FastQCFastQC Report
Sat 18 Jun 2016
SRR3550349_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550349_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences695391
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21670.31162324505206423No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC12010.17270859128173935TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC11520.1656621958006359TruSeq Adapter, Index 22 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT9890.1422221455267612Illumina Paired End PCR Primer 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAG302.164259E-645.0000041
TCACGAC406.8084773E-945.025
TACGAAT502.1827873E-1145.012
TATAGCG406.8084773E-945.01
CGCCCAT207.031363E-445.019
TACGTCG207.031363E-445.01
TAGTACG207.031363E-445.01
GCGAAAG700.041.7857131
TAGGGCG2100.041.7857135
GCTACGA556.002665E-1140.9090910
AACGGGC1600.039.3754
AAACACG7200.039.062540
CACGACC7550.039.03973427
GACACGA7550.038.74172225
CGTTTTT12350.038.6234781
AACACGT7050.038.61702341
CGGTCTA356.244729E-638.57142631
ATAACGG356.244729E-638.5714262
ACGACCA7600.038.48684328
GGCGATA1700.038.3823558
Produced by FastQC (version 0.11.5)