699220005258391	6.42517016739213
10	38.902164704507605	30.124740732084952	16.798223832198882	14.174870731208552
11	28.48222371534574	24.51243025327919	27.391107475679938	19.61423855569513
12	26.296923840962872	22.512488679851597	30.42768251000555	20.76290496917998
13	23.86959189039175	24.194735765826298	32.07195232391692	19.863720019865035
14	20.619759866787415	26.992346119014925	30.341065116414946	22.04682889778271
15	19.767900441120624	25.805556367035727	33.896760246560135	20.529782945283515
16	22.28871490753995	26.45365312143963	30.48494055096258	20.772691420057843
17	22.434197072828724	26.319418071338845	28.411527562735532	22.8348572930969
18	22.928924074669162	25.715287312669805	29.93018024597587	21.425608366685168
19	23.415325289941865	27.629926090385908	27.31442259939821	21.64032602027402
20	24.928427448803717	27.300400222020976	27.7073412988227	20.063831030352606
21	23.989366363822267	27.74794776664427	28.470392334433704	19.792293535099763
22	22.97128333966288	25.363705413221933	27.75306009172972	23.91195115538547
23	22.023458268820658	26.995267447635186	28.165551692909936	22.81572259063422
24	22.179895416435393	25.56352429084748	29.233881569337733	23.022698723379392
25	21.889807484443924	27.586252227513075	26.970436154363004	23.553504133679997
26	21.974087815138326	27.784318307966466	27.54535362682948	22.69624025006573
27	23.630919342116794	26.658000058426573	27.30726534427858	22.403815255178056
28	20.957319388858053	27.67184715608659	28.295842949373373	23.074990505681985
29	24.18275831848325	25.406941076801733	27.211591831965176	23.198708772749846
30	25.4510531389676	25.29373959276679	27.478747334287632	21.776459933977975
31	25.274312757442086	27.365107650959658	25.540445794747452	21.820133796850808
32	26.527708801963136	27.17098536414361	25.02337062896205	21.277935204931204
33	24.501183138091204	26.874470509187574	26.07344220151325	22.55090415120797
34	22.737284917180332	26.351406619730653	28.66685168414595	22.244456778943064
35	24.27872396365867	26.267710554760303	27.1264351026847	22.327130378896324
36	24.208027811048467	27.920890420963456	26.669101107183547	21.201980660804534
37	23.65078437673454	28.04694575092752	27.567993923636468	20.73427594870147
38	22.285793578919694	28.268382460342966	26.892874879495192	22.55294908124215
39	23.199146972042886	24.758406123104788	28.510414536531215	23.53203236832111
40	23.30548333382022	24.89132657532646	30.213549122140748	21.58964096871257
41	21.17641903537729	26.481990009056116	27.56565686074027	24.775934094826326
42	22.974642867576172	25.88998276416114	27.525050392918697	23.610323975343984
43	23.55218953580088	25.075662411264645	27.802576611843065	23.56957144109141
44	23.14933831906751	25.375682860564986	27.30741141070959	24.167567409657913
45	23.152551780549793	23.75025561625427	27.898250124156466	25.198942479039466
46	23.683941456574452	25.14066197306535	27.80126201396395	23.374134556396246
47	21.834740439952093	25.249043264876864	29.743653413572492	23.17256288159855
48	21.647191142531625	24.738248955625018	29.103590312874296	24.510969588969065
49	23.171832549443486	23.603020653793344	29.510239256813996	23.714907539949166
50	21.567731004060647	23.61149250679209	29.635126055329962	25.1856504338173
51	21.31196868335719	23.290000292132863	27.90569951213812	27.492331512371827
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	173.0
1	368.0
2	563.0
3	2040.0
4	3517.0
5	2406.0
6	1295.0
7	1287.0
8	1279.0
9	1304.5
10	1330.0
11	1390.5
12	1451.0
13	1498.5
14	1546.0
15	1496.5
16	1447.0
17	1452.5
18	1458.0
19	1460.0
20	1462.0
21	1670.5
22	1879.0
23	2237.5
24	2596.0
25	3171.5
26	4354.0
27	4961.0
28	6412.0
29	7863.0
30	9497.0
31	11131.0
32	12281.5
33	13432.0
34	15487.0
35	17542.0
36	18771.0
37	20000.0
38	22451.5
39	24903.0
40	27736.0
41	30569.0
42	33108.0
43	35647.0
44	41092.5
45	46538.0
46	54562.5
47	62587.0
48	68659.5
49	74732.0
50	72720.0
51	70708.0
52	60874.5
53	51041.0
54	45197.5
55	39354.0
56	36317.0
57	33280.0
58	31100.5
59	28921.0
60	27555.0
61	26189.0
62	23079.5
63	19970.0
64	17321.5
65	14673.0
66	12009.5
67	9346.0
68	7994.0
69	6642.0
70	5537.0
71	4432.0
72	3552.0
73	2672.0
74	2219.5
75	1391.0
76	1015.0
77	795.5
78	576.0
79	400.5
80	225.0
81	148.0
82	71.0
83	51.0
84	31.0
85	35.5
86	40.0
87	24.0
88	8.0
89	5.5
90	3.0
91	4.5
92	6.0
93	4.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	684620.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.22843760423086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.34577613036883	26.560917749948597
2	9.56435612572247	7.121320705022045
3	3.8022471328115057	4.246551604192166
4	2.3224514931823115	3.4584496201116197
5	1.7593507745580537	3.2748940267294873
6	1.41914332492987	3.169949323416743
7	1.275830704403252	3.324802864070686
8	1.0573352477344409	3.1490351437628488
9	0.9672008748649328	3.240663967859999
>10	6.398118641675074	37.258255395786165
>50	0.0519688423112658	1.3371545111151903
>100	0.032677377362563695	2.295133412079205
>500	0.0019685167085881725	0.49716409309487575
>1k	0.0015748133668705378	1.0657075828103895
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	2072	0.3026496450585726	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1958	0.2859980719231106	No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	1707	0.24933539773889166	No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT	1534	0.22406590517367297	TruSeq Adapter, Index 27 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	859	0.12547106424001636	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	767	0.11203295258683649	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.921328620256493E-4	0.0	0.0	0.23180742601735269	0.0
2	2.921328620256493E-4	0.0	0.0	1.022903216382811	0.0
3	2.921328620256493E-4	0.0	0.0	1.4650463030586311	0.0
4	2.921328620256493E-4	0.0	0.0	2.0276941953200316	0.0
5	2.921328620256493E-4	0.0	0.0	3.5678186439192543	0.0
6	2.921328620256493E-4	0.0	0.0	4.6919458969939525	0.0
7	2.921328620256493E-4	0.0	0.0	5.762320703455932	0.0
8	2.921328620256493E-4	0.0	0.0	7.471590079168005	0.0
9	2.921328620256493E-4	0.0	0.0	8.268528526773977	0.0
10	2.921328620256493E-4	0.0	0.0	9.711372732318658	0.0
11	2.921328620256493E-4	0.0	0.0	11.432181356080745	0.0
12	2.921328620256493E-4	0.0	0.0	12.825362975081067	0.0
13	2.921328620256493E-4	0.0	0.0	13.405977038357046	0.0
14	2.921328620256493E-4	0.0	0.0	13.677076334316848	0.0
15	2.921328620256493E-4	0.0	0.0	14.070433233034384	0.0
16	2.921328620256493E-4	0.0	0.0	14.755776927346558	0.0
17	2.921328620256493E-4	0.0	0.0	15.616108206012095	0.0
18	2.921328620256493E-4	0.0	0.0	16.517045952499195	0.0
19	4.381992930384739E-4	0.0	0.0	17.104086938739737	0.0
20	4.381992930384739E-4	0.0	0.0	17.657678712278344	0.0
21	4.381992930384739E-4	0.0	0.0	18.345359469486723	0.0
22	4.381992930384739E-4	0.0	0.0	19.096871257047706	0.0
23	4.381992930384739E-4	0.0	0.0	19.825888814232712	0.0
24	5.842657240512986E-4	0.0	0.0	20.413367999766294	0.0
25	5.842657240512986E-4	0.0	0.0	20.918173585346615	0.0
26	5.842657240512986E-4	0.0	0.0	21.390698489673103	0.0
27	5.842657240512986E-4	0.0	0.0	21.877830037100875	0.0
28	5.842657240512986E-4	0.0	0.0	22.371534573924222	0.0
29	5.842657240512986E-4	0.0	0.0	22.862902047851364	0.0
30	7.303321550641232E-4	0.0	0.0	23.46878560369256	0.0
31	7.303321550641232E-4	0.0	0.0	23.994478688907716	0.0
32	7.303321550641232E-4	0.0	0.0	24.50863252607286	0.0
33	7.303321550641232E-4	0.0	0.0	25.000292132862025	0.0
34	7.303321550641232E-4	0.0	0.0	25.474423767929654	0.0
35	8.763985860769478E-4	0.0	0.0	25.989892202973913	0.0
36	8.763985860769478E-4	0.0	0.0	26.485057404107387	0.0
37	8.763985860769478E-4	0.0	0.0	26.989570856825683	0.0
38	8.763985860769478E-4	0.0	0.0	27.49408430954398	0.0
39	8.763985860769478E-4	0.0	0.0	28.08726008588706	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGT	35	1.210974E-7	45.000004	45
ATATGCG	35	1.210974E-7	45.000004	1
TAATACG	20	7.031285E-4	45.0	31
TAGGTCG	20	7.031285E-4	45.0	1
GCGTAAG	20	7.031285E-4	45.0	1
TGCGTCG	20	7.031285E-4	45.0	1
ACACGAC	115	0.0	41.08696	26
GTACGAG	45	1.9263098E-8	40.0	1
CTCACGA	40	3.4554978E-7	39.375	24
TTACGCG	40	3.4554978E-7	39.375	1
CGGTCTA	40	3.4554978E-7	39.375	31
ACGGTCT	40	3.4554978E-7	39.375	30
TAAGGGA	580	0.0	39.181038	4
CGTTTTT	1155	0.0	38.57143	1
TGTAGCG	65	9.094947E-12	38.076927	1
ACCGGGT	30	1.1394395E-4	37.500004	4
GCTTACG	30	1.1394395E-4	37.500004	1
GCATACG	30	1.1394395E-4	37.500004	1
TATGCGG	90	0.0	37.5	2
ATTGCGG	90	0.0	37.5	2
>>END_MODULE