##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550350_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	678822
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.197972369781766	33.0	31.0	34.0	30.0	34.0
2	32.35401916850073	34.0	31.0	34.0	30.0	34.0
3	32.31879491236289	34.0	31.0	34.0	30.0	34.0
4	35.90727318796385	37.0	35.0	37.0	35.0	37.0
5	35.90635247531753	37.0	35.0	37.0	35.0	37.0
6	35.94251364864427	37.0	35.0	37.0	35.0	37.0
7	36.113996010736244	37.0	35.0	37.0	35.0	37.0
8	36.12574135782282	37.0	36.0	37.0	35.0	37.0
9	37.760090274033544	39.0	38.0	39.0	35.0	39.0
10	37.43994154579551	39.0	37.0	39.0	35.0	39.0
11	37.436137897711035	39.0	37.0	39.0	35.0	39.0
12	37.38526005344553	39.0	37.0	39.0	35.0	39.0
13	37.367303947131944	39.0	37.0	39.0	34.0	39.0
14	38.62774335540097	40.0	38.0	41.0	34.0	41.0
15	38.678129170828285	40.0	38.0	41.0	35.0	41.0
16	38.66873937497606	40.0	38.0	41.0	35.0	41.0
17	38.69450901709137	40.0	38.0	41.0	35.0	41.0
18	38.48636166771259	40.0	38.0	41.0	35.0	41.0
19	38.26993968963882	40.0	37.0	41.0	34.0	41.0
20	38.01522343117931	40.0	35.0	41.0	34.0	41.0
21	37.98213964780163	40.0	35.0	41.0	34.0	41.0
22	37.95865631932966	40.0	35.0	41.0	34.0	41.0
23	37.918323212859924	40.0	35.0	41.0	34.0	41.0
24	37.8912572073386	40.0	35.0	41.0	34.0	41.0
25	37.8531411769212	40.0	35.0	41.0	34.0	41.0
26	37.73136109318791	40.0	35.0	41.0	33.0	41.0
27	37.66095088255832	40.0	35.0	41.0	33.0	41.0
28	37.565503180509765	40.0	35.0	41.0	33.0	41.0
29	37.466038225042794	39.0	35.0	41.0	33.0	41.0
30	37.36271953472339	39.0	35.0	41.0	33.0	41.0
31	37.13380238118388	39.0	35.0	41.0	33.0	41.0
32	36.740985118337356	39.0	35.0	41.0	31.0	41.0
33	36.32936911296334	39.0	35.0	41.0	30.0	41.0
34	35.89613035523303	39.0	35.0	41.0	27.0	41.0
35	35.66991641402311	39.0	35.0	41.0	25.0	41.0
36	35.465261880139415	39.0	35.0	41.0	24.0	41.0
37	35.3604788884273	39.0	35.0	41.0	23.0	41.0
38	35.238635459663946	39.0	35.0	41.0	23.0	41.0
39	35.11600832029604	39.0	35.0	41.0	23.0	41.0
40	35.04672653508578	39.0	35.0	41.0	22.0	41.0
41	34.89539378511598	39.0	35.0	41.0	21.0	41.0
42	34.81549949765918	39.0	35.0	41.0	21.0	41.0
43	34.72613733791775	38.0	34.0	41.0	20.0	41.0
44	34.54971111720009	38.0	34.0	40.0	18.0	41.0
45	34.49486463314389	38.0	34.0	40.0	20.0	41.0
46	34.38824610870007	38.0	34.0	40.0	19.0	41.0
47	34.30579003037615	38.0	34.0	40.0	18.0	41.0
48	34.151477117712744	38.0	34.0	40.0	18.0	41.0
49	34.06532051112074	38.0	34.0	40.0	18.0	41.0
50	33.984530554401594	38.0	34.0	40.0	18.0	41.0
51	32.77557887045499	36.0	31.0	40.0	14.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	5.0
11	8.0
12	4.0
13	7.0
14	7.0
15	27.0
16	53.0
17	96.0
18	188.0
19	412.0
20	730.0
21	1215.0
22	1917.0
23	3114.0
24	5627.0
25	9963.0
26	14297.0
27	15282.0
28	13565.0
29	12340.0
30	12682.0
31	13880.0
32	16563.0
33	21479.0
34	35132.0
35	47230.0
36	48683.0
37	69278.0
38	119811.0
39	215056.0
40	168.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.47459275038228	18.806402856713543	21.708931059983325	21.010073332920854
2	40.5157758587671	21.632769709879764	22.246921873480822	15.604532557872314
3	28.103980130284523	21.700092218578657	34.40666330790693	15.789264343229888
4	24.436744831487488	23.987437060083497	34.49254738355563	17.083270724873383
5	22.545527398935214	28.15495078238478	32.13080896022816	17.168712858451848
6	23.132721096252038	31.819092486690177	31.43533945570415	13.61284696135364
7	73.35987932035202	5.145973465798103	16.784370571372172	4.709776642477704
8	74.60969149497217	4.19579801479622	16.603174322576464	4.591336167655144
9	68.52341261774073	6.24729310481982	18.704166924466207	6.525127352973239
10	34.61496533701029	22.120968383464295	27.281820565626923	15.982245713898488
11	26.10360300638459	23.728311692903294	31.1875572683266	18.980528032385514
12	22.45242493613937	21.720716181856215	35.68505440306885	20.14180447893557
13	21.032907006549582	21.976600640521372	37.930414747901516	19.060077605027534
14	19.62104940617717	25.044562492081873	36.29301937768664	19.041368724054315
15	19.42158621847848	24.23079982675871	38.0294097716338	18.31820418312901
16	21.09035947567993	23.70208979673611	36.33235222193741	18.875198505646544
17	21.252552215455598	23.817436677067036	33.891064225967924	21.03894688150944
18	21.271261096428816	24.183070083173497	34.866872317043345	19.67879650335434
19	20.764058913824243	26.728951035764897	32.71741340145134	19.78957664895952
20	21.5922583534417	25.243141795640096	33.028687932919084	20.13591191799912
21	20.599656463697404	26.125110853802617	33.806505976529934	19.46872670597005
22	20.430834592868234	23.762193918287856	33.34408725704235	22.46288423180156
23	19.376508127314672	25.926384236220983	33.05107966447758	21.646027971986765
24	19.830971889538052	24.04312176093291	34.61658579126781	21.509320558261223
25	21.13425905465645	25.4692688215762	32.17323539897057	21.223236724796777
26	19.95913508990575	25.876002840214372	33.10352345681194	21.06133861306793
27	20.913729961609963	24.35203926802608	33.1478649778587	21.58636579250525
28	19.705313027568348	24.600852653567504	33.66523182807864	22.028602490785506
29	20.315340398513897	23.18472294651617	34.12485158112141	22.37508507384852
30	21.010073332920854	23.36591919531188	33.59894051754363	22.02506695422364
31	20.306354243085817	24.680696854256347	32.793280123508076	22.219668779149764
32	20.212073268102685	24.76260345127294	33.66626302624252	21.359060254381856
33	20.72531532566711	25.10923334835936	31.89908400140243	22.266367324571096
34	20.859813029041486	24.395791532979192	33.17673852644729	21.567656911532037
35	20.445565995209346	24.639596241724636	33.10470196899924	21.81013579406678
36	21.70937300205356	25.099363308790817	31.620513183131955	21.57075050602367
37	21.905006025143557	25.85184334037494	32.22243828278989	20.020712351691607
38	21.542318899505318	25.5564787234356	32.18074841416454	20.720453962894545
39	23.160710760700155	24.994475724122083	30.7976170483573	21.047196466820463
40	22.582650532834823	23.778987716956728	33.21386166034689	20.424500089861557
41	21.45245734522452	24.742421430065615	31.797584639272152	22.007536585437716
42	21.877458302765675	24.71251668331315	31.49279192483449	21.917233089086682
43	21.507552789980288	24.323460347484318	31.84354661457643	22.325440247958962
44	21.967614485093296	24.891945163827923	30.27082210063905	22.869618250439732
45	21.377916449378482	23.89772281982611	30.64205343963513	24.082307291160273
46	21.853004174879423	24.51968262666796	30.299548335204225	23.327764863248394
47	21.09315844212474	24.221224415236982	32.17529779529833	22.510319347339948
48	20.65946595720233	23.309350610322	32.46992584212062	23.561257590355055
49	20.983409494683436	23.18590145870346	31.822775337275456	24.007913709337647
50	19.93350244983221	22.58486024318599	33.35926060145369	24.122376705528108
51	20.604812454516797	22.51532802413593	31.656605118867688	25.22325440247959
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	366.0
1	561.5
2	757.0
3	8573.5
4	16390.0
5	11179.0
6	5968.0
7	5914.5
8	5861.0
9	6004.5
10	6148.0
11	6201.0
12	6254.0
13	6135.0
14	6016.0
15	5855.0
16	5694.0
17	5375.0
18	5056.0
19	4772.5
20	4489.0
21	4491.5
22	4494.0
23	4409.0
24	4324.0
25	4541.5
26	5020.5
27	5282.0
28	6001.5
29	6721.0
30	7684.0
31	8647.0
32	9548.0
33	10449.0
34	12102.5
35	13756.0
36	16301.0
37	18846.0
38	20576.0
39	22306.0
40	24878.5
41	27451.0
42	32735.0
43	38019.0
44	48035.0
45	58051.0
46	64294.5
47	70538.0
48	69129.5
49	67721.0
50	58963.0
51	50205.0
52	45011.5
53	39818.0
54	35923.5
55	32029.0
56	30185.5
57	28342.0
58	26223.5
59	24105.0
60	23121.0
61	22137.0
62	19359.5
63	16582.0
64	14561.5
65	12541.0
66	10790.0
67	9039.0
68	7772.0
69	6505.0
70	5549.5
71	4594.0
72	3938.0
73	3282.0
74	2727.0
75	1755.5
76	1339.0
77	1116.0
78	893.0
79	666.0
80	439.0
81	320.0
82	201.0
83	156.0
84	111.0
85	91.0
86	71.0
87	56.5
88	42.0
89	24.0
90	6.0
91	5.5
92	5.0
93	2.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	678822.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.227787978311554
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.5705114993237	31.911755370059964
2	8.864635707163638	7.486679142941519
3	3.604270488228444	4.566011099801886
4	2.2095978657022943	3.732257207608248
5	1.5532531933841138	3.279522326342985
6	1.194410677945986	3.026239252040068
7	0.9932648457269204	2.936036411816671
8	0.7896318663693039	2.6675525627169128
9	0.7141795672102951	2.714240100833878
>10	4.450232154232114	31.103668843453413
>50	0.03605781247722841	1.050918390699593
>100	0.015053260634154785	0.927480894609534
>500	0.002100454972207561	0.6004825301089928
>1k	0.0024505308009088213	2.4858971507417094
>5k	0.0	0.0
>10k+	3.5007582870126016E-4	1.5112587162245774
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10223	1.5059912613321311	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT	3814	0.5618556852901054	No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG	3558	0.5241432952968526	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTC	3019	0.44474103667824555	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCT	2364	0.34825035134394583	No Hit
CGTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT	1672	0.24630904714343377	No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC	1366	0.20123095597962354	TruSeq Adapter, Index 22 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT	1023	0.1507022459496009	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTC	995	0.14657745329408886	No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC	729	0.10739192306672442	TruSeq Adapter, Index 22 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.946280468222892E-4	0.0	0.0	0.16970575496963858	0.0
2	2.946280468222892E-4	0.0	0.0	0.5873410113402335	0.0
3	2.946280468222892E-4	0.0	0.0	1.3698731037002336	0.0
4	2.946280468222892E-4	0.0	0.0	1.9349696975053843	0.0
5	2.946280468222892E-4	0.0	0.0	3.3046954871822067	0.0
6	2.946280468222892E-4	0.0	0.0	4.98982060098229	0.0
7	2.946280468222892E-4	0.0	0.0	6.225785257401793	0.0
8	2.946280468222892E-4	0.0	0.0	7.85478372828223	0.0
9	2.946280468222892E-4	0.0	0.0	8.743087289451433	0.0
10	2.946280468222892E-4	0.0	0.0	9.99879202500803	0.0
11	2.946280468222892E-4	0.0	0.0	11.332720506995942	0.0
12	2.946280468222892E-4	0.0	0.0	12.388520112783617	0.0
13	2.946280468222892E-4	0.0	0.0	13.033313593254196	0.0
14	2.946280468222892E-4	0.0	0.0	13.373167045263706	0.0
15	2.946280468222892E-4	0.0	0.0	13.709926902781584	0.0
16	2.946280468222892E-4	0.0	0.0	14.274876182563323	0.0
17	2.946280468222892E-4	0.0	0.0	14.963421927987012	0.0
18	2.946280468222892E-4	0.0	0.0	15.689974691450779	0.0
19	2.946280468222892E-4	0.0	0.0	16.15637088957046	0.0
20	2.946280468222892E-4	0.0	0.0	16.627481136439304	0.0
21	2.946280468222892E-4	0.0	0.0	17.175931245598992	0.0
22	2.946280468222892E-4	0.0	0.0	17.769164817875673	0.0
23	2.946280468222892E-4	0.0	0.0	18.41381098432284	0.0
24	2.946280468222892E-4	0.0	0.0	18.944436096649785	0.0
25	2.946280468222892E-4	0.0	0.0	19.397574032662465	0.0
26	2.946280468222892E-4	0.0	0.0	19.854247505237012	0.0
27	2.946280468222892E-4	0.0	0.0	20.295894947423626	0.0
28	2.946280468222892E-4	0.0	0.0	20.773487011322555	0.0
29	2.946280468222892E-4	0.0	0.0	21.27332349275657	0.0
30	2.946280468222892E-4	0.0	0.0	21.86184301628409	0.0
31	4.419420702334338E-4	0.0	0.0	22.40115965599229	0.0
32	4.419420702334338E-4	0.0	0.0	22.90408973191794	0.0
33	4.419420702334338E-4	0.0	0.0	23.40348427128172	0.0
34	4.419420702334338E-4	0.0	0.0	23.85426518291982	0.0
35	4.419420702334338E-4	0.0	0.0	24.32994216451441	0.0
36	4.419420702334338E-4	0.0	0.0	24.798990015055494	0.0
37	4.419420702334338E-4	0.0	0.0	25.28276926793769	0.0
38	4.419420702334338E-4	0.0	0.0	25.788056368237918	0.0
39	4.419420702334338E-4	0.0	0.0	26.285388511273943	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	20	7.031242E-4	45.000004	21
TGATTCG	50	2.1827873E-11	45.000004	15
TATAGCG	20	7.031242E-4	45.000004	1
TGTACGA	20	7.031242E-4	45.000004	27
GCGTAAG	35	1.2109376E-7	45.0	1
CGTTTTT	6695	0.0	43.857357	1
CTATGCG	50	1.0804797E-9	40.500004	1
CGCCGGT	45	1.9263098E-8	40.0	28
CGTTCTG	430	0.0	39.76744	1
CGTTTTC	375	0.0	39.600002	1
ACGGGTA	125	0.0	39.6	5
AACGCCG	40	3.455425E-7	39.375004	13
ACGTAGG	40	3.455425E-7	39.375004	2
CGTTTCT	460	0.0	39.130436	1
CCGTTGG	35	6.2445106E-6	38.571426	2
TAGTACG	35	6.2445106E-6	38.571426	1
TAGGGCG	270	0.0	38.333332	5
GTTTTTT	7860	0.0	37.84351	2
AACGTAG	30	1.1394295E-4	37.500004	1
TTAAGCG	30	1.1394295E-4	37.500004	1
>>END_MODULE