##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550351_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	539803
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2525513937492	33.0	31.0	34.0	30.0	34.0
2	32.3543533474249	34.0	31.0	34.0	30.0	34.0
3	32.46120343903239	34.0	31.0	34.0	30.0	34.0
4	35.94306070918465	37.0	35.0	37.0	35.0	37.0
5	35.916632549281864	37.0	35.0	37.0	35.0	37.0
6	35.9292056546555	37.0	35.0	37.0	35.0	37.0
7	36.133004077413425	37.0	35.0	37.0	35.0	37.0
8	36.12272625383705	37.0	35.0	37.0	35.0	37.0
9	37.71054810736509	39.0	38.0	39.0	35.0	39.0
10	37.40040533305669	39.0	37.0	39.0	35.0	39.0
11	37.329572084630875	39.0	37.0	39.0	34.0	39.0
12	37.214050310946774	39.0	37.0	39.0	34.0	39.0
13	37.14254088991725	39.0	37.0	39.0	33.0	39.0
14	38.31040027565612	40.0	38.0	41.0	34.0	41.0
15	38.40174100551498	40.0	38.0	41.0	34.0	41.0
16	38.4326059692147	40.0	38.0	41.0	34.0	41.0
17	38.42963266228605	40.0	38.0	41.0	34.0	41.0
18	38.389575456231256	40.0	37.0	41.0	34.0	41.0
19	38.359985031576336	40.0	37.0	41.0	34.0	41.0
20	38.274320446533274	40.0	37.0	41.0	34.0	41.0
21	38.22167716741107	40.0	37.0	41.0	34.0	41.0
22	38.20118080114412	40.0	37.0	41.0	34.0	41.0
23	38.154537859181964	40.0	37.0	41.0	34.0	41.0
24	38.14198698414051	40.0	37.0	41.0	34.0	41.0
25	38.09883976191314	40.0	37.0	41.0	34.0	41.0
26	37.98229168789354	40.0	36.0	41.0	34.0	41.0
27	37.94903511095715	40.0	36.0	41.0	34.0	41.0
28	37.8624924277931	40.0	36.0	41.0	33.0	41.0
29	37.819484145141836	40.0	36.0	41.0	33.0	41.0
30	37.77822464862181	40.0	36.0	41.0	33.0	41.0
31	37.6659596186016	40.0	36.0	41.0	33.0	41.0
32	37.52628088395211	40.0	36.0	41.0	33.0	41.0
33	37.45169070938842	40.0	36.0	41.0	33.0	41.0
34	37.265982219439316	40.0	35.0	41.0	32.0	41.0
35	37.26958353325195	40.0	35.0	41.0	32.0	41.0
36	37.13502889016919	40.0	35.0	41.0	32.0	41.0
37	37.11923053410226	39.0	35.0	41.0	32.0	41.0
38	36.994092289224035	39.0	35.0	41.0	31.0	41.0
39	36.91966884215167	39.0	35.0	41.0	31.0	41.0
40	36.82547892471883	39.0	35.0	41.0	31.0	41.0
41	36.708421405586854	39.0	35.0	41.0	31.0	41.0
42	36.66134682467493	39.0	35.0	41.0	31.0	41.0
43	36.58546914337268	39.0	35.0	41.0	31.0	41.0
44	36.39860467615037	39.0	35.0	41.0	30.0	41.0
45	36.30058558400009	39.0	35.0	41.0	30.0	41.0
46	36.18670329731402	38.0	35.0	40.0	30.0	41.0
47	36.08937519798889	38.0	35.0	40.0	30.0	41.0
48	35.98110977523282	38.0	35.0	40.0	30.0	41.0
49	35.89509691498565	38.0	35.0	40.0	29.0	41.0
50	35.78791336839551	38.0	35.0	40.0	29.0	41.0
51	34.56065824013575	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	4.0
11	2.0
12	9.0
13	5.0
14	8.0
15	8.0
16	26.0
17	42.0
18	104.0
19	211.0
20	337.0
21	592.0
22	920.0
23	1412.0
24	2083.0
25	2863.0
26	3699.0
27	4617.0
28	5628.0
29	6762.0
30	8204.0
31	10126.0
32	13447.0
33	17933.0
34	32118.0
35	44243.0
36	39538.0
37	58614.0
38	103765.0
39	182351.0
40	128.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.83413949162935	20.308890465595784	24.803307873427897	11.053662169346966
2	32.56780714445826	23.430399608746153	24.48115330963333	19.52063993716226
3	33.339384923759226	24.429467787322412	25.047656274603884	17.18349101431448
4	28.26457059334609	26.178439171327316	25.358880925078225	20.19810931024837
5	26.14231488154012	30.35940889546742	23.36741366757873	20.130862555413735
6	27.325894817183304	34.78528277908793	22.767750457111205	15.121071946617562
7	83.29020031381819	5.239874546825416	7.19225347024748	4.277671669108916
8	83.68497396272345	4.571667812146283	7.136677639805633	4.606680585324646
9	76.9508505880849	7.2289335183390975	9.681680168505919	6.138535725070072
10	38.4468037413649	31.63598572071663	15.833368840113893	14.08384169780457
11	29.75604062963711	25.896113952682736	25.80941565719346	18.538429760486697
12	26.61081913216488	24.045253546201113	28.59709931215647	20.74682800947753
13	24.236249150152926	26.341276354521924	30.280861721776276	19.141612773548868
14	20.94282543816911	28.006513487327783	29.51687930596903	21.533781768534077
15	20.31944987337973	26.118046768913842	32.69100023527102	20.871503122435406
16	23.890937990340923	26.114341713551052	30.152296300687475	19.842423995420553
17	22.761451862994463	24.794230487789065	28.03745069960708	24.406866949609395
18	24.685301860123047	24.03951071038879	28.58246434347345	22.692723086014713
19	23.372230239550355	27.087289251819648	26.871655029705284	22.66882547892472
20	26.84590489493389	26.553205521273505	26.57247180916001	20.028417774632597
21	25.33368654861125	26.88295544856179	27.017078452694783	20.76627955013218
22	24.168261384245735	24.30405166329198	26.610263373860466	24.917423578601824
23	22.234037232101343	28.2406729862561	27.39332682478608	22.131962956856484
24	22.795353119563988	24.999120049351337	29.366454058239768	22.839072772844908
25	22.60065246024939	26.989661043010134	26.80885434130599	23.60083215543448
26	22.58824052478404	27.525226795701396	27.336268972199118	22.550263707315445
27	23.09453634010926	25.96076716876342	27.013929155636408	23.93076733549091
28	20.37187640676321	27.596363858666955	28.69491277373412	23.33684696083571
29	23.234402180054577	25.510602942184462	28.006328234559646	23.248666643201314
30	24.096383310207614	24.72624272188187	27.89239778215386	23.284976185756655
31	24.098050585120866	26.441127596549112	26.224567110594048	23.23625470773597
32	23.48541968088358	26.33757129915914	26.897034658940388	23.27997436101689
33	22.171977554774614	26.754575280241127	26.194556162155454	24.87889100282881
34	20.715520291661957	27.619705707452535	29.448706287293696	22.216067713591812
35	21.22515065681369	26.609707615556044	28.035968677461966	24.1291730501683
36	21.685503785640318	30.364410720207186	25.362400727672874	22.587684766479622
37	21.256273121861124	27.99428680463058	27.430006872877698	23.319433200630602
38	20.368541856936698	28.847931560217337	27.044866367915706	23.738660214930263
39	22.710507351756103	27.089697537805456	26.49633292145468	23.70346218898376
40	24.051181634781578	25.849986013416004	27.51318536577233	22.58564698603009
41	20.47672947353016	27.397217132917007	25.980774467722483	26.14527892583035
42	23.170304722278313	26.024308868235263	26.299779734458685	24.505606675027742
43	22.88205141505327	25.33535382352451	27.764388119369478	24.01820664205275
44	22.66938123722914	25.687889841293952	27.14731114869684	24.49541777278007
45	22.673827303664485	24.5574774501068	27.035418476740592	25.73327676948813
46	23.23236439960504	25.672328608770233	26.518933759167695	24.576373232457026
47	21.00118006013305	26.94408886204782	28.32255470977375	23.73217636804538
48	21.748489726807744	25.075999948129223	28.505213939159287	24.670296385903747
49	22.92132500189884	25.414642008288208	28.375907506997923	23.28812548281503
50	20.72218939131498	25.371848618847988	28.82940628340339	25.076555706433645
51	20.673282660526155	24.566369582977494	26.349427476320063	28.41092028017629
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	208.0
1	413.5
2	619.0
3	1300.0
4	1981.0
5	1400.0
6	819.0
7	835.0
8	851.0
9	890.0
10	929.0
11	1026.5
12	1124.0
13	1052.0
14	980.0
15	1001.0
16	1022.0
17	1016.5
18	1011.0
19	1088.0
20	1165.0
21	1288.5
22	1412.0
23	1735.0
24	2058.0
25	2440.0
26	3435.5
27	4049.0
28	4585.5
29	5122.0
30	6803.5
31	8485.0
32	9190.0
33	9895.0
34	11069.0
35	12243.0
36	14327.0
37	16411.0
38	17086.5
39	17762.0
40	20870.0
41	23978.0
42	27947.5
43	31917.0
44	36631.5
45	41346.0
46	46952.0
47	52558.0
48	52821.5
49	53085.0
50	48957.5
51	44830.0
52	41398.0
53	37966.0
54	34916.5
55	31867.0
56	29681.0
57	27495.0
58	25602.5
59	23710.0
60	22634.5
61	21559.0
62	19770.0
63	17981.0
64	15574.5
65	13168.0
66	10855.0
67	8542.0
68	7043.5
69	5545.0
70	4623.5
71	3702.0
72	3579.0
73	3456.0
74	3170.0
75	2230.5
76	1577.0
77	1193.5
78	810.0
79	618.5
80	427.0
81	315.0
82	203.0
83	167.0
84	131.0
85	91.5
86	52.0
87	39.0
88	26.0
89	18.0
90	10.0
91	7.0
92	4.0
93	4.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	539803.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.39331468456456
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.20175879393128	23.388542933908425
2	9.964050415637997	6.45537241293256
3	3.5089927438597854	3.4100371853311104
4	1.762972365328419	2.284340744410984
5	1.1697524481353565	1.8946079577744197
6	0.9174747823015368	1.7832029602947628
7	0.7696837884126577	1.7452826415961322
8	0.6973194124776079	1.807078973123426
9	0.6293268511190012	1.8347384454969529
>10	8.25466269869444	50.81504867359104
>50	0.08324456719806901	1.829946470104131
>100	0.03846473105014212	1.9898808160685122
>500	0.0011482009268699142	0.32117048311685237
>1k	0.0011482009268699142	0.44074930225068915
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	1368	0.2534257868148195	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	1002	0.1856232736757669	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	957	0.17728689910948994	No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT	770	0.1426446314674057	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8525276813948792E-4	0.0	0.0	0.16061414997693604	0.0
2	1.8525276813948792E-4	0.0	0.0	0.8049232775660751	0.0
3	1.8525276813948792E-4	0.0	0.0	1.1517164595231963	0.0
4	1.8525276813948792E-4	0.0	0.0	1.564644879706115	0.0
5	1.8525276813948792E-4	0.0	0.0	2.7758274778020873	0.0
6	1.8525276813948792E-4	0.0	0.0	3.578527722150488	0.0
7	1.8525276813948792E-4	0.0	0.0	4.3095351452289075	0.0
8	1.8525276813948792E-4	0.0	0.0	5.616678677221135	0.0
9	1.8525276813948792E-4	0.0	0.0	6.105560732341243	0.0
10	1.8525276813948792E-4	0.0	0.0	7.125377220949124	0.0
11	1.8525276813948792E-4	0.0	0.0	8.459382404321577	0.0
12	3.7050553627897585E-4	0.0	0.0	9.450855219404115	0.0
13	3.7050553627897585E-4	0.0	0.0	9.860449089760523	0.0
14	3.7050553627897585E-4	0.0	0.0	10.03217840582583	0.0
15	3.7050553627897585E-4	0.0	0.0	10.387863720653646	0.0
16	3.7050553627897585E-4	0.0	0.0	11.079412304118355	0.0
17	3.7050553627897585E-4	0.0	0.0	11.93083402648744	0.0
18	5.557583044184638E-4	0.0	0.0	12.84653845940093	0.0
19	5.557583044184638E-4	0.0	0.0	13.376546629048004	0.0
20	7.410110725579517E-4	0.0	0.0	13.89951519350578	0.0
21	7.410110725579517E-4	0.0	0.0	14.547529376457708	0.0
22	7.410110725579517E-4	0.0	0.0	15.215180352832423	0.0
23	7.410110725579517E-4	0.0	0.0	15.90746994736969	0.0
24	7.410110725579517E-4	0.0	0.0	16.43469932549467	0.0
25	7.410110725579517E-4	0.0	0.0	16.914133489439667	0.0
26	7.410110725579517E-4	0.0	0.0	17.365594485395597	0.0
27	7.410110725579517E-4	0.0	0.0	17.834469241556643	0.0
28	7.410110725579517E-4	0.0	0.0	18.299638942354896	0.0
29	7.410110725579517E-4	0.0	0.0	18.805193746607557	0.0
30	7.410110725579517E-4	0.0	0.0	19.396335329740666	0.0
31	7.410110725579517E-4	0.0	0.0	19.897629320326118	0.0
32	7.410110725579517E-4	0.0	0.0	20.406148168869013	0.0
33	7.410110725579517E-4	0.0	0.0	20.887064354959126	0.0
34	7.410110725579517E-4	0.0	0.0	21.368165793817376	0.0
35	7.410110725579517E-4	0.0	0.0	21.86593998180818	0.0
36	7.410110725579517E-4	0.0	0.0	22.34333636530364	0.0
37	7.410110725579517E-4	0.0	0.0	22.821473759871658	0.0
38	7.410110725579517E-4	0.0	0.0	23.332586147168502	0.0
39	7.410110725579517E-4	0.0	0.0	23.828137301941634	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGAA	20	7.029957E-4	45.000004	10
TCCCGGT	20	7.029957E-4	45.000004	13
TATCGTG	20	7.029957E-4	45.000004	23
ATCGAGC	20	7.029957E-4	45.000004	42
CCGCTAT	35	1.2103192E-7	45.0	25
GTAATCG	25	3.888047E-5	45.0	1
AGTCGAC	25	3.888047E-5	45.0	18
GCGTAAG	70	0.0	41.785713	1
TACGCGG	60	3.6379788E-12	41.249996	2
CGTAAGG	100	0.0	40.5	2
CGATTCG	40	3.4536788E-7	39.375004	10
GGCATAT	40	3.4536788E-7	39.375004	8
TACACGG	40	3.4536788E-7	39.375004	2
AGCAACG	190	0.0	39.078945	10
CGCACGG	70	0.0	38.571426	2
CGAGACA	575	0.0	38.347824	22
AACGGGA	200	0.0	38.25	4
TAGATAC	165	0.0	38.18182	20
GCGAGAC	580	0.0	38.01724	21
GTCGACC	30	1.1391199E-4	37.499996	19
>>END_MODULE