FastQCFastQC Report
Sat 18 Jun 2016
SRR3550352_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550352_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472753
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10340.21871886587710707No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC7820.16541407458017188Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC7280.1539916193022572Illumina Single End Adapter 1 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6010.1271276967041986No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGCT5290.11189775633364568Illumina Single End Adapter 1 (95% over 22bp)
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC4930.10428278614836924No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC351.2099008E-745.025
ATAGCCG207.029069E-445.01
TGCGACG253.88731E-545.01
GCGATAT207.029069E-445.09
GCGATAC253.88731E-545.09
CCGTTAC207.029069E-445.044
ATACCGG207.029069E-445.02
TATAGCG302.1627802E-644.9999961
TCGATCA850.042.35294317
TATGGGC1350.040.0000044
CGAAAGG900.040.02
TACGGGA1300.039.8076934
TACGGGT403.45246E-739.3754
CGGTCTA403.45246E-739.37531
ATAACGC403.45246E-739.37511
CGTATGG750.039.0000042
TAGGGAC2600.038.942315
AGGTACG356.240489E-638.5714261
CGCATCG356.240489E-638.57142621
ATGGTCG356.240489E-638.57142644
Produced by FastQC (version 0.11.5)