FastQCFastQC Report
Sat 18 Jun 2016
SRR3550350_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550350_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678822
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102231.5059912613321311No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT38140.5618556852901054No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG35580.5241432952968526No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTC30190.44474103667824555No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCT23640.34825035134394583No Hit
CGTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT16720.24630904714343377No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC13660.20123095597962354TruSeq Adapter, Index 22 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT10230.1507022459496009TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTC9950.14657745329408886No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC7290.10739192306672442TruSeq Adapter, Index 22 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG207.031242E-445.00000421
TGATTCG502.1827873E-1145.00000415
TATAGCG207.031242E-445.0000041
TGTACGA207.031242E-445.00000427
GCGTAAG351.2109376E-745.01
CGTTTTT66950.043.8573571
CTATGCG501.0804797E-940.5000041
CGCCGGT451.9263098E-840.028
CGTTCTG4300.039.767441
CGTTTTC3750.039.6000021
ACGGGTA1250.039.65
AACGCCG403.455425E-739.37500413
ACGTAGG403.455425E-739.3750042
CGTTTCT4600.039.1304361
CCGTTGG356.2445106E-638.5714262
TAGTACG356.2445106E-638.5714261
TAGGGCG2700.038.3333325
GTTTTTT78600.037.843512
AACGTAG301.1394295E-437.5000041
TTAAGCG301.1394295E-437.5000041
Produced by FastQC (version 0.11.5)