FastQCFastQC Report
Sat 18 Jun 2016
SRR3550351_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550351_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539803
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC13680.2534257868148195No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC10020.1856232736757669No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9570.17728689910948994No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT7700.1426446314674057No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAA207.029957E-445.00000410
TCCCGGT207.029957E-445.00000413
TATCGTG207.029957E-445.00000423
ATCGAGC207.029957E-445.00000442
CCGCTAT351.2103192E-745.025
GTAATCG253.888047E-545.01
AGTCGAC253.888047E-545.018
GCGTAAG700.041.7857131
TACGCGG603.6379788E-1241.2499962
CGTAAGG1000.040.52
CGATTCG403.4536788E-739.37500410
GGCATAT403.4536788E-739.3750048
TACACGG403.4536788E-739.3750042
AGCAACG1900.039.07894510
CGCACGG700.038.5714262
CGAGACA5750.038.34782422
AACGGGA2000.038.254
TAGATAC1650.038.1818220
GCGAGAC5800.038.0172421
GTCGACC301.1391199E-437.49999619