##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550352_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	472753
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.296949992913845	33.0	31.0	34.0	30.0	34.0
2	32.40539985996916	34.0	31.0	34.0	30.0	34.0
3	32.50598938557767	34.0	31.0	34.0	31.0	34.0
4	35.9980327993688	37.0	35.0	37.0	35.0	37.0
5	35.946614828462224	37.0	35.0	37.0	35.0	37.0
6	35.96482306828302	37.0	35.0	37.0	35.0	37.0
7	36.10443720082157	37.0	35.0	37.0	35.0	37.0
8	36.07014233648438	37.0	35.0	37.0	35.0	37.0
9	37.619837420386546	39.0	38.0	39.0	35.0	39.0
10	37.406241737228534	39.0	37.0	39.0	35.0	39.0
11	37.34153987388763	39.0	37.0	39.0	34.0	39.0
12	37.18907336389193	39.0	37.0	39.0	34.0	39.0
13	37.1194725363985	39.0	37.0	39.0	33.0	39.0
14	38.31721638995416	40.0	38.0	41.0	34.0	41.0
15	38.404989497686955	40.0	38.0	41.0	34.0	41.0
16	38.42985660588087	40.0	38.0	41.0	34.0	41.0
17	38.43498401913896	40.0	38.0	41.0	34.0	41.0
18	38.407396674373295	40.0	37.0	41.0	34.0	41.0
19	38.358290693025744	40.0	37.0	41.0	34.0	41.0
20	38.24405556389912	40.0	37.0	41.0	34.0	41.0
21	38.180813236510396	40.0	37.0	41.0	34.0	41.0
22	38.14219264605407	40.0	37.0	41.0	34.0	41.0
23	38.10568944036315	40.0	37.0	41.0	34.0	41.0
24	38.07454844284436	40.0	36.0	41.0	34.0	41.0
25	38.01927010510774	40.0	36.0	41.0	34.0	41.0
26	37.873743794328114	40.0	36.0	41.0	33.0	41.0
27	37.81831950299628	40.0	36.0	41.0	33.0	41.0
28	37.73938822175639	40.0	36.0	41.0	33.0	41.0
29	37.673197208690375	40.0	36.0	41.0	33.0	41.0
30	37.64197160039175	40.0	36.0	41.0	33.0	41.0
31	37.53970466607298	40.0	36.0	41.0	33.0	41.0
32	37.379733179905784	40.0	35.0	41.0	33.0	41.0
33	37.2926813790711	40.0	35.0	41.0	32.0	41.0
34	37.12079246456395	39.0	35.0	41.0	32.0	41.0
35	37.092729184161705	39.0	35.0	41.0	32.0	41.0
36	36.94591890479806	39.0	35.0	41.0	31.0	41.0
37	36.89088382305348	39.0	35.0	41.0	31.0	41.0
38	36.78065501435211	39.0	35.0	41.0	31.0	41.0
39	36.696640740513544	39.0	35.0	41.0	31.0	41.0
40	36.61936571528896	39.0	35.0	41.0	31.0	41.0
41	36.495578029118796	39.0	35.0	41.0	31.0	41.0
42	36.42194126742718	39.0	35.0	41.0	30.0	41.0
43	36.357569386127636	39.0	35.0	41.0	30.0	41.0
44	36.16157486044509	38.0	35.0	41.0	30.0	41.0
45	36.11477875338708	38.0	35.0	40.0	30.0	41.0
46	36.00373979646877	38.0	35.0	40.0	30.0	41.0
47	35.90198052682902	38.0	35.0	40.0	30.0	41.0
48	35.76956888692404	38.0	35.0	40.0	29.0	41.0
49	35.69262384374081	38.0	35.0	40.0	29.0	41.0
50	35.599924273352045	38.0	35.0	40.0	29.0	41.0
51	34.39882560237587	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	2.0
11	6.0
12	8.0
13	9.0
14	18.0
15	19.0
16	32.0
17	79.0
18	143.0
19	237.0
20	402.0
21	629.0
22	936.0
23	1388.0
24	1924.0
25	2594.0
26	3637.0
27	4346.0
28	5133.0
29	5859.0
30	7321.0
31	9063.0
32	11870.0
33	16190.0
34	29479.0
35	39516.0
36	36007.0
37	52352.0
38	88705.0
39	154726.0
40	120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.06498319418385	21.39637400503011	23.972560724099054	11.56608207668698
2	33.25669006859819	24.29577390307412	24.373615820523614	18.073920207804075
3	32.5080961939956	24.214759081380762	25.74938710066356	17.52775762396008
4	29.91710258845528	25.697351471064174	25.070385592476406	19.315160348004138
5	25.900628869621134	31.220743178784694	22.925713850573132	19.95291410102104
6	28.20754178186072	35.54456555537458	21.590978798653843	14.656913864110857
7	81.7101107766635	6.170875700418612	7.387367187516526	4.731646335401362
8	81.79387544870154	5.726457579327894	7.679062851002532	4.800604120968032
9	74.87504045452911	8.098097738142327	10.276613791980168	6.7502480153483955
10	41.98915712856397	25.21697376854298	16.843890995932338	15.949978106960716
11	31.56912806476109	24.751826006392346	23.28023301808767	20.398812910758895
12	29.099127874386838	22.245231653738845	27.510772009907924	21.144868461966396
13	24.933950709990206	24.042999198312863	29.245716050453407	21.777334041243524
14	21.219960529071205	26.76852394379306	28.812297330741423	23.199218196394312
15	21.394681789433385	25.44605745495005	32.264205621117156	20.89505513449941
16	24.11957195406481	23.27367568265035	30.478918166569013	22.12783419671583
17	23.50191326125905	23.956907729829318	27.73329836087767	24.80788064803396
18	23.84606760824363	24.192125697774525	28.39008953935776	23.571717154624086
19	24.95129591985667	25.86086180309802	26.059485608763982	23.12835666828132
20	27.291630090131637	24.947276907814437	26.023314500383922	21.737778501670004
21	26.377410613999274	25.62056718836263	25.869111354132073	22.132910843506018
22	24.603968668628227	24.133109678838633	25.717869585174498	25.545052067358643
23	24.167377044672374	25.73034967520037	26.034948482611426	24.067324797515827
24	24.231046656499274	24.17203063756338	27.749374409046585	23.847548296890768
25	23.753418804322767	25.37138844174442	26.091637705101817	24.783555048830998
26	23.238139155119057	26.394332769966557	26.347162260207764	24.020365814706622
27	23.44564709266784	25.94103051699302	26.331509265938024	24.281813124401115
28	22.100970274117774	26.840231579704408	26.554881724706135	24.50391642147168
29	23.337768348376425	25.133209096504938	26.530556125503168	24.998466429615466
30	23.773090810634727	25.2707016137391	26.044890249242204	24.911317326383966
31	24.031999796934127	27.061911822875796	24.079170306692923	24.826918073497154
32	24.74844157519889	27.050700894547468	24.352674652514104	23.84818287773954
33	24.219412674271766	27.225422154909644	23.92348647179394	24.63167869902465
34	22.73047447610063	26.50115387951002	26.202054772788326	24.56631687160103
35	23.735015959708345	25.922204618479416	24.605872411174545	25.736907010637687
36	22.915983610891946	28.40193504853486	23.988848299217562	24.693233041355633
37	23.408418349539822	28.56502232666953	25.6472195840111	22.379339739779546
38	23.276848586894214	28.282633848965528	24.57329726093753	23.86722030320273
39	23.519893051974286	26.399409416756743	25.93341554680774	24.14728198446123
40	24.350347856068602	24.430093516064414	27.827639380395258	23.391919247471723
41	22.26955725294181	25.27810505697478	26.691739661091525	25.760598028991883
42	23.220159364403823	25.157534695707906	26.0002580628785	25.62204787700977
43	22.961884958953195	25.08286568250228	26.419716003917483	25.535533354627045
44	22.32307357118834	25.983335906911222	26.023314500383922	25.670276021516518
45	22.794355614877112	24.904548463997056	26.35139279919958	25.949703121926248
46	23.35532508519248	26.108136807169917	25.80776853875068	24.728769568886925
47	22.917675826488672	25.51734203696222	27.36756826503481	24.1974138715143
48	22.867755466385194	24.59021941690481	26.91003547306945	25.631989643640544
49	22.11493105279078	24.91343259587988	27.607016771971832	25.364619579357505
50	21.129215467696664	25.00735056149829	27.617593119451385	26.24584085135367
51	21.489657389799746	24.662773160614528	26.647107474727814	27.20046197485791
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	177.0
1	434.5
2	692.0
3	1367.0
4	2042.0
5	1446.5
6	851.0
7	853.5
8	856.0
9	901.5
10	947.0
11	972.5
12	998.0
13	1042.0
14	1086.0
15	1015.5
16	945.0
17	978.5
18	1012.0
19	1025.0
20	1038.0
21	1085.0
22	1132.0
23	1331.5
24	1531.0
25	1697.0
26	2561.0
27	3259.0
28	3468.5
29	3678.0
30	4886.0
31	6094.0
32	6785.0
33	7476.0
34	8762.0
35	10048.0
36	10938.0
37	11828.0
38	12927.0
39	14026.0
40	16605.0
41	19184.0
42	21448.0
43	23712.0
44	26192.5
45	28673.0
46	32586.5
47	36500.0
48	39313.5
49	42127.0
50	41258.5
51	40390.0
52	37003.5
53	33617.0
54	30872.5
55	28128.0
56	26854.0
57	25580.0
58	24921.0
59	24262.0
60	23945.0
61	23628.0
62	22562.0
63	21496.0
64	18628.0
65	15760.0
66	13887.5
67	12015.0
68	10206.5
69	8398.0
70	7427.5
71	6457.0
72	5224.5
73	3992.0
74	3598.5
75	2519.5
76	1834.0
77	1452.0
78	1070.0
79	840.5
80	611.0
81	433.5
82	256.0
83	201.5
84	147.0
85	109.5
86	72.0
87	53.5
88	35.0
89	25.0
90	15.0
91	11.0
92	7.0
93	5.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	472753.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.18951616267567
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.71376031751916	23.40620762989708
2	8.41150022905906	5.415242451512935
3	3.0277057883814087	2.9238115323279015
4	1.7181771366644654	2.2122916284400245
5	1.2408246449451696	1.9970772481754422
6	1.0817479148863813	2.089256519410553
7	0.9979361611974787	2.2486157533129334
8	0.8819800950854053	2.2712410020727902
9	0.7909633067504884	2.2914653532055422
>10	9.039890969699867	51.486522724827644
>50	0.06653004839261308	1.505036073557523
>100	0.02568982066645456	1.3742094664308686
>500	0.0026348534016876466	0.5597767306553763
>1k	6.587133504219117E-4	0.2192458861733557
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1034	0.21871886587710707	No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC	782	0.16541407458017188	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC	728	0.1539916193022572	Illumina Single End Adapter 1 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	601	0.1271276967041986	No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGCT	529	0.11189775633364568	Illumina Single End Adapter 1 (95% over 22bp)
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC	493	0.10428278614836924	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1152694959101264E-4	0.0	0.0	0.12818533145215366	0.0
2	2.1152694959101264E-4	0.0	0.0	0.5948137822499275	0.0
3	2.1152694959101264E-4	0.0	0.0	0.8920091464253003	0.0
4	2.1152694959101264E-4	0.0	0.0	1.222625768636053	0.0
5	2.1152694959101264E-4	0.0	0.0	2.192053778611664	0.0
6	2.1152694959101264E-4	0.0	0.0	2.922879389448613	0.0
7	2.1152694959101264E-4	0.0	0.0	3.584324160819709	0.0
8	2.1152694959101264E-4	0.0	0.0	4.689129418533568	0.0
9	2.1152694959101264E-4	0.0	0.0	5.162103677819073	0.0
10	2.1152694959101264E-4	0.0	0.0	6.000596505997847	0.0
11	2.1152694959101264E-4	0.0	0.0	7.074518829071418	0.0
12	2.1152694959101264E-4	0.0	0.0	7.883609411257042	0.0
13	2.1152694959101264E-4	0.0	0.0	8.231359716384667	0.0
14	2.1152694959101264E-4	0.0	0.0	8.391485617225063	0.0
15	4.230538991820253E-4	0.0	0.0	8.655048196415464	0.0
16	4.230538991820253E-4	0.0	0.0	9.198249402965185	0.0
17	4.230538991820253E-4	0.0	0.0	9.82584986240172	0.0
18	4.230538991820253E-4	0.0	0.0	10.47544912459572	0.0
19	4.230538991820253E-4	0.0	0.0	10.891734161390833	0.0
20	4.230538991820253E-4	0.0	0.0	11.270155874209154	0.0
21	4.230538991820253E-4	0.0	0.0	11.763013666756214	0.0
22	4.230538991820253E-4	0.0	0.0	12.283581489699696	0.0
23	4.230538991820253E-4	0.0	0.0	12.78595799497835	0.0
24	4.230538991820253E-4	0.0	0.0	13.249836595431441	0.0
25	4.230538991820253E-4	0.0	0.0	13.631431212493627	0.0
26	4.230538991820253E-4	0.0	0.0	13.966489900645792	0.0
27	4.230538991820253E-4	0.0	0.0	14.299010265402863	0.0
28	4.230538991820253E-4	0.0	0.0	14.643587666286622	0.0
29	4.230538991820253E-4	0.0	0.0	15.04400818186241	0.0
30	6.345808487730379E-4	0.0	0.0	15.486522560406808	0.0
31	6.345808487730379E-4	0.0	0.0	15.874462985956725	0.0
32	6.345808487730379E-4	0.0	0.0	16.272556705087013	0.0
33	6.345808487730379E-4	0.0	0.0	16.65436284909879	0.0
34	6.345808487730379E-4	0.0	0.0	17.035322885312205	0.0
35	6.345808487730379E-4	0.0	0.0	17.4378586703839	0.0
36	6.345808487730379E-4	0.0	0.0	17.83743307816132	0.0
37	6.345808487730379E-4	0.0	0.0	18.216700898778008	0.0
38	6.345808487730379E-4	0.0	0.0	18.600410785336106	0.0
39	8.461077983640506E-4	0.0	0.0	18.977563336456882	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	35	1.2099008E-7	45.0	25
ATAGCCG	20	7.029069E-4	45.0	1
TGCGACG	25	3.88731E-5	45.0	1
GCGATAT	20	7.029069E-4	45.0	9
GCGATAC	25	3.88731E-5	45.0	9
CCGTTAC	20	7.029069E-4	45.0	44
ATACCGG	20	7.029069E-4	45.0	2
TATAGCG	30	2.1627802E-6	44.999996	1
TCGATCA	85	0.0	42.352943	17
TATGGGC	135	0.0	40.000004	4
CGAAAGG	90	0.0	40.0	2
TACGGGA	130	0.0	39.807693	4
TACGGGT	40	3.45246E-7	39.375	4
CGGTCTA	40	3.45246E-7	39.375	31
ATAACGC	40	3.45246E-7	39.375	11
CGTATGG	75	0.0	39.000004	2
TAGGGAC	260	0.0	38.94231	5
AGGTACG	35	6.240489E-6	38.571426	1
CGCATCG	35	6.240489E-6	38.571426	21
ATGGTCG	35	6.240489E-6	38.571426	44
>>END_MODULE